Click here to switch to the map view.

The map label for this gene is czcB [H]

Identifier: 39997234

GI number: 39997234

Start: 2353324

End: 2354580

Strand: Reverse

Name: czcB [H]

Synonym: GSU2136

Alternate gene names: 39997234

Gene position: 2354580-2353324 (Counterclockwise)

Preceding gene: 39997235

Following gene: 39997233

Centisome position: 61.73

GC content: 60.38

Gene sequence:

>1257_bases
GTGAACAAGAAAGCAATCATCATCGGACTTATCCTGGCCCTGGCCCTGGGGGGCGGCGTCGCTTACCGGCTGACCCATTC
CACCACCGGGGGCGGAGAAAAAGCCGAACAAAAAGAGGCCGGCCATAAGGACGAAAAAGGCGGTCACGACGAAGGGAAGG
AAGAGAAGGAAAAGAAGGAAGAGCATGACGAGCACGGCGAGGAAAAACTCGTCAAGATGTCCGCCGAGGTACAGAAACAG
AGCGGCGTGGTCGTGGCGCCGGCAAAAAAGCAGCGCCTGGCTGGCGTCATCAGCGCCACCGGCAAGGTGGAGGCCAATGC
CGACCGGATCGCCCATGTCTCCCCGCGCATCCCCGGCAAGATAGTGGCGGTCAGGTCTTCCCTGGGTGACAGCGTTGCTG
CCGGACAGGTGCTCGTCACGCTGGACAGCGTCGAGCTGGGTGAAGCGCTCAATCGCTACCACCAGTCACGGACCAAGCTG
GCCCTGGCCCAATCCAACATGGATCGGATCAAGGTGTTGGTAGACAAGAAGATTGCGGCCCGCAAGGAGATCCTCCAGGC
TGAGACCGATTACAAGACCGCCCAGACCGAGCTGCATACCGACGAAGAGCGTCTCTCTCTGTACGGTATCAACCTCTCCG
ATCTCAAGTCAGATAACCACAAGAAGCCGCTCCTGCCGGTGCGGTCGCCGATCGGCGGGGTCATCACCGAGAAACACGCC
ATTGTCGGCGAGCTGTCCGACCCCTCCAAGAGCCTTTACACCGTGGCCGACCTGTCGTCGGTCTGGGTGCTGGTGGATAT
TCACGAAAAGGACCTGGCCAAGGTCCGCCGGGGACAGGCGGCAACCGTCATCGTCGGCGCCTTTCCCGACAAGAAGTTCC
GCGGTCGGATAACCTATCTTGCCGACCTGGTTGACGAGGCTACCCGCACGGTCAAGGCACGGGTCGAAGTACCCAATCCG
GGCCGCAAGCTGAAACCGGAGATGTTCGCCACCGTCGAACTGGCGATTGCCGCAGACGCGCCGCCGGTGCTGGCGGTGCC
TGAAGAGGCCGTACTGGAACTGGACGGCAAGAAGGTGATCTTTGTCGCTGAAAAGGAGACCGAGTTCGAACCGAGGGTCG
TAGAGCTGGGAAGGACTGTGGGCGGCATGGTGGAAGTGGTTTCCGGCCTCAAGGAAGGTGAACGCTATGCCACCAAGGGC
GCTTTCACCCTGAAGTCGGAACTGAAGAAGGAAGAACTAGAGGACGACGAACACTGA

Upstream 100 bases:

>100_bases
TCGAGGTCAGCGACGGCTATCTGACGGCGCTCCATGACCGGCAGCTGGCACTCGTAAAACTCGAATCAGCAGTGGCGACA
GACATCAACGGAGGTGTGCA

Downstream 100 bases:

>100_bases
GGAGATAACCACCCATGCTAGAAAAACTCATCGCATATACCTTGAAGCAGAAAGGGATGGTCATCTTCGCTGCGCTGGTG
ATCGTTGTTTTCGGCTTCTA

Product: CzcB family heavy metal efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNKKAIIIGLILALALGGGVAYRLTHSTTGGGEKAEQKEAGHKDEKGGHDEGKEEKEKKEEHDEHGEEKLVKMSAEVQKQ
SGVVVAPAKKQRLAGVISATGKVEANADRIAHVSPRIPGKIVAVRSSLGDSVAAGQVLVTLDSVELGEALNRYHQSRTKL
ALAQSNMDRIKVLVDKKIAARKEILQAETDYKTAQTELHTDEERLSLYGINLSDLKSDNHKKPLLPVRSPIGGVITEKHA
IVGELSDPSKSLYTVADLSSVWVLVDIHEKDLAKVRRGQAATVIVGAFPDKKFRGRITYLADLVDEATRTVKARVEVPNP
GRKLKPEMFATVELAIAADAPPVLAVPEEAVLELDGKKVIFVAEKETEFEPRVVELGRTVGGMVEVVSGLKEGERYATKG
AFTLKSELKKEELEDDEH

Sequences:

>Translated_418_residues
MNKKAIIIGLILALALGGGVAYRLTHSTTGGGEKAEQKEAGHKDEKGGHDEGKEEKEKKEEHDEHGEEKLVKMSAEVQKQ
SGVVVAPAKKQRLAGVISATGKVEANADRIAHVSPRIPGKIVAVRSSLGDSVAAGQVLVTLDSVELGEALNRYHQSRTKL
ALAQSNMDRIKVLVDKKIAARKEILQAETDYKTAQTELHTDEERLSLYGINLSDLKSDNHKKPLLPVRSPIGGVITEKHA
IVGELSDPSKSLYTVADLSSVWVLVDIHEKDLAKVRRGQAATVIVGAFPDKKFRGRITYLADLVDEATRTVKARVEVPNP
GRKLKPEMFATVELAIAADAPPVLAVPEEAVLELDGKKVIFVAEKETEFEPRVVELGRTVGGMVEVVSGLKEGERYATKG
AFTLKSELKKEELEDDEH
>Mature_418_residues
MNKKAIIIGLILALALGGGVAYRLTHSTTGGGEKAEQKEAGHKDEKGGHDEGKEEKEKKEEHDEHGEEKLVKMSAEVQKQ
SGVVVAPAKKQRLAGVISATGKVEANADRIAHVSPRIPGKIVAVRSSLGDSVAAGQVLVTLDSVELGEALNRYHQSRTKL
ALAQSNMDRIKVLVDKKIAARKEILQAETDYKTAQTELHTDEERLSLYGINLSDLKSDNHKKPLLPVRSPIGGVITEKHA
IVGELSDPSKSLYTVADLSSVWVLVDIHEKDLAKVRRGQAATVIVGAFPDKKFRGRITYLADLVDEATRTVKARVEVPNP
GRKLKPEMFATVELAIAADAPPVLAVPEEAVLELDGKKVIFVAEKETEFEPRVVELGRTVGGMVEVVSGLKEGERYATKG
AFTLKSELKKEELEDDEH

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=431, Percent_Identity=25.9860788863109, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789929, Length=328, Percent_Identity=25.3048780487805, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081796, Length=341, Percent_Identity=24.0469208211144, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45404; Mature: 45404

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKAIIIGLILALALGGGVAYRLTHSTTGGGEKAEQKEAGHKDEKGGHDEGKEEKEKKE
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHH
EHDEHGEEKLVKMSAEVQKQSGVVVAPAKKQRLAGVISATGKVEANADRIAHVSPRIPGK
HHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCCCCEECCCCCEEECCCCCCCE
IVAVRSSLGDSVAAGQVLVTLDSVELGEALNRYHQSRTKLALAQSNMDRIKVLVDKKIAA
EEEEEHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHH
RKEILQAETDYKTAQTELHTDEERLSLYGINLSDLKSDNHKKPLLPVRSPIGGVITEKHA
HHHHHHHHCCCHHHHHHCCCCCHHEEEECCCHHHCCCCCCCCCCCCCCCCCCCCEECCCC
IVGELSDPSKSLYTVADLSSVWVLVDIHEKDLAKVRRGQAATVIVGAFPDKKFRGRITYL
EEECCCCCCCCEEEEEECCCEEEEEEECHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
ADLVDEATRTVKARVEVPNPGRKLKPEMFATVELAIAADAPPVLAVPEEAVLELDGKKVI
HHHHHHHHHEEEEEEECCCCCCCCCCHHEEEEEEEEECCCCCEEECCCHHHEEECCCEEE
FVAEKETEFEPRVVELGRTVGGMVEVVSGLKEGERYATKGAFTLKSELKKEELEDDEH
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKKAIIIGLILALALGGGVAYRLTHSTTGGGEKAEQKEAGHKDEKGGHDEGKEEKEKKE
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHH
EHDEHGEEKLVKMSAEVQKQSGVVVAPAKKQRLAGVISATGKVEANADRIAHVSPRIPGK
HHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHCCCCEECCCCCEEECCCCCCCE
IVAVRSSLGDSVAAGQVLVTLDSVELGEALNRYHQSRTKLALAQSNMDRIKVLVDKKIAA
EEEEEHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHH
RKEILQAETDYKTAQTELHTDEERLSLYGINLSDLKSDNHKKPLLPVRSPIGGVITEKHA
HHHHHHHHCCCHHHHHHCCCCCHHEEEECCCHHHCCCCCCCCCCCCCCCCCCCCEECCCC
IVGELSDPSKSLYTVADLSSVWVLVDIHEKDLAKVRRGQAATVIVGAFPDKKFRGRITYL
EEECCCCCCCCEEEEEECCCEEEEEEECHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
ADLVDEATRTVKARVEVPNPGRKLKPEMFATVELAIAADAPPVLAVPEEAVLELDGKKVI
HHHHHHHHHEEEEEEECCCCCCCCCCHHEEEEEEEEECCCCCEEECCCHHHEEECCCEEE
FVAEKETEFEPRVVELGRTVGGMVEVVSGLKEGERYATKGAFTLKSELKKEELEDDEH
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]