Click here to switch to the map view.

The map label for this gene is rfaF [H]

Identifier: 39997354

GI number: 39997354

Start: 2471341

End: 2472411

Strand: Reverse

Name: rfaF [H]

Synonym: GSU2256

Alternate gene names: 39997354

Gene position: 2472411-2471341 (Counterclockwise)

Preceding gene: 39997355

Following gene: 39997353

Centisome position: 64.82

GC content: 64.43

Gene sequence:

>1071_bases
TTGGATATTCAGCAGGAGACAATCAGCAGCATACTCGTTCGGTCCACCAACTGGATCGGCGATGCGGTGATGACCACACC
GGCGCTGCGGGCAATTCGCGAGAGCTTCCCGAACGCCCGGGTGGCGGTATTGGCCAATCCGCTGGTGGCGCCGCTGTTCA
CCGTTCACGGGGCCGTGGACGAGGTCATCATCTATGACCGAAAAGGTGTGCATGCCGGCCTGCGAGGCAAACTCCGGCTT
GCCCGGGAACTGAGAGCCCGGCGGTTCGATCTGGCGATTCTGCTGCAGAATGCCGTTGATGCGGCACTTATTGCCTGGCT
TGCGCGGATTCCCCGGCGCATGGGATACCGGACCGATGGCCGCGGACCGCTTCTGACCCACGGAGTTGCGGTTGACGGCG
CCACGCGCCTGCTCCATCACGTGGAATACTACCTGGCCATGCTCGCCCGTTTCGGGGTCACCACCAGTGATCGGCATCTT
GAACTGGCCACAACGGCCGACGAGGATGCCGCCATGGCAGAACTTTTGGCGGGGCACGGAATCGGGACTCGCGATGCCCT
CCTTGGCATTAACCCCGGCGCCACGTACGGCGCCGCCAAACGGTGGTATCCGGAGCGTTTTGCCGCCGTGGCCGACGAGT
TGGCCAGCCGCTGGAGTGCCAGGGTTGTGGTGACCGGCGGGCCGGGTGAAGCGGCCATTGCCGCCGATATTGCCGCGGCC
ATGACCACCCCCTGCGTCGTCATGGCGGGAAAGACATCGGTGCGGGAACTCATGGCGCTCATCAAGCGCTGTGAGTTCTT
TATCACCAATGATTCGGGCCCCATGCACATTGCGGCGGCCTTCGGCGTCCCGCTGGTGGCCGTGTTCGGCTCCACGGACC
ATTCGACCACCTCCCCCTGGAGCGAACGGGCCGTTATCGTGCGTCGCGACACCGACTGCGCTCCGTGTCTGCTGCGGGAA
TGCCCCACCGACCACCGATGCATGACCGCGGTGACGACAGCCGAGGTGGTGGAGGCCGCAGACCGGCTTCGGGCAGGTCG
ACAAGCACTATCTTCCCAGGGAGTATCGTGA

Upstream 100 bases:

>100_bases
CGAGGCATGCCGCCTCAGGTTCCCCGTGCGGGACGATATCCCTGTGATGCTCCTCGACGAGGCTGAACGCATCGGGGACC
GGTAAACGGGGCTACACGCT

Downstream 100 bases:

>100_bases
TGGCCAGACCCAGGCTGTATATCGATGAATTCGTCGGCATCACCAATCGACTCGAAACTCTGCCGCTCGCCTTTGCCATC
CAGAAGGAATACGGGCACGA

Product: ADP-heptose--LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDIQQETISSILVRSTNWIGDAVMTTPALRAIRESFPNARVAVLANPLVAPLFTVHGAVDEVIIYDRKGVHAGLRGKLRL
ARELRARRFDLAILLQNAVDAALIAWLARIPRRMGYRTDGRGPLLTHGVAVDGATRLLHHVEYYLAMLARFGVTTSDRHL
ELATTADEDAAMAELLAGHGIGTRDALLGINPGATYGAAKRWYPERFAAVADELASRWSARVVVTGGPGEAAIAADIAAA
MTTPCVVMAGKTSVRELMALIKRCEFFITNDSGPMHIAAAFGVPLVAVFGSTDHSTTSPWSERAVIVRRDTDCAPCLLRE
CPTDHRCMTAVTTAEVVEAADRLRAGRQALSSQGVS

Sequences:

>Translated_356_residues
MDIQQETISSILVRSTNWIGDAVMTTPALRAIRESFPNARVAVLANPLVAPLFTVHGAVDEVIIYDRKGVHAGLRGKLRL
ARELRARRFDLAILLQNAVDAALIAWLARIPRRMGYRTDGRGPLLTHGVAVDGATRLLHHVEYYLAMLARFGVTTSDRHL
ELATTADEDAAMAELLAGHGIGTRDALLGINPGATYGAAKRWYPERFAAVADELASRWSARVVVTGGPGEAAIAADIAAA
MTTPCVVMAGKTSVRELMALIKRCEFFITNDSGPMHIAAAFGVPLVAVFGSTDHSTTSPWSERAVIVRRDTDCAPCLLRE
CPTDHRCMTAVTTAEVVEAADRLRAGRQALSSQGVS
>Mature_356_residues
MDIQQETISSILVRSTNWIGDAVMTTPALRAIRESFPNARVAVLANPLVAPLFTVHGAVDEVIIYDRKGVHAGLRGKLRL
ARELRARRFDLAILLQNAVDAALIAWLARIPRRMGYRTDGRGPLLTHGVAVDGATRLLHHVEYYLAMLARFGVTTSDRHL
ELATTADEDAAMAELLAGHGIGTRDALLGINPGATYGAAKRWYPERFAAVADELASRWSARVVVTGGPGEAAIAADIAAA
MTTPCVVMAGKTSVRELMALIKRCEFFITNDSGPMHIAAAFGVPLVAVFGSTDHSTTSPWSERAVIVRRDTDCAPCLLRE
CPTDHRCMTAVTTAEVVEAADRLRAGRQALSSQGVS

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=310, Percent_Identity=29.6774193548387, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790062, Length=337, Percent_Identity=28.7833827893175, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38302; Mature: 38302

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQQETISSILVRSTNWIGDAVMTTPALRAIRESFPNARVAVLANPLVAPLFTVHGAVD
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCHH
EVIIYDRKGVHAGLRGKLRLARELRARRFDLAILLQNAVDAALIAWLARIPRRMGYRTDG
HEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RGPLLTHGVAVDGATRLLHHVEYYLAMLARFGVTTSDRHLELATTADEDAAMAELLAGHG
CCCEEECCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCC
IGTRDALLGINPGATYGAAKRWYPERFAAVADELASRWSARVVVTGGPGEAAIAADIAAA
CCCCCCEEECCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
MTTPCVVMAGKTSVRELMALIKRCEFFITNDSGPMHIAAAFGVPLVAVFGSTDHSTTSPW
HHCCEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEHHCCEEEEEECCCCCCCCCCC
SERAVIVRRDTDCAPCLLRECPTDHRCMTAVTTAEVVEAADRLRAGRQALSSQGVS
CCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDIQQETISSILVRSTNWIGDAVMTTPALRAIRESFPNARVAVLANPLVAPLFTVHGAVD
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCHH
EVIIYDRKGVHAGLRGKLRLARELRARRFDLAILLQNAVDAALIAWLARIPRRMGYRTDG
HEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RGPLLTHGVAVDGATRLLHHVEYYLAMLARFGVTTSDRHLELATTADEDAAMAELLAGHG
CCCEEECCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCC
IGTRDALLGINPGATYGAAKRWYPERFAAVADELASRWSARVVVTGGPGEAAIAADIAAA
CCCCCCEEECCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
MTTPCVVMAGKTSVRELMALIKRCEFFITNDSGPMHIAAAFGVPLVAVFGSTDHSTTSPW
HHCCEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEHHCCEEEEEECCCCCCCCCCC
SERAVIVRRDTDCAPCLLRECPTDHRCMTAVTTAEVVEAADRLRAGRQALSSQGVS
CCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]