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The map label for this gene is rpfG [H]

Identifier: 39996989

GI number: 39996989

Start: 2068572

End: 2069711

Strand: Reverse

Name: rpfG [H]

Synonym: GSU1891

Alternate gene names: 39996989

Gene position: 2069711-2068572 (Counterclockwise)

Preceding gene: 39996990

Following gene: 39996988

Centisome position: 54.26

GC content: 55.79

Gene sequence:

>1140_bases
ATGCTAAAGCGACACGATGGAACCATAGTCGTCATTGATGACGATCCCTACGTGCTCGAAAGCCTGTCGGCGCTTCTTGT
TGCCTACGGCTACATCGTGCATCCTTTCGGCCGGGGGGCCGATGCCGTGCATCGTCTCTCCTGCGGCGGTATCGATATTG
TCATTACCGATATCAATATGCCCGAGATGACGGGGATCGAAGTCCTGAGGGCTATTCGGAAGTCCAATCCGGATGTTCCG
GTGATCCTTATGACCGGTTATGCTGAATTGTCCGTGGCTGTGGAAGCGATCAAGCAGGGCGCTTTCGACTTCATTATCAA
GCCCTACGATCCGCTCTACCTTTTTCACGCCGTGGAGAAGGGGATCAATTATTACCGGCTGCTTAAGATCGAAAAGAATT
ACAAGGCCGAACTGGAAAACACAGTCAAGCAGCGGACGGGCGAACTGGCCGATACCCTTCAGATGCTCAAAAACATGAGC
AAGGAGGTTGTGCAGCGTCTCACCGCTGCGGCGGAACTTCGCGACGAGGACACCGGCGCCCACATTTCGCGGATCGGACT
CTATGCCAATCATATGGCCCGGGAGCTGGCCATGAGCGATGAATTCATCGATACAATCACGCTCGCGAGCGCCATGCACG
ATATCGGCAAGATCGGCATTCCCGACTCGATTCTCTTTAAGCCGGGACCGCTTTCCAGTGAAGAGTTCGCGACAATTCGC
ACTCATACGGTAATCGGTGCGCGCATCCTTCAGGGCTCATCCTACGGCATGCTCCGGATGGCCGAGTCGATTGCCCTTTG
TCACCATGAACGGTGGGACGGAACGGGCTATCCCTATGGGGTGAAGGGGGAGAATATCCCCGTGGAAGGACGTATTGTCA
TGCTCGTGGATCAGTATGATGCTCTGCGCAGCCCCCGGGTCTACAAGCCGCCGTATGACCATGAAACCGCGTGCAGGATC
ATCCTGGAGGGCGATGGCCGGACCATGCCGGAGCACTTCGATCCGGCGATACTCAGGGCCTTTCGAAACATCGCCGGCCG
TTTTGATGAGATATTCGAGGGGGAAAGTCACTCTGGTGAGGGCGGGACCGCAGGGGCTTCTGCGGCTACTGTCGCGGATT
TACGGAATATGAGCAATTAG

Upstream 100 bases:

>100_bases
TCTGTTTTGTCCGAAAAGAGAGTATCTCCCTTCATCATCACCGTGTTCTCCGAAAGGGTGGCACCGGAGCATGTCCATCC
TTTCGTAAGGAGCAATCACC

Downstream 100 bases:

>100_bases
TACTACAATCTTTGTGCCAAAACTAACTCAAAATATTGATATTCCTTTACAGGGTAAGGGGGAGATGGTATACTGCCCCT
GTTTTTATGGTAAAGACCAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLKRHDGTIVVIDDDPYVLESLSALLVAYGYIVHPFGRGADAVHRLSCGGIDIVITDINMPEMTGIEVLRAIRKSNPDVP
VILMTGYAELSVAVEAIKQGAFDFIIKPYDPLYLFHAVEKGINYYRLLKIEKNYKAELENTVKQRTGELADTLQMLKNMS
KEVVQRLTAAAELRDEDTGAHISRIGLYANHMARELAMSDEFIDTITLASAMHDIGKIGIPDSILFKPGPLSSEEFATIR
THTVIGARILQGSSYGMLRMAESIALCHHERWDGTGYPYGVKGENIPVEGRIVMLVDQYDALRSPRVYKPPYDHETACRI
ILEGDGRTMPEHFDPAILRAFRNIAGRFDEIFEGESHSGEGGTAGASAATVADLRNMSN

Sequences:

>Translated_379_residues
MLKRHDGTIVVIDDDPYVLESLSALLVAYGYIVHPFGRGADAVHRLSCGGIDIVITDINMPEMTGIEVLRAIRKSNPDVP
VILMTGYAELSVAVEAIKQGAFDFIIKPYDPLYLFHAVEKGINYYRLLKIEKNYKAELENTVKQRTGELADTLQMLKNMS
KEVVQRLTAAAELRDEDTGAHISRIGLYANHMARELAMSDEFIDTITLASAMHDIGKIGIPDSILFKPGPLSSEEFATIR
THTVIGARILQGSSYGMLRMAESIALCHHERWDGTGYPYGVKGENIPVEGRIVMLVDQYDALRSPRVYKPPYDHETACRI
ILEGDGRTMPEHFDPAILRAFRNIAGRFDEIFEGESHSGEGGTAGASAATVADLRNMSN
>Mature_379_residues
MLKRHDGTIVVIDDDPYVLESLSALLVAYGYIVHPFGRGADAVHRLSCGGIDIVITDINMPEMTGIEVLRAIRKSNPDVP
VILMTGYAELSVAVEAIKQGAFDFIIKPYDPLYLFHAVEKGINYYRLLKIEKNYKAELENTVKQRTGELADTLQMLKNMS
KEVVQRLTAAAELRDEDTGAHISRIGLYANHMARELAMSDEFIDTITLASAMHDIGKIGIPDSILFKPGPLSSEEFATIR
THTVIGARILQGSSYGMLRMAESIALCHHERWDGTGYPYGVKGENIPVEGRIVMLVDQYDALRSPRVYKPPYDHETACRI
ILEGDGRTMPEHFDPAILRAFRNIAGRFDEIFEGESHSGEGGTAGASAATVADLRNMSN

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=38.8429752066116, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=34.1085271317829, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=39.6694214876033, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788905, Length=114, Percent_Identity=35.0877192982456, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786784, Length=101, Percent_Identity=26.7326732673267, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41927; Mature: 41927

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRHDGTIVVIDDDPYVLESLSALLVAYGYIVHPFGRGADAVHRLSCGGIDIVITDINM
CCEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCC
PEMTGIEVLRAIRKSNPDVPVILMTGYAELSVAVEAIKQGAFDFIIKPYDPLYLFHAVEK
CCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
GINYYRLLKIEKNYKAELENTVKQRTGELADTLQMLKNMSKEVVQRLTAAAELRDEDTGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HISRIGLYANHMARELAMSDEFIDTITLASAMHDIGKIGIPDSILFKPGPLSSEEFATIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHEEH
THTVIGARILQGSSYGMLRMAESIALCHHERWDGTGYPYGVKGENIPVEGRIVMLVDQYD
HHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHH
ALRSPRVYKPPYDHETACRIILEGDGRTMPEHFDPAILRAFRNIAGRFDEIFEGESHSGE
HHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GGTAGASAATVADLRNMSN
CCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKRHDGTIVVIDDDPYVLESLSALLVAYGYIVHPFGRGADAVHRLSCGGIDIVITDINM
CCEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCC
PEMTGIEVLRAIRKSNPDVPVILMTGYAELSVAVEAIKQGAFDFIIKPYDPLYLFHAVEK
CCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
GINYYRLLKIEKNYKAELENTVKQRTGELADTLQMLKNMSKEVVQRLTAAAELRDEDTGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
HISRIGLYANHMARELAMSDEFIDTITLASAMHDIGKIGIPDSILFKPGPLSSEEFATIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHEEH
THTVIGARILQGSSYGMLRMAESIALCHHERWDGTGYPYGVKGENIPVEGRIVMLVDQYD
HHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHH
ALRSPRVYKPPYDHETACRIILEGDGRTMPEHFDPAILRAFRNIAGRFDEIFEGESHSGE
HHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GGTAGASAATVADLRNMSN
CCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]