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The map label for this gene is dut

Identifier: 39996695

GI number: 39996695

Start: 1749458

End: 1749907

Strand: Direct

Name: dut

Synonym: GSU1595

Alternate gene names: 39996695

Gene position: 1749458-1749907 (Clockwise)

Preceding gene: 39996694

Following gene: 39996696

Centisome position: 45.87

GC content: 59.11

Gene sequence:

>450_bases
ATGCAGCCTTGTCTGGTAAAGATTCGTCGAATACGGTCCGGTTCGGACCTCCCTCTTCCCCGCTATATGACTCCTCACGC
CGCTGGTATGGACCTGTGTGCCGATGTGGATGCGGATCTGGTGCTTGAACCGGGAGAACGGGCGCTTGTTCCCACCGGCA
TTGCCATAGCCCTGCCCGATGGTTTTGAGGCGCAGATCAGGCCACGAAGCGGTCTTGCCCTCAAGCACGGAATTGCCCTC
GTCAACTCGCCGGGCACCATTGATCCCGACTATCGGGGCGAGATCGGCGTAATCATTGTTAATCACGGTGCAGACGCCTT
TGTCGTAAGGCGAGGTGAACGGATCGCCCAGATGGTATTTGCGCCCTTTGTGCGGGCTGAACTTCTGGATGTGGATGAAC
TGGATGAGACCGCCCGGGGTGACGGGGGCTTCGGTCACACCGGGCGGTAA

Upstream 100 bases:

>100_bases
GCGAGATTTTCGACGAGCGCTATCTGACCCTCGCCCTCATGGGCAAGATTGACAGTGCTGCGTTTGATGTTTCGCGGCTT
GCGCTCTAGGCGGCCGGTAC

Downstream 100 bases:

>100_bases
TCCTGGAAAATAGAGTGTACTGCTGTCTCTGACGGAGATATGTCATGCTTCTCTCCATTAATCCCGACAATCCGCAGGCA
CGACTTATCTCCCACGTGGC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MQPCLVKIRRIRSGSDLPLPRYMTPHAAGMDLCADVDADLVLEPGERALVPTGIAIALPDGFEAQIRPRSGLALKHGIAL
VNSPGTIDPDYRGEIGVIIVNHGADAFVVRRGERIAQMVFAPFVRAELLDVDELDETARGDGGFGHTGR

Sequences:

>Translated_149_residues
MQPCLVKIRRIRSGSDLPLPRYMTPHAAGMDLCADVDADLVLEPGERALVPTGIAIALPDGFEAQIRPRSGLALKHGIAL
VNSPGTIDPDYRGEIGVIIVNHGADAFVVRRGERIAQMVFAPFVRAELLDVDELDETARGDGGFGHTGR
>Mature_149_residues
MQPCLVKIRRIRSGSDLPLPRYMTPHAAGMDLCADVDADLVLEPGERALVPTGIAIALPDGFEAQIRPRSGLALKHGIAL
VNSPGTIDPDYRGEIGVIIVNHGADAFVVRRGERIAQMVFAPFVRAELLDVDELDETARGDGGFGHTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=144, Percent_Identity=41.6666666666667, Blast_Score=106, Evalue=6e-24,
Organism=Homo sapiens, GI4503423, Length=144, Percent_Identity=41.6666666666667, Blast_Score=106, Evalue=8e-24,
Organism=Homo sapiens, GI70906441, Length=144, Percent_Identity=41.6666666666667, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=48.6666666666667, Blast_Score=138, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71988561, Length=145, Percent_Identity=42.0689655172414, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319729, Length=141, Percent_Identity=38.2978723404255, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24583610, Length=148, Percent_Identity=34.4594594594595, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921126, Length=148, Percent_Identity=34.4594594594595, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_GEOSL (P61908)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952646.1
- ProteinModelPortal:   P61908
- SMR:   P61908
- GeneID:   2687289
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1595
- NMPDR:   fig|243231.1.peg.1584
- TIGR:   GSU1595
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- ProtClustDB:   PRK00601
- BioCyc:   GSUL243231:GSU_1595-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15993; Mature: 15993

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: BINDING 82-82

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPCLVKIRRIRSGSDLPLPRYMTPHAAGMDLCADVDADLVLEPGERALVPTGIAIALPD
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCEEECCCCCEEECCCEEEECCC
GFEAQIRPRSGLALKHGIALVNSPGTIDPDYRGEIGVIIVNHGADAFVVRRGERIAQMVF
CCCEEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHHHHHH
APFVRAELLDVDELDETARGDGGFGHTGR
HHHHHHHHCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQPCLVKIRRIRSGSDLPLPRYMTPHAAGMDLCADVDADLVLEPGERALVPTGIAIALPD
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCEEECCCCCEEECCCEEEECCC
GFEAQIRPRSGLALKHGIALVNSPGTIDPDYRGEIGVIIVNHGADAFVVRRGERIAQMVF
CCCEEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCHHHHHHHH
APFVRAELLDVDELDETARGDGGFGHTGR
HHHHHHHHCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA