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The map label for this gene is fadD19_1

Identifier: 41406648

GI number: 41406648

Start: 575128

End: 576768

Strand: Direct

Name: fadD19_1

Synonym: MAP0550

Alternate gene names: 41406648

Gene position: 575128-576768 (Clockwise)

Preceding gene: 41406645

Following gene: 41406649

Centisome position: 11.91

GC content: 67.03

Gene sequence:

>1641_bases
GTGGCCGTGGCCCTGAATATTGCCGATCTCGCAGAGCACGCCATCGACGCTGTGCCCGACCGTGTCGCCCTGATCTGCGG
CGACGAGAAGCTGACCTATGCCGAGCTGGAGGAGAAGGCCAACCGCCTCGCCCACTACCTGCTCGACCAGGGGGTGAAGA
AGGACGACAAGGTCGGGCTGTACTGCCGCAACCGCAACGAGATCGTCATCGCCATGCTCGGCATCGTCAAGGCAGGCGCC
ATCCTGGTCAACGTCAACTACCGCTACGTCGAGGGCGAGCTGCGCTACCTGTTCGACAACTCCGACATGGTGGCGCTGGT
CCACGAGCGCCAGCACTCCGACCGGGTGGCCAACGTGCTGCCCGACACCCCCAACGTCAAGACCATCCTGGTGGTCGAGG
ACGGCAGCGACAAGGACTACCAGCGCTACGGCGGCGTCGAGTTCTACTCGGCGCTGGAAAAGGGCTCGCCCGAGCGGGAT
TTCGGCCCGCGCAGCGCCGACGACATCTACCTGCTCTACACCGGCGGCACCACCGGGTTCCCGAAGGGCGTGATGTGGCG
CCACGAGGACATCTACCGGGTGCTGTTCGGCGGAACCGACTTCGCCACAGGCGAATTCGTCAAGGACGAGTACGACCTGG
CCAAGGCCGCCGCCGAAAATCCGCCGATGATCCGCTACCCGATCCCGCCGATGATCCACGGCGCCACCCAGTCGGCCACC
TGGATGTCGATCTTCTCCGGCCAAACCACGGTGCTGGCACCGGAATTCAACGCCGACGAGGTGTGGCGCACCATCCACGA
GCACAAGGTCAACCTGCTGTTCTTCACCGGCGACGCGATGGCCCGCCCGCTGCTGGACGCGTTGAACAAGGACCACGACT
ACGACCTGTCGTCGCTGTTCCTGCTGGCCAGCACCGCGGCCCTGTTCTCGCCGAGCATCAAGGAGCGCCTGCTCGAGTTG
CTGCCCAACCGGGTCATCACCGACTCGATCGGCTCCTCGGAGACCGGGTTCGGCGGCACCAGCATCGTCGCCAAGGACGC
GCCGCACGCCGGCGGGCCGCGGGTGACGATCGACCACCGCACCGTGGTGCTCGACGAGGAGGGCAACGAGGTCAAGCCGG
GCTCCGGTGTGCGCGGGCTGATCGCCAAGAAGGGCAACATCCCGGTCGGCTACTACAAGGACGAGAAGAAGACCGCCGAG
ACGTTCAAGACGTTCAACGGCGTGCGCTACGCCATCCCCGGCGACTACGCACTGGTCGAGGAGGACGGCACGGTCACCAT
GCTGGGCCGCGGCTCGGTGTCGATCAACAGCGGCGGCGAGAAGATCTACCCCGAAGAGGTCGAGGGCGCGCTCAAGGGGC
ATCCCGACGTGTTCGACGCGCTGGTGGTCGGCGTGCCCGATCCGCGGTACGGCCAGCACGTGGCCGCCGTGGTGCAGCCC
CGGCCGGGGACCCGGCCGTCGCTGGCCGAGTTGGACCGCTTCGTGCGTTCGGAGATCGCGGGATACAAAGTGCCGCGTAG
TCTTTGGCTGGTCGATGAGGTGAAGCGGTCGCCGGCGGGCAAGCCCGACTACCGCTGGGCCAAGGAGCAGACCGAGGCCC
GCCCGGCCGACGACGTGCACGCCGCCCACGTCTCCGCCTGA

Upstream 100 bases:

>100_bases
GGGACCGGTTTTTTCGTTTGCTGACGCCTCGGCCACGGGTACCGCCTCTCACTCGACATCGGGCATGAGCTTGCCTCGAA
ATGTAACACGTTCTAATTTG

Downstream 100 bases:

>100_bases
TCCGGCTGGGTGACCGATTTCGTCAAGCAGCACCCCCGGTCGCCCGAGGGCTTCTTCGGGTGGGAGGCCGCCGGGTTGCG
CTGGCTGTCGAGCGTCGACG

Product: acyl-CoA synthetase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MAVALNIADLAEHAIDAVPDRVALICGDEKLTYAELEEKANRLAHYLLDQGVKKDDKVGLYCRNRNEIVIAMLGIVKAGA
ILVNVNYRYVEGELRYLFDNSDMVALVHERQHSDRVANVLPDTPNVKTILVVEDGSDKDYQRYGGVEFYSALEKGSPERD
FGPRSADDIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAENPPMIRYPIPPMIHGATQSAT
WMSIFSGQTTVLAPEFNADEVWRTIHEHKVNLLFFTGDAMARPLLDALNKDHDYDLSSLFLLASTAALFSPSIKERLLEL
LPNRVITDSIGSSETGFGGTSIVAKDAPHAGGPRVTIDHRTVVLDEEGNEVKPGSGVRGLIAKKGNIPVGYYKDEKKTAE
TFKTFNGVRYAIPGDYALVEEDGTVTMLGRGSVSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDPRYGQHVAAVVQP
RPGTRPSLAELDRFVRSEIAGYKVPRSLWLVDEVKRSPAGKPDYRWAKEQTEARPADDVHAAHVSA

Sequences:

>Translated_546_residues
MAVALNIADLAEHAIDAVPDRVALICGDEKLTYAELEEKANRLAHYLLDQGVKKDDKVGLYCRNRNEIVIAMLGIVKAGA
ILVNVNYRYVEGELRYLFDNSDMVALVHERQHSDRVANVLPDTPNVKTILVVEDGSDKDYQRYGGVEFYSALEKGSPERD
FGPRSADDIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAENPPMIRYPIPPMIHGATQSAT
WMSIFSGQTTVLAPEFNADEVWRTIHEHKVNLLFFTGDAMARPLLDALNKDHDYDLSSLFLLASTAALFSPSIKERLLEL
LPNRVITDSIGSSETGFGGTSIVAKDAPHAGGPRVTIDHRTVVLDEEGNEVKPGSGVRGLIAKKGNIPVGYYKDEKKTAE
TFKTFNGVRYAIPGDYALVEEDGTVTMLGRGSVSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDPRYGQHVAAVVQP
RPGTRPSLAELDRFVRSEIAGYKVPRSLWLVDEVKRSPAGKPDYRWAKEQTEARPADDVHAAHVSA
>Mature_545_residues
AVALNIADLAEHAIDAVPDRVALICGDEKLTYAELEEKANRLAHYLLDQGVKKDDKVGLYCRNRNEIVIAMLGIVKAGAI
LVNVNYRYVEGELRYLFDNSDMVALVHERQHSDRVANVLPDTPNVKTILVVEDGSDKDYQRYGGVEFYSALEKGSPERDF
GPRSADDIYLLYTGGTTGFPKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAENPPMIRYPIPPMIHGATQSATW
MSIFSGQTTVLAPEFNADEVWRTIHEHKVNLLFFTGDAMARPLLDALNKDHDYDLSSLFLLASTAALFSPSIKERLLELL
PNRVITDSIGSSETGFGGTSIVAKDAPHAGGPRVTIDHRTVVLDEEGNEVKPGSGVRGLIAKKGNIPVGYYKDEKKTAET
FKTFNGVRYAIPGDYALVEEDGTVTMLGRGSVSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDPRYGQHVAAVVQPR
PGTRPSLAELDRFVRSEIAGYKVPRSLWLVDEVKRSPAGKPDYRWAKEQTEARPADDVHAAHVSA

Specific function: Activates The Carboxylate Group Of 2,3-Dihydroxy- Benzoate (2,3-Dhb), Via ATP-Dependent Ppi Exchange Reactions, To The Acyladenylate. Then, Catalyzes The Acylation Of Holo-Entb With 2,3-Dhb Adenylate, Preparing That Molecule For Amide Bond Formation With

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=567, Percent_Identity=24.5149911816578, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI187761345, Length=554, Percent_Identity=25.2707581227437, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI187761343, Length=554, Percent_Identity=25.2707581227437, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=25.1461988304094, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI28416953, Length=567, Percent_Identity=25.7495590828924, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI58082049, Length=516, Percent_Identity=24.6124031007752, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=22.2003929273084, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4758332, Length=505, Percent_Identity=23.960396039604, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI12669909, Length=505, Percent_Identity=23.960396039604, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI157311624, Length=514, Percent_Identity=24.5136186770428, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157311622, Length=514, Percent_Identity=24.5136186770428, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42544132, Length=523, Percent_Identity=23.1357552581262, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI227499619, Length=528, Percent_Identity=22.9166666666667, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI122937307, Length=168, Percent_Identity=31.547619047619, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1786810, Length=533, Percent_Identity=25.891181988743, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788107, Length=562, Percent_Identity=25.8007117437722, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790505, Length=534, Percent_Identity=26.0299625468165, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI221142682, Length=525, Percent_Identity=25.5238095238095, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI145693145, Length=555, Percent_Identity=23.4234234234234, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1786529, Length=588, Percent_Identity=23.6394557823129, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71994694, Length=549, Percent_Identity=26.9581056466302, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=549, Percent_Identity=26.9581056466302, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=549, Percent_Identity=26.9581056466302, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=519, Percent_Identity=24.4701348747592, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=549, Percent_Identity=25.5009107468124, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=513, Percent_Identity=24.9512670565302, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=531, Percent_Identity=23.728813559322, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=539, Percent_Identity=24.860853432282, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=525, Percent_Identity=24.1904761904762, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=22.4528301886792, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983001, Length=538, Percent_Identity=25.2788104089219, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=538, Percent_Identity=25.2788104089219, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115534440, Length=547, Percent_Identity=22.3034734917733, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=545, Percent_Identity=24.0366972477064, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323182, Length=551, Percent_Identity=26.6787658802178, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319264, Length=557, Percent_Identity=25.673249551167, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581924, Length=533, Percent_Identity=24.015009380863, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI18859661, Length=536, Percent_Identity=25.5597014925373, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550496, Length=506, Percent_Identity=23.1225296442688, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45552801, Length=506, Percent_Identity=23.1225296442688, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281365686, Length=514, Percent_Identity=21.011673151751, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24583465, Length=496, Percent_Identity=24.3951612903226, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583463, Length=496, Percent_Identity=24.3951612903226, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364798, Length=496, Percent_Identity=24.3951612903226, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 60032; Mature: 59901

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVALNIADLAEHAIDAVPDRVALICGDEKLTYAELEEKANRLAHYLLDQGVKKDDKVGL
CEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YCRNRNEIVIAMLGIVKAGAILVNVNYRYVEGELRYLFDNSDMVALVHERQHSDRVANVL
EECCCCCEEEEEEHHHHCCEEEEEEEEEEEECEEEEEECCCCEEEEEECHHHHHHHHHCC
PDTPNVKTILVVEDGSDKDYQRYGGVEFYSALEKGSPERDFGPRSADDIYLLYTGGTTGF
CCCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCC
PKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAENPPMIRYPIPPMIHGATQSAT
CCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCE
WMSIFSGQTTVLAPEFNADEVWRTIHEHKVNLLFFTGDAMARPLLDALNKDHDYDLSSLF
EEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHH
LLASTAALFSPSIKERLLELLPNRVITDSIGSSETGFGGTSIVAKDAPHAGGPRVTIDHR
HHHHHHHHHCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEECE
TVVLDEEGNEVKPGSGVRGLIAKKGNIPVGYYKDEKKTAETFKTFNGVRYAIPGDYALVE
EEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCEEEECCCCEEEEE
EDGTVTMLGRGSVSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDPRYGQHVAAVVQP
CCCCEEEEECCEEEECCCCCEECHHHHHHHHCCCCCHHHEEEECCCCCCCCCEEEEEECC
RPGTRPSLAELDRFVRSEIAGYKVPRSLWLVDEVKRSPAGKPDYRWAKEQTEARPADDVH
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
AAHVSA
HHCCCC
>Mature Secondary Structure 
AVALNIADLAEHAIDAVPDRVALICGDEKLTYAELEEKANRLAHYLLDQGVKKDDKVGL
EEEEEHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YCRNRNEIVIAMLGIVKAGAILVNVNYRYVEGELRYLFDNSDMVALVHERQHSDRVANVL
EECCCCCEEEEEEHHHHCCEEEEEEEEEEEECEEEEEECCCCEEEEEECHHHHHHHHHCC
PDTPNVKTILVVEDGSDKDYQRYGGVEFYSALEKGSPERDFGPRSADDIYLLYTGGTTGF
CCCCCEEEEEEEECCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCC
PKGVMWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAENPPMIRYPIPPMIHGATQSAT
CCCCEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCE
WMSIFSGQTTVLAPEFNADEVWRTIHEHKVNLLFFTGDAMARPLLDALNKDHDYDLSSLF
EEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHH
LLASTAALFSPSIKERLLELLPNRVITDSIGSSETGFGGTSIVAKDAPHAGGPRVTIDHR
HHHHHHHHHCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEECE
TVVLDEEGNEVKPGSGVRGLIAKKGNIPVGYYKDEKKTAETFKTFNGVRYAIPGDYALVE
EEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCEEEECCCCEEEEE
EDGTVTMLGRGSVSINSGGEKIYPEEVEGALKGHPDVFDALVVGVPDPRYGQHVAAVVQP
CCCCEEEEECCEEEECCCCCEECHHHHHHHHCCCCCHHHEEEECCCCCCCCCEEEEEECC
RPGTRPSLAELDRFVRSEIAGYKVPRSLWLVDEVKRSPAGKPDYRWAKEQTEARPADDVH
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
AAHVSA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]