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The map label for this gene is ddl

Identifier: 39998157

GI number: 39998157

Start: 3371784

End: 3372734

Strand: Reverse

Name: ddl

Synonym: GSU3066

Alternate gene names: 39998157

Gene position: 3372734-3371784 (Counterclockwise)

Preceding gene: 39998158

Following gene: 39998156

Centisome position: 88.43

GC content: 62.78

Gene sequence:

>951_bases
ATGACGAGGGATGAGCTGAAAACCACGAAGATAGGCGTACTTATGGGAGGACTCTCGGCTGAGCGCGAAGTGTCCCTTGC
CAGCGGCGGCGCTGTGCTGAAGGCCCTCCAGAGCCGGGGATATGACGCGGTCCCCGTGGACGTCGGCCGGGATCTCCCGC
AGGTTCTGGTCCGGGAGTGGATCGATGTTGCTTTTATCTGTCTCCACGGCCGCTACGGGGAAGACGGAACCGTCCAGGGG
CTGCTGGAGCTCATGGGCATTCCCTACACCGGATCGGGGGTCCTGGCCAGCGCCCTTGCCATGAACAAGATCGTCGCCAA
GGAGGTTTTCGCCGCGCGGGGGCTCACGATCGCTCCCTACCGGGTTGTGCGCCGGGGCGAGACAGTTGATCCCGTGGCGG
AAGGGTTCGGCTACCCGGTGGTGGTGAAGCCGTCTCAGGAAGGGTCATCGGTGGGGGTGAGCATCGTCAAGTCGCCGGAA
GAGCTCCCTTCTGCCCTGGAGCTCGCCTTCCGCTATGATGACGATATCCTGGTGGAGCGCTTCATCAAGGGGCGCGAGAT
CCAGATCGGGATACTGGACGACCGGGCCATGGGGGCCATCGAGATCGTTCCCGTGAACGAGTTCTACGACTTCGAGGCCA
AGTATACCGCCGGCAAGGCCGAGCATATCTGCCCACCGGTACTCCCGGCGGAACTGCACCGCAGGCTCCTGGCCGAAGGG
GAAGCGGCTCACCGCGCCCTGGGATGCTCCGGTTACAGCAGAGTCGATTTCCTCGTAACGGAAGGGGGCGAGTGTTATCT
CCTGGAGGTGAACACCCTGCCGGGAATGACCGCGTTGAGCCTTCTGCCCGAGATCGCCCTCAAGGAGTCCGGGATCGGTT
TCGAGGACCTGGTGGAGAGAATTCTGATTTCCGCTGAGCTGAAAATCAAGGGGGAAGGGGCCGGAAGCTAG

Upstream 100 bases:

>100_bases
TCAAGGATGCGGTGAAGCTCAAAAGCGGCACGGCTCTTGAAGAAGAAGTCAAAATTTTCGGCGATGAGTAAGGGGCTGTA
GTGTCCCGCGCGGAGCGGCA

Downstream 100 bases:

>100_bases
GCGCGGAGCATATGCGCGATCTGCACGTCAAGACACGGATAAAGCCGGCCCAGACCCGCAACCGCGTGAAGCGGGAGCGT
AAGCCGATCAACTGGCGCCC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTRDELKTTKIGVLMGGLSAEREVSLASGGAVLKALQSRGYDAVPVDVGRDLPQVLVREWIDVAFICLHGRYGEDGTVQG
LLELMGIPYTGSGVLASALAMNKIVAKEVFAARGLTIAPYRVVRRGETVDPVAEGFGYPVVVKPSQEGSSVGVSIVKSPE
ELPSALELAFRYDDDILVERFIKGREIQIGILDDRAMGAIEIVPVNEFYDFEAKYTAGKAEHICPPVLPAELHRRLLAEG
EAAHRALGCSGYSRVDFLVTEGGECYLLEVNTLPGMTALSLLPEIALKESGIGFEDLVERILISAELKIKGEGAGS

Sequences:

>Translated_316_residues
MTRDELKTTKIGVLMGGLSAEREVSLASGGAVLKALQSRGYDAVPVDVGRDLPQVLVREWIDVAFICLHGRYGEDGTVQG
LLELMGIPYTGSGVLASALAMNKIVAKEVFAARGLTIAPYRVVRRGETVDPVAEGFGYPVVVKPSQEGSSVGVSIVKSPE
ELPSALELAFRYDDDILVERFIKGREIQIGILDDRAMGAIEIVPVNEFYDFEAKYTAGKAEHICPPVLPAELHRRLLAEG
EAAHRALGCSGYSRVDFLVTEGGECYLLEVNTLPGMTALSLLPEIALKESGIGFEDLVERILISAELKIKGEGAGS
>Mature_315_residues
TRDELKTTKIGVLMGGLSAEREVSLASGGAVLKALQSRGYDAVPVDVGRDLPQVLVREWIDVAFICLHGRYGEDGTVQGL
LELMGIPYTGSGVLASALAMNKIVAKEVFAARGLTIAPYRVVRRGETVDPVAEGFGYPVVVKPSQEGSSVGVSIVKSPEE
LPSALELAFRYDDDILVERFIKGREIQIGILDDRAMGAIEIVPVNEFYDFEAKYTAGKAEHICPPVLPAELHRRLLAEGE
AAHRALGCSGYSRVDFLVTEGGECYLLEVNTLPGMTALSLLPEIALKESGIGFEDLVERILISAELKIKGEGAGS

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=46.5346534653465, Blast_Score=254, Evalue=3e-69,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=32.7586206896552, Blast_Score=184, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_GEOSL (Q748D8)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954108.1
- HSSP:   P07862
- ProteinModelPortal:   Q748D8
- SMR:   Q748D8
- GeneID:   2686321
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3066
- NMPDR:   fig|243231.1.peg.3046
- TIGR:   GSU3066
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- ProtClustDB:   CLSK829085
- BioCyc:   GSUL243231:GSU_3066-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33931; Mature: 33799

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDELKTTKIGVLMGGLSAEREVSLASGGAVLKALQSRGYDAVPVDVGRDLPQVLVREW
CCHHHHHHHEEEEEECCCCCCCEEEECCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
IDVAFICLHGRYGEDGTVQGLLELMGIPYTGSGVLASALAMNKIVAKEVFAARGLTIAPY
HHHEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCH
RVVRRGETVDPVAEGFGYPVVVKPSQEGSSVGVSIVKSPEELPSALELAFRYDDDILVER
HHHHCCCCCCHHHHCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHH
FIKGREIQIGILDDRAMGAIEIVPVNEFYDFEAKYTAGKAEHICPPVLPAELHRRLLAEG
HHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHCC
EAAHRALGCSGYSRVDFLVTEGGECYLLEVNTLPGMTALSLLPEIALKESGIGFEDLVER
HHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ILISAELKIKGEGAGS
HHHHEEEEEECCCCCC
>Mature Secondary Structure 
TRDELKTTKIGVLMGGLSAEREVSLASGGAVLKALQSRGYDAVPVDVGRDLPQVLVREW
CHHHHHHHEEEEEECCCCCCCEEEECCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
IDVAFICLHGRYGEDGTVQGLLELMGIPYTGSGVLASALAMNKIVAKEVFAARGLTIAPY
HHHEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCH
RVVRRGETVDPVAEGFGYPVVVKPSQEGSSVGVSIVKSPEELPSALELAFRYDDDILVER
HHHHCCCCCCHHHHCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHH
FIKGREIQIGILDDRAMGAIEIVPVNEFYDFEAKYTAGKAEHICPPVLPAELHRRLLAEG
HHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHCC
EAAHRALGCSGYSRVDFLVTEGGECYLLEVNTLPGMTALSLLPEIALKESGIGFEDLVER
HHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
ILISAELKIKGEGAGS
HHHHEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA