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The map label for this gene is yjbJ [H]

Identifier: 39996925

GI number: 39996925

Start: 1993756

End: 1994364

Strand: Reverse

Name: yjbJ [H]

Synonym: GSU1826

Alternate gene names: 39996925

Gene position: 1994364-1993756 (Counterclockwise)

Preceding gene: 39996929

Following gene: 39996924

Centisome position: 52.29

GC content: 56.49

Gene sequence:

>609_bases
ATGACAAAGACGTTGACGATCATGTTGCTGGCAGGGGCGATGATCTGTTCTGCAGCAGTCGCGCCGGCCCGCGCCGACAT
CTACCGTTACGAGGATGAAGAGGGGGTCGTCCATTTTACCGACGCCCCCACCGATAAGCGCTTCAAGGTCTTCATGCGCG
ACATCAAGCGCGACAAGAAGCTCCGGACAACCTTTCGGCTGGCTAATTGCGCCCGCGATCCCAAGGAGTTCGAGCCGATT
ATCGATCAGTGCGCCCTTGAATTCGGTGTCGACCGTTCCCTGGTAAAAGCGGTTATTCACGCTGAATCGGGCTATAATCC
CAATGCTTTGTCCCCCAAGGGGGCCAGCGGCCTGATGCAGCTGATGCCGAAAACAGCCAAGGACCTGAAGGTTGCCAACT
CCTTTGACCCCAAGGATAACATCAAGGGGGGAGTGCGTTATCTCCGCTTCCTCCTTGATACATTCAAGGGGGATGTGACC
CTGGCCCTAGCCGCTTACAATGCCGGCATGTCGAGGGTTGCCCAGTACGGAGGCGTCCCCCCGTACCAGGAAACCCGCAC
CTATATTGATCGGGTGCTCAGTTACCAGAAATCCTACCAGACGAATTGA

Upstream 100 bases:

>100_bases
GCGATGTGCGGCTCCCTTTGGGTTGCATTGGCAATCGCGTTGGTCTATATTTTGAATGGCTTTACGCAGCGGCGCATGCC
ATAACCGCTGTGGAGGTGTA

Downstream 100 bases:

>100_bases
GACAGCCAATGGCCCCCGAATCCGCCAGCACCATCTGGAACGTGCCCAATATCCTGACCCTTGTCCGAATTGCCCTGATT
CCGGTCATGGCGGTACTGTT

Product: lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTKTLTIMLLAGAMICSAAVAPARADIYRYEDEEGVVHFTDAPTDKRFKVFMRDIKRDKKLRTTFRLANCARDPKEFEPI
IDQCALEFGVDRSLVKAVIHAESGYNPNALSPKGASGLMQLMPKTAKDLKVANSFDPKDNIKGGVRYLRFLLDTFKGDVT
LALAAYNAGMSRVAQYGGVPPYQETRTYIDRVLSYQKSYQTN

Sequences:

>Translated_202_residues
MTKTLTIMLLAGAMICSAAVAPARADIYRYEDEEGVVHFTDAPTDKRFKVFMRDIKRDKKLRTTFRLANCARDPKEFEPI
IDQCALEFGVDRSLVKAVIHAESGYNPNALSPKGASGLMQLMPKTAKDLKVANSFDPKDNIKGGVRYLRFLLDTFKGDVT
LALAAYNAGMSRVAQYGGVPPYQETRTYIDRVLSYQKSYQTN
>Mature_201_residues
TKTLTIMLLAGAMICSAAVAPARADIYRYEDEEGVVHFTDAPTDKRFKVFMRDIKRDKKLRTTFRLANCARDPKEFEPII
DQCALEFGVDRSLVKAVIHAESGYNPNALSPKGASGLMQLMPKTAKDLKVANSFDPKDNIKGGVRYLRFLLDTFKGDVTL
ALAAYNAGMSRVAQYGGVPPYQETRTYIDRVLSYQKSYQTN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=134, Percent_Identity=33.5820895522388, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22589; Mature: 22458

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLTIMLLAGAMICSAAVAPARADIYRYEDEEGVVHFTDAPTDKRFKVFMRDIKRDKK
CCCHHHHHHHHHHHHHHHHHCHHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHH
LRTTFRLANCARDPKEFEPIIDQCALEFGVDRSLVKAVIHAESGYNPNALSPKGASGLMQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LMPKTAKDLKVANSFDPKDNIKGGVRYLRFLLDTFKGDVTLALAAYNAGMSRVAQYGGVP
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCC
PYQETRTYIDRVLSYQKSYQTN
CHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKTLTIMLLAGAMICSAAVAPARADIYRYEDEEGVVHFTDAPTDKRFKVFMRDIKRDKK
CCHHHHHHHHHHHHHHHHHCHHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHH
LRTTFRLANCARDPKEFEPIIDQCALEFGVDRSLVKAVIHAESGYNPNALSPKGASGLMQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LMPKTAKDLKVANSFDPKDNIKGGVRYLRFLLDTFKGDVTLALAAYNAGMSRVAQYGGVP
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCC
PYQETRTYIDRVLSYQKSYQTN
CHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]