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The map label for this gene is cca [H]

Identifier: 39997282

GI number: 39997282

Start: 2393737

End: 2395029

Strand: Reverse

Name: cca [H]

Synonym: GSU2184

Alternate gene names: 39997282

Gene position: 2395029-2393737 (Counterclockwise)

Preceding gene: 39997283

Following gene: 39997278

Centisome position: 62.79

GC content: 67.36

Gene sequence:

>1293_bases
ATGGACCACCGCCTCCTTTCGTTCATTTCCGCGCCATTGCCCTCCCTTATTGCATCCCTTGCCCGTCACGGAGGCTTCGG
TGCGTGGTTTGTGGGTGGGTGCGTCCGCGATGCCCTGCTGGCGAGGCCATCCAATGACATCGACATCGTGGTCGGCCCCG
GCGGGGAAGACCTGCCCCGGGCCGTTGCCGCCCGGATCGGCGGCAGCTTCTTCCCGCTCGACGAAGAGCGGGGCCATGCC
CGCGTTGTACTGAAAGGGGAGGGCGCCAGCTGCGACTTCGCTCCCCTCCAGGGCGGAACCATCGCTGCCGACCTCGCCCT
GCGGGACTTCACCATCAACGCCCTTGCCGTGTCCTGTGGTTCGGACGACCTGCTGGACCCGCTGGGCGGAGCCGCCGACT
TGGCGCAGCGCGTCATCCGTGCCTGCTCTGCCGGGGCCTTTGCCGCCGATCCGCTCAGGATCGTGCGTGCCTACCGTTTT
GCCGCCCATCTCGACTTCGAGATCCACGCCGCAACCCTGGCGCTTATCCCCGATCATGCACCGCTCCTGGCCACCGTGGC
CGGAGAGCGGATTAGGGACGAACTCTTCCGGATGCTCGATCTGCCGCACGCAGTTCCCTATGTCCTTAAGATGTCCTGTG
CCGGCGTGACGGGCGCCATCTTCGGGGCCGACGACCTTCCCGCCGATACCGCCGCCGGCGCCCTTGACCGGGTCGAGTCC
CTCTGCCGCGATCTCTCGGCCTTCGGCACCGAGGCGGAGCCGGTTCGGGCACGGCTACGGCAGGAGGTCCAGCCCGGCAT
CACCATCCGCGCCCTGGCGAAACTCGCCGCATTCCTGAACGGCGCGGGCATACCTGCAGGCATCGCATCGCAACGGCTCA
TGCTCGGCAAGGCCGCCACCCGGCTTCTGGAACTCCTCTGCTCCTCGGCCCGCCTTACCTGGCCTGCCCCTGCCGCCGCG
CCGGACCCGCACGCCCTCTTTACTCTCTTCTGCCACCGCGAACCGGCCGGTTGCGAACAGCTCATCCTTCCCTTGGCGGA
AGGCATTCTGCCTGAGGACAGATGCCGCCACCTTGCCGCCTACCTGACACGCCAGCACATCCCCCGCGGCGGACGGCTCC
TCCTCACCGGCGACGACATCATGATCCTTCTCGGCTTGCCTCCGGGCAGACAGGTGGGGGAAGCCATTGAACTGCTCCGC
GCAGCCCAGAGCACCGGCGAAGTACGCACCCGCGCCGAGGCACAGCGCTATCTGGCCAAAAAACAGTTGACAACTCCCGA
GCCCCTGCGCTAA

Upstream 100 bases:

>100_bases
GGGTCACCGCCTGACGGACTCCGCACATTCGCTACGTGAAAGCCCGCCTTGCGCGGGCTTTTTTGTTGCCTCCGCCCCGG
TCGGGCGGTAAGCTCGAATC

Downstream 100 bases:

>100_bases
ATACATACCTCTCTATCGAGCGGGAATAACTCAGTGGTAGAGTGTCAGCTTCCCAAGCTGAAGGTCGCGGGTTCGAATCC
CGTTTCCCGCTCCAATAAAA

Product: polyA polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDHRLLSFISAPLPSLIASLARHGGFGAWFVGGCVRDALLARPSNDIDIVVGPGGEDLPRAVAARIGGSFFPLDEERGHA
RVVLKGEGASCDFAPLQGGTIAADLALRDFTINALAVSCGSDDLLDPLGGAADLAQRVIRACSAGAFAADPLRIVRAYRF
AAHLDFEIHAATLALIPDHAPLLATVAGERIRDELFRMLDLPHAVPYVLKMSCAGVTGAIFGADDLPADTAAGALDRVES
LCRDLSAFGTEAEPVRARLRQEVQPGITIRALAKLAAFLNGAGIPAGIASQRLMLGKAATRLLELLCSSARLTWPAPAAA
PDPHALFTLFCHREPAGCEQLILPLAEGILPEDRCRHLAAYLTRQHIPRGGRLLLTGDDIMILLGLPPGRQVGEAIELLR
AAQSTGEVRTRAEAQRYLAKKQLTTPEPLR

Sequences:

>Translated_430_residues
MDHRLLSFISAPLPSLIASLARHGGFGAWFVGGCVRDALLARPSNDIDIVVGPGGEDLPRAVAARIGGSFFPLDEERGHA
RVVLKGEGASCDFAPLQGGTIAADLALRDFTINALAVSCGSDDLLDPLGGAADLAQRVIRACSAGAFAADPLRIVRAYRF
AAHLDFEIHAATLALIPDHAPLLATVAGERIRDELFRMLDLPHAVPYVLKMSCAGVTGAIFGADDLPADTAAGALDRVES
LCRDLSAFGTEAEPVRARLRQEVQPGITIRALAKLAAFLNGAGIPAGIASQRLMLGKAATRLLELLCSSARLTWPAPAAA
PDPHALFTLFCHREPAGCEQLILPLAEGILPEDRCRHLAAYLTRQHIPRGGRLLLTGDDIMILLGLPPGRQVGEAIELLR
AAQSTGEVRTRAEAQRYLAKKQLTTPEPLR
>Mature_430_residues
MDHRLLSFISAPLPSLIASLARHGGFGAWFVGGCVRDALLARPSNDIDIVVGPGGEDLPRAVAARIGGSFFPLDEERGHA
RVVLKGEGASCDFAPLQGGTIAADLALRDFTINALAVSCGSDDLLDPLGGAADLAQRVIRACSAGAFAADPLRIVRAYRF
AAHLDFEIHAATLALIPDHAPLLATVAGERIRDELFRMLDLPHAVPYVLKMSCAGVTGAIFGADDLPADTAAGALDRVES
LCRDLSAFGTEAEPVRARLRQEVQPGITIRALAKLAAFLNGAGIPAGIASQRLMLGKAATRLLELLCSSARLTWPAPAAA
PDPHALFTLFCHREPAGCEQLILPLAEGILPEDRCRHLAAYLTRQHIPRGGRLLLTGDDIMILLGLPPGRQVGEAIELLR
AAQSTGEVRTRAEAQRYLAKKQLTTPEPLR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=182, Percent_Identity=39.5604395604396, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081691, Length=202, Percent_Identity=31.6831683168317, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45587; Mature: 45587

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHRLLSFISAPLPSLIASLARHGGFGAWFVGGCVRDALLARPSNDIDIVVGPGGEDLPR
CCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHH
AVAARIGGSFFPLDEERGHARVVLKGEGASCDFAPLQGGTIAADLALRDFTINALAVSCG
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHEEEEEEEEECC
SDDLLDPLGGAADLAQRVIRACSAGAFAADPLRIVRAYRFAAHLDFEIHAATLALIPDHA
CCCCHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCC
PLLATVAGERIRDELFRMLDLPHAVPYVLKMSCAGVTGAIFGADDLPADTAAGALDRVES
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHH
LCRDLSAFGTEAEPVRARLRQEVQPGITIRALAKLAAFLNGAGIPAGIASQRLMLGKAAT
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RLLELLCSSARLTWPAPAAAPDPHALFTLFCHREPAGCEQLILPLAEGILPEDRCRHLAA
HHHHHHHHCCCCCCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHH
YLTRQHIPRGGRLLLTGDDIMILLGLPPGRQVGEAIELLRAAQSTGEVRTRAEAQRYLAK
HHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQLTTPEPLR
HCCCCCCCCC
>Mature Secondary Structure
MDHRLLSFISAPLPSLIASLARHGGFGAWFVGGCVRDALLARPSNDIDIVVGPGGEDLPR
CCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHH
AVAARIGGSFFPLDEERGHARVVLKGEGASCDFAPLQGGTIAADLALRDFTINALAVSCG
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHEEEEEEEEECC
SDDLLDPLGGAADLAQRVIRACSAGAFAADPLRIVRAYRFAAHLDFEIHAATLALIPDHA
CCCCHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCC
PLLATVAGERIRDELFRMLDLPHAVPYVLKMSCAGVTGAIFGADDLPADTAAGALDRVES
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHH
LCRDLSAFGTEAEPVRARLRQEVQPGITIRALAKLAAFLNGAGIPAGIASQRLMLGKAAT
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
RLLELLCSSARLTWPAPAAAPDPHALFTLFCHREPAGCEQLILPLAEGILPEDRCRHLAA
HHHHHHHHCCCCCCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHH
YLTRQHIPRGGRLLLTGDDIMILLGLPPGRQVGEAIELLRAAQSTGEVRTRAEAQRYLAK
HHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQLTTPEPLR
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]