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The map label for this gene is macA [H]

Identifier: 39997759

GI number: 39997759

Start: 2939179

End: 2940339

Strand: Reverse

Name: macA [H]

Synonym: GSU2665

Alternate gene names: 39997759

Gene position: 2940339-2939179 (Counterclockwise)

Preceding gene: 39997760

Following gene: 39997758

Centisome position: 77.09

GC content: 65.37

Gene sequence:

>1161_bases
ATGACAGTCAAATGGTTGGTGTGCGTACTTGGCGGGGTGCTTCTGGCAGGCGGGCTTGCGGGATGTTCGAAGGATGGCGA
GGCACAGAACGCTCGGGTGGAAAAGCCGGCGCTGGCGGTGGAAACGGTTCCCGTGACCGCCCGCGATCTGGCGGAGGGAA
TCGAGGTTACCGGAAGCCTTGAGCCAAAATTTTCGGCCGACGTCAAAACTCAGATCCCCGGACTGGTAAAGCAGGTCTAC
GTTACGGAGTGGGTGCGGGTCCGCAAGGGGGCTCCCCTGGCGCGCATCGATACTGCCGAGCCGGAGGCCCTGACCAAGAG
GGCGGAGGCGTCGGTGGAATCTGCCCGGGCCGCGCTGGCCCAGGCCCAGGTAAGTGCCAACAGGGCCGAGCGGGAACTTG
CGCGGATCATCAAGCTGAAGGAGTCGGGGCTTGCCACCCAGCAGTCGGTGGACGATGCCCGCACCGAATCGGCCGCGGCC
CAGGCCAGGATCGATGCCGCCCAGGCCCAGATCCGGGTGACCCAGGAAGAGCTCCGGCAGGCGCGGGCCCGTCTGGCCAA
AGGGCTCGTGGTATCTCCGCTGGACGGAGTGGTGGCCCTGCGGGACGTAAACGTGGGAGACCTGGCCGGCGACGCGGCAA
CGGGCAAGCCGATCTTCCGTATCGTGGACAACAGGGTGCTCAACCTGACCGTTACCATCCCGTCGGCCGACTCGGCGCGT
GTCAAGGTCGGCCAGCCCCTGGAGTTTTCCGTGGATGCCCTGCCGGGGAGAATATTCACGGGCACGGTCATGTTCATCAA
TCCCGAACTCTCTGCCGAGGACCGCTCGCTGCGGGTCATCGCAGAGGTCCGCAATGACGGGGAACTCCTCAAGGGGGGGC
TCTTCGCCAAGGGGAGAATTCTTGTGGGCCGGCGGCCGGCGGTCGTTCAGGTGCCGAGAGGCTCCCTGGCCGCCTGGGAT
ACCGCCTCGAAGAAGGCGAGCATCTTCGTCGTTGCCGGCAACGTGGCGAAGATGAAGCAGGTGGAAACCGGGGTGGTGAT
CGGGGACGGAGTGGAGATCGTCAAGGGGGTCGGCCCCGGCGAACAACTCGTGACCAGGGGAGGCTTCAACCTGAAAGATG
GCGACGTCGTGACCGTCGTTGGGAAGGGCGCTGCGCAATGA

Upstream 100 bases:

>100_bases
GCCCGGACCGTCCGCCCGGCATTGACCGGGAGGGGGTGAGGCGTATGTTGACTCTTGTCCTGAGCGGATTTGCGGCCCGG
ACGGCCGACGAGGTGGAACG

Downstream 100 bases:

>100_bases
TCCTGTCAGACCTGTCCATCAAGCGTCCTATCTTCGCCACGGTGATGATGCTCGTGCTCGTCACGCTGGGGGCGTTTTCC
TATCGGCTGCTGTCGGTGGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTVKWLVCVLGGVLLAGGLAGCSKDGEAQNARVEKPALAVETVPVTARDLAEGIEVTGSLEPKFSADVKTQIPGLVKQVY
VTEWVRVRKGAPLARIDTAEPEALTKRAEASVESARAALAQAQVSANRAERELARIIKLKESGLATQQSVDDARTESAAA
QARIDAAQAQIRVTQEELRQARARLAKGLVVSPLDGVVALRDVNVGDLAGDAATGKPIFRIVDNRVLNLTVTIPSADSAR
VKVGQPLEFSVDALPGRIFTGTVMFINPELSAEDRSLRVIAEVRNDGELLKGGLFAKGRILVGRRPAVVQVPRGSLAAWD
TASKKASIFVVAGNVAKMKQVETGVVIGDGVEIVKGVGPGEQLVTRGGFNLKDGDVVTVVGKGAAQ

Sequences:

>Translated_386_residues
MTVKWLVCVLGGVLLAGGLAGCSKDGEAQNARVEKPALAVETVPVTARDLAEGIEVTGSLEPKFSADVKTQIPGLVKQVY
VTEWVRVRKGAPLARIDTAEPEALTKRAEASVESARAALAQAQVSANRAERELARIIKLKESGLATQQSVDDARTESAAA
QARIDAAQAQIRVTQEELRQARARLAKGLVVSPLDGVVALRDVNVGDLAGDAATGKPIFRIVDNRVLNLTVTIPSADSAR
VKVGQPLEFSVDALPGRIFTGTVMFINPELSAEDRSLRVIAEVRNDGELLKGGLFAKGRILVGRRPAVVQVPRGSLAAWD
TASKKASIFVVAGNVAKMKQVETGVVIGDGVEIVKGVGPGEQLVTRGGFNLKDGDVVTVVGKGAAQ
>Mature_385_residues
TVKWLVCVLGGVLLAGGLAGCSKDGEAQNARVEKPALAVETVPVTARDLAEGIEVTGSLEPKFSADVKTQIPGLVKQVYV
TEWVRVRKGAPLARIDTAEPEALTKRAEASVESARAALAQAQVSANRAERELARIIKLKESGLATQQSVDDARTESAAAQ
ARIDAAQAQIRVTQEELRQARARLAKGLVVSPLDGVVALRDVNVGDLAGDAATGKPIFRIVDNRVLNLTVTIPSADSARV
KVGQPLEFSVDALPGRIFTGTVMFINPELSAEDRSLRVIAEVRNDGELLKGGLFAKGRILVGRRPAVVQVPRGSLAAWDT
ASKKASIFVVAGNVAKMKQVETGVVIGDGVEIVKGVGPGEQLVTRGGFNLKDGDVVTVVGKGAAQ

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=25.9818731117825, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082036, Length=331, Percent_Identity=24.773413897281, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789929, Length=368, Percent_Identity=24.1847826086957, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40523; Mature: 40392

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKWLVCVLGGVLLAGGLAGCSKDGEAQNARVEKPALAVETVPVTARDLAEGIEVTGSL
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCEECCCC
EPKFSADVKTQIPGLVKQVYVTEWVRVRKGAPLARIDTAEPEALTKRAEASVESARAALA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
QAQVSANRAERELARIIKLKESGLATQQSVDDARTESAAAQARIDAAQAQIRVTQEELRQ
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHH
ARARLAKGLVVSPLDGVVALRDVNVGDLAGDAATGKPIFRIVDNRVLNLTVTIPSADSAR
HHHHHHCCCEECCCCCEEEEEECCCCCCCCCCCCCCHHHEEECCEEEEEEEEECCCCCCE
VKVGQPLEFSVDALPGRIFTGTVMFINPELSAEDRSLRVIAEVRNDGELLKGGLFAKGRI
EEECCCCEEEHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEECCEE
LVGRRPAVVQVPRGSLAAWDTASKKASIFVVAGNVAKMKQVETGVVIGDGVEIVKGVGPG
EECCCCCEEEECCCCEEECCCCCCCEEEEEEECCHHHHHHHCCCEEEECCHHEEECCCCC
EQLVTRGGFNLKDGDVVTVVGKGAAQ
HHHHHCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TVKWLVCVLGGVLLAGGLAGCSKDGEAQNARVEKPALAVETVPVTARDLAEGIEVTGSL
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCEECCCC
EPKFSADVKTQIPGLVKQVYVTEWVRVRKGAPLARIDTAEPEALTKRAEASVESARAALA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
QAQVSANRAERELARIIKLKESGLATQQSVDDARTESAAAQARIDAAQAQIRVTQEELRQ
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHH
ARARLAKGLVVSPLDGVVALRDVNVGDLAGDAATGKPIFRIVDNRVLNLTVTIPSADSAR
HHHHHHCCCEECCCCCEEEEEECCCCCCCCCCCCCCHHHEEECCEEEEEEEEECCCCCCE
VKVGQPLEFSVDALPGRIFTGTVMFINPELSAEDRSLRVIAEVRNDGELLKGGLFAKGRI
EEECCCCEEEHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEECCEE
LVGRRPAVVQVPRGSLAAWDTASKKASIFVVAGNVAKMKQVETGVVIGDGVEIVKGVGPG
EECCCCCEEEECCCCEEECCCCCCCEEEEEEECCHHHHHHHCCCEEEECCHHEEECCCCC
EQLVTRGGFNLKDGDVVTVVGKGAAQ
HHHHHCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]