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The map label for this gene is epsM [H]

Identifier: 39997071

GI number: 39997071

Start: 2163894

End: 2164523

Strand: Reverse

Name: epsM [H]

Synonym: GSU1973

Alternate gene names: 39997071

Gene position: 2164523-2163894 (Counterclockwise)

Preceding gene: 39997072

Following gene: 39997070

Centisome position: 56.75

GC content: 65.87

Gene sequence:

>630_bases
GTGGCTAGCATTGTCGTGATCGGGGGGGGCGGGCACGCCAAGGTCGTCATCAGCGTGCTGAAAAAGGCCGGCTGCCTGGT
GGCAGGCTATACCGACCGCCAGGATCGGGGGCCGGTCCTCGGCATCCCCTGGCTGGGCGGCGACGGTGTTCTGCCGGGCC
TTCTCGGGACGCATCCGACCTGTGGCGCCATTATCGGCATCGGAAAAGTCGACGCGTCGCAGTTGAGACTCACCCTGCAG
GACGACATCTCCGGACTCGGCTTTGACTTCCCGGTCATCGTGTCGCCGCATGCGGTGGTCAACGAAGACGTGGCGCTCGG
CGCCGGCACGGTCGTCCTCGACGGCGTCGTGGTCAACAGCGGCACGGAGACCGGCCGTGCCTGCATCCTCAACACGAACA
GCACGGTCGAGCACGACTGCCGGCTCGGCGACAACGTCCATATCGCACCGGGGGTAACCCTCAGCGGGGGGGTCGCGGTG
GGGCACAACACCATGGTCGGCACCGGAGCCACAGTCATCCAGTCCGTTTCCATCTGCGAAGACTGCATGATCGGCGCCGG
CTCCACGGTGGTGAGGGATATAACGGTTCCGGGAACCTATGTGGGGAGCCCGGCCAGGAGGATCAAGTGA

Upstream 100 bases:

>100_bases
ATAGAAAAGGCGTTCCAGTTGCTGGAGGCGACCCTGAGCATCCCGTGCAGCGCCAACCTGAGCGAAAGCGACATCGACTT
CGTCGCCGAAAGGCTGCGCC

Downstream 100 bases:

>100_bases
GAACGGGGAGGAGGCACACCGATGCCTGACGCATGTATCGCGGTGATTCCGGCGCGGGGAGGCTCCAAGCGGGTTCCCGG
GAAAAACATCAAGCCGCTCA

Product: hexapeptide transferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MASIVVIGGGGHAKVVISVLKKAGCLVAGYTDRQDRGPVLGIPWLGGDGVLPGLLGTHPTCGAIIGIGKVDASQLRLTLQ
DDISGLGFDFPVIVSPHAVVNEDVALGAGTVVLDGVVVNSGTETGRACILNTNSTVEHDCRLGDNVHIAPGVTLSGGVAV
GHNTMVGTGATVIQSVSICEDCMIGAGSTVVRDITVPGTYVGSPARRIK

Sequences:

>Translated_209_residues
MASIVVIGGGGHAKVVISVLKKAGCLVAGYTDRQDRGPVLGIPWLGGDGVLPGLLGTHPTCGAIIGIGKVDASQLRLTLQ
DDISGLGFDFPVIVSPHAVVNEDVALGAGTVVLDGVVVNSGTETGRACILNTNSTVEHDCRLGDNVHIAPGVTLSGGVAV
GHNTMVGTGATVIQSVSICEDCMIGAGSTVVRDITVPGTYVGSPARRIK
>Mature_208_residues
ASIVVIGGGGHAKVVISVLKKAGCLVAGYTDRQDRGPVLGIPWLGGDGVLPGLLGTHPTCGAIIGIGKVDASQLRLTLQD
DISGLGFDFPVIVSPHAVVNEDVALGAGTVVLDGVVVNSGTETGRACILNTNSTVEHDCRLGDNVHIAPGVTLSGGVAVG
HNTMVGTGATVIQSVSICEDCMIGAGSTVVRDITVPGTYVGSPARRIK

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 20997; Mature: 20866

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIVVIGGGGHAKVVISVLKKAGCLVAGYTDRQDRGPVLGIPWLGGDGVLPGLLGTHPT
CCEEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCCCCCHHHCCCCC
CGAIIGIGKVDASQLRLTLQDDISGLGFDFPVIVSPHAVVNEDVALGAGTVVLDGVVVNS
CCEEEEECCCCHHHEEEEEECCCCCCCCCCCEEECCCHHCCCCEECCCCEEEEEEEEEEC
GTETGRACILNTNSTVEHDCRLGDNVHIAPGVTLSGGVAVGHNTMVGTGATVIQSVSICE
CCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEECCEEECCCEEEECCHHHHHHHHHHH
DCMIGAGSTVVRDITVPGTYVGSPARRIK
HHHCCCCCEEEEEEECCCCCCCCCHHHCC
>Mature Secondary Structure 
ASIVVIGGGGHAKVVISVLKKAGCLVAGYTDRQDRGPVLGIPWLGGDGVLPGLLGTHPT
CEEEEEECCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCCCCCHHHCCCCC
CGAIIGIGKVDASQLRLTLQDDISGLGFDFPVIVSPHAVVNEDVALGAGTVVLDGVVVNS
CCEEEEECCCCHHHEEEEEECCCCCCCCCCCEEECCCHHCCCCEECCCCEEEEEEEEEEC
GTETGRACILNTNSTVEHDCRLGDNVHIAPGVTLSGGVAVGHNTMVGTGATVIQSVSICE
CCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEECCEEECCCEEEECCHHHHHHHHHHH
DCMIGAGSTVVRDITVPGTYVGSPARRIK
HHHCCCCCEEEEEEECCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]