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The map label for this gene is pqqE [H]

Identifier: 39998543

GI number: 39998543

Start: 3801376

End: 3802446

Strand: Reverse

Name: pqqE [H]

Synonym: GSU3454

Alternate gene names: 39998543

Gene position: 3802446-3801376 (Counterclockwise)

Preceding gene: 308513345

Following gene: 39998542

Centisome position: 99.69

GC content: 57.8

Gene sequence:

>1071_bases
ATGGCCGAAGAATTCATTCCCAAGTGGGTTGCCTGGGAAACCACCCAGAAATGCAACCTCAAGTGCGTCCACTGTCGCTG
TTCCTCTGACCTGACCTCCTCCGAAGGTGATTTCACCACCGAGGAAGGGAAGAAGCTCCTGAAGGAGATTGCCGATTTCT
CAAAACCGGTCATCGTTCTTTCGGGCGGTGAGCCCCTCATGCGCCAGGATATCTTTGAGTTGGCCGAGTACGGCACCTCT
CTGGGGCTGCGGATGTGCATGGCCACCAACGGCGCCTTGGTCACCGACGAAATCTGCGCCAAAATGAGGAAGGCGGACAT
CAAGATGGTGTCCCTCTCTCTGGATGGCAGCAGCGCCGAGGTCCACGACGATTTCCGCCAGTGTCCCGGTGCCTTCGAGG
GAGTGGTGCGGGCCGCCGAAATCTTCCGGCGTAACGGCCAGAAATTCCTCATAAATTCTTCCTTTACCAAACGGAACCAG
CACGATATCGCCAACACTTTCAAGCTGGCCAAGAGCCTGGGCGCCACTGCCTGGTACATGTTCATGATCGTCCCCACGGG
CCGGGGCGAGGAGATCATGAACGAGCTCATCTCCAAGGAGGACTACGAGGAGATCCTGGAGTGGCACTACCAGCAGGAGA
AGCTTGAGGACGAAATTCTCATGCGCCCCACCTGTGCCCCCCACTACTACCGGATGGTCCCCATGCGGGCCAAGCAGGAA
GGGGTCAAGTTCGAACGCCGCTCCCTGACCTTCTCCACCGGCGGCGGCAAAGGATGCATCGCGGCCCAGACCATCTGTCT
CATCGACTGCTTCGGCAACGTAAAGCCCTGCTCCTACTTCCACCGTACTGCCGGCAACGTGAAGGAAATCCCCTTCCGGG
AAATCTGGGAGAACTCGGAAATCTTCAAGGATCTCCGTAACTTCAAGGCATACAAGGGGAAATGCGGCGAGTGTGAGTTC
ATCAACGTCTGCGGCGGCTGCCGGGCCCGGGCCGACGCGGTCTATGGGGATTACATGGAAGAGGAGCCGTTCTGTAATTA
CGTGCCGATACGCCTTCAGAAGAAGGCGTAA

Upstream 100 bases:

>100_bases
AAAGATGGCAGAGTAGTACCGGCCCCCGGCCGCTAAACTTCTTGTGCTGTCGAGGTTATTTTAGTAGGATTTACAGGATT
GTCACACGAACGGAGAATCC

Downstream 100 bases:

>100_bases
GGAACGCGATAGTTACGCCGTGTTGGCGTCGTCCTCGTCACCCCCTTGTGCGGCGTAGCCGGGCTACGCCTCCGCCGGGG
TTACTGCGGGTGCAGCAATT

Product: radical SAM protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAEEFIPKWVAWETTQKCNLKCVHCRCSSDLTSSEGDFTTEEGKKLLKEIADFSKPVIVLSGGEPLMRQDIFELAEYGTS
LGLRMCMATNGALVTDEICAKMRKADIKMVSLSLDGSSAEVHDDFRQCPGAFEGVVRAAEIFRRNGQKFLINSSFTKRNQ
HDIANTFKLAKSLGATAWYMFMIVPTGRGEEIMNELISKEDYEEILEWHYQQEKLEDEILMRPTCAPHYYRMVPMRAKQE
GVKFERRSLTFSTGGGKGCIAAQTICLIDCFGNVKPCSYFHRTAGNVKEIPFREIWENSEIFKDLRNFKAYKGKCGECEF
INVCGGCRARADAVYGDYMEEEPFCNYVPIRLQKKA

Sequences:

>Translated_356_residues
MAEEFIPKWVAWETTQKCNLKCVHCRCSSDLTSSEGDFTTEEGKKLLKEIADFSKPVIVLSGGEPLMRQDIFELAEYGTS
LGLRMCMATNGALVTDEICAKMRKADIKMVSLSLDGSSAEVHDDFRQCPGAFEGVVRAAEIFRRNGQKFLINSSFTKRNQ
HDIANTFKLAKSLGATAWYMFMIVPTGRGEEIMNELISKEDYEEILEWHYQQEKLEDEILMRPTCAPHYYRMVPMRAKQE
GVKFERRSLTFSTGGGKGCIAAQTICLIDCFGNVKPCSYFHRTAGNVKEIPFREIWENSEIFKDLRNFKAYKGKCGECEF
INVCGGCRARADAVYGDYMEEEPFCNYVPIRLQKKA
>Mature_355_residues
AEEFIPKWVAWETTQKCNLKCVHCRCSSDLTSSEGDFTTEEGKKLLKEIADFSKPVIVLSGGEPLMRQDIFELAEYGTSL
GLRMCMATNGALVTDEICAKMRKADIKMVSLSLDGSSAEVHDDFRQCPGAFEGVVRAAEIFRRNGQKFLINSSFTKRNQH
DIANTFKLAKSLGATAWYMFMIVPTGRGEEIMNELISKEDYEEILEWHYQQEKLEDEILMRPTCAPHYYRMVPMRAKQEG
VKFERRSLTFSTGGGKGCIAAQTICLIDCFGNVKPCSYFHRTAGNVKEIPFREIWENSEIFKDLRNFKAYKGKCGECEFI
NVCGGCRARADAVYGDYMEEEPFCNYVPIRLQKKA

Specific function: Unknown

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40483; Mature: 40352

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEFIPKWVAWETTQKCNLKCVHCRCSSDLTSSEGDFTTEEGKKLLKEIADFSKPVIVL
CCCHHCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
SGGEPLMRQDIFELAEYGTSLGLRMCMATNGALVTDEICAKMRKADIKMVSLSLDGSSAE
ECCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEHHHHHHHHHHCCEEEEEEEECCCCCH
VHDDFRQCPGAFEGVVRAAEIFRRNGQKFLINSSFTKRNQHDIANTFKLAKSLGATAWYM
HHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
FMIVPTGRGEEIMNELISKEDYEEILEWHYQQEKLEDEILMRPTCAPHYYRMVPMRAKQE
EEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHC
GVKFERRSLTFSTGGGKGCIAAQTICLIDCFGNVKPCSYFHRTAGNVKEIPFREIWENSE
CCCEEHHEEEEECCCCCCCHHHHHHHEEECCCCCCCHHHHHHCCCCCCCCCHHHHHCCHH
IFKDLRNFKAYKGKCGECEFINVCGGCRARADAVYGDYMEEEPFCNYVPIRLQKKA
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
AEEFIPKWVAWETTQKCNLKCVHCRCSSDLTSSEGDFTTEEGKKLLKEIADFSKPVIVL
CCHHCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
SGGEPLMRQDIFELAEYGTSLGLRMCMATNGALVTDEICAKMRKADIKMVSLSLDGSSAE
ECCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEHHHHHHHHHHCCEEEEEEEECCCCCH
VHDDFRQCPGAFEGVVRAAEIFRRNGQKFLINSSFTKRNQHDIANTFKLAKSLGATAWYM
HHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
FMIVPTGRGEEIMNELISKEDYEEILEWHYQQEKLEDEILMRPTCAPHYYRMVPMRAKQE
EEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHC
GVKFERRSLTFSTGGGKGCIAAQTICLIDCFGNVKPCSYFHRTAGNVKEIPFREIWENSE
CCCEEHHEEEEECCCCCCCHHHHHHHEEECCCCCCCHHHHHHCCCCCCCCCHHHHHCCHH
IFKDLRNFKAYKGKCGECEFINVCGGCRARADAVYGDYMEEEPFCNYVPIRLQKKA
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]