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The map label for this gene is rfbA [H]

Identifier: 39997181

GI number: 39997181

Start: 2289112

End: 2290014

Strand: Reverse

Name: rfbA [H]

Synonym: GSU2083

Alternate gene names: 39997181

Gene position: 2290014-2289112 (Counterclockwise)

Preceding gene: 39997182

Following gene: 39997180

Centisome position: 60.04

GC content: 61.13

Gene sequence:

>903_bases
ATGGCCATTTCCAAGGGAATCATACTTGCCGGCGGAGCCGGCAGCCGTCTGTATCCTCTCACGCTCGTCGCCAGCAAGCA
GCTCCAGCCGGTCTACGACAAGCCGATGATCTACTATCCCCTTGCCACGCTCATGATGGCGGGGATCAAGGACATCCTGA
TCATTTCTACCCCCCACGATACCCCCCGCTTCCAGGCGCTCCTGGGCGACGGCTCCCGGTGGGGGATAACCCTTACCTAC
AAAGTGCAGCCCGAACCCAAGGGAATCGCCCAGGCGTTCCTGGTGGGCGAGGAGTTCATCGCCGGTGACCCGGTCTGCCT
GATCCTGGGAGACAACATTTTTTACGGCAAGATGGGGCTCGACCGTCTGGTGCAGGATTTCACGACGGGAGCCTGGATCT
TCGGGTACTACGTGAACGACCCCGAACGCTACGGCGTCGTCCAGTTCGACGGCAACGGCAAGGCCATCGGCATCGAGGAG
AAGCCTGCCCAGCCCAAAAGCAACTATGCCGTCCCCGGCCTCTATCTCTACGACGGCAGGGTTGTGGACGTCGCCCGCAA
CCTGGCGCCGTCTCCCCGGGGTGAGCTGGAGATTACCGACGTGAACCTTGATTACCTGCGGCGGGGCGAACTCATGGTGG
AAAGACTCGGGCGCGGTATTGCCTGGCTCGATACCGGAACCCACCAGAGCCTGCTGGAGGCATCACATTTCATCGGTACC
CTCGAGGCACGCCAGGGGTTGAAGATCGCCTGCCTGGAGGAGATCGCCCTGCGTATGGGATTCATCGACTGCCGCCGGAT
GGCCGAGGTTATCGCCGAGACCCCCAAGTCGTCCTACCGGGACTACCTGGCCCGTATCTACAATGAAACCGAACTGTGCG
GAGGTGTTGTTCATGAGCGTTAG

Upstream 100 bases:

>100_bases
GGAGGGAGATAGCGTTTGACGCTGTTTTGCCCGCTGTGCTATAACTACTGATTTAAAGAGATGTTTTCGGTGTCGAGGGC
ACTGAAGTTCAGGAGAGTCC

Downstream 100 bases:

>100_bases
TCCCGAGTTCACCCGCGGCAAGATCCACGACGTGGTGGTGAAGCCCCTTGGCAAGTTCCTGGACGAGCGGGGCTGGCTCG
CCGAACTGTTCCGCAGCGAC

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAISKGIILAGGAGSRLYPLTLVASKQLQPVYDKPMIYYPLATLMMAGIKDILIISTPHDTPRFQALLGDGSRWGITLTY
KVQPEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMGLDRLVQDFTTGAWIFGYYVNDPERYGVVQFDGNGKAIGIEE
KPAQPKSNYAVPGLYLYDGRVVDVARNLAPSPRGELEITDVNLDYLRRGELMVERLGRGIAWLDTGTHQSLLEASHFIGT
LEARQGLKIACLEEIALRMGFIDCRRMAEVIAETPKSSYRDYLARIYNETELCGGVVHER

Sequences:

>Translated_300_residues
MAISKGIILAGGAGSRLYPLTLVASKQLQPVYDKPMIYYPLATLMMAGIKDILIISTPHDTPRFQALLGDGSRWGITLTY
KVQPEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMGLDRLVQDFTTGAWIFGYYVNDPERYGVVQFDGNGKAIGIEE
KPAQPKSNYAVPGLYLYDGRVVDVARNLAPSPRGELEITDVNLDYLRRGELMVERLGRGIAWLDTGTHQSLLEASHFIGT
LEARQGLKIACLEEIALRMGFIDCRRMAEVIAETPKSSYRDYLARIYNETELCGGVVHER
>Mature_299_residues
AISKGIILAGGAGSRLYPLTLVASKQLQPVYDKPMIYYPLATLMMAGIKDILIISTPHDTPRFQALLGDGSRWGITLTYK
VQPEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMGLDRLVQDFTTGAWIFGYYVNDPERYGVVQFDGNGKAIGIEEK
PAQPKSNYAVPGLYLYDGRVVDVARNLAPSPRGELEITDVNLDYLRRGELMVERLGRGIAWLDTGTHQSLLEASHFIGTL
EARQGLKIACLEEIALRMGFIDCRRMAEVIAETPKSSYRDYLARIYNETELCGGVVHER

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=294, Percent_Identity=62.9251700680272, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=59.6491228070175, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI133931050, Length=182, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355443, Length=252, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24644084, Length=252, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 33212; Mature: 33081

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISKGIILAGGAGSRLYPLTLVASKQLQPVYDKPMIYYPLATLMMAGIKDILIISTPHD
CCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCC
TPRFQALLGDGSRWGITLTYKVQPEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMGL
CCHHHHEECCCCEEEEEEEEEECCCCCHHHHHHHHCCHHHCCCCEEEEEECCEEEECCCH
DRLVQDFTTGAWIFGYYVNDPERYGVVQFDGNGKAIGIEEKPAQPKSNYAVPGLYLYDGR
HHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCEEEEECCE
VVDVARNLAPSPRGELEITDVNLDYLRRGELMVERLGRGIAWLDTGTHQSLLEASHFIGT
EEEHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHEE
LEARQGLKIACLEEIALRMGFIDCRRMAEVIAETPKSSYRDYLARIYNETELCGGVVHER
HHHCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHCHHHCCC
>Mature Secondary Structure 
AISKGIILAGGAGSRLYPLTLVASKQLQPVYDKPMIYYPLATLMMAGIKDILIISTPHD
CCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCC
TPRFQALLGDGSRWGITLTYKVQPEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMGL
CCHHHHEECCCCEEEEEEEEEECCCCCHHHHHHHHCCHHHCCCCEEEEEECCEEEECCCH
DRLVQDFTTGAWIFGYYVNDPERYGVVQFDGNGKAIGIEEKPAQPKSNYAVPGLYLYDGR
HHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCEEEEECCE
VVDVARNLAPSPRGELEITDVNLDYLRRGELMVERLGRGIAWLDTGTHQSLLEASHFIGT
EEEHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHEE
LEARQGLKIACLEEIALRMGFIDCRRMAEVIAETPKSSYRDYLARIYNETELCGGVVHER
HHHCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]