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The map label for this gene is braC [H]

Identifier: 39998490

GI number: 39998490

Start: 3743342

End: 3744532

Strand: Reverse

Name: braC [H]

Synonym: GSU3401

Alternate gene names: 39998490

Gene position: 3744532-3743342 (Counterclockwise)

Preceding gene: 39998491

Following gene: 39998484

Centisome position: 98.18

GC content: 61.13

Gene sequence:

>1191_bases
ATGAAATTCAGGAAAATTGCATCGCTGCTGACCGTTGCCGCAGTAGCCTCGGCCTTTGCGGTGGGCTTTGGCTGCAAGAA
GAAGGAAGAAGCTCCGGCCGGCGCCCCGGCTGCCGCCGGCGACACCATCAAGATCGGCTTCCTCGGCGCCCTGACCGGCG
ACGTGGCCATGTTCGGCAAGCCGACCCTCGACGGCATGAAGATGGCCGCTGAAGAACTCAACGCAGCAGGCGGCGTTCTC
GGCAAGAAGATCGAGATCGTCGAGGCCGATAACCGTGGCGACAAGCAGGAAGGTGCCTCGGTCACCCAAAAGCTCATCAG
CCGTGACGGCGTAGTTGCCATCGTTGGCGACCCCACCACCGGCATTACCAAGGTTGCCGCTCCCATTGCCCAGAAGGCCC
AGGTGGTACTCCTCTCCGCCGGCGCCACGGGCCCCGGCGTCGTGGAAAACGGCGATTTCATCTTCCGCAACACCCTGCTG
GACAGCGTCGCCATCCCGGCCTGCATCGACTTCTTCGCCAAGGACCTGGGCTACAAGAAGGTTGCGATCATCACCTCCGA
CAACAACGATTACAGCGTGGGCCTCTCCCAGACCTTCCGCGACGCCGCCAAGGGCAAGGGTGTTGAAATCGTTGCCGACG
AGAAGGTAAAGGACGGCGACAAGGACTTCAGCGCCCAGATCACCAACATCAAGGGCAAGAAGCCCGATGTTATCTTCTTC
TCCGGCTACTACACCGAGGGTGCCCTCATCATGAAGGAAGCCCGCAAGCAGGGCCTCAAGGCCAAGATGTTCGGTGGCGA
CGGCCTCTTCTCGCCGAAGCTGATCGAGCTGGGCGGCGATGCGGTTGAGGGCACCATGTCCGCCCTCGGCTTCTCTCCCG
AGCAGGCTTCTCCGGTTACCGCCAAGTTCGTCGAGGCGTACAAGAAGAAGTTCAACGGCGTCGAACCGGGTCTCTTCGAC
GCTCAGGGATATGACGGCGTCATGATGCTGGCCGATGCCATGAAGCGTGCCAACAGCGCAGATCCGAAGGTGTTCAAGAC
CGCCCTCGGCCAGACCAAGAACTATGAAGGGGTCTCGGGGACCATCACCATCCGCGAGAACCGCGAGCCGATCAAGTCGC
CGCTGGCCCTTCTGGAAGTAAAGGGTGGGAAGTTCGCCCTGAAGGCCAAGGTTCCGGTCAAGATGGACTAA

Upstream 100 bases:

>100_bases
CTCAAAGAGGCCAACGAGATGGTCGACGTGCTGCTGAAGCAAGGCGGCGGCGGCAAGTAATTCCCCCTGCTGCACCATTC
ATTCAACAGGAGGAGCACCC

Downstream 100 bases:

>100_bases
GAACCGCCGGCATAGCAAAGAACAAAAGCCCCACGGGAGCAATCCCGTGGGGCTTTTGTTTGGCTGCGGCAGAGCCGGCC
CGGCACAAGCTCGCCCCCTT

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKFRKIASLLTVAAVASAFAVGFGCKKKEEAPAGAPAAAGDTIKIGFLGALTGDVAMFGKPTLDGMKMAAEELNAAGGVL
GKKIEIVEADNRGDKQEGASVTQKLISRDGVVAIVGDPTTGITKVAAPIAQKAQVVLLSAGATGPGVVENGDFIFRNTLL
DSVAIPACIDFFAKDLGYKKVAIITSDNNDYSVGLSQTFRDAAKGKGVEIVADEKVKDGDKDFSAQITNIKGKKPDVIFF
SGYYTEGALIMKEARKQGLKAKMFGGDGLFSPKLIELGGDAVEGTMSALGFSPEQASPVTAKFVEAYKKKFNGVEPGLFD
AQGYDGVMMLADAMKRANSADPKVFKTALGQTKNYEGVSGTITIRENREPIKSPLALLEVKGGKFALKAKVPVKMD

Sequences:

>Translated_396_residues
MKFRKIASLLTVAAVASAFAVGFGCKKKEEAPAGAPAAAGDTIKIGFLGALTGDVAMFGKPTLDGMKMAAEELNAAGGVL
GKKIEIVEADNRGDKQEGASVTQKLISRDGVVAIVGDPTTGITKVAAPIAQKAQVVLLSAGATGPGVVENGDFIFRNTLL
DSVAIPACIDFFAKDLGYKKVAIITSDNNDYSVGLSQTFRDAAKGKGVEIVADEKVKDGDKDFSAQITNIKGKKPDVIFF
SGYYTEGALIMKEARKQGLKAKMFGGDGLFSPKLIELGGDAVEGTMSALGFSPEQASPVTAKFVEAYKKKFNGVEPGLFD
AQGYDGVMMLADAMKRANSADPKVFKTALGQTKNYEGVSGTITIRENREPIKSPLALLEVKGGKFALKAKVPVKMD
>Mature_396_residues
MKFRKIASLLTVAAVASAFAVGFGCKKKEEAPAGAPAAAGDTIKIGFLGALTGDVAMFGKPTLDGMKMAAEELNAAGGVL
GKKIEIVEADNRGDKQEGASVTQKLISRDGVVAIVGDPTTGITKVAAPIAQKAQVVLLSAGATGPGVVENGDFIFRNTLL
DSVAIPACIDFFAKDLGYKKVAIITSDNNDYSVGLSQTFRDAAKGKGVEIVADEKVKDGDKDFSAQITNIKGKKPDVIFF
SGYYTEGALIMKEARKQGLKAKMFGGDGLFSPKLIELGGDAVEGTMSALGFSPEQASPVTAKFVEAYKKKFNGVEPGLFD
AQGYDGVMMLADAMKRANSADPKVFKTALGQTKNYEGVSGTITIRENREPIKSPLALLEVKGGKFALKAKVPVKMD

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=307, Percent_Identity=29.9674267100977, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI48994941, Length=274, Percent_Identity=28.1021897810219, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41480; Mature: 41480

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFRKIASLLTVAAVASAFAVGFGCKKKEEAPAGAPAAAGDTIKIGFLGALTGDVAMFGK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCCC
PTLDGMKMAAEELNAAGGVLGKKIEIVEADNRGDKQEGASVTQKLISRDGVVAIVGDPTT
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
GITKVAAPIAQKAQVVLLSAGATGPGVVENGDFIFRNTLLDSVAIPACIDFFAKDLGYKK
CHHHHHHHHHCCCEEEEEECCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCCEE
VAIITSDNNDYSVGLSQTFRDAAKGKGVEIVADEKVKDGDKDFSAQITNIKGKKPDVIFF
EEEEEECCCCEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCEEEE
SGYYTEGALIMKEARKQGLKAKMFGGDGLFSPKLIELGGDAVEGTMSALGFSPEQASPVT
ECEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCHH
AKFVEAYKKKFNGVEPGLFDAQGYDGVMMLADAMKRANSADPKVFKTALGQTKNYEGVSG
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEE
TITIRENREPIKSPLALLEVKGGKFALKAKVPVKMD
EEEEECCCCCHHCCEEEEEECCCEEEEEEECCEECC
>Mature Secondary Structure
MKFRKIASLLTVAAVASAFAVGFGCKKKEEAPAGAPAAAGDTIKIGFLGALTGDVAMFGK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCCC
PTLDGMKMAAEELNAAGGVLGKKIEIVEADNRGDKQEGASVTQKLISRDGVVAIVGDPTT
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
GITKVAAPIAQKAQVVLLSAGATGPGVVENGDFIFRNTLLDSVAIPACIDFFAKDLGYKK
CHHHHHHHHHCCCEEEEEECCCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCCEE
VAIITSDNNDYSVGLSQTFRDAAKGKGVEIVADEKVKDGDKDFSAQITNIKGKKPDVIFF
EEEEEECCCCEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCEEEE
SGYYTEGALIMKEARKQGLKAKMFGGDGLFSPKLIELGGDAVEGTMSALGFSPEQASPVT
ECEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCCHH
AKFVEAYKKKFNGVEPGLFDAQGYDGVMMLADAMKRANSADPKVFKTALGQTKNYEGVSG
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEE
TITIRENREPIKSPLALLEVKGGKFALKAKVPVKMD
EEEEECCCCCHHCCEEEEEECCCEEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]