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The map label for this gene is tnpR [H]

Identifier: 39996810

GI number: 39996810

Start: 1876910

End: 1877482

Strand: Reverse

Name: tnpR [H]

Synonym: GSU1711

Alternate gene names: 39996810

Gene position: 1877482-1876910 (Counterclockwise)

Preceding gene: 39996812

Following gene: 39996800

Centisome position: 49.22

GC content: 55.67

Gene sequence:

>573_bases
ATGGCAATGATAGGTTATGCAAGGGTGAGCACTGAGGATCAAGACCTGACAGCACAGGTGAAGCAACTAACTGAGGCCGG
TTGTGATCGTCTCTACCGGGAAAAGGTGTCAGGTGTGAAGCGTGATCGACCTGAACTGGTGGCGATGTTGGATTACGTGC
GTGAGGGTGACGTTGTGGTGGTCTGCAAGCTGGACAGGATCGCCAGGAGTACGGCACACCTTCTTGAGATAACCGAGACG
CTAGAGAGGAAGGGCGTAGCCTTTCGGGTGTTGAACCTGAACCTTGATACCACAACGCCCACAGGCAAGCTCATGTTGAC
AATGTTGGGGGCAATCGCCACGTTTGAACGGGAGATGATGCTTGAGAGGCAACGGGAAGGGATCGCCACGGCGAAGGCAG
AGGGACGCTACACAGGGCGGAAGGCCACGGCGAAGGCAAAGGCGGGGGAAGTGTTGAGCCTCATTGCACAAGGCAAGGGA
AAGCAGACCGTAGCGGATGAACTTGGGATCGGGGTAGCATCGGTTTACCGGATCATTCAGGAGCACAAGGGTTCGGTTGG
CAATGCCTGTTGA

Upstream 100 bases:

>100_bases
TATGGGTATGGTTGAAATAGTCTATCATAAGGGTTGACATTGGGTTTTTGATAGAATATTATCGAAACATCTTTAACTCT
ATTGATAGGAGGTGTGGGCC

Downstream 100 bases:

>100_bases
CTGGAGAACGTAAAGAGGGAGATTCTTTAACCCTGGCCCGTGTGTTGCCCTCACATTGTCGGACAAGCGGCCTGGAGATT
CTCCCTTATGTTTCCTTATG

Product: integrative genetic element Gsu5, resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAMIGYARVSTEDQDLTAQVKQLTEAGCDRLYREKVSGVKRDRPELVAMLDYVREGDVVVVCKLDRIARSTAHLLEITET
LERKGVAFRVLNLNLDTTTPTGKLMLTMLGAIATFEREMMLERQREGIATAKAEGRYTGRKATAKAKAGEVLSLIAQGKG
KQTVADELGIGVASVYRIIQEHKGSVGNAC

Sequences:

>Translated_190_residues
MAMIGYARVSTEDQDLTAQVKQLTEAGCDRLYREKVSGVKRDRPELVAMLDYVREGDVVVVCKLDRIARSTAHLLEITET
LERKGVAFRVLNLNLDTTTPTGKLMLTMLGAIATFEREMMLERQREGIATAKAEGRYTGRKATAKAKAGEVLSLIAQGKG
KQTVADELGIGVASVYRIIQEHKGSVGNAC
>Mature_189_residues
AMIGYARVSTEDQDLTAQVKQLTEAGCDRLYREKVSGVKRDRPELVAMLDYVREGDVVVVCKLDRIARSTAHLLEITETL
ERKGVAFRVLNLNLDTTTPTGKLMLTMLGAIATFEREMMLERQREGIATAKAEGRYTGRKATAKAKAGEVLSLIAQGKGK
QTVADELGIGVASVYRIIQEHKGSVGNAC

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=179, Percent_Identity=41.340782122905, Blast_Score=140, Evalue=4e-35,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=29.5081967213115, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=29.5081967213115, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20787; Mature: 20656

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMIGYARVSTEDQDLTAQVKQLTEAGCDRLYREKVSGVKRDRPELVAMLDYVREGDVVV
CCEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEE
VCKLDRIARSTAHLLEITETLERKGVAFRVLNLNLDTTTPTGKLMLTMLGAIATFEREMM
EEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LERQREGIATAKAEGRYTGRKATAKAKAGEVLSLIAQGKGKQTVADELGIGVASVYRIIQ
HHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHH
EHKGSVGNAC
HHCCCCCCCC
>Mature Secondary Structure 
AMIGYARVSTEDQDLTAQVKQLTEAGCDRLYREKVSGVKRDRPELVAMLDYVREGDVVV
CEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEE
VCKLDRIARSTAHLLEITETLERKGVAFRVLNLNLDTTTPTGKLMLTMLGAIATFEREMM
EEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LERQREGIATAKAEGRYTGRKATAKAKAGEVLSLIAQGKGKQTVADELGIGVASVYRIIQ
HHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHH
EHKGSVGNAC
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2997130 [H]