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The map label for this gene is mraW

Identifier: 39998167

GI number: 39998167

Start: 3385043

End: 3385978

Strand: Reverse

Name: mraW

Synonym: GSU3077

Alternate gene names: 39998167

Gene position: 3385978-3385043 (Counterclockwise)

Preceding gene: 39998168

Following gene: 39998166

Centisome position: 88.77

GC content: 63.03

Gene sequence:

>936_bases
ATGACGTTTTCGCACCGTCCGGTCATGCCGGCCGAGGTGCTGGAGTATTTATCCCCCCGCCCCGGTGGGGTGTATGTGGA
CGGAACGCTGGGGGGGGCGGGGCATGCCCGGCTGATCCTTGAAGCGACGTCCCCTGATGGGATGTTGATCGGGTTTGACC
GTGACCCTGCCGCCCTGGAGGTCGCTCGGGAGCGACTTGCATTATTTGGTGAGCGCTTCAGGCCTGTCCCCGGCAACTTT
TCGGAAATGGGCCGCGTTCTGGCCGAGCTGGGAGTTGACGGTGTCGACGGATTTCTCCTGGATGTGGGGGTCTCCTCACA
TCAGCTGGACACGGCGGAGCGGGGGTTCAGCTTTCTGACCGATGCGCCTCTCGATATGCGGATGAATACCTTGGTGCCCG
GCACCGCCGCGGACCTGGTCAACGATCTAAACGAACACGAGCTGGCCCGCATCATAAAAGAATATGGCGAAGAGCGATGG
GCGCGGCGAATCGCCTCGTTCATCGTCAAGGCCCGGGTTGACGGACCAATCGAGCGAACGCTCCAGTTGGTCGACATCAT
CAAAGGGGCAATTCCGCGGGGAGCCTGGGAAGAGCGGATTCACCCGGCTACCCGGACCTTTCAGGCCCTTCGGATAGCGG
TGAACGACGAACTTGGAAGCCTGGAGCGGGGCCTTGAATCCGCCCTGGGGCTGTTGAGGCCTGGCGGACGGGGGGTTGTG
ATCTCCTTTCACTCGCTGGAGGACCGGATCGTTAAAACCATGTTCCGCCGTTACGCCCAGGGGTGCACCTGTCCGAAGGA
GCTTCCCCGCTGCGTGTGTGGCGGTGTTCCCCGGCTCCGTATCCTGACGGGACGGCCGGTAGTGGCGGGTGACGTCGAGG
TGGCGGAGAACCCGAGAGCGCGCAGCGCCAAGCTGCGGGCGGCAGAAAAACTGTAG

Upstream 100 bases:

>100_bases
AGAAGTGTGGAGCCAAGCGGAGCGGGAGAAGGTTCGGATTCAGGATCTGAAGAATTTCCCGTCCGACTCTGAGACAGTAG
CAGAGTTGGGGCTCTAGGTG

Downstream 100 bases:

>100_bases
TATTTGCGGATGCTGAAGGAGGCGGAGATGGCGCAGGCACGGACGGAATTCAGCAAGGTTGCGGCTCCCAGGAAGCTGGA
GGAGATGTACGCCCAGCGGG

Product: S-adenosyl-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTFSHRPVMPAEVLEYLSPRPGGVYVDGTLGGAGHARLILEATSPDGMLIGFDRDPAALEVARERLALFGERFRPVPGNF
SEMGRVLAELGVDGVDGFLLDVGVSSHQLDTAERGFSFLTDAPLDMRMNTLVPGTAADLVNDLNEHELARIIKEYGEERW
ARRIASFIVKARVDGPIERTLQLVDIIKGAIPRGAWEERIHPATRTFQALRIAVNDELGSLERGLESALGLLRPGGRGVV
ISFHSLEDRIVKTMFRRYAQGCTCPKELPRCVCGGVPRLRILTGRPVVAGDVEVAENPRARSAKLRAAEKL

Sequences:

>Translated_311_residues
MTFSHRPVMPAEVLEYLSPRPGGVYVDGTLGGAGHARLILEATSPDGMLIGFDRDPAALEVARERLALFGERFRPVPGNF
SEMGRVLAELGVDGVDGFLLDVGVSSHQLDTAERGFSFLTDAPLDMRMNTLVPGTAADLVNDLNEHELARIIKEYGEERW
ARRIASFIVKARVDGPIERTLQLVDIIKGAIPRGAWEERIHPATRTFQALRIAVNDELGSLERGLESALGLLRPGGRGVV
ISFHSLEDRIVKTMFRRYAQGCTCPKELPRCVCGGVPRLRILTGRPVVAGDVEVAENPRARSAKLRAAEKL
>Mature_310_residues
TFSHRPVMPAEVLEYLSPRPGGVYVDGTLGGAGHARLILEATSPDGMLIGFDRDPAALEVARERLALFGERFRPVPGNFS
EMGRVLAELGVDGVDGFLLDVGVSSHQLDTAERGFSFLTDAPLDMRMNTLVPGTAADLVNDLNEHELARIIKEYGEERWA
RRIASFIVKARVDGPIERTLQLVDIIKGAIPRGAWEERIHPATRTFQALRIAVNDELGSLERGLESALGLLRPGGRGVVI
SFHSLEDRIVKTMFRRYAQGCTCPKELPRCVCGGVPRLRILTGRPVVAGDVEVAENPRARSAKLRAAEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=44.213649851632, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI165377202, Length=208, Percent_Identity=43.2692307692308, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=45.8860759493671, Blast_Score=249, Evalue=2e-67,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=40.9638554216867, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=40.9638554216867, Blast_Score=197, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_GEOSL (P60396)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954118.1
- ProteinModelPortal:   P60396
- GeneID:   2688487
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3077
- NMPDR:   fig|243231.1.peg.3056
- TIGR:   GSU3077
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   GSUL243231:GSU_3077-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34038; Mature: 33907

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 80-80 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSHRPVMPAEVLEYLSPRPGGVYVDGTLGGAGHARLILEATSPDGMLIGFDRDPAALE
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCHHHHH
VARERLALFGERFRPVPGNFSEMGRVLAELGVDGVDGFLLDVGVSSHQLDTAERGFSFLT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHCCHHHHCCHHHC
DAPLDMRMNTLVPGTAADLVNDLNEHELARIIKEYGEERWARRIASFIVKARVDGPIERT
CCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQLVDIIKGAIPRGAWEERIHPATRTFQALRIAVNDELGSLERGLESALGLLRPGGRGVV
HHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
ISFHSLEDRIVKTMFRRYAQGCTCPKELPRCVCGGVPRLRILTGRPVVAGDVEVAENPRA
EEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCEEECCCHHCCCCCC
RSAKLRAAEKL
HHHHHHHHHCC
>Mature Secondary Structure 
TFSHRPVMPAEVLEYLSPRPGGVYVDGTLGGAGHARLILEATSPDGMLIGFDRDPAALE
CCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCHHHHH
VARERLALFGERFRPVPGNFSEMGRVLAELGVDGVDGFLLDVGVSSHQLDTAERGFSFLT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHCCHHHHCCHHHC
DAPLDMRMNTLVPGTAADLVNDLNEHELARIIKEYGEERWARRIASFIVKARVDGPIERT
CCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQLVDIIKGAIPRGAWEERIHPATRTFQALRIAVNDELGSLERGLESALGLLRPGGRGVV
HHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
ISFHSLEDRIVKTMFRRYAQGCTCPKELPRCVCGGVPRLRILTGRPVVAGDVEVAENPRA
EEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCEEECCCHHCCCCCC
RSAKLRAAEKL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA