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The map label for this gene is fadD32

Identifier: 41406317

GI number: 41406317

Start: 226557

End: 228455

Strand: Direct

Name: fadD32

Synonym: MAP0219

Alternate gene names: 41406317

Gene position: 226557-228455 (Clockwise)

Preceding gene: 41406316

Following gene: 41406318

Centisome position: 4.69

GC content: 68.3

Gene sequence:

>1899_bases
ATGGCGTACCACAACCCGTTCATCGCGAATGGAAAGATCAAGTTCCCCGAGAACACGAACTTGGTCAAGCACGTTGAGAA
ATGGGCGAGGGTTCGTGGCGACAAGCTCGCCTACCGATTCCTGGACTTCTCCACCGAGCGCGACGGCGTCGCCTGCGACA
TCTCCTGGTCGGAGTTCAGCGCCCGCAACCGCGCGGTGGGCGCCCGGCTGCAGCAGGTCACCGAGCCCGGCGACCGCATC
GCGGTGCTGTGCCCGCAGAACCTGGACTACCTGATCGCGCTGTTCGGCGCCCTGTACGCCGGCCGGATCGCGGTGCCGCT
GTTCGACCCCAGCGAGCCCGGTCACGTCGGCCGGCTGCACGCCGTTCTCGACGACTGCACCCCGTCGACCATCCTGACCA
CCACCGAGGCCGCCGAGGGCGTCCGCGAGTTCATCCGCGCCCGCTCGGCCAAGGAGCGCCCGCGCGTCATCGCCGTCGAC
GCGGTGCCCAACGAGGTCAATTCCACCTGGGTGCCGCCGGAGGCCGACGAGAACACCATCGCCTACCTGCAGTACACCTC
CGGCTCCACCCGCACCCCGACCGGCGTGGAGATCACCCACCTGAACCTGCCCACCAACGTGCTGCAGGTGCTCAACGGGC
TGGAGGGCAAGGAGGGCGACCGCGGCCTGTCCTGGCTGCCGTTCTTCCACGACATGGGGTTGATCACCGCGATGCTGTCG
CCGGTGCTCGGCCACAACTTCACCTTCATGACCCCCGCCGCGTTCGTGCGCCGTCCCGGCCGGTGGATCCGGGAGATGGC
CCGCAAGCCCGACGACGCCCCCGACTGCGAGGTGTTCACCGTCGCGCCCAACTTCGCGTTCGAGCACGCCGCCGTGCGCG
GGGTGCCCAAGGAGGGCGAGCCGCCGCTGGACCTGAGCAACGTCAAGGGAATCCTGAACGGCAGCGAGCCGGTGTCCCCG
TCCTCGATGCGCAAGTTCTACGAGGCCTTCAAGCCGTACGGCCTGCGCGAGACCGCGATCAAGCCGTCCTACGGCCTGGC
CGAGGCGACGCTGTTCGTCTCCACCACCCCGATGGACCAGGCGCCCACCGTCATCCACGTCGACCGCGCCGAGCTGAACA
AGCAGCGCTTCGTCGAGGTGCCCGCCGATGCCCCCAACGCGGTGGCCCAGGTGTCCGCCGGCGTCATCGGCGTCGACGAG
TGGGCGGTCATCGTCGACCCGGAGACCGCCAGCGAGCTGCCGGACGGGCACATCGGCGAGATCTGGTTGCACGGCAACAA
CATGGGCATCGGCTACTGGGGCAAGGAAGAGGAGACCAACGAGGTCTTCCGCAACATCCTCAAGTCGCGGATCAGCCAGT
CGCACGCCGAGGGCGCCCCGGACGACGCGATGTGGGTCAAGACAGGCGACTACGGCACCTATTACAAGGGCCACCTCTAC
ATCGCCGGCCGGATCAAGGACCTGGTCATCATCGACGGCCGCAACCACTACCCGCAGGACCTGGAGTACTCGGCGCAGGA
GGCCAGCAAGGCGCTGCGCACCGGGTATGTGGCCGCCTTCTCGGTGCCGGCCAACCAGCTGCCCAAAGAGGTGTTCGACA
ACCCGCATACCGGGCTGAAGTATGACCCGGACGACACCTCCGAGCAGTTGGTGATCGTCGCCGAGCGCGCGCCCGGCACC
CACAAGCTGGACTACCAGCCGATCGCCGACGACATCCGGGCGGCCATCGCGGTGCGCCACGGCGTCACGGTCCGCGATCT
GCTGTTGGTGCAGGCGGGGACGATTCCGCGGACCTCCAGCGGCAAGATCGGGCACCGCGCCTGCCGCGCCGCCTACCTCG
ACGGCAGCCTGCGAAGCGGTGTCGGCTCCCCGACGGCCTTCGCCAACTCAACCGACTGA

Upstream 100 bases:

>100_bases
CCGTGCGTGGACATTGCGTAGACATGTAGCCGTCGGCGCGCGCCGACCGGACAACAACGTCGGCCTGGCCGACCGAACAC
AGGTGTGACAGGAGAGCGGA

Downstream 100 bases:

>100_bases
GCATCAGGACCATGGCTGACATAGACGAACCTCAGGAGAACACGCCCGCCAAGAAGAGCGATATGCGCGCCGGCGACGAT
GCAGAGCGAAGCGATGCTGA

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MAYHNPFIANGKIKFPENTNLVKHVEKWARVRGDKLAYRFLDFSTERDGVACDISWSEFSARNRAVGARLQQVTEPGDRI
AVLCPQNLDYLIALFGALYAGRIAVPLFDPSEPGHVGRLHAVLDDCTPSTILTTTEAAEGVREFIRARSAKERPRVIAVD
AVPNEVNSTWVPPEADENTIAYLQYTSGSTRTPTGVEITHLNLPTNVLQVLNGLEGKEGDRGLSWLPFFHDMGLITAMLS
PVLGHNFTFMTPAAFVRRPGRWIREMARKPDDAPDCEVFTVAPNFAFEHAAVRGVPKEGEPPLDLSNVKGILNGSEPVSP
SSMRKFYEAFKPYGLRETAIKPSYGLAEATLFVSTTPMDQAPTVIHVDRAELNKQRFVEVPADAPNAVAQVSAGVIGVDE
WAVIVDPETASELPDGHIGEIWLHGNNMGIGYWGKEEETNEVFRNILKSRISQSHAEGAPDDAMWVKTGDYGTYYKGHLY
IAGRIKDLVIIDGRNHYPQDLEYSAQEASKALRTGYVAAFSVPANQLPKEVFDNPHTGLKYDPDDTSEQLVIVAERAPGT
HKLDYQPIADDIRAAIAVRHGVTVRDLLLVQAGTIPRTSSGKIGHRACRAAYLDGSLRSGVGSPTAFANSTD

Sequences:

>Translated_632_residues
MAYHNPFIANGKIKFPENTNLVKHVEKWARVRGDKLAYRFLDFSTERDGVACDISWSEFSARNRAVGARLQQVTEPGDRI
AVLCPQNLDYLIALFGALYAGRIAVPLFDPSEPGHVGRLHAVLDDCTPSTILTTTEAAEGVREFIRARSAKERPRVIAVD
AVPNEVNSTWVPPEADENTIAYLQYTSGSTRTPTGVEITHLNLPTNVLQVLNGLEGKEGDRGLSWLPFFHDMGLITAMLS
PVLGHNFTFMTPAAFVRRPGRWIREMARKPDDAPDCEVFTVAPNFAFEHAAVRGVPKEGEPPLDLSNVKGILNGSEPVSP
SSMRKFYEAFKPYGLRETAIKPSYGLAEATLFVSTTPMDQAPTVIHVDRAELNKQRFVEVPADAPNAVAQVSAGVIGVDE
WAVIVDPETASELPDGHIGEIWLHGNNMGIGYWGKEEETNEVFRNILKSRISQSHAEGAPDDAMWVKTGDYGTYYKGHLY
IAGRIKDLVIIDGRNHYPQDLEYSAQEASKALRTGYVAAFSVPANQLPKEVFDNPHTGLKYDPDDTSEQLVIVAERAPGT
HKLDYQPIADDIRAAIAVRHGVTVRDLLLVQAGTIPRTSSGKIGHRACRAAYLDGSLRSGVGSPTAFANSTD
>Mature_631_residues
AYHNPFIANGKIKFPENTNLVKHVEKWARVRGDKLAYRFLDFSTERDGVACDISWSEFSARNRAVGARLQQVTEPGDRIA
VLCPQNLDYLIALFGALYAGRIAVPLFDPSEPGHVGRLHAVLDDCTPSTILTTTEAAEGVREFIRARSAKERPRVIAVDA
VPNEVNSTWVPPEADENTIAYLQYTSGSTRTPTGVEITHLNLPTNVLQVLNGLEGKEGDRGLSWLPFFHDMGLITAMLSP
VLGHNFTFMTPAAFVRRPGRWIREMARKPDDAPDCEVFTVAPNFAFEHAAVRGVPKEGEPPLDLSNVKGILNGSEPVSPS
SMRKFYEAFKPYGLRETAIKPSYGLAEATLFVSTTPMDQAPTVIHVDRAELNKQRFVEVPADAPNAVAQVSAGVIGVDEW
AVIVDPETASELPDGHIGEIWLHGNNMGIGYWGKEEETNEVFRNILKSRISQSHAEGAPDDAMWVKTGDYGTYYKGHLYI
AGRIKDLVIIDGRNHYPQDLEYSAQEASKALRTGYVAAFSVPANQLPKEVFDNPHTGLKYDPDDTSEQLVIVAERAPGTH
KLDYQPIADDIRAAIAVRHGVTVRDLLLVQAGTIPRTSSGKIGHRACRAAYLDGSLRSGVGSPTAFANSTD

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=577, Percent_Identity=25.1299826689775, Blast_Score=110, Evalue=6e-24,
Organism=Homo sapiens, GI44888818, Length=524, Percent_Identity=22.3282442748092, Blast_Score=109, Evalue=1e-23,
Organism=Homo sapiens, GI45827692, Length=569, Percent_Identity=23.1985940246046, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI225735629, Length=569, Percent_Identity=23.1985940246046, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788107, Length=599, Percent_Identity=25.7095158597663, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193202679, Length=586, Percent_Identity=23.3788395904437, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24654656, Length=444, Percent_Identity=22.2972972972973, Blast_Score=97, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 69321; Mature: 69190

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYHNPFIANGKIKFPENTNLVKHVEKWARVRGDKLAYRFLDFSTERDGVACDISWSEFS
CCCCCCEEECCEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEHHHHH
ARNRAVGARLQQVTEPGDRIAVLCPQNLDYLIALFGALYAGRIAVPLFDPSEPGHVGRLH
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHH
AVLDDCTPSTILTTTEAAEGVREFIRARSAKERPRVIAVDAVPNEVNSTWVPPEADENTI
HHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
AYLQYTSGSTRTPTGVEITHLNLPTNVLQVLNGLEGKEGDRGLSWLPFFHDMGLITAMLS
EEEEECCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHH
PVLGHNFTFMTPAAFVRRPGRWIREMARKPDDAPDCEVFTVAPNFAFEHAAVRGVPKEGE
HHHCCCCEEECCHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCC
PPLDLSNVKGILNGSEPVSPSSMRKFYEAFKPYGLRETAIKPSYGLAEATLFVSTTPMDQ
CCCCHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEEEECCCCC
APTVIHVDRAELNKQRFVEVPADAPNAVAQVSAGVIGVDEWAVIVDPETASELPDGHIGE
CCCEEEECHHHCCCCCEEECCCCCCHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCEE
IWLHGNNMGIGYWGKEEETNEVFRNILKSRISQSHAEGAPDDAMWVKTGDYGTYYKGHLY
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEE
IAGRIKDLVIIDGRNHYPQDLEYSAQEASKALRTGYVAAFSVPANQLPKEVFDNPHTGLK
EEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHCCHHHHCCCCCCCE
YDPDDTSEQLVIVAERAPGTHKLDYQPIADDIRAAIAVRHGVTVRDLLLVQAGTIPRTSS
ECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCCC
GKIGHRACRAAYLDGSLRSGVGSPTAFANSTD
CCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYHNPFIANGKIKFPENTNLVKHVEKWARVRGDKLAYRFLDFSTERDGVACDISWSEFS
CCCCCEEECCEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEHHHHH
ARNRAVGARLQQVTEPGDRIAVLCPQNLDYLIALFGALYAGRIAVPLFDPSEPGHVGRLH
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHH
AVLDDCTPSTILTTTEAAEGVREFIRARSAKERPRVIAVDAVPNEVNSTWVPPEADENTI
HHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
AYLQYTSGSTRTPTGVEITHLNLPTNVLQVLNGLEGKEGDRGLSWLPFFHDMGLITAMLS
EEEEECCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHH
PVLGHNFTFMTPAAFVRRPGRWIREMARKPDDAPDCEVFTVAPNFAFEHAAVRGVPKEGE
HHHCCCCEEECCHHHHHCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCC
PPLDLSNVKGILNGSEPVSPSSMRKFYEAFKPYGLRETAIKPSYGLAEATLFVSTTPMDQ
CCCCHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEEEEECCCCC
APTVIHVDRAELNKQRFVEVPADAPNAVAQVSAGVIGVDEWAVIVDPETASELPDGHIGE
CCCEEEECHHHCCCCCEEECCCCCCHHHHHHHCCEEEECCEEEEECCCHHHHCCCCCCEE
IWLHGNNMGIGYWGKEEETNEVFRNILKSRISQSHAEGAPDDAMWVKTGDYGTYYKGHLY
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEE
IAGRIKDLVIIDGRNHYPQDLEYSAQEASKALRTGYVAAFSVPANQLPKEVFDNPHTGLK
EEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHCCHHHHCCCCCCCE
YDPDDTSEQLVIVAERAPGTHKLDYQPIADDIRAAIAVRHGVTVRDLLLVQAGTIPRTSS
ECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCCC
GKIGHRACRAAYLDGSLRSGVGSPTAFANSTD
CCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]