Click here to switch to the map view.

The map label for this gene is livJ [C]

Identifier: 39997484

GI number: 39997484

Start: 2619301

End: 2620449

Strand: Reverse

Name: livJ [C]

Synonym: GSU2389

Alternate gene names: 39997484

Gene position: 2620449-2619301 (Counterclockwise)

Preceding gene: 39997486

Following gene: 39997483

Centisome position: 68.7

GC content: 65.36

Gene sequence:

>1149_bases
ATGGTAATCCGGCCCTTCGCGCGGCTTTTCGCCCTGCGTCCGGTTGCGTTGCTCGCCTGCGCGGCTGTCATGGTTTCGAC
CATGTCGGGATGCCGACGCGACGACTCTTTGAAAATCGGCTATCTGGGCACCCTCAGTGGCCGCCATTCCGATCTGGGCG
TTGCCGGCCGCGACGGTACCATCTTTGCCGTGGAGGAGATCAACCGGGCGGGCGGCATTAACGGAAAGCGCCTGGAACTG
GTGATCCGCGACGACGAGGGCAAGGCCGGGAGCGCCCAGACTGCCGTGCGCGAGCTGATCGCGGCCGGCGTCGCCGCCAT
CGTCGGACCCATGACCAGCTCCATGGCCATGGCAACGGTGCCCGTCATTAACGGTTCGCCGGTGGTCATGATCAGCCCCA
CCGTCAGCACGGGCGATCTCAGCGGCAAAGACGACAACTTCCTCCGCATTTATCCCACCAACGCCCAGAAGACCCGGCAG
CTGGCCGAATATGCCCGCACGAGCCTGGGGCTGTCCCGGCTGGCCGTGGTGTACGACCTTTCCAACCGCTCCTACACCGA
CGACTGGCGGCAGGCGTTCACCCGGCAGTTCGAGTCGCTGGGCGGCACCGTCGCGCCGGCGGTCTCCTTTGATGCGTCCG
GCCAGACCGATTACCTGTCCGTGGCCAGAACGCTCCTGGCAAAGAAGCCGCAGGGGGTTCTCATCCTGGCCGGTGCCGTT
GATGCCGCCATGCTCTGCCAGCAGATCCGCAAGCTCGACACTTCCACGGCCCTCTTCGCCACCGAATGGTCCAGCACGCC
CGAGCTCCTCATGCACGGCGGGACCGCGGTGGAGGGGATCGTTTACTGCCAGAATTTCATCCGCGATGACGACTCCACCC
CCTATGTGACCTTCTGCCGGGCCTTCGAGACACGTTTCGGCCAGCTTCCCGACTTCGGGGCCGTCTATGCGTACCAGGCG
GTCAAGGTGACCGGCGCGGGGCTGGCAAAAAATCCCGCGCCCCGGGGGCTGAAGGGGGCGATCCTCGGCACCGGAACCTT
TCCCGGTCTTCAGGGCGAGTTTACCATTGATCGTTTCGGCGATACCGACCGGAAGCCGTTCCTGATGACCGTGCGCCAGG
GAGCGTTCCGGCGGGTGGAGGTGCCGTGA

Upstream 100 bases:

>100_bases
GCTGTGCGACGATGGAACATTGTTTGCAAGAGGTCTGGAGCTGTCCGATTCGGATGGGCATGATCACGTGAGAATATTCG
ATCACCGAGGAGCGATACCC

Downstream 100 bases:

>100_bases
ACCGGCGGATAAGCTTCAAGACGGCTCTGCTGGCGGCCCTGCTCATAGTAGGGGTGGTGCCGGTGCTCACTGTGGGACTG
ATCGCGCGATCAGCCCAGCG

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MVIRPFARLFALRPVALLACAAVMVSTMSGCRRDDSLKIGYLGTLSGRHSDLGVAGRDGTIFAVEEINRAGGINGKRLEL
VIRDDEGKAGSAQTAVRELIAAGVAAIVGPMTSSMAMATVPVINGSPVVMISPTVSTGDLSGKDDNFLRIYPTNAQKTRQ
LAEYARTSLGLSRLAVVYDLSNRSYTDDWRQAFTRQFESLGGTVAPAVSFDASGQTDYLSVARTLLAKKPQGVLILAGAV
DAAMLCQQIRKLDTSTALFATEWSSTPELLMHGGTAVEGIVYCQNFIRDDDSTPYVTFCRAFETRFGQLPDFGAVYAYQA
VKVTGAGLAKNPAPRGLKGAILGTGTFPGLQGEFTIDRFGDTDRKPFLMTVRQGAFRRVEVP

Sequences:

>Translated_382_residues
MVIRPFARLFALRPVALLACAAVMVSTMSGCRRDDSLKIGYLGTLSGRHSDLGVAGRDGTIFAVEEINRAGGINGKRLEL
VIRDDEGKAGSAQTAVRELIAAGVAAIVGPMTSSMAMATVPVINGSPVVMISPTVSTGDLSGKDDNFLRIYPTNAQKTRQ
LAEYARTSLGLSRLAVVYDLSNRSYTDDWRQAFTRQFESLGGTVAPAVSFDASGQTDYLSVARTLLAKKPQGVLILAGAV
DAAMLCQQIRKLDTSTALFATEWSSTPELLMHGGTAVEGIVYCQNFIRDDDSTPYVTFCRAFETRFGQLPDFGAVYAYQA
VKVTGAGLAKNPAPRGLKGAILGTGTFPGLQGEFTIDRFGDTDRKPFLMTVRQGAFRRVEVP
>Mature_382_residues
MVIRPFARLFALRPVALLACAAVMVSTMSGCRRDDSLKIGYLGTLSGRHSDLGVAGRDGTIFAVEEINRAGGINGKRLEL
VIRDDEGKAGSAQTAVRELIAAGVAAIVGPMTSSMAMATVPVINGSPVVMISPTVSTGDLSGKDDNFLRIYPTNAQKTRQ
LAEYARTSLGLSRLAVVYDLSNRSYTDDWRQAFTRQFESLGGTVAPAVSFDASGQTDYLSVARTLLAKKPQGVLILAGAV
DAAMLCQQIRKLDTSTALFATEWSSTPELLMHGGTAVEGIVYCQNFIRDDDSTPYVTFCRAFETRFGQLPDFGAVYAYQA
VKVTGAGLAKNPAPRGLKGAILGTGTFPGLQGEFTIDRFGDTDRKPFLMTVRQGAFRRVEVP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=359, Percent_Identity=23.3983286908078, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40900; Mature: 40900

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIRPFARLFALRPVALLACAAVMVSTMSGCRRDDSLKIGYLGTLSGRHSDLGVAGRDGT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEECCCCC
IFAVEEINRAGGINGKRLELVIRDDEGKAGSAQTAVRELIAAGVAAIVGPMTSSMAMATV
EEEEEHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PVINGSPVVMISPTVSTGDLSGKDDNFLRIYPTNAQKTRQLAEYARTSLGLSRLAVVYDL
EEECCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHEEEEEEC
SNRSYTDDWRQAFTRQFESLGGTVAPAVSFDASGQTDYLSVARTLLAKKPQGVLILAGAV
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHH
DAAMLCQQIRKLDTSTALFATEWSSTPELLMHGGTAVEGIVYCQNFIRDDDSTPYVTFCR
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
AFETRFGQLPDFGAVYAYQAVKVTGAGLAKNPAPRGLKGAILGTGTFPGLQGEFTIDRFG
HHHHHHCCCCCCCHHEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCC
DTDRKPFLMTVRQGAFRRVEVP
CCCCCCEEEEECCCCCEEECCC
>Mature Secondary Structure
MVIRPFARLFALRPVALLACAAVMVSTMSGCRRDDSLKIGYLGTLSGRHSDLGVAGRDGT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCEECCCCC
IFAVEEINRAGGINGKRLELVIRDDEGKAGSAQTAVRELIAAGVAAIVGPMTSSMAMATV
EEEEEHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
PVINGSPVVMISPTVSTGDLSGKDDNFLRIYPTNAQKTRQLAEYARTSLGLSRLAVVYDL
EEECCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHEEEEEEC
SNRSYTDDWRQAFTRQFESLGGTVAPAVSFDASGQTDYLSVARTLLAKKPQGVLILAGAV
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHH
DAAMLCQQIRKLDTSTALFATEWSSTPELLMHGGTAVEGIVYCQNFIRDDDSTPYVTFCR
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
AFETRFGQLPDFGAVYAYQAVKVTGAGLAKNPAPRGLKGAILGTGTFPGLQGEFTIDRFG
HHHHHHCCCCCCCHHEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCC
DTDRKPFLMTVRQGAFRRVEVP
CCCCCCEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]