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The map label for this gene is nirB [C]

Identifier: 39998355

GI number: 39998355

Start: 3581132

End: 3581809

Strand: Direct

Name: nirB [C]

Synonym: GSU3265

Alternate gene names: 39998355

Gene position: 3581132-3581809 (Clockwise)

Preceding gene: 39998354

Following gene: 308513340

Centisome position: 93.89

GC content: 62.24

Gene sequence:

>678_bases
ATGAAAAAAGACATCCTTGAAAAGGGGGCCATCCTCCAGCGTGACCGGGAGACCTACGCCATCGCCCCCCACATCCCCGG
CGGGATCACCGATACGGCAACCCTGCGCAAGCTCTGCGATGTGGCCGACAAGTACGGCGTGAAGGAGTTGAAGCTCACCT
CTGCCCAACGCATCGCCCTCTTCGGCGTAAAGGAGGAGGATCTGGACGCCATCTGGTCCGATCTCGACCAGCAGCCGGGC
GCAGCCATCGGCCTCTGCGTCCGGAGCGTCAAGATCTGTCCCGGCACCACCTGGTGCAAGCGGGGTGTGCAGGATTCGGT
CTCCCTGGGATTGAAGATCGATGCCATCTACCACGCCATGGAACTGCCCAACAAAATGAAGATGGGGATTTCAGGATGCA
TGCTCAACTGCGCCGAGACCATGCTGAAGGATGTGGGAGTGGTCGGTACTCCCAAGGGGTGGCGGATCTACGTGGGGGGC
AATGCGGGGGCACGGCCCCGCATCGGCGACCTCCTGGTGGAACTGGGTCCGGATGAAGACAAGGTTCTCGCCGTGATTGC
GGACCTTGTGGAGTGGTACCGCTCTTCGGGCAGCGAGCAGCGGCTGGGCCGCGTGGTGGAGCAGATGGGGTTCGACGCCT
TCAAAACCGCCGTGCTGGGAGCGAAAGCAACCCCCTGA

Upstream 100 bases:

>100_bases
GCCACGGCCTGTATCGCCCGCATCCGCCCGAAGCAGGCCGCGCCGCAGCTTCAGGCCGGCGACCCGTGATACCCCATGCA
TGTTCGGAAAAGGAGTAATC

Downstream 100 bases:

>100_bases
GCCCCGCGGCTGACAGCGATTCCGCCGGTATGATCAAAAAAGGTCCAGCTGCTCCCCCTTCGGCTTCCGCCGGCCGCCAC
GGCCCGGCTCCGGCTGCCGG

Product: sulfite reductase, assimilatory-type

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKKDILEKGAILQRDRETYAIAPHIPGGITDTATLRKLCDVADKYGVKELKLTSAQRIALFGVKEEDLDAIWSDLDQQPG
AAIGLCVRSVKICPGTTWCKRGVQDSVSLGLKIDAIYHAMELPNKMKMGISGCMLNCAETMLKDVGVVGTPKGWRIYVGG
NAGARPRIGDLLVELGPDEDKVLAVIADLVEWYRSSGSEQRLGRVVEQMGFDAFKTAVLGAKATP

Sequences:

>Translated_225_residues
MKKDILEKGAILQRDRETYAIAPHIPGGITDTATLRKLCDVADKYGVKELKLTSAQRIALFGVKEEDLDAIWSDLDQQPG
AAIGLCVRSVKICPGTTWCKRGVQDSVSLGLKIDAIYHAMELPNKMKMGISGCMLNCAETMLKDVGVVGTPKGWRIYVGG
NAGARPRIGDLLVELGPDEDKVLAVIADLVEWYRSSGSEQRLGRVVEQMGFDAFKTAVLGAKATP
>Mature_225_residues
MKKDILEKGAILQRDRETYAIAPHIPGGITDTATLRKLCDVADKYGVKELKLTSAQRIALFGVKEEDLDAIWSDLDQQPG
AAIGLCVRSVKICPGTTWCKRGVQDSVSLGLKIDAIYHAMELPNKMKMGISGCMLNCAETMLKDVGVVGTPKGWRIYVGG
NAGARPRIGDLLVELGPDEDKVLAVIADLVEWYRSSGSEQRLGRVVEQMGFDAFKTAVLGAKATP

Specific function: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789765, Length=219, Percent_Identity=35.1598173515982, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR017220 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 24362; Mature: 24362

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDILEKGAILQRDRETYAIAPHIPGGITDTATLRKLCDVADKYGVKELKLTSAQRIAL
CCHHHHHCCHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCCEEEE
FGVKEEDLDAIWSDLDQQPGAAIGLCVRSVKICPGTTWCKRGVQDSVSLGLKIDAIYHAM
ECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCHHHHHHHHH
ELPNKMKMGISGCMLNCAETMLKDVGVVGTPKGWRIYVGGNAGARPRIGDLLVELGPDED
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCHH
KVLAVIADLVEWYRSSGSEQRLGRVVEQMGFDAFKTAVLGAKATP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKDILEKGAILQRDRETYAIAPHIPGGITDTATLRKLCDVADKYGVKELKLTSAQRIAL
CCHHHHHCCHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCCEEEE
FGVKEEDLDAIWSDLDQQPGAAIGLCVRSVKICPGTTWCKRGVQDSVSLGLKIDAIYHAM
ECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCHHHHHHHHH
ELPNKMKMGISGCMLNCAETMLKDVGVVGTPKGWRIYVGGNAGARPRIGDLLVELGPDED
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCHH
KVLAVIADLVEWYRSSGSEQRLGRVVEQMGFDAFKTAVLGAKATP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1911781 [H]