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The map label for this gene is gltA [H]

Identifier: 39998148

GI number: 39998148

Start: 3360147

End: 3361559

Strand: Reverse

Name: gltA [H]

Synonym: GSU3057

Alternate gene names: 39998148

Gene position: 3361559-3360147 (Counterclockwise)

Preceding gene: 39998149

Following gene: 39998147

Centisome position: 88.13

GC content: 66.03

Gene sequence:

>1413_bases
ATGAGCACGGGTATGACCACCAGAGAGCGATTGGCCATTGACCGGGTCCACATGGTCGAACTCGATGCCGACAGCCGCAG
CCGAAATTTTGACGAAGTGAACCGGGGCCTCACCCCGGAGGAGGCCGTGCGCGAGGCCCAGCGCTGCATTCGCTGCACGA
ACCGCCAGTGCGTGGCAGGCTGTCCCGTGGGGGTGTCGATCCCGGAATTTATCACGGCCCTGGCCGACGGCAACCTCAGG
GAGGCTGCCCGGATCCTGACCAGGGACAATGCGTTGCCTGCCGTGTGTGGCCGGGTCTGTCCCCAGGAAACCCAATGCGA
GGCCAGGTGCGTGCGTGGGATCAAGGGAGAGCCCGTGGCCATCGGGTATCTGGAGCGTTTTGTGGCGGACTGGGCCACGG
CCAATGTCGCCGAGCTCGGTGACGAGCCGCTGCCGCCGCCAACAGGCAGGCGGGTGGCAGTGGTCGGCTGTGGTCCGGCC
GGATTGACTGCCGCCGGGGAGTTGGCCCGCAAGGGACATGGCGTGACTATCTTCGAGGCGCTCCACGACACGGGCGGGGT
GCTTCGCTACGGCATTCCGGAGTTCCGCCTGCCCAAAACCATTATCGATGCCGAGGTGGCGCGGCTTCTTGCACTGGGGG
TGACGGTTGAGTGCAATGTCATCATCGGCAAGACGCTGACCCTCGCCCAGCTGCGGGAAGAATTCGACGCGGTATTCATC
GCCAATGGCGCCGGCCTGCCGGTGATGCTCTCCATCCCGGGCGAGAATCTCAAGGGGGTCTATTCCGCCAACGAATACCT
GACCCGGGTTAACCTCATGGGGGCCGGCCGCGACCCCGGCTCCACCACTCCCATCATTGCCGGTAAGCGCGTGGCCGTCA
TCGGCGGGGGCAACACCGCCATGGACTGCGTCCGAACCGCTCGCCGTCTGGGCGCCGAGCGGGCCATGATCATCTACCGC
AGGAGCGAGGAGCAGATGCCGGCCCGGGTGGAGGAGATCAAGCACGCCAAGGAGGAAGGGGTCGAGTTCGTAATGCTGAC
AGCCCCCATCGCCATCGGGGGCGACAAGGACGGGTGGGTGGCCACCCTGCGCTGCCTGCGAATGGAGCTGGGCGCGCCCG
ATTCGTCGGGACGCCGATCACCGGTGCCGGTGGAAGGTTCCGCATACGACATTGCCGTGGATGTGGTGGTGAATGCCGTC
GGTACCCGGGCCAATCCGCTCCTGACCGCCACGGCGCCGGAACTTAAACTGAACCGGTGGGGCAACATCGCCACCGATGA
CAACGGTGCCACGAGCGTTGCCGGCGTCTATGCCGGTGGCGACATCGTGCGAGGGGGCGCCACGGTAATCCTTGCCATGG
GCGATGGGAAGCAGGCGGCTGCCGCGATCCACGACTGGCTGAACAGGCGGTGA

Upstream 100 bases:

>100_bases
ACGGCCATCTGGTGGATTTCGACAACCTGGCCGATCGGCTGGCAACCTATCGCGAGTCCGAGGCCAGTCACGTCTGCCGG
GTCGCCGGAAAGGAGGTCGC

Downstream 100 bases:

>100_bases
GCTGATGACGGACTGTCCGTGTCCGTAACCGTGCGGGCGCCAAGCGCGTAACAGTGCGCTGCGGACAAAAAAAAGCCGCT
CCTCCAGAGGGGGCGGCTTT

Product: oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSTGMTTRERLAIDRVHMVELDADSRSRNFDEVNRGLTPEEAVREAQRCIRCTNRQCVAGCPVGVSIPEFITALADGNLR
EAARILTRDNALPAVCGRVCPQETQCEARCVRGIKGEPVAIGYLERFVADWATANVAELGDEPLPPPTGRRVAVVGCGPA
GLTAAGELARKGHGVTIFEALHDTGGVLRYGIPEFRLPKTIIDAEVARLLALGVTVECNVIIGKTLTLAQLREEFDAVFI
ANGAGLPVMLSIPGENLKGVYSANEYLTRVNLMGAGRDPGSTTPIIAGKRVAVIGGGNTAMDCVRTARRLGAERAMIIYR
RSEEQMPARVEEIKHAKEEGVEFVMLTAPIAIGGDKDGWVATLRCLRMELGAPDSSGRRSPVPVEGSAYDIAVDVVVNAV
GTRANPLLTATAPELKLNRWGNIATDDNGATSVAGVYAGGDIVRGGATVILAMGDGKQAAAAIHDWLNRR

Sequences:

>Translated_470_residues
MSTGMTTRERLAIDRVHMVELDADSRSRNFDEVNRGLTPEEAVREAQRCIRCTNRQCVAGCPVGVSIPEFITALADGNLR
EAARILTRDNALPAVCGRVCPQETQCEARCVRGIKGEPVAIGYLERFVADWATANVAELGDEPLPPPTGRRVAVVGCGPA
GLTAAGELARKGHGVTIFEALHDTGGVLRYGIPEFRLPKTIIDAEVARLLALGVTVECNVIIGKTLTLAQLREEFDAVFI
ANGAGLPVMLSIPGENLKGVYSANEYLTRVNLMGAGRDPGSTTPIIAGKRVAVIGGGNTAMDCVRTARRLGAERAMIIYR
RSEEQMPARVEEIKHAKEEGVEFVMLTAPIAIGGDKDGWVATLRCLRMELGAPDSSGRRSPVPVEGSAYDIAVDVVVNAV
GTRANPLLTATAPELKLNRWGNIATDDNGATSVAGVYAGGDIVRGGATVILAMGDGKQAAAAIHDWLNRR
>Mature_469_residues
STGMTTRERLAIDRVHMVELDADSRSRNFDEVNRGLTPEEAVREAQRCIRCTNRQCVAGCPVGVSIPEFITALADGNLRE
AARILTRDNALPAVCGRVCPQETQCEARCVRGIKGEPVAIGYLERFVADWATANVAELGDEPLPPPTGRRVAVVGCGPAG
LTAAGELARKGHGVTIFEALHDTGGVLRYGIPEFRLPKTIIDAEVARLLALGVTVECNVIIGKTLTLAQLREEFDAVFIA
NGAGLPVMLSIPGENLKGVYSANEYLTRVNLMGAGRDPGSTTPIIAGKRVAVIGGGNTAMDCVRTARRLGAERAMIIYRR
SEEQMPARVEEIKHAKEEGVEFVMLTAPIAIGGDKDGWVATLRCLRMELGAPDSSGRRSPVPVEGSAYDIAVDVVVNAVG
TRANPLLTATAPELKLNRWGNIATDDNGATSVAGVYAGGDIVRGGATVILAMGDGKQAAAAIHDWLNRR

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=475, Percent_Identity=31.5789473684211, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI237757300, Length=112, Percent_Identity=33.0357142857143, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=455, Percent_Identity=37.3626373626374, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082180, Length=448, Percent_Identity=37.9464285714286, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1789606, Length=451, Percent_Identity=36.3636363636364, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1788468, Length=448, Percent_Identity=34.5982142857143, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789243, Length=456, Percent_Identity=33.5526315789474, Blast_Score=198, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI71984108, Length=454, Percent_Identity=32.8193832599119, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=30.6288032454361, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17543860, Length=159, Percent_Identity=33.9622641509434, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=31.340206185567, Blast_Score=203, Evalue=4e-53,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=31.1340206185567, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=31.1340206185567, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=31.1340206185567, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=31.1340206185567, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24640763, Length=463, Percent_Identity=30.2375809935205, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI18858217, Length=463, Percent_Identity=30.2375809935205, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI17137178, Length=169, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 49951; Mature: 49820

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGMTTRERLAIDRVHMVELDADSRSRNFDEVNRGLTPEEAVREAQRCIRCTNRQCVAG
CCCCCCCHHHHHHCEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEC
CPVGVSIPEFITALADGNLREAARILTRDNALPAVCGRVCPQETQCEARCVRGIKGEPVA
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHCCCCCCCEE
IGYLERFVADWATANVAELGDEPLPPPTGRRVAVVGCGPAGLTAAGELARKGHGVTIFEA
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEE
LHDTGGVLRYGIPEFRLPKTIIDAEVARLLALGVTVECNVIIGKTLTLAQLREEFDAVFI
HHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHCEEEE
ANGAGLPVMLSIPGENLKGVYSANEYLTRVNLMGAGRDPGSTTPIIAGKRVAVIGGGNTA
ECCCCCEEEEECCCCCCCEEECHHHHHEEEEEEECCCCCCCCCCEEECCEEEEEECCCHH
MDCVRTARRLGAERAMIIYRRSEEQMPARVEEIKHAKEEGVEFVMLTAPIAIGGDKDGWV
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHH
ATLRCLRMELGAPDSSGRRSPVPVEGSAYDIAVDVVVNAVGTRANPLLTATAPELKLNRW
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCEEEECCCCEECCCC
GNIATDDNGATSVAGVYAGGDIVRGGATVILAMGDGKQAAAAIHDWLNRR
CCCCCCCCCCCEEEEEEECCCEEECCEEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
STGMTTRERLAIDRVHMVELDADSRSRNFDEVNRGLTPEEAVREAQRCIRCTNRQCVAG
CCCCCCHHHHHHCEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEC
CPVGVSIPEFITALADGNLREAARILTRDNALPAVCGRVCPQETQCEARCVRGIKGEPVA
CCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHCCCCCCCEE
IGYLERFVADWATANVAELGDEPLPPPTGRRVAVVGCGPAGLTAAGELARKGHGVTIFEA
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEE
LHDTGGVLRYGIPEFRLPKTIIDAEVARLLALGVTVECNVIIGKTLTLAQLREEFDAVFI
HHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHCEEEE
ANGAGLPVMLSIPGENLKGVYSANEYLTRVNLMGAGRDPGSTTPIIAGKRVAVIGGGNTA
ECCCCCEEEEECCCCCCCEEECHHHHHEEEEEEECCCCCCCCCCEEECCEEEEEECCCHH
MDCVRTARRLGAERAMIIYRRSEEQMPARVEEIKHAKEEGVEFVMLTAPIAIGGDKDGWV
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHH
ATLRCLRMELGAPDSSGRRSPVPVEGSAYDIAVDVVVNAVGTRANPLLTATAPELKLNRW
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCEEEECCCCEECCCC
GNIATDDNGATSVAGVYAGGDIVRGGATVILAMGDGKQAAAAIHDWLNRR
CCCCCCCCCCCEEEEEEECCCEEECCEEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]