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The map label for this gene is nuoE-2 [H]

Identifier: 39998532

GI number: 39998532

Start: 3788267

End: 3788755

Strand: Reverse

Name: nuoE-2 [H]

Synonym: GSU3443

Alternate gene names: 39998532

Gene position: 3788755-3788267 (Counterclockwise)

Preceding gene: 39998533

Following gene: 39998531

Centisome position: 99.33

GC content: 67.89

Gene sequence:

>489_bases
ATGATCTCGGAGCTTCTCAAAAAGGAACTTCAGGCCCGCGTCGCTCACGCCGTCACCAGCCGTGAGGCGGCCGTGGACGT
GATGAAAGCGCTGCAGCGCCACTACGGCTGGCTCACCGACGAGGCAGTGGGGGAGGCGGCGGAGCTCCTGGGACTCACCC
CCCTCCAGGTGGAGGAGCTGGCCACCTTCTACGAGATGATCTACCGCCGCCCCGTGGGAAAGCGGGTGATCCACGTCTGC
GACTCCATTTCTTGCTGGGCCCTGGGGGGCGAGAGCCTCATGGCGCACCTGGCAGCGGCGCTGAACATCGAACCGGGGGG
AACCACGGCGGACGGGCTCTTTACGCTTCTGCCCTGCTGCTGCCTTGGCAACTGCGGCGAAGCCCCGACGCTCATGGTCG
GCGACACCCTCCACGGCCGGGTGACGCTGGAGCGGGCCGGGGAGATCCTGGCGACTGAGCGGCGGCAGCTTGCAGCCGCG
GAGGGATGA

Upstream 100 bases:

>100_bases
AGGCCCTCCCCCTCATGAGCCGGGGGTGGCTCGTGGCGGATTTTCTGGCCATCATCGGGTCCATTGACTTCGTGCTGGCG
GATTTGGACAGGTGACGCCG

Downstream 100 bases:

>100_bases
ACGATGGTGCGAGTAAAGCGGATCTACGACGAACCGGCAACCGAGGACGGCACGCGCGTCCTGGTGGACCGGCTCTGGCC
CCGGGGTATCGCGAAAGACA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MISELLKKELQARVAHAVTSREAAVDVMKALQRHYGWLTDEAVGEAAELLGLTPLQVEELATFYEMIYRRPVGKRVIHVC
DSISCWALGGESLMAHLAAALNIEPGGTTADGLFTLLPCCCLGNCGEAPTLMVGDTLHGRVTLERAGEILATERRQLAAA
EG

Sequences:

>Translated_162_residues
MISELLKKELQARVAHAVTSREAAVDVMKALQRHYGWLTDEAVGEAAELLGLTPLQVEELATFYEMIYRRPVGKRVIHVC
DSISCWALGGESLMAHLAAALNIEPGGTTADGLFTLLPCCCLGNCGEAPTLMVGDTLHGRVTLERAGEILATERRQLAAA
EG
>Mature_162_residues
MISELLKKELQARVAHAVTSREAAVDVMKALQRHYGWLTDEAVGEAAELLGLTPLQVEELATFYEMIYRRPVGKRVIHVC
DSISCWALGGESLMAHLAAALNIEPGGTTADGLFTLLPCCCLGNCGEAPTLMVGDTLHGRVTLERAGEILATERRQLAAA
EG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=145, Percent_Identity=36.551724137931, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788621, Length=144, Percent_Identity=40.9722222222222, Blast_Score=131, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17561328, Length=128, Percent_Identity=37.5, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18859877, Length=131, Percent_Identity=36.6412213740458, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21355353, Length=123, Percent_Identity=38.2113821138211, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17416; Mature: 17416

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISELLKKELQARVAHAVTSREAAVDVMKALQRHYGWLTDEAVGEAAELLGLTPLQVEEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHH
ATFYEMIYRRPVGKRVIHVCDSISCWALGGESLMAHLAAALNIEPGGTTADGLFTLLPCC
HHHHHHHHHCCHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
CLGNCGEAPTLMVGDTLHGRVTLERAGEILATERRQLAAAEG
HCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISELLKKELQARVAHAVTSREAAVDVMKALQRHYGWLTDEAVGEAAELLGLTPLQVEEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHH
ATFYEMIYRRPVGKRVIHVCDSISCWALGGESLMAHLAAALNIEPGGTTADGLFTLLPCC
HHHHHHHHHCCHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
CLGNCGEAPTLMVGDTLHGRVTLERAGEILATERRQLAAAEG
HCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]