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The map label for this gene is pleD [H]

Identifier: 39998439

GI number: 39998439

Start: 3681280

End: 3682977

Strand: Direct

Name: pleD [H]

Synonym: GSU3350

Alternate gene names: 39998439

Gene position: 3681280-3682977 (Clockwise)

Preceding gene: 39998438

Following gene: 39998440

Centisome position: 96.52

GC content: 65.19

Gene sequence:

>1698_bases
ATGGAGCGGACCGAGGAACTGGAGCGCGTCAACGAGCGCCTCTTCCTCGCCGATCAGGTAAAGACCGACTTTCTGTCCCA
CATGTCGCGGGAGTTCCAGCGCCCTCTCAACCATATTGTGGATTTCGCCCAGTGTCTGCGGGAAGGGGCCATGGGGCCGG
TCACCCGCGAGCAGCGGATCTGCCTCGACACGATTATTGCCCGGGGCAAGAGGCTTCAGCGGCTGCTGGAAAGGGTCCTC
TCCCTCTGCAACGACGAGCTGGGGATGGCCCTGTTCCTGCCCCGGAAGTTCCCGGTGCGCGAAGTGCTGGACCACGTCAT
GGACAGCCTGCGGGGAGCGGCCGGAAAGCGGGGGGTGGAGCTCCATGCCTGCTGCGGCGAAGACGGATGCACCATCACTG
CCGACAAGGGGAAATTCACCTTCATCATCGAAGAGTTGCTGACCAATGCCCTCAAGTTCTCGGACCGGGGAGGACGGGTG
GACGTCGCCATCGACCCCGTAAAACGGCGCGGGAGAGGCGAAGAGCTCCGGATCACGGTTTCTGACCAAGGGAGAGGTAT
CGGCAGCAAGGAGCTTGAGCAGATCTTCCGGAGCTTCGAGATGGGAAAACGGGGGCAGGCAGAGCCGGGCAGCCTCGGCA
TCGGGCTCACCCTGGTCAAACGTTTCGTGGAGCTGCATGGCGGCACTATAGCCGTGGAGAGCACCCCCGGCATCGGGAGT
ACCTTCACGGTCGTGCTCCCCCGCCAAGGCCCCGCTGAACGGGTCGGTCCACCGCCCCGGGTTATCCTGGCCGACCAGGA
CCCTGAACGGCTCCGGGAGCTGGCCGATGCATTACGACAGGAGCGGTGTGACATCATTACCGCCGTGGACGGGCGGGACG
CCCTCGACAAGGGGATCGCACAGACACCGGCGCTCTGCATCCTCGCCCTCGACTTGCCGGGAATCGACGGAATCGACGTC
TGCTCCCGTCTTAAGGCCCATGAACAGAGCTCCCAGATTCCGGTGATCATCACCGCGCCCCACCGGGATGCATCAGTTCG
GTCCCGGTGTGCCCAGGCGGGAGCCGACGGATTTTTCGCTCTCCCCGCCGAAATGGGCGATCTCCTCATGGCGGCGCGGG
GACTCATTGCCCGAAAACTCAGCTACGACTTCCTGAAGCGCAACTATGAGATTGCCGCCAGCGAGGCCTTCACCGATCCC
CTCACGGGGCTCTTCAACCTCCGACAGTTCTGGCTCAGCCTCGACCACGAACTGAAGCGCGCCCGCCGCTACCGCCGCAA
CTGCTCGCTGGCCATGATCGACATCGATTGGTTCAAGCAGTACAACGACTGCCACGGCCACCTCCGGGGCGACGAGGTCC
TCAGGGAGGCTGCCGCCGTGTTCCGCGGCTGCATCAGGACCTCGGACATCGCGGCACGCTACGGGGGGGAGGAGTTCGTC
GTCATCATGCCCGAAACCACCAGGGAGCTGGCTCTGCTGGCCGCCGAGAAGCTGCGCCGGGCCGTGGAAGAGCATCTCTT
CCCGCTCCGGGAGACCCAGCCCGGCGGCGCTCTCACGGTCAGCATCGGCATCGCCACCTTCCCCGACGACGCCGAGGACG
GGCGGGAACTGGTCGAGGCCGCCGACCGGGCCCTCTACCGGGCAAAGGAAGGGGGGCGGAACCGGGTGGTTGCCGCAGTG
GCGGACGGAACCGATTGA

Upstream 100 bases:

>100_bases
CCCCATACCACTCGCTGGCCCCTTTCCTGAGGCAGTGACCGTGACGCGCCGGCGCACGATCGTCAGTTCGCGGGAGCTCG
ACCTCCTGCGCCGCCTGCTC

Downstream 100 bases:

>100_bases
GACCGGGGGACCGGGTGATATACTGAACGTTAAGGAGAACGAGGAACTACCGAACTCCCGCATCCTCTCAGGGAAAGGAG
ATGGAGCACATGTCACGGGT

Product: sensor histidine kinase/GGDEF domain protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MERTEELERVNERLFLADQVKTDFLSHMSREFQRPLNHIVDFAQCLREGAMGPVTREQRICLDTIIARGKRLQRLLERVL
SLCNDELGMALFLPRKFPVREVLDHVMDSLRGAAGKRGVELHACCGEDGCTITADKGKFTFIIEELLTNALKFSDRGGRV
DVAIDPVKRRGRGEELRITVSDQGRGIGSKELEQIFRSFEMGKRGQAEPGSLGIGLTLVKRFVELHGGTIAVESTPGIGS
TFTVVLPRQGPAERVGPPPRVILADQDPERLRELADALRQERCDIITAVDGRDALDKGIAQTPALCILALDLPGIDGIDV
CSRLKAHEQSSQIPVIITAPHRDASVRSRCAQAGADGFFALPAEMGDLLMAARGLIARKLSYDFLKRNYEIAASEAFTDP
LTGLFNLRQFWLSLDHELKRARRYRRNCSLAMIDIDWFKQYNDCHGHLRGDEVLREAAAVFRGCIRTSDIAARYGGEEFV
VIMPETTRELALLAAEKLRRAVEEHLFPLRETQPGGALTVSIGIATFPDDAEDGRELVEAADRALYRAKEGGRNRVVAAV
ADGTD

Sequences:

>Translated_565_residues
MERTEELERVNERLFLADQVKTDFLSHMSREFQRPLNHIVDFAQCLREGAMGPVTREQRICLDTIIARGKRLQRLLERVL
SLCNDELGMALFLPRKFPVREVLDHVMDSLRGAAGKRGVELHACCGEDGCTITADKGKFTFIIEELLTNALKFSDRGGRV
DVAIDPVKRRGRGEELRITVSDQGRGIGSKELEQIFRSFEMGKRGQAEPGSLGIGLTLVKRFVELHGGTIAVESTPGIGS
TFTVVLPRQGPAERVGPPPRVILADQDPERLRELADALRQERCDIITAVDGRDALDKGIAQTPALCILALDLPGIDGIDV
CSRLKAHEQSSQIPVIITAPHRDASVRSRCAQAGADGFFALPAEMGDLLMAARGLIARKLSYDFLKRNYEIAASEAFTDP
LTGLFNLRQFWLSLDHELKRARRYRRNCSLAMIDIDWFKQYNDCHGHLRGDEVLREAAAVFRGCIRTSDIAARYGGEEFV
VIMPETTRELALLAAEKLRRAVEEHLFPLRETQPGGALTVSIGIATFPDDAEDGRELVEAADRALYRAKEGGRNRVVAAV
ADGTD
>Mature_565_residues
MERTEELERVNERLFLADQVKTDFLSHMSREFQRPLNHIVDFAQCLREGAMGPVTREQRICLDTIIARGKRLQRLLERVL
SLCNDELGMALFLPRKFPVREVLDHVMDSLRGAAGKRGVELHACCGEDGCTITADKGKFTFIIEELLTNALKFSDRGGRV
DVAIDPVKRRGRGEELRITVSDQGRGIGSKELEQIFRSFEMGKRGQAEPGSLGIGLTLVKRFVELHGGTIAVESTPGIGS
TFTVVLPRQGPAERVGPPPRVILADQDPERLRELADALRQERCDIITAVDGRDALDKGIAQTPALCILALDLPGIDGIDV
CSRLKAHEQSSQIPVIITAPHRDASVRSRCAQAGADGFFALPAEMGDLLMAARGLIARKLSYDFLKRNYEIAASEAFTDP
LTGLFNLRQFWLSLDHELKRARRYRRNCSLAMIDIDWFKQYNDCHGHLRGDEVLREAAAVFRGCIRTSDIAARYGGEEFV
VIMPETTRELALLAAEKLRRAVEEHLFPLRETQPGGALTVSIGIATFPDDAEDGRELVEAADRALYRAKEGGRNRVVAAV
ADGTD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=365, Percent_Identity=25.4794520547945, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081816, Length=355, Percent_Identity=28.7323943661972, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=40.3409090909091, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=35.0574712643678, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=38.3233532934132, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=27.1966527196653, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=38.4146341463415, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI145693157, Length=255, Percent_Identity=28.6274509803922, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=34.0540540540541, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=37.9746835443038, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=33.9622641509434, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=35.625, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=33.7349397590361, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994928, Length=474, Percent_Identity=23.6286919831224, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=28.6363636363636, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081974, Length=169, Percent_Identity=28.4023668639053, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=25.3112033195021, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=28.9308176100629, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=23.8938053097345, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62649; Mature: 62649

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTEELERVNERLFLADQVKTDFLSHMSREFQRPLNHIVDFAQCLREGAMGPVTREQRI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
CLDTIIARGKRLQRLLERVLSLCNDELGMALFLPRKFPVREVLDHVMDSLRGAAGKRGVE
HHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCE
LHACCGEDGCTITADKGKFTFIIEELLTNALKFSDRGGRVDVAIDPVKRRGRGEELRITV
EEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCEEEEEE
SDQGRGIGSKELEQIFRSFEMGKRGQAEPGSLGIGLTLVKRFVELHGGTIAVESTPGIGS
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
TFTVVLPRQGPAERVGPPPRVILADQDPERLRELADALRQERCDIITAVDGRDALDKGIA
EEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCC
QTPALCILALDLPGIDGIDVCSRLKAHEQSSQIPVIITAPHRDASVRSRCAQAGADGFFA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCEEE
LPAEMGDLLMAARGLIARKLSYDFLKRNYEIAASEAFTDPLTGLFNLRQFWLSLDHELKR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRYRRNCSLAMIDIDWFKQYNDCHGHLRGDEVLREAAAVFRGCIRTSDIAARYGGEEFV
HHHHHHCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VIMPETTRELALLAAEKLRRAVEEHLFPLRETQPGGALTVSIGIATFPDDAEDGRELVEA
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHH
ADRALYRAKEGGRNRVVAAVADGTD
HHHHHHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure
MERTEELERVNERLFLADQVKTDFLSHMSREFQRPLNHIVDFAQCLREGAMGPVTREQRI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
CLDTIIARGKRLQRLLERVLSLCNDELGMALFLPRKFPVREVLDHVMDSLRGAAGKRGVE
HHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCE
LHACCGEDGCTITADKGKFTFIIEELLTNALKFSDRGGRVDVAIDPVKRRGRGEELRITV
EEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHCCCCCEEEEEE
SDQGRGIGSKELEQIFRSFEMGKRGQAEPGSLGIGLTLVKRFVELHGGTIAVESTPGIGS
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
TFTVVLPRQGPAERVGPPPRVILADQDPERLRELADALRQERCDIITAVDGRDALDKGIA
EEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCC
QTPALCILALDLPGIDGIDVCSRLKAHEQSSQIPVIITAPHRDASVRSRCAQAGADGFFA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCEEE
LPAEMGDLLMAARGLIARKLSYDFLKRNYEIAASEAFTDPLTGLFNLRQFWLSLDHELKR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRYRRNCSLAMIDIDWFKQYNDCHGHLRGDEVLREAAAVFRGCIRTSDIAARYGGEEFV
HHHHHHCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VIMPETTRELALLAAEKLRRAVEEHLFPLRETQPGGALTVSIGIATFPDDAEDGRELVEA
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHH
ADRALYRAKEGGRNRVVAAVADGTD
HHHHHHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA