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The map label for this gene is gltD

Identifier: 41406271

GI number: 41406271

Start: 180940

End: 182415

Strand: Direct

Name: gltD

Synonym: MAP0173

Alternate gene names: 41406271

Gene position: 180940-182415 (Clockwise)

Preceding gene: 41406270

Following gene: 41406272

Centisome position: 3.75

GC content: 69.38

Gene sequence:

>1476_bases
ATGGCTGATCCGAGCGGCTTCCTGAAATACACGCATCGGGAACTGCCGAAGCGCCGGCCTGTCCCGCTGCGGTTGCGCGA
CTGGCGCGAGGTCTACGAGGAATTCGACCACGGGACCCTGCGCGAGCAGGCCACTCGATGCATGGACTGCGGTATTCCGT
TCTGCCACAACGGTTGTCCGCTGGGCAACCTGATCCCGGAGTGGAATGACCTGGTGCGCACCGGGCGGTGGCGCGACGCC
ATCGAGCGGCTGCACGCCACCAACAACTTCCCGGACTTCACCGGGCGGCTGTGCCCAGCTCCGTGCGAGCCGGCCTGCGT
GCTCGGCATCAACCAGGACCCGGTGACGATCAAGCAGATCGAGCTGGAGATCATCGACAACGCCTTCGACGAGGGCTGGG
TGCAGCCGCTGCCGCCAGACAAGCTGACCGGCAAGAAGGTCGCCGTGGTCGGCTCCGGCCCGGCGGGTCTGGCCGCCGCC
CAGCAGCTGACCCGGGCCGGGCACAGCGTCACCGTCTTCGAGCGGTCCGACAAGATCGGCGGGCTGCTGCGCTACGGCAT
CCCGGAATTCAAGATGGAAAAGCGGGTCCTGGACCGCCGGCTGGACCAGATGCGCGCCGAGGGAACCGAATTCCGCGCCG
GCGTCAACGTCGGTGTCGACATCACCGCGGACCAGCTGCGCGCCGACTTCGACGCCGTCGTGCTGGCCGGCGGCGCCACC
GCCTGGCGGGATCTGCCCATTCCCGGCCGGGAGCTGGACGGCATCCACCAGGCGATGGAGTTCTTGCCGTGGGGTAACCG
GCAAGCCTCGGGCGACCTGGACGAGGCCGACATGGACGCCGACGGGCAGCCGCCGATCACCGCCAAGGGCAAGAAGGTCA
TCATCATCGGCGGCGGCGACACCGGCGCCGACTGCCTGGGCACCGTGCACCGGCAGGGCGCCACCGAGGTGCACCAGTTC
GAGATCATGCCGCGTCCGCCGGACACCCGCGCCCCGTCCACCCCGTGGCCGACCTACCCGCTGATGTACCGGGTGTCCTC
GGCGCACGAGGAGGGCGGCGAGCGGGTGTTCTCGGTCAACACCGAGGAGTTCGTCGGCGAGAACGGTCGCGTCACGGCGC
TCAAGGCGCACGAGGTGACCATGGAGGACGGCAAGTTCGTCAAGGTCGAGGGCTCGGAGTTCGAACTCGAGGCCGACCTG
GTGCTGCTGGCGATGGGATTCGTCGGCCCCGAGAAGGAGGGCCTGCTCACCGACCTCGGGGTGAAGTTCACCGAGCGCGG
CAACGTCGCGCGCGGCGCCGATTTCGACACCTCGATTCCGGGCGTGTTCGTGGCCGGGGACATGGGTCGCGGCCAGTCGT
TGATCGTCTGGGCGATAGCTGAGGGCCGGGCGGCCGCGGCGGCGGCGGATCGGTTCCTGATGGGCGCCACCGCGCTGCCG
GCGCCGGTCAAACCCACCGCGGTACCGCTGCAGTAG

Upstream 100 bases:

>100_bases
GTCAAGGTGATGCCGCGCGACTACAAGCGGGTGCTGCAGGCCATCGCCGAAGCCGAACGTGACGGCGGCGATGTCGACAA
GGCGATCATGGCGGCCGCGC

Downstream 100 bases:

>100_bases
TAACACCAGTCACACCAGAAACACGACCGGTTTCGCTCGGCGCGTCTTCCACAGTTTGCCAGGACGTTGGTGTCTAATAA
CCCGTTGTGAGCCCGCTCAC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MADPSGFLKYTHRELPKRRPVPLRLRDWREVYEEFDHGTLREQATRCMDCGIPFCHNGCPLGNLIPEWNDLVRTGRWRDA
IERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQIELEIIDNAFDEGWVQPLPPDKLTGKKVAVVGSGPAGLAAA
QQLTRAGHSVTVFERSDKIGGLLRYGIPEFKMEKRVLDRRLDQMRAEGTEFRAGVNVGVDITADQLRADFDAVVLAGGAT
AWRDLPIPGRELDGIHQAMEFLPWGNRQASGDLDEADMDADGQPPITAKGKKVIIIGGGDTGADCLGTVHRQGATEVHQF
EIMPRPPDTRAPSTPWPTYPLMYRVSSAHEEGGERVFSVNTEEFVGENGRVTALKAHEVTMEDGKFVKVEGSEFELEADL
VLLAMGFVGPEKEGLLTDLGVKFTERGNVARGADFDTSIPGVFVAGDMGRGQSLIVWAIAEGRAAAAAADRFLMGATALP
APVKPTAVPLQ

Sequences:

>Translated_491_residues
MADPSGFLKYTHRELPKRRPVPLRLRDWREVYEEFDHGTLREQATRCMDCGIPFCHNGCPLGNLIPEWNDLVRTGRWRDA
IERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQIELEIIDNAFDEGWVQPLPPDKLTGKKVAVVGSGPAGLAAA
QQLTRAGHSVTVFERSDKIGGLLRYGIPEFKMEKRVLDRRLDQMRAEGTEFRAGVNVGVDITADQLRADFDAVVLAGGAT
AWRDLPIPGRELDGIHQAMEFLPWGNRQASGDLDEADMDADGQPPITAKGKKVIIIGGGDTGADCLGTVHRQGATEVHQF
EIMPRPPDTRAPSTPWPTYPLMYRVSSAHEEGGERVFSVNTEEFVGENGRVTALKAHEVTMEDGKFVKVEGSEFELEADL
VLLAMGFVGPEKEGLLTDLGVKFTERGNVARGADFDTSIPGVFVAGDMGRGQSLIVWAIAEGRAAAAAADRFLMGATALP
APVKPTAVPLQ
>Mature_490_residues
ADPSGFLKYTHRELPKRRPVPLRLRDWREVYEEFDHGTLREQATRCMDCGIPFCHNGCPLGNLIPEWNDLVRTGRWRDAI
ERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQIELEIIDNAFDEGWVQPLPPDKLTGKKVAVVGSGPAGLAAAQ
QLTRAGHSVTVFERSDKIGGLLRYGIPEFKMEKRVLDRRLDQMRAEGTEFRAGVNVGVDITADQLRADFDAVVLAGGATA
WRDLPIPGRELDGIHQAMEFLPWGNRQASGDLDEADMDADGQPPITAKGKKVIIIGGGDTGADCLGTVHRQGATEVHQFE
IMPRPPDTRAPSTPWPTYPLMYRVSSAHEEGGERVFSVNTEEFVGENGRVTALKAHEVTMEDGKFVKVEGSEFELEADLV
LLAMGFVGPEKEGLLTDLGVKFTERGNVARGADFDTSIPGVFVAGDMGRGQSLIVWAIAEGRAAAAAADRFLMGATALPA
PVKPTAVPLQ

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=488, Percent_Identity=24.5901639344262, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI111118983, Length=161, Percent_Identity=32.9192546583851, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI111118981, Length=161, Percent_Identity=32.9192546583851, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=38.4615384615385, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1788811, Length=465, Percent_Identity=35.0537634408602, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=34.9344978165939, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788468, Length=439, Percent_Identity=26.4236902050114, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789243, Length=429, Percent_Identity=27.039627039627, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=497, Percent_Identity=46.6800804828974, Blast_Score=443, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17543860, Length=175, Percent_Identity=36.5714285714286, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71984108, Length=464, Percent_Identity=22.4137931034483, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=495, Percent_Identity=47.6767676767677, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320584, Length=179, Percent_Identity=30.7262569832402, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574881, Length=495, Percent_Identity=51.5151515151515, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=495, Percent_Identity=51.5151515151515, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=495, Percent_Identity=51.5151515151515, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665543, Length=495, Percent_Identity=51.5151515151515, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640763, Length=479, Percent_Identity=25.2609603340292, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18858217, Length=479, Percent_Identity=25.2609603340292, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53507; Mature: 53376

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPSGFLKYTHRELPKRRPVPLRLRDWREVYEEFDHGTLREQATRCMDCGIPFCHNGCP
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
LGNLIPEWNDLVRTGRWRDAIERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQI
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
ELEIIDNAFDEGWVQPLPPDKLTGKKVAVVGSGPAGLAAAQQLTRAGHSVTVFERSDKIG
EEEHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GLLRYGIPEFKMEKRVLDRRLDQMRAEGTEFRAGVNVGVDITADQLRADFDAVVLAGGAT
CHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHCCCCEEEEECCCC
AWRDLPIPGRELDGIHQAMEFLPWGNRQASGDLDEADMDADGQPPITAKGKKVIIIGGGD
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
TGADCLGTVHRQGATEVHQFEIMPRPPDTRAPSTPWPTYPLMYRVSSAHEEGGERVFSVN
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHEEEEHHHHHCCCCEEEEEC
TEEFVGENGRVTALKAHEVTMEDGKFVKVEGSEFELEADLVLLAMGFVGPEKEGLLTDLG
HHHHHCCCCCEEEEEEEEEEECCCCEEEEECCCEEEECCEEEEEHHCCCCCCCCCCHHCC
VKFTERGNVARGADFDTSIPGVFVAGDMGRGQSLIVWAIAEGRAAAAAADRFLMGATALP
CEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCC
APVKPTAVPLQ
CCCCCCCCCCC
>Mature Secondary Structure 
ADPSGFLKYTHRELPKRRPVPLRLRDWREVYEEFDHGTLREQATRCMDCGIPFCHNGCP
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
LGNLIPEWNDLVRTGRWRDAIERLHATNNFPDFTGRLCPAPCEPACVLGINQDPVTIKQI
CCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEE
ELEIIDNAFDEGWVQPLPPDKLTGKKVAVVGSGPAGLAAAQQLTRAGHSVTVFERSDKIG
EEEHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GLLRYGIPEFKMEKRVLDRRLDQMRAEGTEFRAGVNVGVDITADQLRADFDAVVLAGGAT
CHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHCCCCEEEEECCCC
AWRDLPIPGRELDGIHQAMEFLPWGNRQASGDLDEADMDADGQPPITAKGKKVIIIGGGD
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
TGADCLGTVHRQGATEVHQFEIMPRPPDTRAPSTPWPTYPLMYRVSSAHEEGGERVFSVN
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHEEEEHHHHHCCCCEEEEEC
TEEFVGENGRVTALKAHEVTMEDGKFVKVEGSEFELEADLVLLAMGFVGPEKEGLLTDLG
HHHHHCCCCCEEEEEEEEEEECCCCEEEEECCCEEEECCEEEEEHHCCCCCCCCCCHHCC
VKFTERGNVARGADFDTSIPGVFVAGDMGRGQSLIVWAIAEGRAAAAAADRFLMGATALP
CEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCC
APVKPTAVPLQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]