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The map label for this gene is rnhA

Identifier: 39997169

GI number: 39997169

Start: 2272223

End: 2272672

Strand: Reverse

Name: rnhA

Synonym: GSU2071

Alternate gene names: 39997169

Gene position: 2272672-2272223 (Counterclockwise)

Preceding gene: 39997170

Following gene: 39997168

Centisome position: 59.59

GC content: 62.67

Gene sequence:

>450_bases
ATGTCGGCAGAAGTTGAGGTGTTCTGCGACGGCGCCTGTAGTGGTAACCCCGGAGTGGGGGGATACGGCGCGATCCTGCG
TTACGGCAGCGCGGAAAAGGAGCTTTCGGGCGCCGATGGCGATACAACCAACAACCGGATGGAGCTTACCGCTGCCATCA
GGGCGCTGGAAGCCCTCAGCCGTCCCTGTGCCGTGACGATCACCACCGATTCCCAATATCTGGTGAAGGGGATGACTGAA
TGGCTTTCCGGTTGGGTGCGTCGGGGATGGGTGAACAGCAAGAAGGAACCGGTCCTGAACCGTGATCTCTGGGAGCGGCT
CAGGGAGCTGACCGGGAAGCATCAGGTCAGGTGGGTCTGGGTACGAGGCCACAACGGACACCCGGAGAACGAGCGGTGCG
ATGCCCTGGCGCGCCGTGCCATCGATGCGTACCGGAACGAACGCCGGTAG

Upstream 100 bases:

>100_bases
GACGATCTTTTTGCCCGAGATGACTGCAAGGAAACCCACCAACTGATCGTGGATCGGGTAATGGCTGCCATTGCCGACTT
GCTCAAGGGGGAGGATGCCT

Downstream 100 bases:

>100_bases
CGTGTGGCAGGGAGCCATGACCTATTTCGTAGTGGAAGTGACGGACCAGGAGATCCGGCGGGAGAAGGACAAAGCCCGAG
AACTGCGCCGGAGCCGCTGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSAEVEVFCDGACSGNPGVGGYGAILRYGSAEKELSGADGDTTNNRMELTAAIRALEALSRPCAVTITTDSQYLVKGMTE
WLSGWVRRGWVNSKKEPVLNRDLWERLRELTGKHQVRWVWVRGHNGHPENERCDALARRAIDAYRNERR

Sequences:

>Translated_149_residues
MSAEVEVFCDGACSGNPGVGGYGAILRYGSAEKELSGADGDTTNNRMELTAAIRALEALSRPCAVTITTDSQYLVKGMTE
WLSGWVRRGWVNSKKEPVLNRDLWERLRELTGKHQVRWVWVRGHNGHPENERCDALARRAIDAYRNERR
>Mature_148_residues
SAEVEVFCDGACSGNPGVGGYGAILRYGSAEKELSGADGDTTNNRMELTAAIRALEALSRPCAVTITTDSQYLVKGMTEW
LSGWVRRGWVNSKKEPVLNRDLWERLRELTGKHQVRWVWVRGHNGHPENERCDALARRAIDAYRNERR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=149, Percent_Identity=33.5570469798658, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1786408, Length=140, Percent_Identity=59.2857142857143, Blast_Score=179, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI115532542, Length=143, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552509, Length=153, Percent_Identity=32.6797385620915, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137476, Length=153, Percent_Identity=32.6797385620915, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_GEOSL (Q74BH0)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953120.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q74BH0
- SMR:   Q74BH0
- GeneID:   2687939
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2071
- NMPDR:   fig|243231.1.peg.2058
- TIGR:   GSU2071
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   GSUL243231:GSU_2071-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16748; Mature: 16617

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEVEVFCDGACSGNPGVGGYGAILRYGSAEKELSGADGDTTNNRMELTAAIRALEALS
CCCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
RPCAVTITTDSQYLVKGMTEWLSGWVRRGWVNSKKEPVLNRDLWERLRELTGKHQVRWVW
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEE
VRGHNGHPENERCDALARRAIDAYRNERR
EECCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAEVEVFCDGACSGNPGVGGYGAILRYGSAEKELSGADGDTTNNRMELTAAIRALEALS
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
RPCAVTITTDSQYLVKGMTEWLSGWVRRGWVNSKKEPVLNRDLWERLRELTGKHQVRWVW
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEE
VRGHNGHPENERCDALARRAIDAYRNERR
EECCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA