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The map label for this gene is cafA [H]

Identifier: 39998329

GI number: 39998329

Start: 3551672

End: 3553267

Strand: Reverse

Name: cafA [H]

Synonym: GSU3239

Alternate gene names: 39998329

Gene position: 3553267-3551672 (Counterclockwise)

Preceding gene: 39998330

Following gene: 39998328

Centisome position: 93.16

GC content: 58.77

Gene sequence:

>1596_bases
TTGCGCCGGAAGCCCTGGCTGAGGCCAGGATCGAGAAGCTCCAGGTGCTTTTCAAGGAATCGTGATTCAATTTTTCATAC
AGGATACCCATCAATGGCCAATGAACTGGTGATCAACACCTCGTCCCACGAGACCCGCATCGCTCTCATCGAGAACGGCA
CCATTGCCGAGCTCTACATTGAGCGGAGCCGCGAAAAGGGGATCGTCGGCAACATCTACAAGGGGAGGGTCGTACGCGTC
CTTCCCGGCATGCAGGCGGCATTTGTCGACATCGGCCTGGAGAAGGCGGCTTTTCTCTACGTGGCCGATGTGTTCGATGC
CATGGAGGAGTTCGAGACCTTCATGGAGGTGAACGGCGAGGAGAGCGACAGTGAACGGCAGGTTCTTCACCCGCTCCACC
CCATCGAAGAACTCCTTCAGGAAGGGCAGGAAATTCTGGTTCAGGTGTCCAAGGAACCCATCGGCACCAAGGGGGCCCGT
ATCACCTCTCATGTGTCGCTGCCTGGCCGCCACCTGGTCTACATGCCGACGGTGGACCACGTGGGAGTGTCTCGGCGGAT
CGAAGATGAGCCGGAGCGCGAGCGGCTTAAGGAAATCGTGGGCAAGGCGAAGCCGGCCGGGGGCGGGTTCATCGTTCGGA
CCGCCGCCGAGGGCAAGACCGAGGAGGATCTGGTCGCCGACATGAACTACCTGACGAAGCTCTGGGAAGAGGTTGTCAGA
CGCAACGAAAAGGCTCATGCCCCCTGTCTCATTCATGCCGACCTGGACGTGACCCAGAAGGTCATCCGTGACATCCTCAC
CGAAAACGTTTCCCGCATCGTGGTCGACTCCCGGCACGAGCACGACAAGATCGTCCAGTTCATCTCCACCTTCATGCCGA
AAATGAAGTACTCCATGGAGCTCTACGACGACGAGGAGCCCATCTTTGATCACTTCGGGCTGGAGGTGGAGATCAGCCGG
GCACTGGGACGCAAGGTCTGGCTCAAGAGCGGCGGCTATATCATCATCGAGCAGACCGAGGCCCTTACCGCCATCGATGT
CAACACCGGCCGGTTCGTGGGCAAACACAACCTGGAGGACACCATCCTCAAGACCAACCTGGAGGCGGTTAAGGAGATCG
CCTACCAGCTCAGGCTGCGCAATATCGGCGGGATCATCATCATCGACTTCATCGACATGGAGAAGGAGGTCAACCGGGAG
AAGGTCTTTACGGCGCTGGAGGAGGCCATGAAGGCCGACAAGTCCAAGACCAACATCCTGAAGATCTCGGATCTGGGCCT
GGTGGAGATGACCCGGAAGCGGGTGCGGGAGTCTATCGGCAGGATGATGTGCGAGCCCTGCCCCTACTGCGAGGGGAGAG
GGTACGTGAAGTCCAAGACAACGGTCTGTCACGAGATATTCCGCGAACTGCGCCGGGAGATGCTCGATATTCGCGGCAGC
AAGGTAATGCTCACCGTGCATCCCCAGGTGGCCGATCTCCTCTACGACGAGGAGCGGCGAGGGTTGGAGGAGTTGGAGAA
GAAGTTCAAGAAGCGGATTACGGTCCGGGCCAAGCCCGGTTTTCATCAGGAGCAGTTCGAGATCGCCATCAGCTGA

Upstream 100 bases:

>100_bases
CTGCGCCGGGAGCTGGCCGAACTCACCGTGGAGGGGAACGCCCTGGAGATGGTGACGGGGCGGGGCAAACCGCTGGAGTT
CGTATCGGCCATCACCGGGC

Downstream 100 bases:

>100_bases
CGGCAGGCTCAAGGGGGACGCGCATGGTATTCGCCGCCAAGGTTTCCGAAATCCCCGATTTCGGGAAAAAAGTTGTCACC
GTTAATGGACAGGAGATTCT

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MRRKPWLRPGSRSSRCFSRNRDSIFHTGYPSMANELVINTSSHETRIALIENGTIAELYIERSREKGIVGNIYKGRVVRV
LPGMQAAFVDIGLEKAAFLYVADVFDAMEEFETFMEVNGEESDSERQVLHPLHPIEELLQEGQEILVQVSKEPIGTKGAR
ITSHVSLPGRHLVYMPTVDHVGVSRRIEDEPERERLKEIVGKAKPAGGGFIVRTAAEGKTEEDLVADMNYLTKLWEEVVR
RNEKAHAPCLIHADLDVTQKVIRDILTENVSRIVVDSRHEHDKIVQFISTFMPKMKYSMELYDDEEPIFDHFGLEVEISR
ALGRKVWLKSGGYIIIEQTEALTAIDVNTGRFVGKHNLEDTILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKEVNRE
KVFTALEEAMKADKSKTNILKISDLGLVEMTRKRVRESIGRMMCEPCPYCEGRGYVKSKTTVCHEIFRELRREMLDIRGS
KVMLTVHPQVADLLYDEERRGLEELEKKFKKRITVRAKPGFHQEQFEIAIS

Sequences:

>Translated_531_residues
MRRKPWLRPGSRSSRCFSRNRDSIFHTGYPSMANELVINTSSHETRIALIENGTIAELYIERSREKGIVGNIYKGRVVRV
LPGMQAAFVDIGLEKAAFLYVADVFDAMEEFETFMEVNGEESDSERQVLHPLHPIEELLQEGQEILVQVSKEPIGTKGAR
ITSHVSLPGRHLVYMPTVDHVGVSRRIEDEPERERLKEIVGKAKPAGGGFIVRTAAEGKTEEDLVADMNYLTKLWEEVVR
RNEKAHAPCLIHADLDVTQKVIRDILTENVSRIVVDSRHEHDKIVQFISTFMPKMKYSMELYDDEEPIFDHFGLEVEISR
ALGRKVWLKSGGYIIIEQTEALTAIDVNTGRFVGKHNLEDTILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKEVNRE
KVFTALEEAMKADKSKTNILKISDLGLVEMTRKRVRESIGRMMCEPCPYCEGRGYVKSKTTVCHEIFRELRREMLDIRGS
KVMLTVHPQVADLLYDEERRGLEELEKKFKKRITVRAKPGFHQEQFEIAIS
>Mature_531_residues
MRRKPWLRPGSRSSRCFSRNRDSIFHTGYPSMANELVINTSSHETRIALIENGTIAELYIERSREKGIVGNIYKGRVVRV
LPGMQAAFVDIGLEKAAFLYVADVFDAMEEFETFMEVNGEESDSERQVLHPLHPIEELLQEGQEILVQVSKEPIGTKGAR
ITSHVSLPGRHLVYMPTVDHVGVSRRIEDEPERERLKEIVGKAKPAGGGFIVRTAAEGKTEEDLVADMNYLTKLWEEVVR
RNEKAHAPCLIHADLDVTQKVIRDILTENVSRIVVDSRHEHDKIVQFISTFMPKMKYSMELYDDEEPIFDHFGLEVEISR
ALGRKVWLKSGGYIIIEQTEALTAIDVNTGRFVGKHNLEDTILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKEVNRE
KVFTALEEAMKADKSKTNILKISDLGLVEMTRKRVRESIGRMMCEPCPYCEGRGYVKSKTTVCHEIFRELRREMLDIRGS
KVMLTVHPQVADLLYDEERRGLEELEKKFKKRITVRAKPGFHQEQFEIAIS

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=499, Percent_Identity=49.2985971943888, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787325, Length=480, Percent_Identity=36.875, Blast_Score=297, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 60866; Mature: 60866

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKPWLRPGSRSSRCFSRNRDSIFHTGYPSMANELVINTSSHETRIALIENGTIAELYI
CCCCCCCCCCCCCCHHHHCCCCCEEECCCCHHCCCEEEECCCCCEEEEEEECCCEEEHEE
ERSREKGIVGNIYKGRVVRVLPGMQAAFVDIGLEKAAFLYVADVFDAMEEFETFMEVNGE
HHHCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ESDSERQVLHPLHPIEELLQEGQEILVQVSKEPIGTKGARITSHVSLPGRHLVYMPTVDH
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
VGVSRRIEDEPERERLKEIVGKAKPAGGGFIVRTAAEGKTEEDLVADMNYLTKLWEEVVR
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
RNEKAHAPCLIHADLDVTQKVIRDILTENVSRIVVDSRHEHDKIVQFISTFMPKMKYSME
CCCCCCCCEEEECCHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHCEEE
LYDDEEPIFDHFGLEVEISRALGRKVWLKSGGYIIIEQTEALTAIDVNTGRFVGKHNLED
ECCCCCCHHHHCCCEEEEHHHHCCEEEEECCCEEEEECCCCEEEEECCCCCEEECCCCHH
TILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKEVNREKVFTALEEAMKADKSKTNIL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCCEE
KISDLGLVEMTRKRVRESIGRMMCEPCPYCEGRGYVKSKTTVCHEIFRELRREMLDIRGS
EEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCC
KVMLTVHPQVADLLYDEERRGLEELEKKFKKRITVRAKPGFHQEQFEIAIS
EEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCCCCCEEEEEC
>Mature Secondary Structure
MRRKPWLRPGSRSSRCFSRNRDSIFHTGYPSMANELVINTSSHETRIALIENGTIAELYI
CCCCCCCCCCCCCCHHHHCCCCCEEECCCCHHCCCEEEECCCCCEEEEEEECCCEEEHEE
ERSREKGIVGNIYKGRVVRVLPGMQAAFVDIGLEKAAFLYVADVFDAMEEFETFMEVNGE
HHHCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ESDSERQVLHPLHPIEELLQEGQEILVQVSKEPIGTKGARITSHVSLPGRHLVYMPTVDH
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
VGVSRRIEDEPERERLKEIVGKAKPAGGGFIVRTAAEGKTEEDLVADMNYLTKLWEEVVR
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
RNEKAHAPCLIHADLDVTQKVIRDILTENVSRIVVDSRHEHDKIVQFISTFMPKMKYSME
CCCCCCCCEEEECCHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHCEEE
LYDDEEPIFDHFGLEVEISRALGRKVWLKSGGYIIIEQTEALTAIDVNTGRFVGKHNLED
ECCCCCCHHHHCCCEEEEHHHHCCEEEEECCCEEEEECCCCEEEEECCCCCEEECCCCHH
TILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKEVNREKVFTALEEAMKADKSKTNIL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCCEE
KISDLGLVEMTRKRVRESIGRMMCEPCPYCEGRGYVKSKTTVCHEIFRELRREMLDIRGS
EEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCC
KVMLTVHPQVADLLYDEERRGLEELEKKFKKRITVRAKPGFHQEQFEIAIS
EEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]