Click here to switch to the map view.

The map label for this gene is sucB [H]

Identifier: 39997543

GI number: 39997543

Start: 2684931

End: 2686160

Strand: Reverse

Name: sucB [H]

Synonym: GSU2448

Alternate gene names: 39997543

Gene position: 2686160-2684931 (Counterclockwise)

Preceding gene: 39997544

Following gene: 39997542

Centisome position: 70.43

GC content: 66.99

Gene sequence:

>1230_bases
ATGGAAATCAAAATACCATCGGTGGGTGAATCGGTATTCGAGGCCCTGGTGGCCACGTGGCTCCGCCAGGACGGCGATGC
GGTCCGCAAGGACGAGCCGGTCTGCGAGATCGAGACCGACAAGATCACCATGGAGCTTAACGCTGAGGCCGACGGGGTTC
TCTCCATCGCGGTGCCGGCCGGGACCACGGTGAAGATCGGCACGGTCATCGGTACCATTCGCGAAGGCGCTGCCGCCCCC
GTTGCCGAGTCGCCGGCTCCGGCACAGGCCGCTGCCGCCGCGCCCGCCGCAGAGCCCCCTCTTTCCCCGTCGGTGCGTAA
AATGGCGCGGGAGCGGGGGATCAGCCCCGAGGCGGTGCCCGGTACCGGCCGGGGAGGGCGGGTGACGGTGGATGATCTCT
TCTCCTTTGCCGAAAAGCGGGAGCAGGGGGCGTCTCCGGCCGGGGCTCCTCCGCCGGCCGCTCCCCAGCCTCCCGCGCAG
CAGCCCCGGCCGGCGGCAGAGCAGCCCCGCCAGGCCGAGCCGCCGGAGGCCGATCGCACCACCCGCACCCCCATGACCCC
CATCCGCAAGCGGATCGCGGAACGTCTCATGGCGGCGCGTCAGCAGACCGCCATGCTCACCACCTTCAACGAGGCCGATC
TGGGGCGCATTGTGGAACTGCGTGCCCGCCACAAGGAGCAGTTCGCCAAGCGGCACGGGGTATCCTTGGGGTTCATGTCG
TTCTTCGTCAAGGCCTGCGTAGAGGCCCTGAAAGCCTTTCCCCTGGTGAACGCCCGCATCGACGGCAACGACATCGTCCG
GCACCACTACTACAACATCGGCATCGCCATCGGCGCGGACAAGGGGCTGGTGGTGCCGGTGCTCCGGGATGCGGACCGCC
TCCATTTCTGGGAGATCGAACAGGCCATCGCCGCCTTTGTCGAAAAGATCAAAACCAACCGGCTCGAACTCTCGGACCTG
GAGGGGGGCACCTTCTCCATAACCAACGGCGGGGTGTACGGCTCGCTCCTTTCCACCCCCATCCTCAATCCGCCCCAGAG
CGGGGTGCTCGGCATGCACGCCATTCAGGACCGTCCCGTGGCCCGGGACGGGCAGGTGGTCATCCGGCCCATGATGTACC
TGGCCCTGTCCTACGACCACCGGATCATCGACGGCCGCGAAGCGGTGGGATTCCTGCGCACGGTGAAAGAGTATGTGGAA
GATCCGGAGGAGCTGTTCCTTGAGGGGTAG

Upstream 100 bases:

>100_bases
TCACGGAGGCGCTCGCGGTATAGGCGTTCCCCCTCACCGGGGGCCGGTTCACGCATCGTGATAGGTGCTGATCGGCAGCA
CTGTTTCAACGGAGAATTCC

Downstream 100 bases:

>100_bases
GGTTGCGGTCATGGTGTGCCGGGTCGTGACGGCACTGGCCCTGTCGCTGCTGGCGGTTGCGCCGGTGCCGGCCGCTTCCC
GTACGGCAAAGGGCGCAAAG

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MEIKIPSVGESVFEALVATWLRQDGDAVRKDEPVCEIETDKITMELNAEADGVLSIAVPAGTTVKIGTVIGTIREGAAAP
VAESPAPAQAAAAAPAAEPPLSPSVRKMARERGISPEAVPGTGRGGRVTVDDLFSFAEKREQGASPAGAPPPAAPQPPAQ
QPRPAAEQPRQAEPPEADRTTRTPMTPIRKRIAERLMAARQQTAMLTTFNEADLGRIVELRARHKEQFAKRHGVSLGFMS
FFVKACVEALKAFPLVNARIDGNDIVRHHYYNIGIAIGADKGLVVPVLRDADRLHFWEIEQAIAAFVEKIKTNRLELSDL
EGGTFSITNGGVYGSLLSTPILNPPQSGVLGMHAIQDRPVARDGQVVIRPMMYLALSYDHRIIDGREAVGFLRTVKEYVE
DPEELFLEG

Sequences:

>Translated_409_residues
MEIKIPSVGESVFEALVATWLRQDGDAVRKDEPVCEIETDKITMELNAEADGVLSIAVPAGTTVKIGTVIGTIREGAAAP
VAESPAPAQAAAAAPAAEPPLSPSVRKMARERGISPEAVPGTGRGGRVTVDDLFSFAEKREQGASPAGAPPPAAPQPPAQ
QPRPAAEQPRQAEPPEADRTTRTPMTPIRKRIAERLMAARQQTAMLTTFNEADLGRIVELRARHKEQFAKRHGVSLGFMS
FFVKACVEALKAFPLVNARIDGNDIVRHHYYNIGIAIGADKGLVVPVLRDADRLHFWEIEQAIAAFVEKIKTNRLELSDL
EGGTFSITNGGVYGSLLSTPILNPPQSGVLGMHAIQDRPVARDGQVVIRPMMYLALSYDHRIIDGREAVGFLRTVKEYVE
DPEELFLEG
>Mature_409_residues
MEIKIPSVGESVFEALVATWLRQDGDAVRKDEPVCEIETDKITMELNAEADGVLSIAVPAGTTVKIGTVIGTIREGAAAP
VAESPAPAQAAAAAPAAEPPLSPSVRKMARERGISPEAVPGTGRGGRVTVDDLFSFAEKREQGASPAGAPPPAAPQPPAQ
QPRPAAEQPRQAEPPEADRTTRTPMTPIRKRIAERLMAARQQTAMLTTFNEADLGRIVELRARHKEQFAKRHGVSLGFMS
FFVKACVEALKAFPLVNARIDGNDIVRHHYYNIGIAIGADKGLVVPVLRDADRLHFWEIEQAIAAFVEKIKTNRLELSDL
EGGTFSITNGGVYGSLLSTPILNPPQSGVLGMHAIQDRPVARDGQVVIRPMMYLALSYDHRIIDGREAVGFLRTVKEYVE
DPEELFLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=55.0218340611354, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=27.122641509434, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI31711992, Length=447, Percent_Identity=26.6219239373602, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=26.0393873085339, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=25.6521739130435, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=30, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786946, Length=418, Percent_Identity=47.8468899521531, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=26.5588914549654, Blast_Score=141, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=414, Percent_Identity=38.4057971014493, Blast_Score=267, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=26.4018691588785, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=25.4945054945055, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=26.7741935483871, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=38.479809976247, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=24.8908296943231, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24645909, Length=244, Percent_Identity=48.7704918032787, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=26.7734553775744, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=28.6343612334802, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=28.6343612334802, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44208; Mature: 44208

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKIPSVGESVFEALVATWLRQDGDAVRKDEPVCEIETDKITMELNAEADGVLSIAVPA
CEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEECCCCCCEEEEEECC
GTTVKIGTVIGTIREGAAAPVAESPAPAQAAAAAPAAEPPLSPSVRKMARERGISPEAVP
CCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCC
GTGRGGRVTVDDLFSFAEKREQGASPAGAPPPAAPQPPAQQPRPAAEQPRQAEPPEADRT
CCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TRTPMTPIRKRIAERLMAARQQTAMLTTFNEADLGRIVELRARHKEQFAKRHGVSLGFMS
CCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FFVKACVEALKAFPLVNARIDGNDIVRHHYYNIGIAIGADKGLVVPVLRDADRLHFWEIE
HHHHHHHHHHHHCCCEEEEECCCHHHEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHH
QAIAAFVEKIKTNRLELSDLEGGTFSITNGGVYGSLLSTPILNPPQSGVLGMHAIQDRPV
HHHHHHHHHHHCCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCCCHHHHCCCCC
ARDGQVVIRPMMYLALSYDHRIIDGREAVGFLRTVKEYVEDPEELFLEG
CCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MEIKIPSVGESVFEALVATWLRQDGDAVRKDEPVCEIETDKITMELNAEADGVLSIAVPA
CEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEECCCCCCEEEEEECC
GTTVKIGTVIGTIREGAAAPVAESPAPAQAAAAAPAAEPPLSPSVRKMARERGISPEAVP
CCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCC
GTGRGGRVTVDDLFSFAEKREQGASPAGAPPPAAPQPPAQQPRPAAEQPRQAEPPEADRT
CCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TRTPMTPIRKRIAERLMAARQQTAMLTTFNEADLGRIVELRARHKEQFAKRHGVSLGFMS
CCCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FFVKACVEALKAFPLVNARIDGNDIVRHHYYNIGIAIGADKGLVVPVLRDADRLHFWEIE
HHHHHHHHHHHHCCCEEEEECCCHHHEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHH
QAIAAFVEKIKTNRLELSDLEGGTFSITNGGVYGSLLSTPILNPPQSGVLGMHAIQDRPV
HHHHHHHHHHHCCCEEEEECCCCEEEEECCCEEHHHHHCCCCCCCCCCCCCHHHHCCCCC
ARDGQVVIRPMMYLALSYDHRIIDGREAVGFLRTVKEYVEDPEELFLEG
CCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA