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The map label for this gene is tilS

Identifier: 39996909

GI number: 39996909

Start: 1975728

End: 1977143

Strand: Reverse

Name: tilS

Synonym: GSU1810

Alternate gene names: 39996909

Gene position: 1977143-1975728 (Counterclockwise)

Preceding gene: 39996910

Following gene: 39996908

Centisome position: 51.84

GC content: 59.53

Gene sequence:

>1416_bases
ATGAAAACGCTATCCCCGGTTGATGCGGTCTTGAAAAAAGTACGAGCCTTTAACGCTGAACACGGGATGTTCCGCCCGGG
GGACAGGGTTGTCGTTGCCGTATCGGGCGGTGCCGACTCGGTTGCGCTGGTGGATATCCTTGTTTCGCTTAACGAGTTGC
GGCTTGATCTGGTTGTTGCACATCTGAACCATAAGCTGCGTGGAGCGGCGTCTGACGAGGATGAGCGATTCGTGGAGCAG
TGCGCTCAGACATACGCCCTTCCGTTCGTAAGTCGGGGCGAGCAGGTAAAAGCACTAGCCGAATGGGAACGACTGTCCCT
CGAAGATGCCGGCCGACGGGCGAGGTATGCATTTTTTGATGACGTTGCCGCCGCGTGCGGAGCTCGAACCGTCGCTCTGG
CCCACCATGCCGACGACCAGGCTGAAACGCTGCTCATGCGGCTTGTGAGAGGGGCGGGAGGCCTGGGGTTGTCTGCCATG
CAACCCGTCGGGGCCGGCGGGAAGTATGTGAGACCCCTTCTTGCGCTGGGTCGACAGGAAATCGAGGCTTATCTCGATGC
GCGCGGACTGGCGTTCCGTCAGGATCAGAGCAATACCGACGTGGCCTTTCTGCGTAATCGTATCCGGCACGAACTCGTTC
CGTATCTGGGGCGGCTGAATCCGGGCGCCGCTTCCCGACTTGCCAGAACAGCCGAATTGCTTGCTGACGACGAAGCGTTA
CTGAGCCGACTCGTCGAAGAGGCATTGAGGCGGCATGTTATGGTAACGGAGCAGGGGGGGGCGATTTGCTCTGTCGACAC
CCTGCGCAGAGAACCGCGTGGACTTCGGCGTCGGCTGTACCGCAAGGCGATCGCGCTGGCCAAGGGTGATCTGGCGCGGA
TAAGCTTTGACCATGTGGATGCGATAGATCGGCTCGTGTTGTCAGAGAACCCATCCGGACGGCTCCATCTGCCCGAAGAA
ATTCTGGTAACCCGTTCCTACAATGAGGTTGCCTTTGCCCGGAGAATGGCTGACATCCCATCGCTTCAAGAACTGTGGAT
CGACGGTCCCGGCTGTTGGCCGCTGTCCGGCGGAGGGGAGCTCGTTGTCGAAATTGCTACGGGTCCTCCACCGTGGGGTG
AGCTTTCGACTACCGAGTCCTGGTTCGACCTGGAGCTGGCCCCGTTTCCCTGGCTTATCCGCGGTTGGAGGCCCGGCGAC
CGCATGATTCCCCTTGGCATGACCGGAGAGAAGAAGGTGAAGGACATCTTTATCGACCAAAAGGTTCCCCGCGAACTCCG
TCGAAGTATCCCGCTCTTATTCAGGGGAGATTCCCTTCTTTGGGTATGCGGCTGTCGTCGAGGAGAGTCTGCAAGGATAA
CAGCCGGCAGTCGGGCGATGGCTGTTGTGCGGATACGCGGCAGGCGCCTGAAATGA

Upstream 100 bases:

>100_bases
GGTTCAAGTCCCTGCACCGCCACCATTAACAATAACCCCTCCGGTCAGGAGGGGTTTTTTGTTTTGTGTCCCTACACGGC
TTTGACGCGAGACTCACTTC

Downstream 100 bases:

>100_bases
CCTGAAATCGCTTGTCAACCCGTTCCTTCTATGATAGTTTTTTTCAATTAGTGGTCTGAAACCAGTAACCATCAGCGTTC
GCGGGCGTTCGTCCATTGCC

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKTLSPVDAVLKKVRAFNAEHGMFRPGDRVVVAVSGGADSVALVDILVSLNELRLDLVVAHLNHKLRGAASDEDERFVEQ
CAQTYALPFVSRGEQVKALAEWERLSLEDAGRRARYAFFDDVAAACGARTVALAHHADDQAETLLMRLVRGAGGLGLSAM
QPVGAGGKYVRPLLALGRQEIEAYLDARGLAFRQDQSNTDVAFLRNRIRHELVPYLGRLNPGAASRLARTAELLADDEAL
LSRLVEEALRRHVMVTEQGGAICSVDTLRREPRGLRRRLYRKAIALAKGDLARISFDHVDAIDRLVLSENPSGRLHLPEE
ILVTRSYNEVAFARRMADIPSLQELWIDGPGCWPLSGGGELVVEIATGPPPWGELSTTESWFDLELAPFPWLIRGWRPGD
RMIPLGMTGEKKVKDIFIDQKVPRELRRSIPLLFRGDSLLWVCGCRRGESARITAGSRAMAVVRIRGRRLK

Sequences:

>Translated_471_residues
MKTLSPVDAVLKKVRAFNAEHGMFRPGDRVVVAVSGGADSVALVDILVSLNELRLDLVVAHLNHKLRGAASDEDERFVEQ
CAQTYALPFVSRGEQVKALAEWERLSLEDAGRRARYAFFDDVAAACGARTVALAHHADDQAETLLMRLVRGAGGLGLSAM
QPVGAGGKYVRPLLALGRQEIEAYLDARGLAFRQDQSNTDVAFLRNRIRHELVPYLGRLNPGAASRLARTAELLADDEAL
LSRLVEEALRRHVMVTEQGGAICSVDTLRREPRGLRRRLYRKAIALAKGDLARISFDHVDAIDRLVLSENPSGRLHLPEE
ILVTRSYNEVAFARRMADIPSLQELWIDGPGCWPLSGGGELVVEIATGPPPWGELSTTESWFDLELAPFPWLIRGWRPGD
RMIPLGMTGEKKVKDIFIDQKVPRELRRSIPLLFRGDSLLWVCGCRRGESARITAGSRAMAVVRIRGRRLK
>Mature_471_residues
MKTLSPVDAVLKKVRAFNAEHGMFRPGDRVVVAVSGGADSVALVDILVSLNELRLDLVVAHLNHKLRGAASDEDERFVEQ
CAQTYALPFVSRGEQVKALAEWERLSLEDAGRRARYAFFDDVAAACGARTVALAHHADDQAETLLMRLVRGAGGLGLSAM
QPVGAGGKYVRPLLALGRQEIEAYLDARGLAFRQDQSNTDVAFLRNRIRHELVPYLGRLNPGAASRLARTAELLADDEAL
LSRLVEEALRRHVMVTEQGGAICSVDTLRREPRGLRRRLYRKAIALAKGDLARISFDHVDAIDRLVLSENPSGRLHLPEE
ILVTRSYNEVAFARRMADIPSLQELWIDGPGCWPLSGGGELVVEIATGPPPWGELSTTESWFDLELAPFPWLIRGWRPGD
RMIPLGMTGEKKVKDIFIDQKVPRELRRSIPLLFRGDSLLWVCGCRRGESARITAGSRAMAVVRIRGRRLK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=215, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_GEOSL (Q74C65)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952860.1
- ProteinModelPortal:   Q74C65
- SMR:   Q74C65
- GeneID:   2686327
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1810
- NMPDR:   fig|243231.1.peg.1798
- TIGR:   GSU1810
- HOGENOM:   HBG668702
- OMA:   NDISYPL
- ProtClustDB:   CLSK828574
- BioCyc:   GSUL243231:GSU_1810-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 52163; Mature: 52163

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLSPVDAVLKKVRAFNAEHGMFRPGDRVVVAVSGGADSVALVDILVSLNELRLDLVVA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HLNHKLRGAASDEDERFVEQCAQTYALPFVSRGEQVKALAEWERLSLEDAGRRARYAFFD
HHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCHHHCCCCHHHHHHH
DVAAACGARTVALAHHADDQAETLLMRLVRGAGGLGLSAMQPVGAGGKYVRPLLALGRQE
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHCHHH
IEAYLDARGLAFRQDQSNTDVAFLRNRIRHELVPYLGRLNPGAASRLARTAELLADDEAL
HHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
LSRLVEEALRRHVMVTEQGGAICSVDTLRREPRGLRRRLYRKAIALAKGDLARISFDHVD
HHHHHHHHHHHCEEEEECCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHEEEHHHHH
AIDRLVLSENPSGRLHLPEEILVTRSYNEVAFARRMADIPSLQELWIDGPGCWPLSGGGE
HHHHHHHCCCCCCCEECCHHHEEECCCHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCCE
LVVEIATGPPPWGELSTTESWFDLELAPFPWLIRGWRPGDRMIPLGMTGEKKVKDIFIDQ
EEEEEECCCCCCCCCCCCCCEEEEEECCCCHHEECCCCCCCEEECCCCCCHHHHHHHCCC
KVPRELRRSIPLLFRGDSLLWVCGCRRGESARITAGSRAMAVVRIRGRRLK
CCHHHHHHCCCEEEECCCEEEEEECCCCCCCEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKTLSPVDAVLKKVRAFNAEHGMFRPGDRVVVAVSGGADSVALVDILVSLNELRLDLVVA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
HLNHKLRGAASDEDERFVEQCAQTYALPFVSRGEQVKALAEWERLSLEDAGRRARYAFFD
HHHHHHCCCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCHHHCCCCHHHHHHH
DVAAACGARTVALAHHADDQAETLLMRLVRGAGGLGLSAMQPVGAGGKYVRPLLALGRQE
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHCHHH
IEAYLDARGLAFRQDQSNTDVAFLRNRIRHELVPYLGRLNPGAASRLARTAELLADDEAL
HHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
LSRLVEEALRRHVMVTEQGGAICSVDTLRREPRGLRRRLYRKAIALAKGDLARISFDHVD
HHHHHHHHHHHCEEEEECCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCCCHHEEEHHHHH
AIDRLVLSENPSGRLHLPEEILVTRSYNEVAFARRMADIPSLQELWIDGPGCWPLSGGGE
HHHHHHHCCCCCCCEECCHHHEEECCCHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCCE
LVVEIATGPPPWGELSTTESWFDLELAPFPWLIRGWRPGDRMIPLGMTGEKKVKDIFIDQ
EEEEEECCCCCCCCCCCCCCEEEEEECCCCHHEECCCCCCCEEECCCCCCHHHHHHHCCC
KVPRELRRSIPLLFRGDSLLWVCGCRRGESARITAGSRAMAVVRIRGRRLK
CCHHHHHHCCCEEEECCCEEEEEECCCCCCCEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA