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The map label for this gene is hldE

Identifier: 39997183

GI number: 39997183

Start: 2290675

End: 2292147

Strand: Reverse

Name: hldE

Synonym: GSU2085

Alternate gene names: 39997183

Gene position: 2292147-2290675 (Counterclockwise)

Preceding gene: 39997184

Following gene: 39997182

Centisome position: 60.1

GC content: 65.51

Gene sequence:

>1473_bases
ATGGAGCGCAAGAACGTCGAATCTCTTTTTGCCCATGCCGGGGCGATCAACGCCCTGGTGATCGGCGACCTGATGCTCGA
CCAGTACCTGTGGGGCAAGGCCGAGCGGATTTCCCCGGAGGCGCCGGTTCAGGTGGTGGACGTGACCCGGGAGGAGATCC
GGATCGGCGGCGCCGGCAACGTGGCCAACAACCTGGTGGCCCTCGGCTGCCGGGTGAGCGTGGCGAGCGTGGTGGGGGGG
GACGAGAACGGCACCATTCTCCTCCATGCCTTTTCCGGCAAGGGGATCGACGTGACCGGCATCGTGGAGGACCCGTCGCG
CACTACCAGCCGAAAGACCCGCGTCCTCGCCTCAAACCAGCAGATCGTCCGGATTGACCGGGAATCCCGGGACGAGATCG
GCCCGGACAACGAGCGGCGCATCGTCGACTACCTGAACGCCCACGGCGACCGCTTCAACGTGATCCTCGTGTCGGACTAT
CTGAAGGGGGTCCTGACCCCGACGCTCCTGGCCGAGGTGATCGCTTTTGCCCGCAAGCGCGAAATCCCCGTCGTGGTTGA
TCCAAAGGGGAGCGACTACGCGAAATACCGGGGTGCCACGGTTTTGACCCCCAACCGGAAGGAGGCGGAAGCGGCCTCCG
GCATCGCCATCCGCGACGATGAGAGCCTCTGCCGGGCCGGCGAGCGGCTGCTGGAGACGGCCGATCTGACCGCTCTGGTC
ATCACCCGGAGCGAGGAGGGGATGTCGCTCTTCCTGCGGGGCGGCCAGGTGGTGCATATTCCCACCTATGCCCGCGAGGT
ATTCGACGTGACCGGCGCCGGCGATACCGTGCTGGCCGTGCTCGGCATGGCCCTGGCCGGCGGGGTCGGTTTCGCCGACG
GGGCCCGGCTGGCCAATGTGGCGGCCGGCGTGGCGGTGGGGAAGGTGGGCACGTCCACGGTATCTCCTGCCGAGATTGTC
GGATCGCTGGGATTCCAGCACGGCGAGGGGGACGCCAAGGTCAAGAACCTGGATGTCCTCGCCGGCATCATCGCGGCGGA
AAAGGCGCAGGGCAAGCGGGTGGTGTTCACCAACGGCTGTTTCGACCTTCTGCACGTGGGGCACGTCAAATATCTCCAGA
AGGCCCGCAGCTTCGGCGACCTGCTGGTCCTGGGTCTCAACAGCGATGCCTCCATCCGGCGGCTCAAGGGTGAAAAGCGC
CCGCTCATCGGCCAGGAGGAACGGGCCCACATCCTTGCTGCCCTGGATTGCATCGATTACGTGGTCGTTTTCGATGAAGA
CACTCCGCTCAACCTGATCGAGACCCTCAGACCGGCGGTGCTGGTCAAGGGAGGCGACTACACCCTCGACGGGGTAGTGG
GGCGCGAGGTGGTCGAATCCTACGGAGGGCGGGTGGAACTGGTGGCCTTCGTGGACGGTCGCTCCACGACGAACATCATC
GAGAAGATTCTGAAGGCCTACGGCGAGGAGTGA

Upstream 100 bases:

>100_bases
TCGGGCAAGGGACGGGGATACACCATCACCGGCCACCACGAGATCATGATCCCGCTTCTGGCGGCGGGCATCCTGGACCG
GATACGGGGGTAGGGGAGCC

Downstream 100 bases:

>100_bases
CCGGTGACGGGTGGCAGACGCGCTGTTTTTCTCGACCGTGACGGCACCATCAATGTTGAAAAGGGCTATGTCCACCAAGC
CCATGAATTCGAGTTCGTCC

Product: ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MERKNVESLFAHAGAINALVIGDLMLDQYLWGKAERISPEAPVQVVDVTREEIRIGGAGNVANNLVALGCRVSVASVVGG
DENGTILLHAFSGKGIDVTGIVEDPSRTTSRKTRVLASNQQIVRIDRESRDEIGPDNERRIVDYLNAHGDRFNVILVSDY
LKGVLTPTLLAEVIAFARKREIPVVVDPKGSDYAKYRGATVLTPNRKEAEAASGIAIRDDESLCRAGERLLETADLTALV
ITRSEEGMSLFLRGGQVVHIPTYAREVFDVTGAGDTVLAVLGMALAGGVGFADGARLANVAAGVAVGKVGTSTVSPAEIV
GSLGFQHGEGDAKVKNLDVLAGIIAAEKAQGKRVVFTNGCFDLLHVGHVKYLQKARSFGDLLVLGLNSDASIRRLKGEKR
PLIGQEERAHILAALDCIDYVVVFDEDTPLNLIETLRPAVLVKGGDYTLDGVVGREVVESYGGRVELVAFVDGRSTTNII
EKILKAYGEE

Sequences:

>Translated_490_residues
MERKNVESLFAHAGAINALVIGDLMLDQYLWGKAERISPEAPVQVVDVTREEIRIGGAGNVANNLVALGCRVSVASVVGG
DENGTILLHAFSGKGIDVTGIVEDPSRTTSRKTRVLASNQQIVRIDRESRDEIGPDNERRIVDYLNAHGDRFNVILVSDY
LKGVLTPTLLAEVIAFARKREIPVVVDPKGSDYAKYRGATVLTPNRKEAEAASGIAIRDDESLCRAGERLLETADLTALV
ITRSEEGMSLFLRGGQVVHIPTYAREVFDVTGAGDTVLAVLGMALAGGVGFADGARLANVAAGVAVGKVGTSTVSPAEIV
GSLGFQHGEGDAKVKNLDVLAGIIAAEKAQGKRVVFTNGCFDLLHVGHVKYLQKARSFGDLLVLGLNSDASIRRLKGEKR
PLIGQEERAHILAALDCIDYVVVFDEDTPLNLIETLRPAVLVKGGDYTLDGVVGREVVESYGGRVELVAFVDGRSTTNII
EKILKAYGEE
>Mature_490_residues
MERKNVESLFAHAGAINALVIGDLMLDQYLWGKAERISPEAPVQVVDVTREEIRIGGAGNVANNLVALGCRVSVASVVGG
DENGTILLHAFSGKGIDVTGIVEDPSRTTSRKTRVLASNQQIVRIDRESRDEIGPDNERRIVDYLNAHGDRFNVILVSDY
LKGVLTPTLLAEVIAFARKREIPVVVDPKGSDYAKYRGATVLTPNRKEAEAASGIAIRDDESLCRAGERLLETADLTALV
ITRSEEGMSLFLRGGQVVHIPTYAREVFDVTGAGDTVLAVLGMALAGGVGFADGARLANVAAGVAVGKVGTSTVSPAEIV
GSLGFQHGEGDAKVKNLDVLAGIIAAEKAQGKRVVFTNGCFDLLHVGHVKYLQKARSFGDLLVLGLNSDASIRRLKGEKR
PLIGQEERAHILAALDCIDYVVVFDEDTPLNLIETLRPAVLVKGGDYTLDGVVGREVVESYGGRVELVAFVDGRSTTNII
EKILKAYGEE

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Homo sapiens, GI296841136, Length=133, Percent_Identity=33.8345864661654, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI4505651, Length=133, Percent_Identity=33.8345864661654, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1789432, Length=479, Percent_Identity=44.258872651357, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788491, Length=261, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_GEOSL (Q74BF6)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953134.1
- ProteinModelPortal:   Q74BF6
- GeneID:   2687883
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2085
- NMPDR:   fig|243231.1.peg.2072
- TIGR:   GSU2085
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   CLSK828725
- BioCyc:   GSUL243231:GSU_2085-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 52359; Mature: 52359

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKNVESLFAHAGAINALVIGDLMLDQYLWGKAERISPEAPVQVVDVTREEIRIGGAGN
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHEEECCCCC
VANNLVALGCRVSVASVVGGDENGTILLHAFSGKGIDVTGIVEDPSRTTSRKTRVLASNQ
HHCCCEEEECCEEEEHHCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHEEEECCC
QIVRIDRESRDEIGPDNERRIVDYLNAHGDRFNVILVSDYLKGVLTPTLLAEVIAFARKR
EEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHCCHHHHHHHHHHHHHC
EIPVVVDPKGSDYAKYRGATVLTPNRKEAEAASGIAIRDDESLCRAGERLLETADLTALV
CCCEEECCCCCCHHHHCCCEEECCCCCHHHHHCCCEEECCHHHHHHHHHHHHHCCCEEEE
ITRSEEGMSLFLRGGQVVHIPTYAREVFDVTGAGDTVLAVLGMALAGGVGFADGARLANV
EEECCCCCEEEEECCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
AAGVAVGKVGTSTVSPAEIVGSLGFQHGEGDAKVKNLDVLAGIIAAEKAQGKRVVFTNGC
HHHHEECCCCCCCCCHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCC
FDLLHVGHVKYLQKARSFGDLLVLGLNSDASIRRLKGEKRPLIGQEERAHILAALDCIDY
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHEEE
VVVFDEDTPLNLIETLRPAVLVKGGDYTLDGVVGREVVESYGGRVELVAFVDGRSTTNII
EEEECCCCCHHHHHHCCCEEEEECCCEEECCHHHHHHHHHCCCEEEEEEEECCCCHHHHH
EKILKAYGEE
HHHHHHHCCC
>Mature Secondary Structure
MERKNVESLFAHAGAINALVIGDLMLDQYLWGKAERISPEAPVQVVDVTREEIRIGGAGN
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHEEECCCCC
VANNLVALGCRVSVASVVGGDENGTILLHAFSGKGIDVTGIVEDPSRTTSRKTRVLASNQ
HHCCCEEEECCEEEEHHCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHEEEECCC
QIVRIDRESRDEIGPDNERRIVDYLNAHGDRFNVILVSDYLKGVLTPTLLAEVIAFARKR
EEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHCCHHHHHHHHHHHHHC
EIPVVVDPKGSDYAKYRGATVLTPNRKEAEAASGIAIRDDESLCRAGERLLETADLTALV
CCCEEECCCCCCHHHHCCCEEECCCCCHHHHHCCCEEECCHHHHHHHHHHHHHCCCEEEE
ITRSEEGMSLFLRGGQVVHIPTYAREVFDVTGAGDTVLAVLGMALAGGVGFADGARLANV
EEECCCCCEEEEECCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
AAGVAVGKVGTSTVSPAEIVGSLGFQHGEGDAKVKNLDVLAGIIAAEKAQGKRVVFTNGC
HHHHEECCCCCCCCCHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCC
FDLLHVGHVKYLQKARSFGDLLVLGLNSDASIRRLKGEKRPLIGQEERAHILAALDCIDY
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHEEE
VVVFDEDTPLNLIETLRPAVLVKGGDYTLDGVVGREVVESYGGRVELVAFVDGRSTTNII
EEEECCCCCHHHHHHCCCEEEEECCCEEECCHHHHHHHHHCCCEEEEEEEECCCCHHHHH
EKILKAYGEE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA