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The map label for this gene is cutE

Identifier: 39997379

GI number: 39997379

Start: 2497042

End: 2498475

Strand: Reverse

Name: cutE

Synonym: GSU2281

Alternate gene names: 39997379

Gene position: 2498475-2497042 (Counterclockwise)

Preceding gene: 308513314

Following gene: 39997378

Centisome position: 65.51

GC content: 61.72

Gene sequence:

>1434_bases
ATGCCCGGCTTCAATGCCGTCCCCCGGCGCGACTACGTCATGGCTGCCCTTTCGGGCGTTCTCCTGGCCTTGTCGTTCCC
CAAGCCCGGCTTTTCCATCTTGGCGTGGGTGGCCCTGGTGCCGTTACTGCTCGCCTGCGGCCGCAAGGCTCCCGCCGTCG
CCTTCCGGCTCGGCTTCACCGCGGGATTGGTGGCCTACGCCGGCATTCTCTACTGGATCAATATTGCCGTGGTAACCTAC
GGCCGTCTCCACTGGAGCGTCAGCATCGTCATCTTTCTGATGCTGGCGGGCTATCTGGCCCTCTATCCCGCCGCAACCGC
CTATGTCGTCAGACGCGGCGAAGATCGGGGAATCTCCGCCCTTCTTGCGTTTCCGGTGGTATGGGTGGGGCTGGAATATA
TCAGGTCATTTCTCCTCACCGGTTTTCCCTGGGCAAGCCTCGGGTACTCCCAGTACCGGACCCTGCCGCTCATCCAGATA
GCGGATCTGACCGGCGTCTACGGCCTTTCGTTTCTCATCGCCCTGAGCAATGTGGTTCTGTACCGGATCATCCGCGGTTT
TGCGGCCCGCGAACGGGCGCCCTATCCGGTGAAGAGCGCAGCTATCCTGGTTCTGCTTCTGGTCGCCACCCTTGCTTACG
GTTTCAATCGCCTGCACCGCCCCGAGGCGGGGGCTCCTTTCTCCGTAGCCCTGATTCAGGGCAATATCGACCAGAGCGTA
AAGTGGGACCCGGCTTTTCAGGAGGCGACGGTGGCGGTGTACGAGCGTCTGTCCCGCAAGGCGTGCAGCACCGGCCCCGC
CGACCTGGTGGTCTGGCCCGAGAGCGCGGTGCCATTTTATCTTCAGAACGAGGAGAAATACGCGTCCCGCATCCGGAATC
TGACGAGGGAGCTGCGCAGTTGCACAGTTGTGGGGAGCCCGGCCTTTGAACGGGACGGCGAGACGATTCGCTACCTGAAC
AGTGCGTTCCTTCTGTCCCCCTGGGGGGATGTTGTGGGCCGCAGCGACAAGATCCACCTGGTCCCCTTCGGCGAGTACGT
GCCCATGGCGAAGTTTCTCCCCTTCGTCAACAAGATCGTGGCCGGCATCGGCGACTTTTCGCCCGGAGCCAGGATTGCCT
CCCTTGAGACGGGGAAGGGAGCCATCGGGGTGCTGGTCTGCTTCGAGGGAATATTCCCCGAGCTGGCGCGGGGGTATGTG
CGGGCCGGAAGCAGGGTTCTGGTCAACATAACCAACGACGCCTGGTACAAGCGATCCTCCGCTCCTTACCAGCATCTCTC
CATGACAGTCTTTCGAGCCGTGGAGAACCGGGTTCCCCTGGTGCGGGCCGCCAACACCGGCATCACTGCCATCATCGACA
GCAAAGGACACATTCTCAGAATGACCAATCTGTTCGAGGTATGGCGGCCCCGGTTTTCTTGGACACGAAAATGA

Upstream 100 bases:

>100_bases
TCCTGGAGGCCGGCGAACGTAACATTAAGAAAGTACGGATCGCCCGCCGCAGTGACGAGGCCGGGGAGGCCCAATCGTAG
TGGATTACCGCAGATTTCAC

Downstream 100 bases:

>100_bases
GGTAAATCTAGGTGTTCAAGGAGGTCGTCAATGGAAAAGGATGTCAGTGTCTCCGGTGCGGCGGAAGGAGAGCGTAGCTC
GACTGGAGCAGCACCGGAAG

Product: apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ALP N-acyltransferase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPGFNAVPRRDYVMAALSGVLLALSFPKPGFSILAWVALVPLLLACGRKAPAVAFRLGFTAGLVAYAGILYWINIAVVTY
GRLHWSVSIVIFLMLAGYLALYPAATAYVVRRGEDRGISALLAFPVVWVGLEYIRSFLLTGFPWASLGYSQYRTLPLIQI
ADLTGVYGLSFLIALSNVVLYRIIRGFAARERAPYPVKSAAILVLLLVATLAYGFNRLHRPEAGAPFSVALIQGNIDQSV
KWDPAFQEATVAVYERLSRKACSTGPADLVVWPESAVPFYLQNEEKYASRIRNLTRELRSCTVVGSPAFERDGETIRYLN
SAFLLSPWGDVVGRSDKIHLVPFGEYVPMAKFLPFVNKIVAGIGDFSPGARIASLETGKGAIGVLVCFEGIFPELARGYV
RAGSRVLVNITNDAWYKRSSAPYQHLSMTVFRAVENRVPLVRAANTGITAIIDSKGHILRMTNLFEVWRPRFSWTRK

Sequences:

>Translated_477_residues
MPGFNAVPRRDYVMAALSGVLLALSFPKPGFSILAWVALVPLLLACGRKAPAVAFRLGFTAGLVAYAGILYWINIAVVTY
GRLHWSVSIVIFLMLAGYLALYPAATAYVVRRGEDRGISALLAFPVVWVGLEYIRSFLLTGFPWASLGYSQYRTLPLIQI
ADLTGVYGLSFLIALSNVVLYRIIRGFAARERAPYPVKSAAILVLLLVATLAYGFNRLHRPEAGAPFSVALIQGNIDQSV
KWDPAFQEATVAVYERLSRKACSTGPADLVVWPESAVPFYLQNEEKYASRIRNLTRELRSCTVVGSPAFERDGETIRYLN
SAFLLSPWGDVVGRSDKIHLVPFGEYVPMAKFLPFVNKIVAGIGDFSPGARIASLETGKGAIGVLVCFEGIFPELARGYV
RAGSRVLVNITNDAWYKRSSAPYQHLSMTVFRAVENRVPLVRAANTGITAIIDSKGHILRMTNLFEVWRPRFSWTRK
>Mature_476_residues
PGFNAVPRRDYVMAALSGVLLALSFPKPGFSILAWVALVPLLLACGRKAPAVAFRLGFTAGLVAYAGILYWINIAVVTYG
RLHWSVSIVIFLMLAGYLALYPAATAYVVRRGEDRGISALLAFPVVWVGLEYIRSFLLTGFPWASLGYSQYRTLPLIQIA
DLTGVYGLSFLIALSNVVLYRIIRGFAARERAPYPVKSAAILVLLLVATLAYGFNRLHRPEAGAPFSVALIQGNIDQSVK
WDPAFQEATVAVYERLSRKACSTGPADLVVWPESAVPFYLQNEEKYASRIRNLTRELRSCTVVGSPAFERDGETIRYLNS
AFLLSPWGDVVGRSDKIHLVPFGEYVPMAKFLPFVNKIVAGIGDFSPGARIASLETGKGAIGVLVCFEGIFPELARGYVR
AGSRVLVNITNDAWYKRSSAPYQHLSMTVFRAVENRVPLVRAANTGITAIIDSKGHILRMTNLFEVWRPRFSWTRK

Specific function: Transfers the fatty acyl group on membrane lipoproteins

COG id: COG0815

COG function: function code M; Apolipoprotein N-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Escherichia coli, GI1786878, Length=467, Percent_Identity=29.5503211991435, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LNT_GEOSL (P61035)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953330.1
- ProteinModelPortal:   P61035
- GeneID:   2686662
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2281
- NMPDR:   fig|243231.1.peg.2268
- TIGR:   GSU2281
- HOGENOM:   HBG616234
- OMA:   KGHIRGM
- ProtClustDB:   PRK00302
- BioCyc:   GSUL243231:GSU_2281-MONOMER
- HAMAP:   MF_01148
- InterPro:   IPR004563
- InterPro:   IPR003010
- Gene3D:   G3DSA:3.60.110.10
- TIGRFAMs:   TIGR00546

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 2.3.1.- [C]

Molecular weight: Translated: 52609; Mature: 52478

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xeef877c)-; HASH(0x103ce4cc)-; HASH(0xddacc18)-; HASH(0xfee0e04)-; HASH(0x10501a88)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGFNAVPRRDYVMAALSGVLLALSFPKPGFSILAWVALVPLLLACGRKAPAVAFRLGFT
CCCCCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHEEHHHHH
AGLVAYAGILYWINIAVVTYGRLHWSVSIVIFLMLAGYLALYPAATAYVVRRGEDRGISA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLAFPVVWVGLEYIRSFLLTGFPWASLGYSQYRTLPLIQIADLTGVYGLSFLIALSNVVL
HHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
YRIIRGFAARERAPYPVKSAAILVLLLVATLAYGFNRLHRPEAGAPFSVALIQGNIDQSV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
KWDPAFQEATVAVYERLSRKACSTGPADLVVWPESAVPFYLQNEEKYASRIRNLTRELRS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHH
CTVVGSPAFERDGETIRYLNSAFLLSPWGDVVGRSDKIHLVPFGEYVPMAKFLPFVNKIV
EEEECCCCCCCCCCHHHHHCCHHEECCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGIGDFSPGARIASLETGKGAIGVLVCFEGIFPELARGYVRAGSRVLVNITNDAWYKRSS
HHCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCC
APYQHLSMTVFRAVENRVPLVRAANTGITAIIDSKGHILRMTNLFEVWRPRFSWTRK
CCHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCEEEHHHHHHHHCCCCCCCCH
>Mature Secondary Structure 
PGFNAVPRRDYVMAALSGVLLALSFPKPGFSILAWVALVPLLLACGRKAPAVAFRLGFT
CCCCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHEEHHHHH
AGLVAYAGILYWINIAVVTYGRLHWSVSIVIFLMLAGYLALYPAATAYVVRRGEDRGISA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLAFPVVWVGLEYIRSFLLTGFPWASLGYSQYRTLPLIQIADLTGVYGLSFLIALSNVVL
HHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
YRIIRGFAARERAPYPVKSAAILVLLLVATLAYGFNRLHRPEAGAPFSVALIQGNIDQSV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
KWDPAFQEATVAVYERLSRKACSTGPADLVVWPESAVPFYLQNEEKYASRIRNLTRELRS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHH
CTVVGSPAFERDGETIRYLNSAFLLSPWGDVVGRSDKIHLVPFGEYVPMAKFLPFVNKIV
EEEECCCCCCCCCCHHHHHCCHHEECCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGIGDFSPGARIASLETGKGAIGVLVCFEGIFPELARGYVRAGSRVLVNITNDAWYKRSS
HHCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCC
APYQHLSMTVFRAVENRVPLVRAANTGITAIIDSKGHILRMTNLFEVWRPRFSWTRK
CCHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCEEEHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA