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The map label for this gene is moaA

Identifier: 39998236

GI number: 39998236

Start: 3453478

End: 3454458

Strand: Reverse

Name: moaA

Synonym: GSU3146

Alternate gene names: 39998236

Gene position: 3454458-3453478 (Counterclockwise)

Preceding gene: 308513339

Following gene: 39998235

Centisome position: 90.57

GC content: 62.59

Gene sequence:

>981_bases
ATGGAACTCATTGACAGCTTTGGTCGCCGCATCAACTATCTCCGCCTCTCGGTCACCGACAGGTGCAATCTCCGCTGCAG
TTACTGCATGCCGGCCGAGGGGGTGGAAAAGCTCGCCCATGGCGACATCCTTTCCTACGAGGACCTTTTCCGCATTGCCC
GGGCCGCCGTTGCCATAGGCATCGAGAAGATCCGGATCACCGGCGGCGAACCCCTGGTCCGCAAGGGAATCGTGCCGTTC
CTGGCCCGCATTGCCGCCATTGAGGGGCTTCGGCAGCTGGTCCTCACTACCAATGGTCTCTTGTTGCCGGAGATGGCGGC
CGATCTCCGGAGCGCGGGTGTCCAGCGGCTCAACATCAGCCTTGACTCGCTCAGGGCCGACACCTTCCGGGCCATCACCA
GGATCGGAGAGCTGCAACGGGTGCTCGACGGCATCGCCGCCGCCGACGCGGCCGGTTTTCCTCCTCCCAAGATCAATATG
GTGGTCATGCGCGGTGTCAATGACGGTGAAGTGGCCGATTTTGCCCGCCTCACCATTGACCGTCCCTGCACGGTGCGTTT
CATCGAGTACATGCCGGCCACGCGGGAGAACAACTGGCAGTCCCTGACGGTGCCTGGCCGGGAAATCCTCGACCGGATCT
CCGCATCGTACGAGCTGGAGCCGGTGGAGAAAGGGGCATGTGCCGGACCGTCCAGGGATTTCAGGATTCGTGGCGCTGCC
GGGACACTCGGCGTGATAACCGCCGTTTCCGGGCACTTCTGCGGCGATTGTAACCGCGTCAGGGTCACCTCGACCGGCAT
GGCCAAGAGCTGTCTCTTCTCGGATGAAGGGTTCGACCTGCGTCCGTTCCTCGAAACCAGCGATCCGATTATCCTTCAGG
AGGCGCTGCGGCGAATCGTGGGGGTCAAGCCCGAGCGCCACGGCATGAGCGCATGCAAAGCGGAGCACCAGGCGTTTTCC
ATGGCGAAGATCGGCGGATAA

Upstream 100 bases:

>100_bases
CGCAACGGAGTTGACGGTTCCGTATACGCTCACCTCTATCACGGGTCATTCTGCTATAGTAACAAAAATGCAACGTCGTA
CCTTTTCGCCTGGATTTCCC

Downstream 100 bases:

>100_bases
CAGTGCCGGAAGCACCACAACGAGAGGGTTTCATGTCAGCGAGAGTAGTTGCCGTCTGTATCAGCAGGAACAAGGGGGAG
CGCAAGACGCCGGTGACGGG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MELIDSFGRRINYLRLSVTDRCNLRCSYCMPAEGVEKLAHGDILSYEDLFRIARAAVAIGIEKIRITGGEPLVRKGIVPF
LARIAAIEGLRQLVLTTNGLLLPEMAADLRSAGVQRLNISLDSLRADTFRAITRIGELQRVLDGIAAADAAGFPPPKINM
VVMRGVNDGEVADFARLTIDRPCTVRFIEYMPATRENNWQSLTVPGREILDRISASYELEPVEKGACAGPSRDFRIRGAA
GTLGVITAVSGHFCGDCNRVRVTSTGMAKSCLFSDEGFDLRPFLETSDPIILQEALRRIVGVKPERHGMSACKAEHQAFS
MAKIGG

Sequences:

>Translated_326_residues
MELIDSFGRRINYLRLSVTDRCNLRCSYCMPAEGVEKLAHGDILSYEDLFRIARAAVAIGIEKIRITGGEPLVRKGIVPF
LARIAAIEGLRQLVLTTNGLLLPEMAADLRSAGVQRLNISLDSLRADTFRAITRIGELQRVLDGIAAADAAGFPPPKINM
VVMRGVNDGEVADFARLTIDRPCTVRFIEYMPATRENNWQSLTVPGREILDRISASYELEPVEKGACAGPSRDFRIRGAA
GTLGVITAVSGHFCGDCNRVRVTSTGMAKSCLFSDEGFDLRPFLETSDPIILQEALRRIVGVKPERHGMSACKAEHQAFS
MAKIGG
>Mature_326_residues
MELIDSFGRRINYLRLSVTDRCNLRCSYCMPAEGVEKLAHGDILSYEDLFRIARAAVAIGIEKIRITGGEPLVRKGIVPF
LARIAAIEGLRQLVLTTNGLLLPEMAADLRSAGVQRLNISLDSLRADTFRAITRIGELQRVLDGIAAADAAGFPPPKINM
VVMRGVNDGEVADFARLTIDRPCTVRFIEYMPATRENNWQSLTVPGREILDRISASYELEPVEKGACAGPSRDFRIRGAA
GTLGVITAVSGHFCGDCNRVRVTSTGMAKSCLFSDEGFDLRPFLETSDPIILQEALRRIVGVKPERHGMSACKAEHQAFS
MAKIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=331, Percent_Identity=42.5981873111783, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI22027536, Length=331, Percent_Identity=42.5981873111783, Blast_Score=241, Evalue=8e-64,
Organism=Escherichia coli, GI1786999, Length=287, Percent_Identity=36.2369337979094, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32566329, Length=314, Percent_Identity=37.5796178343949, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=36.8932038834951, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17561210, Length=316, Percent_Identity=35.7594936708861, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI28574490, Length=323, Percent_Identity=41.7956656346749, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI28574488, Length=332, Percent_Identity=41.2650602409639, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_GEOSL (Q747W9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954187.1
- ProteinModelPortal:   Q747W9
- SMR:   Q747W9
- GeneID:   2688414
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3146
- NMPDR:   fig|243231.1.peg.3125
- TIGR:   GSU3146
- HOGENOM:   HBG529885
- OMA:   FFRAMSR
- ProtClustDB:   CLSK829137
- BioCyc:   GSUL243231:GSU_3146-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 35622; Mature: 35622

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 15-15 BINDING 28-28 BINDING 65-65 BINDING 69-69 BINDING 96-96 BINDING 120-120 BINDING 157-157 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIDSFGRRINYLRLSVTDRCNLRCSYCMPAEGVEKLAHGDILSYEDLFRIARAAVAIG
CCHHHHHCCCEEEEEEEECCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IEKIRITGGEPLVRKGIVPFLARIAAIEGLRQLVLTTNGLLLPEMAADLRSAGVQRLNIS
HEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECC
LDSLRADTFRAITRIGELQRVLDGIAAADAAGFPPPKINMVVMRGVNDGEVADFARLTID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHEEC
RPCTVRFIEYMPATRENNWQSLTVPGREILDRISASYELEPVEKGACAGPSRDFRIRGAA
CCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCC
GTLGVITAVSGHFCGDCNRVRVTSTGMAKSCLFSDEGFDLRPFLETSDPIILQEALRRIV
CCHHHHHHHCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHH
GVKPERHGMSACKAEHQAFSMAKIGG
CCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELIDSFGRRINYLRLSVTDRCNLRCSYCMPAEGVEKLAHGDILSYEDLFRIARAAVAIG
CCHHHHHCCCEEEEEEEECCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IEKIRITGGEPLVRKGIVPFLARIAAIEGLRQLVLTTNGLLLPEMAADLRSAGVQRLNIS
HEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECC
LDSLRADTFRAITRIGELQRVLDGIAAADAAGFPPPKINMVVMRGVNDGEVADFARLTID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHEEC
RPCTVRFIEYMPATRENNWQSLTVPGREILDRISASYELEPVEKGACAGPSRDFRIRGAA
CCCHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCC
GTLGVITAVSGHFCGDCNRVRVTSTGMAKSCLFSDEGFDLRPFLETSDPIILQEALRRIV
CCHHHHHHHCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHH
GVKPERHGMSACKAEHQAFSMAKIGG
CCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA