Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 39997084

GI number: 39997084

Start: 2177234

End: 2178442

Strand: Reverse

Name: wcaJ [H]

Synonym: GSU1986

Alternate gene names: 39997084

Gene position: 2178442-2177234 (Counterclockwise)

Preceding gene: 39997085

Following gene: 39997083

Centisome position: 57.11

GC content: 59.88

Gene sequence:

>1209_bases
ATGCTTTTCTGCGCCTTCTTTGTCGAGGTGTACCACCAGGAGCGGGAGCTGACCCCGCTGGATCTCATCGGGCGCATCAG
CATCGAGCTCGTTCTGGCGTTTTTTGTCCTTTCGGCGCTTTATTTCTTCCTGCCGATCGTTATGGACGGACGGGGCGTGC
TCATGATTGCCACGGTGGTATTCGGATTTTTCCAGTTCCTCTGGCATGCCGGCAGCAGGGGGGGCTTCATATTCCCCTCG
TTCGCCAAGCGAGTGCTTATCCTCGGTACCGGTCCCCTGGCAAACCAGATGGGCAGCCTCGTCACGGCCTCGGGCGCCCG
GTACGTGCTTTCCGGCTACGTGGCCTGCTCCCGTGAGCCGGTCTATGTCCCGGCCCAATCCATCGTCGGGAGCGAGGACG
GCCTCTACGAAACGGTTCGTCGCGCGCGGGCCCACAAGATCGTCGTCTCCCTGGGCGAGCGCCGCGGCGTCTTCCCGCTC
AAAGATGTTCTCAACTGCAAGCTGAGCGGAGTGGAGGTCATGGACGCTCCCTCCTTTTACGAGCGGGTCACCGGCAAGCT
GCTCATCGAGAGCATCAACCCCAGTTGGTTCATCTTCTCGTCCGGTTTCCGCGTAACCGCCCTCAACCGCCTCCTGAAGC
GGGCTCTCGACATTTTCTGCGCACTCTTGGGCGGGGTGCTCTTCCTGCCGTTCCTGCCGATTGTTGCCTTGGCCATCCGG
CTTGACTCGCCCGGCACGATCCTTTTCCGGCAGGAGCGGGTCGGTGAGCGGGAAAAGCCGTTCCATCTTTTCAAATTCAG
GACCATGCGCCAGGACGCCGAGAAGGGGACGGGTGCGGTCTGGGCGCAGAAGGATGATCCGCGCGTTACCAAGCTCGGCC
GTTTTCTCCGCAAGAGCCGCATTGACGAGATACCCCAGTTGTACAACGTTCTGATCGGCGACATGAGCCTGGTGGGACCC
CGGCCGGAGCGTCCCGAGTTCGTGGAGCAGCTCAAGAAGGTCATTCCGTACTATTCCGAACGCCATTTCGTCAAGCCGGG
CGTGACGGGGTGGGCCCAGGTACGATATCCCTACGGGGCATCGGTGGAAGACGCCGTTGAAAAGCTGCGCTACGATCTCT
ACTATATCAAGAATCTGTCAGTTGCCTTTGATATAATGATTATCTTTGAAACCGTGAAGGTCGTACTGTTCCAGCGGGGG
GGGAGGTAA

Upstream 100 bases:

>100_bases
GGCCATACTGGCGGCTCACCTCTTCCGCTTGGGCGAAATACCCGGAGTGGAAAGCTTTACCGGCCAGGGAGGAGTGAGGC
TGGCATTGTTCGTAATCGTC

Downstream 100 bases:

>100_bases
ATCCCCTGGGGACATCCTAAAGGAGATGCGATGCGATTGAAATTTCTGATCTGTGCCCTGTCCCTGCTGTTGCCGATGCT
CGCGTGGAGCGCGGACTATG

Product: glycosyl transferase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLFCAFFVEVYHQERELTPLDLIGRISIELVLAFFVLSALYFFLPIVMDGRGVLMIATVVFGFFQFLWHAGSRGGFIFPS
FAKRVLILGTGPLANQMGSLVTASGARYVLSGYVACSREPVYVPAQSIVGSEDGLYETVRRARAHKIVVSLGERRGVFPL
KDVLNCKLSGVEVMDAPSFYERVTGKLLIESINPSWFIFSSGFRVTALNRLLKRALDIFCALLGGVLFLPFLPIVALAIR
LDSPGTILFRQERVGEREKPFHLFKFRTMRQDAEKGTGAVWAQKDDPRVTKLGRFLRKSRIDEIPQLYNVLIGDMSLVGP
RPERPEFVEQLKKVIPYYSERHFVKPGVTGWAQVRYPYGASVEDAVEKLRYDLYYIKNLSVAFDIMIIFETVKVVLFQRG
GR

Sequences:

>Translated_402_residues
MLFCAFFVEVYHQERELTPLDLIGRISIELVLAFFVLSALYFFLPIVMDGRGVLMIATVVFGFFQFLWHAGSRGGFIFPS
FAKRVLILGTGPLANQMGSLVTASGARYVLSGYVACSREPVYVPAQSIVGSEDGLYETVRRARAHKIVVSLGERRGVFPL
KDVLNCKLSGVEVMDAPSFYERVTGKLLIESINPSWFIFSSGFRVTALNRLLKRALDIFCALLGGVLFLPFLPIVALAIR
LDSPGTILFRQERVGEREKPFHLFKFRTMRQDAEKGTGAVWAQKDDPRVTKLGRFLRKSRIDEIPQLYNVLIGDMSLVGP
RPERPEFVEQLKKVIPYYSERHFVKPGVTGWAQVRYPYGASVEDAVEKLRYDLYYIKNLSVAFDIMIIFETVKVVLFQRG
GR
>Mature_402_residues
MLFCAFFVEVYHQERELTPLDLIGRISIELVLAFFVLSALYFFLPIVMDGRGVLMIATVVFGFFQFLWHAGSRGGFIFPS
FAKRVLILGTGPLANQMGSLVTASGARYVLSGYVACSREPVYVPAQSIVGSEDGLYETVRRARAHKIVVSLGERRGVFPL
KDVLNCKLSGVEVMDAPSFYERVTGKLLIESINPSWFIFSSGFRVTALNRLLKRALDIFCALLGGVLFLPFLPIVALAIR
LDSPGTILFRQERVGEREKPFHLFKFRTMRQDAEKGTGAVWAQKDDPRVTKLGRFLRKSRIDEIPQLYNVLIGDMSLVGP
RPERPEFVEQLKKVIPYYSERHFVKPGVTGWAQVRYPYGASVEDAVEKLRYDLYYIKNLSVAFDIMIIFETVKVVLFQRG
GR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=270, Percent_Identity=37.037037037037, Blast_Score=169, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 45492; Mature: 45492

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFCAFFVEVYHQERELTPLDLIGRISIELVLAFFVLSALYFFLPIVMDGRGVLMIATVV
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FGFFQFLWHAGSRGGFIFPSFAKRVLILGTGPLANQMGSLVTASGARYVLSGYVACSREP
HHHHHHHHHCCCCCCEECHHHHCEEEEEECCHHHHHHCCCEECCCCHHHHHHHHHCCCCC
VYVPAQSIVGSEDGLYETVRRARAHKIVVSLGERRGVFPLKDVLNCKLSGVEVMDAPSFY
EEECHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEECCCHHH
ERVTGKLLIESINPSWFIFSSGFRVTALNRLLKRALDIFCALLGGVLFLPFLPIVALAIR
HHHHHHHHEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LDSPGTILFRQERVGEREKPFHLFKFRTMRQDAEKGTGAVWAQKDDPRVTKLGRFLRKSR
ECCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
IDEIPQLYNVLIGDMSLVGPRPERPEFVEQLKKVIPYYSERHFVKPGVTGWAQVRYPYGA
HHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
SVEDAVEKLRYDLYYIKNLSVAFDIMIIFETVKVVLFQRGGR
CHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLFCAFFVEVYHQERELTPLDLIGRISIELVLAFFVLSALYFFLPIVMDGRGVLMIATVV
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FGFFQFLWHAGSRGGFIFPSFAKRVLILGTGPLANQMGSLVTASGARYVLSGYVACSREP
HHHHHHHHHCCCCCCEECHHHHCEEEEEECCHHHHHHCCCEECCCCHHHHHHHHHCCCCC
VYVPAQSIVGSEDGLYETVRRARAHKIVVSLGERRGVFPLKDVLNCKLSGVEVMDAPSFY
EEECHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCEEEECCCHHH
ERVTGKLLIESINPSWFIFSSGFRVTALNRLLKRALDIFCALLGGVLFLPFLPIVALAIR
HHHHHHHHEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LDSPGTILFRQERVGEREKPFHLFKFRTMRQDAEKGTGAVWAQKDDPRVTKLGRFLRKSR
ECCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
IDEIPQLYNVLIGDMSLVGPRPERPEFVEQLKKVIPYYSERHFVKPGVTGWAQVRYPYGA
HHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
SVEDAVEKLRYDLYYIKNLSVAFDIMIIFETVKVVLFQRGGR
CHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]