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The map label for this gene is nusA [H]

Identifier: 39996686

GI number: 39996686

Start: 1738694

End: 1739851

Strand: Direct

Name: nusA [H]

Synonym: GSU1586

Alternate gene names: 39996686

Gene position: 1738694-1739851 (Clockwise)

Preceding gene: 39996685

Following gene: 39996687

Centisome position: 45.59

GC content: 58.64

Gene sequence:

>1158_bases
GTGGAAACGACCTTCAACCTCAAGCACATTATTGACCAGATCGTCAAGGAGAAGGGGATTGACCGGCACATCGTCGTGGA
AGCCCTGGAGCAGGCGGTACTCACCGCTGCGAACAAGAAGTTCCGCAATACTCGTGATCTTGAGGCCCACTATAACCCGG
AAGTTGGCGAAGTTGAGCTCTTTGAGTTCGTTACCGTGGTCGACGAGGTTCAGGATTCCTACAAGGAAATCGACATGGAA
GAGGCCCGGGAGATCGACCCTGACGTGGAAGTGGGCGATTCTCTCGGTATGAAGCTGGATGCAAGCGGTTTTACCCGTAT
CGCCGCCCAGACCGCCAAGCAGGTCATCATCCAGAAGGTGCGCGAGGCGGAGCGGGAAACTATTTTCAACGAGTTCAAGG
ACCGGATCGGCGAACTGGTGACCGGCGTTGTGCGCCGCTTTGAAAAAGGTGATCTGGTAATCGATCTCGGGCGCGCCGAA
GCGGTGCTTTCCCATAAGGAGCAGGCGCCGCGCGAGGTGTATCGCCAGGGTGACCGCGTTAAGACTCTGATCACCGACAT
CCGGATGACCCCAAAGGGGCCCCAGATCGTTCTGTCGCGTACCCATCCCGGCGTCCTTGCCAAGCTTTTCGAGGCGGAGG
TTCCGGAGATCGCCGAAGGGATCGTGGAGATCAAGGCCGTTGTACGTGAGCCGGGCAGCCGGGCCAAGATCGCCGTCTAC
TCCCATGATTCCGATGTGGATCCCGTTGGGGCCTGCGTGGGTATGCGGGGTAGCCGCGTGCAGAATGTGGTGTCCGAGCT
GAGGGGTGAAAAGATCGATATCATCCCCTGGTCCGATGACGCGGCACGCTTTGCGTGCAATGCGCTGCAACCGGCCGTGG
TGTCGAAGGTGTACATTGACGACGAGAACCGCTCCATGGAGATAATCGTCGCCGACGACCAACTGTCGCTGGCTATCGGT
AAAAAAGGGCAGAACGTGCGGCTTGCCGCAAAGCTTACCGGCTGGCGCATCGACATCAAGAGCGAAACCACTGCTGCCGA
GGCGGAACTGCTCCAGTATTCCTCCTATGATGGGGCCACCGAAGAGGTTGCTGAAGAGGCCGCCCAAGCCGTTGAGACCG
AAGGCGAAGCGGTTGCAGAGGAGCAGGTGGAAGCATAG

Upstream 100 bases:

>100_bases
ACGCTTCGGGAAGGGCAGCTGGCCCGCATCCCTCTGGATAAGATAGCTAAAGCTAACCTGGAATTCGAGTTTTAGAAAGA
TCAGCTCAAGGAGATTGGAC

Downstream 100 bases:

>100_bases
GTCATGGGAAAGGACGAACCGCGCAGATCCTGTATCGGTTGTCGTCAGGAGAGGGGCAAGGGCGAACTGCTCCGCTTCGT
TCTTGATCCCGAAGGAAGGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
METTFNLKHIIDQIVKEKGIDRHIVVEALEQAVLTAANKKFRNTRDLEAHYNPEVGEVELFEFVTVVDEVQDSYKEIDME
EAREIDPDVEVGDSLGMKLDASGFTRIAAQTAKQVIIQKVREAERETIFNEFKDRIGELVTGVVRRFEKGDLVIDLGRAE
AVLSHKEQAPREVYRQGDRVKTLITDIRMTPKGPQIVLSRTHPGVLAKLFEAEVPEIAEGIVEIKAVVREPGSRAKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSDDAARFACNALQPAVVSKVYIDDENRSMEIIVADDQLSLAIG
KKGQNVRLAAKLTGWRIDIKSETTAAEAELLQYSSYDGATEEVAEEAAQAVETEGEAVAEEQVEA

Sequences:

>Translated_385_residues
METTFNLKHIIDQIVKEKGIDRHIVVEALEQAVLTAANKKFRNTRDLEAHYNPEVGEVELFEFVTVVDEVQDSYKEIDME
EAREIDPDVEVGDSLGMKLDASGFTRIAAQTAKQVIIQKVREAERETIFNEFKDRIGELVTGVVRRFEKGDLVIDLGRAE
AVLSHKEQAPREVYRQGDRVKTLITDIRMTPKGPQIVLSRTHPGVLAKLFEAEVPEIAEGIVEIKAVVREPGSRAKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSDDAARFACNALQPAVVSKVYIDDENRSMEIIVADDQLSLAIG
KKGQNVRLAAKLTGWRIDIKSETTAAEAELLQYSSYDGATEEVAEEAAQAVETEGEAVAEEQVEA
>Mature_385_residues
METTFNLKHIIDQIVKEKGIDRHIVVEALEQAVLTAANKKFRNTRDLEAHYNPEVGEVELFEFVTVVDEVQDSYKEIDME
EAREIDPDVEVGDSLGMKLDASGFTRIAAQTAKQVIIQKVREAERETIFNEFKDRIGELVTGVVRRFEKGDLVIDLGRAE
AVLSHKEQAPREVYRQGDRVKTLITDIRMTPKGPQIVLSRTHPGVLAKLFEAEVPEIAEGIVEIKAVVREPGSRAKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSDDAARFACNALQPAVVSKVYIDDENRSMEIIVADDQLSLAIG
KKGQNVRLAAKLTGWRIDIKSETTAAEAELLQYSSYDGATEEVAEEAAQAVETEGEAVAEEQVEA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=333, Percent_Identity=44.1441441441441, Blast_Score=297, Evalue=9e-82,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42669; Mature: 42669

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTFNLKHIIDQIVKEKGIDRHIVVEALEQAVLTAANKKFRNTRDLEAHYNPEVGEVEL
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
FEFVTVVDEVQDSYKEIDMEEAREIDPDVEVGDSLGMKLDASGFTRIAAQTAKQVIIQKV
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
REAERETIFNEFKDRIGELVTGVVRRFEKGDLVIDLGRAEAVLSHKEQAPREVYRQGDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCHHHHHHCCCHH
KTLITDIRMTPKGPQIVLSRTHPGVLAKLFEAEVPEIAEGIVEIKAVVREPGSRAKIAVY
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSDDAARFACNALQPAVVSKVYID
ECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHEEEEEC
DENRSMEIIVADDQLSLAIGKKGQNVRLAAKLTGWRIDIKSETTAAEAELLQYSSYDGAT
CCCCEEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHCCCCCHH
EEVAEEAAQAVETEGEAVAEEQVEA
HHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
METTFNLKHIIDQIVKEKGIDRHIVVEALEQAVLTAANKKFRNTRDLEAHYNPEVGEVEL
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
FEFVTVVDEVQDSYKEIDMEEAREIDPDVEVGDSLGMKLDASGFTRIAAQTAKQVIIQKV
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHH
REAERETIFNEFKDRIGELVTGVVRRFEKGDLVIDLGRAEAVLSHKEQAPREVYRQGDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCHHHHHHCCCHH
KTLITDIRMTPKGPQIVLSRTHPGVLAKLFEAEVPEIAEGIVEIKAVVREPGSRAKIAVY
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSDDAARFACNALQPAVVSKVYID
ECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHEEEEEC
DENRSMEIIVADDQLSLAIGKKGQNVRLAAKLTGWRIDIKSETTAAEAELLQYSSYDGAT
CCCCEEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEECCCCHHHHHHHHHHCCCCCHH
EEVAEEAAQAVETEGEAVAEEQVEA
HHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]