Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 39997158

GI number: 39997158

Start: 2259760

End: 2261100

Strand: Reverse

Name: pmbA [H]

Synonym: GSU2060

Alternate gene names: 39997158

Gene position: 2261100-2259760 (Counterclockwise)

Preceding gene: 39997159

Following gene: 39997156

Centisome position: 59.28

GC content: 64.21

Gene sequence:

>1341_bases
ATGGATACCGCCGGCATGATCAGCGCCGTTCAAGAAATACTCGCCAACCGTTCCCTGGACGGATGGGAGATAACCGCCGC
ATCGTCGCACATTCTTTCTATCGAGGTGAAGGAACAAAAGGTCGATACCTTCAAGTGTTCCGCCCCGCTGGGGGTGAGCG
TGCGCGTGCTGAAGAACGGCGGCATGGGGTTCTCCTTTTCCACAAGCATGGCACCGGAGGACTTGGCGCGCATGGTGGAC
AACGCCCTTGTGGGTGCCGAAAGCCAGACCCCGGACGCGGACCACGGTTTCCCCGACCCGCCGGCGGTATATCCCGATGT
TCCGGGGCTGTTCGACGACGGGCTTGCTGCCGTGGCCGAAGAGGCAAAGATTGCCGTCGCACTCGACCTTGAGCGGCTTA
CCCTGGCGGCCGATCCGCGGGTGCGGAAGGTGCGGAAAGCCACCTATGGTGATTCCATGGTGGAGACTATGGTGGTCAAT
TCCCGGGGCGTCAGCTGCCGCTACCGGGGAACATCGGTGTCGGCCAGCGTGACGGCCGTTGCCGAGGAAAACGGTCAGTC
CCAGATGGGGTGGGATTTCGATTTCTCATCCCGCTTCGACGGCATTTCGGTGGAGCGGATCGTTGCCGGCGCAGTTGCCA
AGGCGACCGGCCTCCTGGGCGCCGCCACGGTGCCGACCATGCGCTGCCCCGCGGTGCTCGACACCTACGTGGCCACCGAG
ATCCTCGAAGTGCTGGCACCGGCATTCCTGGCCGAGAGCGTTCAAAAGGGCAAGTCGCTCCTGGCGGGCAAGCGGGGCGA
GATGATCGTTGCGCCGCTGCTCACCATCCGGGACGACGGTACCCTGCCCGGCGGCATGGGAACCGCTCCCTTCGATGCGG
AGGGCGTGCCCAAGCGGAATACGGTTCTGGTGCAAAACGGGCGGCTCGACAGCTATCTCTACGATACGCTCCGGGCGCGC
AAGGAGGGGGTCGCCCCTACCGGAAACGCCATTCGCAGCGGCATCAAATCGCCGCCGCACATGGGGGTATCCAATCTCTT
CATCGAGAACGGCACCGAAGCTCCCGAAGCGCTCCTGGCGGGAATCACGCGGGGAGTGCTGCTGACCGAGGTAATGGGGA
TGCATACCGCCAATACCATTTCGGGTGACTTCTCCGTGGGCGCAGCGGGCTTCCTGGTGGAGGACGGACGCGTGACCCGG
CCGGTGAAGGGGATCGCCATTGCGGGCAACATCTTGGAGCTGTTCAGGGGGGTCGAGGCGGTGGGGAATGACCTGCGCTT
CTACGGTACCGTGGGCTCGCCGTCGTTGCGGATTGCCGCCCTGGACGTGAGCGGGGAGTAA

Upstream 100 bases:

>100_bases
GTCGGAGTTGCCCCACAAGGTCTGGACCGACTGCATCAAGTGCGTCAAGTTCCCCGACTGCGATGAAATCGCGATGATTC
TTGATCTCTGAGAGGTTTCC

Downstream 100 bases:

>100_bases
GTCCGGCCGCGCCATTTGCCGTATACCGAGGAAAAAGCACAAACAGCCGCCCGGCATGACCGGCGCGGCTGTTTTTCGTG
GGGGACGTTCTGGTGCGTGG

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDTAGMISAVQEILANRSLDGWEITAASSHILSIEVKEQKVDTFKCSAPLGVSVRVLKNGGMGFSFSTSMAPEDLARMVD
NALVGAESQTPDADHGFPDPPAVYPDVPGLFDDGLAAVAEEAKIAVALDLERLTLAADPRVRKVRKATYGDSMVETMVVN
SRGVSCRYRGTSVSASVTAVAEENGQSQMGWDFDFSSRFDGISVERIVAGAVAKATGLLGAATVPTMRCPAVLDTYVATE
ILEVLAPAFLAESVQKGKSLLAGKRGEMIVAPLLTIRDDGTLPGGMGTAPFDAEGVPKRNTVLVQNGRLDSYLYDTLRAR
KEGVAPTGNAIRSGIKSPPHMGVSNLFIENGTEAPEALLAGITRGVLLTEVMGMHTANTISGDFSVGAAGFLVEDGRVTR
PVKGIAIAGNILELFRGVEAVGNDLRFYGTVGSPSLRIAALDVSGE

Sequences:

>Translated_446_residues
MDTAGMISAVQEILANRSLDGWEITAASSHILSIEVKEQKVDTFKCSAPLGVSVRVLKNGGMGFSFSTSMAPEDLARMVD
NALVGAESQTPDADHGFPDPPAVYPDVPGLFDDGLAAVAEEAKIAVALDLERLTLAADPRVRKVRKATYGDSMVETMVVN
SRGVSCRYRGTSVSASVTAVAEENGQSQMGWDFDFSSRFDGISVERIVAGAVAKATGLLGAATVPTMRCPAVLDTYVATE
ILEVLAPAFLAESVQKGKSLLAGKRGEMIVAPLLTIRDDGTLPGGMGTAPFDAEGVPKRNTVLVQNGRLDSYLYDTLRAR
KEGVAPTGNAIRSGIKSPPHMGVSNLFIENGTEAPEALLAGITRGVLLTEVMGMHTANTISGDFSVGAAGFLVEDGRVTR
PVKGIAIAGNILELFRGVEAVGNDLRFYGTVGSPSLRIAALDVSGE
>Mature_446_residues
MDTAGMISAVQEILANRSLDGWEITAASSHILSIEVKEQKVDTFKCSAPLGVSVRVLKNGGMGFSFSTSMAPEDLARMVD
NALVGAESQTPDADHGFPDPPAVYPDVPGLFDDGLAAVAEEAKIAVALDLERLTLAADPRVRKVRKATYGDSMVETMVVN
SRGVSCRYRGTSVSASVTAVAEENGQSQMGWDFDFSSRFDGISVERIVAGAVAKATGLLGAATVPTMRCPAVLDTYVATE
ILEVLAPAFLAESVQKGKSLLAGKRGEMIVAPLLTIRDDGTLPGGMGTAPFDAEGVPKRNTVLVQNGRLDSYLYDTLRAR
KEGVAPTGNAIRSGIKSPPHMGVSNLFIENGTEAPEALLAGITRGVLLTEVMGMHTANTISGDFSVGAAGFLVEDGRVTR
PVKGIAIAGNILELFRGVEAVGNDLRFYGTVGSPSLRIAALDVSGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=434, Percent_Identity=27.6497695852535, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789640, Length=464, Percent_Identity=25.8620689655172, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46733; Mature: 46733

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTAGMISAVQEILANRSLDGWEITAASSHILSIEVKEQKVDTFKCSAPLGVSVRVLKNG
CCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCEEECCCCCCCEEEEEECC
GMGFSFSTSMAPEDLARMVDNALVGAESQTPDADHGFPDPPAVYPDVPGLFDDGLAAVAE
CCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHH
EAKIAVALDLERLTLAADPRVRKVRKATYGDSMVETMVVNSRGVSCRYRGTSVSASVTAV
CCEEEEEEEHHHEEEECCHHHHHHHHHHCCCHHHHHHEECCCCCEEEECCCCCCCEEEEE
AEENGQSQMGWDFDFSSRFDGISVERIVAGAVAKATGLLGAATVPTMRCPAVLDTYVATE
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ILEVLAPAFLAESVQKGKSLLAGKRGEMIVAPLLTIRDDGTLPGGMGTAPFDAEGVPKRN
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
TVLVQNGRLDSYLYDTLRARKEGVAPTGNAIRSGIKSPPHMGVSNLFIENGTEAPEALLA
EEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHH
GITRGVLLTEVMGMHTANTISGDFSVGAAGFLVEDGRVTRPVKGIAIAGNILELFRGVEA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHH
VGNDLRFYGTVGSPSLRIAALDVSGE
HCCCEEEEEECCCCCEEEEEEECCCC
>Mature Secondary Structure
MDTAGMISAVQEILANRSLDGWEITAASSHILSIEVKEQKVDTFKCSAPLGVSVRVLKNG
CCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEEECCCCCEEECCCCCCCEEEEEECC
GMGFSFSTSMAPEDLARMVDNALVGAESQTPDADHGFPDPPAVYPDVPGLFDDGLAAVAE
CCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHH
EAKIAVALDLERLTLAADPRVRKVRKATYGDSMVETMVVNSRGVSCRYRGTSVSASVTAV
CCEEEEEEEHHHEEEECCHHHHHHHHHHCCCHHHHHHEECCCCCEEEECCCCCCCEEEEE
AEENGQSQMGWDFDFSSRFDGISVERIVAGAVAKATGLLGAATVPTMRCPAVLDTYVATE
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ILEVLAPAFLAESVQKGKSLLAGKRGEMIVAPLLTIRDDGTLPGGMGTAPFDAEGVPKRN
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
TVLVQNGRLDSYLYDTLRARKEGVAPTGNAIRSGIKSPPHMGVSNLFIENGTEAPEALLA
EEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHH
GITRGVLLTEVMGMHTANTISGDFSVGAAGFLVEDGRVTRPVKGIAIAGNILELFRGVEA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHH
VGNDLRFYGTVGSPSLRIAALDVSGE
HCCCEEEEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]