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The map label for this gene is dosC [H]

Identifier: 39996758

GI number: 39996758

Start: 1819776

End: 1821152

Strand: Direct

Name: dosC [H]

Synonym: GSU1658

Alternate gene names: 39996758

Gene position: 1819776-1821152 (Clockwise)

Preceding gene: 39996757

Following gene: 39996759

Centisome position: 47.71

GC content: 54.83

Gene sequence:

>1377_bases
ATGGAACGGATTCTCGTTGTCGAAGATGACCGTTTTTTTCGTCAGATGTATGTTGATCTCCTGAAAGAGGAGGGATACGA
GGTCGATACCGTGGCATCGGGCACCGAGGGGTTGAAGCGGCTTGAGAAGCAAGAATACCACCTCGTCATTACCGACCTGG
TCATGCCCGGAATGAGCGGTATCGAGGTGTTGTCCCGCGTCAAGCAGAAAGCTCCGAACGTCGATGTCATCCTCGTCACC
GGTCACGCCAACCTCGAATCGGCCGTCTATGCCCTCAAGAATGGTGCCCGCGATTATATTCTCAAACCGTTCAACCATGA
TGAATTCAAGCACACCGTGGCACTTTGCTTTGAGCAGCGGAGGCTTATCAACGAAAACTACGAGCTCAAGGAGCTGCTGA
ATCTTTTTCAAGTTGGGCAGAACATAGCCAACTGTATCGACTTGGAACGGCTCTCTGCGGTTGTGGTCGATGCTTTCTGC
AAGGAGGTCGGAGTTTCACGCGCTATCGGCCTCTTTCCCGAAAAGAGCGAACCCCACGCCCTCAAGGAGCTGAGGGGGCT
TGAGCCTGAAGTTGCAGCCGCTCTTGCCGAAAAAGCTCTTACCCTTTGCAGTGACGCCGCGGAGACGGCAGGGGGCTTTC
GACGGCTCGACGGTTCCCATTTTTCCGATGGTCTCCTGCGAACTGCGGGGATTAATGGCGCCCTTGTGGTTAGCATCCGC
CAGCGTACGCTCCTGCAGGGAGTGCTTCTGCTGGTCAATGACCAGGGCAAGCCGTTCCCTGCCGTGTTCAAACATAAAAG
CATCCAGTTTTTGCTGGAGCAGGCATCGCTTGCCTTCGACAACGCCCTGCGTTACTCCAGCGCCCGCGACATGCTCTATG
TTGACGAACTCACGGGACTCTTCAACTACCGTTACCTTGACATCTCGCTGGACCGGGAGTTGAAGCGGGCTGACCGATTC
GGCTCGGTAGTTTCCATGATCTTCATCGACATGGACCACTTCAAGGGAGTCAACGACACCCACGGCCATCTTTTTGGGAG
CCAGGTCCTCCATGAAGTAGGTCAATTGCTCAAGAAGTCGGTCCGTGAGGTCGATGTAATCATTCGCTACGGTGGCGACG
AGTTCACCATAATTCTGGTGGAAACCGGTGAAAAGGGCGCTGCAACCGTGGCTGAAAGGATTCGTCGCTCCATCGAGGAC
CACCACTTTCTGGCCTCTGAAGGGCTCGATGTCCGGCTCACCGCAAGTCTCGGCTACGCCTGTTATCCCCTTGACACCCA
GTCCAAAATGGAACTTCTCGAACTGGCGGACAAAGCCATGTATAGGGGCAAGGAAGAGGGCAAAAACCGTGTATTCCGGG
CAACGGCAATCCGTTGA

Upstream 100 bases:

>100_bases
GTGGGCGGTGGGTACCTTCGCGAAAGGGCATTTTCGTTGACAAGTGGACCGCATTATCTGATAGTCTTGGGAAGACTTGC
CACTACCCCGGGAGTTGTGC

Downstream 100 bases:

>100_bases
CCTTGAGTGTGTTTCAATGCAATTAAAGGAGTAGGTACGTACATGATTACAGGTATCAAGGGATTCAACGACATTCTTCC
CGGGGAAGTGGAGCGGTGGC

Product: response regulator/GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MERILVVEDDRFFRQMYVDLLKEEGYEVDTVASGTEGLKRLEKQEYHLVITDLVMPGMSGIEVLSRVKQKAPNVDVILVT
GHANLESAVYALKNGARDYILKPFNHDEFKHTVALCFEQRRLINENYELKELLNLFQVGQNIANCIDLERLSAVVVDAFC
KEVGVSRAIGLFPEKSEPHALKELRGLEPEVAAALAEKALTLCSDAAETAGGFRRLDGSHFSDGLLRTAGINGALVVSIR
QRTLLQGVLLLVNDQGKPFPAVFKHKSIQFLLEQASLAFDNALRYSSARDMLYVDELTGLFNYRYLDISLDRELKRADRF
GSVVSMIFIDMDHFKGVNDTHGHLFGSQVLHEVGQLLKKSVREVDVIIRYGGDEFTIILVETGEKGAATVAERIRRSIED
HHFLASEGLDVRLTASLGYACYPLDTQSKMELLELADKAMYRGKEEGKNRVFRATAIR

Sequences:

>Translated_458_residues
MERILVVEDDRFFRQMYVDLLKEEGYEVDTVASGTEGLKRLEKQEYHLVITDLVMPGMSGIEVLSRVKQKAPNVDVILVT
GHANLESAVYALKNGARDYILKPFNHDEFKHTVALCFEQRRLINENYELKELLNLFQVGQNIANCIDLERLSAVVVDAFC
KEVGVSRAIGLFPEKSEPHALKELRGLEPEVAAALAEKALTLCSDAAETAGGFRRLDGSHFSDGLLRTAGINGALVVSIR
QRTLLQGVLLLVNDQGKPFPAVFKHKSIQFLLEQASLAFDNALRYSSARDMLYVDELTGLFNYRYLDISLDRELKRADRF
GSVVSMIFIDMDHFKGVNDTHGHLFGSQVLHEVGQLLKKSVREVDVIIRYGGDEFTIILVETGEKGAATVAERIRRSIED
HHFLASEGLDVRLTASLGYACYPLDTQSKMELLELADKAMYRGKEEGKNRVFRATAIR
>Mature_458_residues
MERILVVEDDRFFRQMYVDLLKEEGYEVDTVASGTEGLKRLEKQEYHLVITDLVMPGMSGIEVLSRVKQKAPNVDVILVT
GHANLESAVYALKNGARDYILKPFNHDEFKHTVALCFEQRRLINENYELKELLNLFQVGQNIANCIDLERLSAVVVDAFC
KEVGVSRAIGLFPEKSEPHALKELRGLEPEVAAALAEKALTLCSDAAETAGGFRRLDGSHFSDGLLRTAGINGALVVSIR
QRTLLQGVLLLVNDQGKPFPAVFKHKSIQFLLEQASLAFDNALRYSSARDMLYVDELTGLFNYRYLDISLDRELKRADRF
GSVVSMIFIDMDHFKGVNDTHGHLFGSQVLHEVGQLLKKSVREVDVIIRYGGDEFTIILVETGEKGAATVAERIRRSIED
HHFLASEGLDVRLTASLGYACYPLDTQSKMELLELADKAMYRGKEEGKNRVFRATAIR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=40.5063291139241, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=36.4197530864198, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=33.1428571428571, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=33.5443037974684, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.3095238095238, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=33.7278106508876, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=34.7826086956522, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081974, Length=105, Percent_Identity=41.9047619047619, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=30.625, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=37.2727272727273, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=37.2727272727273, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082012, Length=130, Percent_Identity=32.3076923076923, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788550, Length=145, Percent_Identity=31.0344827586207, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=32.520325203252, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788905, Length=116, Percent_Identity=32.7586206896552, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.0975609756098, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790552, Length=107, Percent_Identity=34.5794392523364, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=30, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=31.6666666666667, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789402, Length=102, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790863, Length=106, Percent_Identity=35.8490566037736, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=118, Percent_Identity=33.0508474576271, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787056, Length=186, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=29.559748427673, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1786599, Length=109, Percent_Identity=31.1926605504587, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=149, Percent_Identity=28.1879194630872, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51400; Mature: 51400

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERILVVEDDRFFRQMYVDLLKEEGYEVDTVASGTEGLKRLEKQEYHLVITDLVMPGMSG
CCCEEEEECHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHEEEEEHHHCCCCHH
IEVLSRVKQKAPNVDVILVTGHANLESAVYALKNGARDYILKPFNHDEFKHTVALCFEQR
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHH
RLINENYELKELLNLFQVGQNIANCIDLERLSAVVVDAFCKEVGVSRAIGLFPEKSEPHA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHH
LKELRGLEPEVAAALAEKALTLCSDAAETAGGFRRLDGSHFSDGLLRTAGINGALVVSIR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHCCCCCEEEEEEH
QRTLLQGVLLLVNDQGKPFPAVFKHKSIQFLLEQASLAFDNALRYSSARDMLYVDELTGL
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
FNYRYLDISLDRELKRADRFGSVVSMIFIDMDHFKGVNDTHGHLFGSQVLHEVGQLLKKS
HCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VREVDVIIRYGGDEFTIILVETGEKGAATVAERIRRSIEDHHFLASEGLDVRLTASLGYA
HHHEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEE
CYPLDTQSKMELLELADKAMYRGKEEGKNRVFRATAIR
EECCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCC
>Mature Secondary Structure
MERILVVEDDRFFRQMYVDLLKEEGYEVDTVASGTEGLKRLEKQEYHLVITDLVMPGMSG
CCCEEEEECHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHEEEEEHHHCCCCHH
IEVLSRVKQKAPNVDVILVTGHANLESAVYALKNGARDYILKPFNHDEFKHTVALCFEQR
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHH
RLINENYELKELLNLFQVGQNIANCIDLERLSAVVVDAFCKEVGVSRAIGLFPEKSEPHA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHH
LKELRGLEPEVAAALAEKALTLCSDAAETAGGFRRLDGSHFSDGLLRTAGINGALVVSIR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHCCCCCEEEEEEH
QRTLLQGVLLLVNDQGKPFPAVFKHKSIQFLLEQASLAFDNALRYSSARDMLYVDELTGL
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
FNYRYLDISLDRELKRADRFGSVVSMIFIDMDHFKGVNDTHGHLFGSQVLHEVGQLLKKS
HCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VREVDVIIRYGGDEFTIILVETGEKGAATVAERIRRSIEDHHFLASEGLDVRLTASLGYA
HHHEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEE
CYPLDTQSKMELLELADKAMYRGKEEGKNRVFRATAIR
EECCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]