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The map label for this gene is lutR [H]

Identifier: 39998485

GI number: 39998485

Start: 3735764

End: 3736480

Strand: Direct

Name: lutR [H]

Synonym: GSU3396

Alternate gene names: 39998485

Gene position: 3735764-3736480 (Clockwise)

Preceding gene: 39998477

Following gene: 39998486

Centisome position: 97.95

GC content: 62.2

Gene sequence:

>717_bases
ATGTTTCGCAAGGCCAAGCAAAACCGGATCTTTCAAGACGTGGTGGATCAGATACAGGAAGCGATCGTGGAAGGGAGGCT
CAAGGAGGGGGACCAGCTTCCGGCAGAGCGAAAGCTCCAGGAAGAGTTCAATGTAAGCAGGGGTACCCTCCGGGAGGCGC
TCCGCGTCCTGGAACAGAAGGGGCTCATCGAGATCCGGACCGGCGTGGCCGGGGGATCGGTGATCAAGGGGGTATCCACC
GAGCAGGTGAGCGAGAGCCTGGCGCTGCTCATCCGCTACCAGAAGGTGCCACTGGCGAAGCTGGCCGAATTCCGCGAGGG
CGTCGAAGGCATCGTGGCCGCGTTGGCCGCAAACCGGGCAACCTCCGAGCAGACAAGCCGGCTGCAGGAGCTGTTGAGCA
CGGCCGGTGAGTACCTGGAGCAGGGGGAGCCCCACTGGGATGCCTTCATCCGGACCGACGAGGAGTTTCACCTGGCCCTG
GCCGAGATGACCGACAACCCTCTCTTCATCACGGTGCTCGAAACCGTCTATCACAACATCCATACCTACTACGAGCGCTA
CCTGCCCATGGAGGAAACGGTGCTGCGCCAGAACTACCAGGACTTGAAGGATTTGTTCGCGGCTGTCGCCGCCCGGGACG
GGAAACGGGCCGAAGAGTTGGCCAGGGAGCACGTGCGGCGCTTCAACACGCGGATGGAGGAGCGCCATATCTCCTGA

Upstream 100 bases:

>100_bases
CGGGCCGACGACGTAGACCGCGCCGGCTGTTCAACAAGCCGGCATGGGTATAACCATTGTAATCCTGCATTGCTACGGTG
CAACGGAAGGAGCAACCCAG

Downstream 100 bases:

>100_bases
CCGAGAGACTTCGCACAGGGTTGTTGACAGCCATCCTTCCATTTTTTTATAGTTCTATCTACATTTACCTGCACGAAAGT
GTTCTCAAGTGTTCTCCATG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MFRKAKQNRIFQDVVDQIQEAIVEGRLKEGDQLPAERKLQEEFNVSRGTLREALRVLEQKGLIEIRTGVAGGSVIKGVST
EQVSESLALLIRYQKVPLAKLAEFREGVEGIVAALAANRATSEQTSRLQELLSTAGEYLEQGEPHWDAFIRTDEEFHLAL
AEMTDNPLFITVLETVYHNIHTYYERYLPMEETVLRQNYQDLKDLFAAVAARDGKRAEELAREHVRRFNTRMEERHIS

Sequences:

>Translated_238_residues
MFRKAKQNRIFQDVVDQIQEAIVEGRLKEGDQLPAERKLQEEFNVSRGTLREALRVLEQKGLIEIRTGVAGGSVIKGVST
EQVSESLALLIRYQKVPLAKLAEFREGVEGIVAALAANRATSEQTSRLQELLSTAGEYLEQGEPHWDAFIRTDEEFHLAL
AEMTDNPLFITVLETVYHNIHTYYERYLPMEETVLRQNYQDLKDLFAAVAARDGKRAEELAREHVRRFNTRMEERHIS
>Mature_238_residues
MFRKAKQNRIFQDVVDQIQEAIVEGRLKEGDQLPAERKLQEEFNVSRGTLREALRVLEQKGLIEIRTGVAGGSVIKGVST
EQVSESLALLIRYQKVPLAKLAEFREGVEGIVAALAANRATSEQTSRLQELLSTAGEYLEQGEPHWDAFIRTDEEFHLAL
AEMTDNPLFITVLETVYHNIHTYYERYLPMEETVLRQNYQDLKDLFAAVAARDGKRAEELAREHVRRFNTRMEERHIS

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=201, Percent_Identity=30.8457711442786, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790780, Length=230, Percent_Identity=26.9565217391304, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789621, Length=172, Percent_Identity=31.9767441860465, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994955, Length=223, Percent_Identity=28.2511210762332, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1789353, Length=228, Percent_Identity=28.9473684210526, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082208, Length=218, Percent_Identity=26.605504587156, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790032, Length=237, Percent_Identity=28.6919831223629, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787821, Length=210, Percent_Identity=27.6190476190476, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27313; Mature: 27313

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKAKQNRIFQDVVDQIQEAIVEGRLKEGDQLPAERKLQEEFNVSRGTLREALRVLEQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
GLIEIRTGVAGGSVIKGVSTEQVSESLALLIRYQKVPLAKLAEFREGVEGIVAALAANRA
CCEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
TSEQTSRLQELLSTAGEYLEQGEPHWDAFIRTDEEFHLALAEMTDNPLFITVLETVYHNI
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
HTYYERYLPMEETVLRQNYQDLKDLFAAVAARDGKRAEELAREHVRRFNTRMEERHIS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFRKAKQNRIFQDVVDQIQEAIVEGRLKEGDQLPAERKLQEEFNVSRGTLREALRVLEQK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
GLIEIRTGVAGGSVIKGVSTEQVSESLALLIRYQKVPLAKLAEFREGVEGIVAALAANRA
CCEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
TSEQTSRLQELLSTAGEYLEQGEPHWDAFIRTDEEFHLALAEMTDNPLFITVLETVYHNI
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
HTYYERYLPMEETVLRQNYQDLKDLFAAVAARDGKRAEELAREHVRRFNTRMEERHIS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]