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The map label for this gene is nirA [H]

Identifier: 39997622

GI number: 39997622

Start: 2784677

End: 2785978

Strand: Reverse

Name: nirA [H]

Synonym: GSU2527

Alternate gene names: 39997622

Gene position: 2785978-2784677 (Counterclockwise)

Preceding gene: 39997623

Following gene: 39997621

Centisome position: 73.04

GC content: 68.2

Gene sequence:

>1302_bases
ATGCGATTCGATCCGCAACAGCTCAGAATTGACGGCGTGTATGCCCAGCGACAACCCGGGACCTTCATGCTCCGGATAAA
GGTGCCGGCGGGCATTCTTTCAGCGGAGCAGGCCGAGGCGGTGGCCGACATCGCCGACCGGTTCGCCGGCGGCCTGCTCC
ATCTTACCACCAGGGGAAGCATCGAGCTGCACGGCCTCACCGAGGATCACCTTTCATCGGTCTGGCGCGGCCTTGCCGCC
GTGGGCCTCACCTCCCGCGGCGCCTGCGGCGGGGCGGTGCGCGGGGTGGTCTGCGGCTCGGTCACCGCCGCGGCCTGGCC
CCTGGTCCAGTCACTGGCCCACAAGCTCCATCTGCACTTTACCCGCAATCCCCATTTCGAGGCGCTGCCTAAAAAATTCA
AGATCGGCGTTTTTACCGGCGATAACGAGGGGCGGCACCTGATCCAGGACCTGTCGCTCGTGCACCGGGAGAACGAGGCC
GATGCACCGCGCTACGATGTCTGGGCAGCCGGCGGCCTCGGCCGGGAACCCTCGCCGGCATTCCTGGTGGAGGAGGCGGT
GGCCGAGGATCGGATCATCCCCCTGGTGGAGGCCATTGTCCGGGTCTACAAGGAGCGTGCGCCCAAGGGAAAGCGTCTCA
AGCATCTGGTCCGCGACCTGGGCCGCGAGGACTTCCTCGCCTTGGTGGCGGAGCGCCGGGGAGACCAGCCTCTGCCCCTG
GCCGACTCCTTCAGCGGCGAGCTGTTCCCGTCCGTCGCCGCCGGCGGCGTCTGCGTGGAGGCACCGGTCTTTGCCGGCGA
ACTGACAACGGGCGGCCTGCGGTCCTTGGCCGCCGTGGCCCGTGACGTTGCCGGCGGCTTCATGGTGCTCACCGGTGATC
AGAACGTGCGCTTCCTCCTGCCCGAATCCGTGCGCCGTCAGGATGCCCTCGACGCCGTGGCCGCCGCCGGATTCGCTGCG
GACCGTCCCGAACAGCGGGTAGCCTTCCGCATCTGCCCCGGCAGCCACGAATGCGCCATGGGACTCGCGCCGACCAGGGA
CGTGGCCCGGGCTGTTATCGGCGCCATGGGTGACGATGCCGCGGCGCTCCGGTGGTCCATATCCGGCTGCCCCAACTCCT
GCTCCCAGCCCCAGCTTGCCCGGGTGGGGATCGTCACCGCCAAGAGCGTCAAGGGAGAGGACGGACAACGCCGCCCCCTC
TTCGACCTCTATCGCCGCGAGGATGACGGCAGCCTTGGTGTTGTGGTCGGGAGCGGACTCGAACTGGAAGAACTCGTGGC
GGCCGTGCGTCTCGAACGCTGA

Upstream 100 bases:

>100_bases
TCGGGAATAGTGCGGTATATTTGGGTGGGTATTTTATGGTTTAAAGTGCAGTATTTTTGTGGTACTCTTTGGCTAAAATT
ATTTACCAAAGAGGTAGGCA

Downstream 100 bases:

>100_bases
CCGGTACCGCGTAGTTATGCCGGAGGGACGGATTGGCATGTAAATTGTAGATTTGCATTACCTAACGAGTTGTATTATAC
AGAACGAGGTAGTGCGAAAG

Product: nitrite/sulfite reductase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRFDPQQLRIDGVYAQRQPGTFMLRIKVPAGILSAEQAEAVADIADRFAGGLLHLTTRGSIELHGLTEDHLSSVWRGLAA
VGLTSRGACGGAVRGVVCGSVTAAAWPLVQSLAHKLHLHFTRNPHFEALPKKFKIGVFTGDNEGRHLIQDLSLVHRENEA
DAPRYDVWAAGGLGREPSPAFLVEEAVAEDRIIPLVEAIVRVYKERAPKGKRLKHLVRDLGREDFLALVAERRGDQPLPL
ADSFSGELFPSVAAGGVCVEAPVFAGELTTGGLRSLAAVARDVAGGFMVLTGDQNVRFLLPESVRRQDALDAVAAAGFAA
DRPEQRVAFRICPGSHECAMGLAPTRDVARAVIGAMGDDAAALRWSISGCPNSCSQPQLARVGIVTAKSVKGEDGQRRPL
FDLYRREDDGSLGVVVGSGLELEELVAAVRLER

Sequences:

>Translated_433_residues
MRFDPQQLRIDGVYAQRQPGTFMLRIKVPAGILSAEQAEAVADIADRFAGGLLHLTTRGSIELHGLTEDHLSSVWRGLAA
VGLTSRGACGGAVRGVVCGSVTAAAWPLVQSLAHKLHLHFTRNPHFEALPKKFKIGVFTGDNEGRHLIQDLSLVHRENEA
DAPRYDVWAAGGLGREPSPAFLVEEAVAEDRIIPLVEAIVRVYKERAPKGKRLKHLVRDLGREDFLALVAERRGDQPLPL
ADSFSGELFPSVAAGGVCVEAPVFAGELTTGGLRSLAAVARDVAGGFMVLTGDQNVRFLLPESVRRQDALDAVAAAGFAA
DRPEQRVAFRICPGSHECAMGLAPTRDVARAVIGAMGDDAAALRWSISGCPNSCSQPQLARVGIVTAKSVKGEDGQRRPL
FDLYRREDDGSLGVVVGSGLELEELVAAVRLER
>Mature_433_residues
MRFDPQQLRIDGVYAQRQPGTFMLRIKVPAGILSAEQAEAVADIADRFAGGLLHLTTRGSIELHGLTEDHLSSVWRGLAA
VGLTSRGACGGAVRGVVCGSVTAAAWPLVQSLAHKLHLHFTRNPHFEALPKKFKIGVFTGDNEGRHLIQDLSLVHRENEA
DAPRYDVWAAGGLGREPSPAFLVEEAVAEDRIIPLVEAIVRVYKERAPKGKRLKHLVRDLGREDFLALVAERRGDQPLPL
ADSFSGELFPSVAAGGVCVEAPVFAGELTTGGLRSLAAVARDVAGGFMVLTGDQNVRFLLPESVRRQDALDAVAAAGFAA
DRPEQRVAFRICPGSHECAMGLAPTRDVARAVIGAMGDDAAALRWSISGCPNSCSQPQLARVGIVTAKSVKGEDGQRRPL
FDLYRREDDGSLGVVVGSGLELEELVAAVRLER

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 46387; Mature: 46387

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDPQQLRIDGVYAQRQPGTFMLRIKVPAGILSAEQAEAVADIADRFAGGLLHLTTRGS
CCCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
IELHGLTEDHLSSVWRGLAAVGLTSRGACGGAVRGVVCGSVTAAAWPLVQSLAHKLHLHF
EEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEEEE
TRNPHFEALPKKFKIGVFTGDNEGRHLIQDLSLVHRENEADAPRYDVWAAGGLGREPSPA
CCCCCHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCH
FLVEEAVAEDRIIPLVEAIVRVYKERAPKGKRLKHLVRDLGREDFLALVAERRGDQPLPL
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
ADSFSGELFPSVAAGGVCVEAPVFAGELTTGGLRSLAAVARDVAGGFMVLTGDQNVRFLL
CCCCCCCCCHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEC
PESVRRQDALDAVAAAGFAADRPEQRVAFRICPGSHECAMGLAPTRDVARAVIGAMGDDA
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
AALRWSISGCPNSCSQPQLARVGIVTAKSVKGEDGQRRPLFDLYRREDDGSLGVVVGSGL
EEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCC
ELEELVAAVRLER
CHHHHHHHHHCCC
>Mature Secondary Structure
MRFDPQQLRIDGVYAQRQPGTFMLRIKVPAGILSAEQAEAVADIADRFAGGLLHLTTRGS
CCCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCC
IELHGLTEDHLSSVWRGLAAVGLTSRGACGGAVRGVVCGSVTAAAWPLVQSLAHKLHLHF
EEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHEEEEE
TRNPHFEALPKKFKIGVFTGDNEGRHLIQDLSLVHRENEADAPRYDVWAAGGLGREPSPA
CCCCCHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCH
FLVEEAVAEDRIIPLVEAIVRVYKERAPKGKRLKHLVRDLGREDFLALVAERRGDQPLPL
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
ADSFSGELFPSVAAGGVCVEAPVFAGELTTGGLRSLAAVARDVAGGFMVLTGDQNVRFLL
CCCCCCCCCHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEC
PESVRRQDALDAVAAAGFAADRPEQRVAFRICPGSHECAMGLAPTRDVARAVIGAMGDDA
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
AALRWSISGCPNSCSQPQLARVGIVTAKSVKGEDGQRRPLFDLYRREDDGSLGVVVGSGL
EEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCC
ELEELVAAVRLER
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]