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The map label for this gene is kefA [H]

Identifier: 39997414

GI number: 39997414

Start: 2535599

End: 2536474

Strand: Reverse

Name: kefA [H]

Synonym: GSU2316

Alternate gene names: 39997414

Gene position: 2536474-2535599 (Counterclockwise)

Preceding gene: 39997415

Following gene: 39997413

Centisome position: 66.5

GC content: 57.53

Gene sequence:

>876_bases
ATGGAAACCTTGATGGAACTGATTGGAACAGTCAGGCAATACCTTGAAATTCCCATTCTGAAGCTCGGCGGCGCCCCCGT
GACCCTCTGGGCCATCATTCAACTGGTGGTGCTGGTGGCGCTTCTCTTCTACCTCTCGGGCAAGCTGCGGACCTGGATCG
TTGAGCAGTTCCTCACCCGGACCAGGATGGAGCTGGGCGCCCGCCAGGCCACCGGCTCCATTATTCGCTACACCATCATA
GCCATCGGTTTCATCGTCATCCTCCAGACCGCCGGCATCGACCTTACCGCACTCAACGTTCTGGCCGGCGCCGTCGGCAT
CGGCGTCGGTTTCGGCCTCCAGAACATCGTCAATAACTTCGTCAGCGGCATCATCATCCTCTTCGAGCGTCCGATCAAGG
TGGGGGACCGGATCGTGGTGGGCACCGTTGAGGGGGATGTGGTTCATATCGGCGGCAGGAGCACGACCGTGGTGACTAAC
GACAACATCACCATCATTGTGCCCAATTCCAAGTTCATCACCGAGAATGTGGTGAACTGGAGCCACAACGAGCGGAAGGT
CCGGTTCCGGATTCCCGTGAGCGTTGCCTACGGCAGCGATGTTCATCTGGTGGAGCGGCTACTCCTGGAGGTGGCTGCCG
ACAATGCCGACGTGCTGGAAAAGCCGCCACCTGCCGTGCGTCTCATGGAATTCGGGGACAGCGGACTCAACTTCGAGTTG
AGGGCCTGGAGCACTACTCTCATCCATCGGCGCGGACTTCTTACGAGCGCCCTCAATTACGGCATTTACAAGGCGTTCAC
AGAAAACGGCATTGAAATACCATTTCCCCGGCGCGATATTCGCATATTTAATGACGGGATGGGCAACCCATCATAA

Upstream 100 bases:

>100_bases
TGATTTTCTGCGAGTGGGAACGCCACTCAATATTCTTTTTTGGGTTCTGGCGACGATATTCATTCCGCGCTTCTGGCCTT
TTTGACGGGGAGAGCTGCCC

Downstream 100 bases:

>100_bases
TGATTGTGGATTTGAGTTGAGCAATTGCCGTGCTGAGGGGGATTCATGATCACGCAGATCAAAAAATTTGAAAAAATGAT
TATTTTCTCACTGGTAGTCA

Product: mechanosensitive ion channel family protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
METLMELIGTVRQYLEIPILKLGGAPVTLWAIIQLVVLVALLFYLSGKLRTWIVEQFLTRTRMELGARQATGSIIRYTII
AIGFIVILQTAGIDLTALNVLAGAVGIGVGFGLQNIVNNFVSGIIILFERPIKVGDRIVVGTVEGDVVHIGGRSTTVVTN
DNITIIVPNSKFITENVVNWSHNERKVRFRIPVSVAYGSDVHLVERLLLEVAADNADVLEKPPPAVRLMEFGDSGLNFEL
RAWSTTLIHRRGLLTSALNYGIYKAFTENGIEIPFPRRDIRIFNDGMGNPS

Sequences:

>Translated_291_residues
METLMELIGTVRQYLEIPILKLGGAPVTLWAIIQLVVLVALLFYLSGKLRTWIVEQFLTRTRMELGARQATGSIIRYTII
AIGFIVILQTAGIDLTALNVLAGAVGIGVGFGLQNIVNNFVSGIIILFERPIKVGDRIVVGTVEGDVVHIGGRSTTVVTN
DNITIIVPNSKFITENVVNWSHNERKVRFRIPVSVAYGSDVHLVERLLLEVAADNADVLEKPPPAVRLMEFGDSGLNFEL
RAWSTTLIHRRGLLTSALNYGIYKAFTENGIEIPFPRRDIRIFNDGMGNPS
>Mature_291_residues
METLMELIGTVRQYLEIPILKLGGAPVTLWAIIQLVVLVALLFYLSGKLRTWIVEQFLTRTRMELGARQATGSIIRYTII
AIGFIVILQTAGIDLTALNVLAGAVGIGVGFGLQNIVNNFVSGIIILFERPIKVGDRIVVGTVEGDVVHIGGRSTTVVTN
DNITIIVPNSKFITENVVNWSHNERKVRFRIPVSVAYGSDVHLVERLLLEVAADNADVLEKPPPAVRLMEFGDSGLNFEL
RAWSTTLIHRRGLLTSALNYGIYKAFTENGIEIPFPRRDIRIFNDGMGNPS

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=257, Percent_Identity=38.5214007782101, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI2367355, Length=257, Percent_Identity=32.6848249027237, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789291, Length=227, Percent_Identity=29.0748898678414, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787591, Length=253, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32076; Mature: 32076

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLMELIGTVRQYLEIPILKLGGAPVTLWAIIQLVVLVALLFYLSGKLRTWIVEQFLTR
CHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TRMELGARQATGSIIRYTIIAIGFIVILQTAGIDLTALNVLAGAVGIGVGFGLQNIVNNF
HHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VSGIIILFERPIKVGDRIVVGTVEGDVVHIGGRSTTVVTNDNITIIVPNSKFITENVVNW
HHHEEEEEECCCCCCCEEEEEEECCCEEEECCCEEEEEECCCEEEEECCCCCHHHHHHCC
SHNERKVRFRIPVSVAYGSDVHLVERLLLEVAADNADVLEKPPPAVRLMEFGDSGLNFEL
CCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCCCCEEE
RAWSTTLIHRRGLLTSALNYGIYKAFTENGIEIPFPRRDIRIFNDGMGNPS
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
METLMELIGTVRQYLEIPILKLGGAPVTLWAIIQLVVLVALLFYLSGKLRTWIVEQFLTR
CHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TRMELGARQATGSIIRYTIIAIGFIVILQTAGIDLTALNVLAGAVGIGVGFGLQNIVNNF
HHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VSGIIILFERPIKVGDRIVVGTVEGDVVHIGGRSTTVVTNDNITIIVPNSKFITENVVNW
HHHEEEEEECCCCCCCEEEEEEECCCEEEECCCEEEEEECCCEEEEECCCCCHHHHHHCC
SHNERKVRFRIPVSVAYGSDVHLVERLLLEVAADNADVLEKPPPAVRLMEFGDSGLNFEL
CCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCCCCEEE
RAWSTTLIHRRGLLTSALNYGIYKAFTENGIEIPFPRRDIRIFNDGMGNPS
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]