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The map label for this gene is nuoE [H]

Identifier: 39997816

GI number: 39997816

Start: 2999397

End: 2999972

Strand: Reverse

Name: nuoE [H]

Synonym: GSU2722

Alternate gene names: 39997816

Gene position: 2999972-2999397 (Counterclockwise)

Preceding gene: 39997817

Following gene: 39997815

Centisome position: 78.65

GC content: 65.97

Gene sequence:

>576_bases
GTGAACTCCACGGATAATTCGCCAGGGAAGGGCACGCCCGACGGGCTTGCCGCCGATCCGCGCTTCAAGGTGGTCGAGCG
GACCCTGAAACAGTATCAGTACCGGGCCGATGCGCTCCTGGAGGTGCTTCACGTGGCCCAGGAGACCTTCGGCTGCCTCA
GCGACGAGCTGATGACCCATGTGGCCCGGCAACTCCGCGTTCCGCCCAGTCGCGTCTATGGCGTCGCCACCTTCTATCAT
TTCTTTACCCTTGAGGCCCGGGGCGAACACTCCTGCGTGGTCTGCACCGGCACCGCCTGCTATGTGAAGCGCTCGGCCGA
GATCGTGACGCGCCTCGAAAACGAGTTCGACGTGAAAGCCGGTAAAACCACGGCCGACGGCAAGCTTACCCTGTCGACGG
TCCGCTGCCTCGGCAGTTGCGGGCTGGCCCCCATTGTGGTCCTCGACGGGGAAACCGTGGGGCGCTGTACCCCCGATTCG
GCCGCCGCCGCCGTCCGGGTCATGCTGGCGGAAAAGAGCCCGGCCCGCGCAGAAACGGTCCGCCCGGTTCGCCGCCGCAA
GGGTGAAGGCCAATGA

Upstream 100 bases:

>100_bases
GCGGCCGCCTTGAACGCATAAGGCTTCACACATATTCCACGGTCTGTTAAGATGCGTGTTCGTCATCGCCCATTACCCCG
TTGCGGCAGAGGAGAACAGC

Downstream 100 bases:

>100_bases
ACCCCGCCGATCTCCATGCCATGGCCCGGGAAGAACAGGCCCGCCAGGAGGCGCTGCGGTGCCGTATCATGGTCTGTGCC
GGTACCCCCTGTCTTTCCGC

Product: bidirectional hydrogenase complex protein HoxE

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MNSTDNSPGKGTPDGLAADPRFKVVERTLKQYQYRADALLEVLHVAQETFGCLSDELMTHVARQLRVPPSRVYGVATFYH
FFTLEARGEHSCVVCTGTACYVKRSAEIVTRLENEFDVKAGKTTADGKLTLSTVRCLGSCGLAPIVVLDGETVGRCTPDS
AAAAVRVMLAEKSPARAETVRPVRRRKGEGQ

Sequences:

>Translated_191_residues
MNSTDNSPGKGTPDGLAADPRFKVVERTLKQYQYRADALLEVLHVAQETFGCLSDELMTHVARQLRVPPSRVYGVATFYH
FFTLEARGEHSCVVCTGTACYVKRSAEIVTRLENEFDVKAGKTTADGKLTLSTVRCLGSCGLAPIVVLDGETVGRCTPDS
AAAAVRVMLAEKSPARAETVRPVRRRKGEGQ
>Mature_191_residues
MNSTDNSPGKGTPDGLAADPRFKVVERTLKQYQYRADALLEVLHVAQETFGCLSDELMTHVARQLRVPPSRVYGVATFYH
FFTLEARGEHSCVVCTGTACYVKRSAEIVTRLENEFDVKAGKTTADGKLTLSTVRCLGSCGLAPIVVLDGETVGRCTPDS
AAAAVRVMLAEKSPARAETVRPVRRRKGEGQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=150, Percent_Identity=36.6666666666667, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788621, Length=138, Percent_Identity=31.1594202898551, Blast_Score=81, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=142, Percent_Identity=34.5070422535211, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18859877, Length=170, Percent_Identity=33.5294117647059, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355353, Length=154, Percent_Identity=27.9220779220779, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20747; Mature: 20747

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTDNSPGKGTPDGLAADPRFKVVERTLKQYQYRADALLEVLHVAQETFGCLSDELMTH
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARQLRVPPSRVYGVATFYHFFTLEARGEHSCVVCTGTACYVKRSAEIVTRLENEFDVKA
HHHHHCCCHHHHHHHHHHHHHEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
GKTTADGKLTLSTVRCLGSCGLAPIVVLDGETVGRCTPDSAAAAVRVMLAEKSPARAETV
CCCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
RPVRRRKGEGQ
HHHHHCCCCCC
>Mature Secondary Structure
MNSTDNSPGKGTPDGLAADPRFKVVERTLKQYQYRADALLEVLHVAQETFGCLSDELMTH
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARQLRVPPSRVYGVATFYHFFTLEARGEHSCVVCTGTACYVKRSAEIVTRLENEFDVKA
HHHHHCCCHHHHHHHHHHHHHEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
GKTTADGKLTLSTVRCLGSCGLAPIVVLDGETVGRCTPDSAAAAVRVMLAEKSPARAETV
CCCCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHH
RPVRRRKGEGQ
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]