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The map label for this gene is lexA-2

Identifier: 39996717

GI number: 39996717

Start: 1770881

End: 1771492

Strand: Reverse

Name: lexA-2

Synonym: GSU1617

Alternate gene names: 39996717

Gene position: 1771492-1770881 (Counterclockwise)

Preceding gene: 39996718

Following gene: 39996716

Centisome position: 46.45

GC content: 57.03

Gene sequence:

>612_bases
ATGGAAACACTTACCTCTCGCCAGCGCACGGTGCTTGAGTTCATAACCGCCCACGTGGATCGCCACGGCTATCCTCCTAC
CATGCGGGAAATAGCCCGCCACCTGAACGTGAACGGCACACTGGGCGTTGCAAAACACCTTGAGGCTCTTGCCCGGAAAG
GCTATCTCCAGCGGGAACCGGGGAACTCGCGCGGCATAACTCTGACTGGGCAGACACGGCACACCCCTACCGTTTCGTTA
CCCGTGATCGGAGTCGTGCGGGCCGGGGTTCCCCAGATCGCCACAGAGGAAATCGAGGAGCACATATCGATTGATCAATC
CCTGGTCAAAGGAGGTGCCTTTTTTCTCAGAGTCAAGGGTGACTCCATGATCAACGCCGCGATCATCGAAGGGGACCTGG
CCCTGGTCCGCCCCCAGCAAACAGCGGAAAACCGCGACATCGTGGTAGCCATGATCGACGGCGAAGCAACACTCAAACGT
TTCTACCAAGGAGCGGACCATATCCGGCTTCAGCCCGAAAATCCCAATATGGCCCCCATCATAGTTCGGAGCGGGGTACA
GGAGGCATGGATAATCGGCAAAGTTGTGGGTATTTACCGGCGTATGGATTAA

Upstream 100 bases:

>100_bases
GTAGCCTGACAGCAATTCCCGCTCACTGTTTCACACCCTTGACACCTTTGTTTTTCCCATGATAGGTTGACATATGTCAA
CCAATAAGGAAGCCGTCCCC

Downstream 100 bases:

>100_bases
CACACAAACTCCTCAGTACACAGGTAAGTAAATATACTGGCACTCAAGCATGAGCAATCATCGAACCATTCTCCATCTGG
ACATGAACGCCTTTTTTGCC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
METLTSRQRTVLEFITAHVDRHGYPPTMREIARHLNVNGTLGVAKHLEALARKGYLQREPGNSRGITLTGQTRHTPTVSL
PVIGVVRAGVPQIATEEIEEHISIDQSLVKGGAFFLRVKGDSMINAAIIEGDLALVRPQQTAENRDIVVAMIDGEATLKR
FYQGADHIRLQPENPNMAPIIVRSGVQEAWIIGKVVGIYRRMD

Sequences:

>Translated_203_residues
METLTSRQRTVLEFITAHVDRHGYPPTMREIARHLNVNGTLGVAKHLEALARKGYLQREPGNSRGITLTGQTRHTPTVSL
PVIGVVRAGVPQIATEEIEEHISIDQSLVKGGAFFLRVKGDSMINAAIIEGDLALVRPQQTAENRDIVVAMIDGEATLKR
FYQGADHIRLQPENPNMAPIIVRSGVQEAWIIGKVVGIYRRMD
>Mature_203_residues
METLTSRQRTVLEFITAHVDRHGYPPTMREIARHLNVNGTLGVAKHLEALARKGYLQREPGNSRGITLTGQTRHTPTVSL
PVIGVVRAGVPQIATEEIEEHISIDQSLVKGGAFFLRVKGDSMINAAIIEGDLALVRPQQTAENRDIVVAMIDGEATLKR
FYQGADHIRLQPENPNMAPIIVRSGVQEAWIIGKVVGIYRRMD

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=204, Percent_Identity=40.6862745098039, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787431, Length=128, Percent_Identity=32.8125, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA2_GEOSL (P61609)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952668.1
- ProteinModelPortal:   P61609
- SMR:   P61609
- MEROPS:   S24.001
- GeneID:   2685264
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1617
- NMPDR:   fig|243231.1.peg.1606
- TIGR:   GSU1617
- HOGENOM:   HBG679610
- OMA:   VGVWRGY
- ProtClustDB:   CLSK827607
- BioCyc:   GSUL243231:GSU_1617-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22445; Mature: 22445

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: ACT_SITE 122-122 ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLTSRQRTVLEFITAHVDRHGYPPTMREIARHLNVNGTLGVAKHLEALARKGYLQREP
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
GNSRGITLTGQTRHTPTVSLPVIGVVRAGVPQIATEEIEEHISIDQSLVKGGAFFLRVKG
CCCCCEEEECCCCCCCEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DSMINAAIIEGDLALVRPQQTAENRDIVVAMIDGEATLKRFYQGADHIRLQPENPNMAPI
CCEEEEEEEECCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEECCCCCCCEE
IVRSGVQEAWIIGKVVGIYRRMD
EEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METLTSRQRTVLEFITAHVDRHGYPPTMREIARHLNVNGTLGVAKHLEALARKGYLQREP
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
GNSRGITLTGQTRHTPTVSLPVIGVVRAGVPQIATEEIEEHISIDQSLVKGGAFFLRVKG
CCCCCEEEECCCCCCCEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DSMINAAIIEGDLALVRPQQTAENRDIVVAMIDGEATLKRFYQGADHIRLQPENPNMAPI
CCEEEEEEEECCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCCEEEECCCCCCCEE
IVRSGVQEAWIIGKVVGIYRRMD
EEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA