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The map label for this gene is nuoI-2 [H]

Identifier: 39998523

GI number: 39998523

Start: 3779688

End: 3780218

Strand: Reverse

Name: nuoI-2 [H]

Synonym: GSU3434

Alternate gene names: 39998523

Gene position: 3780218-3779688 (Counterclockwise)

Preceding gene: 39998524

Following gene: 39998522

Centisome position: 99.11

GC content: 66.1

Gene sequence:

>531_bases
ATGCCGATACTGACCGATTTCAAAGCCATCGCCACCGGGCTTTTCGTGACCTGGAAGCATATCTTCCGCAGGCCGGTGAC
CGTGGAGTACCCGGAGGTGAAGCGGACGCCGGCGCCCCGCTACCGGGCGCGGATCGTTCTCACCCGCGACCCGGACGGCG
GAGAGCGCTGCGTGGCCTGCTACCTCTGCTCCGCGGCCTGTCCCGTTGACTGCATCTCCATGGAAGCGGCCGAAGGGGAG
GAGGGCCGCCGCTACGCCCGGTGGTTCCGGATCAACTTCTCCCGCTGCATCTTCTGCGGCCTCTGCGCCGAGGCCTGTCC
GACCCTGGCCATCCAGATGACCCCCGACTACGAGATCTGCGAACGGGACATCATGGAACTGGTCTACGAGAAGGAGGACC
TCCTCATCGATGGCTGCGGCAAGGATGCCGGGTACAACTTCTACCGTCACGCCGGCATCGGCGTCGCCCAGCCCCGCGGG
GCTGGCGAATGCGAGGAAGAGCCGGTGGACGTGCGGGGACTGATGCCCTAA

Upstream 100 bases:

>100_bases
ACCTGATCAAAAAGGCGCAACGGGCCAAGTCGGGCACCGCCTGACCGCCGCGTGAACCGGTGGAGGGGCTCTGGCCGGAG
CCCCTCCACTCCCGTGAACC

Downstream 100 bases:

>100_bases
CGGTATGGAACAGGCTCTTTTCTACATCCTTGCCGCCGTGACCGTCATCGCCACGGTTCTTGCCATCACCGAGAAGCACG
CGGTGCACGCCATCGTCTAC

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 1; NDH-1 subunit I 1 [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPILTDFKAIATGLFVTWKHIFRRPVTVEYPEVKRTPAPRYRARIVLTRDPDGGERCVACYLCSAACPVDCISMEAAEGE
EGRRYARWFRINFSRCIFCGLCAEACPTLAIQMTPDYEICERDIMELVYEKEDLLIDGCGKDAGYNFYRHAGIGVAQPRG
AGECEEEPVDVRGLMP

Sequences:

>Translated_176_residues
MPILTDFKAIATGLFVTWKHIFRRPVTVEYPEVKRTPAPRYRARIVLTRDPDGGERCVACYLCSAACPVDCISMEAAEGE
EGRRYARWFRINFSRCIFCGLCAEACPTLAIQMTPDYEICERDIMELVYEKEDLLIDGCGKDAGYNFYRHAGIGVAQPRG
AGECEEEPVDVRGLMP
>Mature_175_residues
PILTDFKAIATGLFVTWKHIFRRPVTVEYPEVKRTPAPRYRARIVLTRDPDGGERCVACYLCSAACPVDCISMEAAEGEE
GRRYARWFRINFSRCIFCGLCAEACPTLAIQMTPDYEICERDIMELVYEKEDLLIDGCGKDAGYNFYRHAGIGVAQPRGA
GECEEEPVDVRGLMP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=126, Percent_Identity=42.8571428571429, Blast_Score=110, Evalue=7e-25,
Organism=Escherichia coli, GI1788617, Length=175, Percent_Identity=55.4285714285714, Blast_Score=191, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17555194, Length=132, Percent_Identity=40.9090909090909, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17864306, Length=132, Percent_Identity=43.1818181818182, Blast_Score=116, Evalue=7e-27,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19843; Mature: 19712

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
6.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILTDFKAIATGLFVTWKHIFRRPVTVEYPEVKRTPAPRYRARIVLTRDPDGGERCVAC
CCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHE
YLCSAACPVDCISMEAAEGEEGRRYARWFRINFSRCIFCGLCAEACPTLAIQMTPDYEIC
EHHCCCCCCHHEECCCCCCCCCHHEEHEEEECHHHHHHHHHHHHCCCEEEEEECCCHHHH
ERDIMELVYEKEDLLIDGCGKDAGYNFYRHAGIGVAQPRGAGECEEEPVDVRGLMP
HHHHHHHHHCCCCEEEECCCCCCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PILTDFKAIATGLFVTWKHIFRRPVTVEYPEVKRTPAPRYRARIVLTRDPDGGERCVAC
CCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHE
YLCSAACPVDCISMEAAEGEEGRRYARWFRINFSRCIFCGLCAEACPTLAIQMTPDYEIC
EHHCCCCCCHHEECCCCCCCCCHHEEHEEEECHHHHHHHHHHHHCCCEEEEEECCCHHHH
ERDIMELVYEKEDLLIDGCGKDAGYNFYRHAGIGVAQPRGAGECEEEPVDVRGLMP
HHHHHHHHHCCCCEEEECCCCCCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA