Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 39996645

GI number: 39996645

Start: 1697747

End: 1698361

Strand: Direct

Name: rhtB [H]

Synonym: GSU1545

Alternate gene names: 39996645

Gene position: 1697747-1698361 (Clockwise)

Preceding gene: 39996644

Following gene: 39996646

Centisome position: 44.51

GC content: 62.28

Gene sequence:

>615_bases
ATGATCAGCGCCGAATTTCTCATCACCTCACTCATTGTCGTCCTCATTCCCGGTACCGGAGTCATTTTCACCGTTTCCAC
GGGGATCGCCCAGGGGCGCGCGGCCAGCGTCTACGCGGCGCTCGGCTGCACCGCCGGGATCATCCCGCATCTGCTGGCGA
CGATCCTGGGGCTTGCGGCGCTCATGCACACCAGCGCGCTGGCATTCCAGACCCTCAGGTACGCCGGGGTGGCGTATCTG
CTCTATGTGGCCTATGCCACGTGGAAAGACACCTCTACCTTTGCCCTGGACAGCACCCCTTCGCGACGCTCGGCGTTGTC
ACTGGTGGTGAGGGCGCTGCTGCTCAACATCCTGAATCCCAAACTGACCATCTTTTTCCTGGCCTTCCTGCCGCAATTCG
TCAGGCCCGGCCCGGCCGGCCCCCTGCCGCAGTTGCTGGTCCTGAGCGGCGTGTTCATGGCCATGACCTTTGCGGTGTTC
GTGGTCTATGGTCTGCTGGCCAATGCCTTTCGTACGGTTGTGATCGAATCGGAATCGGTCCAGGCCTGGTTGCGCCGAAG
CTTCGCCGGCGCGTTTGCCGCTCTGGGCGTGCATCTGGCTTTTTCGGAGAAGTGA

Upstream 100 bases:

>100_bases
TACTGATCCGACTGGATGCACGCCGGCCCGCAGCGTTGGGATGTTTGCCGGAAACCGTTTCTGAAGTGTCGAGACAACAC
CGATTCACACAAGGACAGCC

Downstream 100 bases:

>100_bases
AGTGGAAGGCTGATGGAGTGAGAGCCTGAGAATATTCCGCAGGGGAGTTCCGATCGTCTCGCAGGGCTTCTCTGCCATTT
ATCCGGCGCATATATGCTTC

Product: transporter, LysE family

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MISAEFLITSLIVVLIPGTGVIFTVSTGIAQGRAASVYAALGCTAGIIPHLLATILGLAALMHTSALAFQTLRYAGVAYL
LYVAYATWKDTSTFALDSTPSRRSALSLVVRALLLNILNPKLTIFFLAFLPQFVRPGPAGPLPQLLVLSGVFMAMTFAVF
VVYGLLANAFRTVVIESESVQAWLRRSFAGAFAALGVHLAFSEK

Sequences:

>Translated_204_residues
MISAEFLITSLIVVLIPGTGVIFTVSTGIAQGRAASVYAALGCTAGIIPHLLATILGLAALMHTSALAFQTLRYAGVAYL
LYVAYATWKDTSTFALDSTPSRRSALSLVVRALLLNILNPKLTIFFLAFLPQFVRPGPAGPLPQLLVLSGVFMAMTFAVF
VVYGLLANAFRTVVIESESVQAWLRRSFAGAFAALGVHLAFSEK
>Mature_204_residues
MISAEFLITSLIVVLIPGTGVIFTVSTGIAQGRAASVYAALGCTAGIIPHLLATILGLAALMHTSALAFQTLRYAGVAYL
LYVAYATWKDTSTFALDSTPSRRSALSLVVRALLLNILNPKLTIFFLAFLPQFVRPGPAGPLPQLLVLSGVFMAMTFAVF
VVYGLLANAFRTVVIESESVQAWLRRSFAGAFAALGVHLAFSEK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=195, Percent_Identity=31.2820512820513, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788099, Length=189, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21698; Mature: 21698

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAEFLITSLIVVLIPGTGVIFTVSTGIAQGRAASVYAALGCTAGIIPHLLATILGLAA
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMHTSALAFQTLRYAGVAYLLYVAYATWKDTSTFALDSTPSRRSALSLVVRALLLNILNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCH
KLTIFFLAFLPQFVRPGPAGPLPQLLVLSGVFMAMTFAVFVVYGLLANAFRTVVIESESV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QAWLRRSFAGAFAALGVHLAFSEK
HHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MISAEFLITSLIVVLIPGTGVIFTVSTGIAQGRAASVYAALGCTAGIIPHLLATILGLAA
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMHTSALAFQTLRYAGVAYLLYVAYATWKDTSTFALDSTPSRRSALSLVVRALLLNILNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCH
KLTIFFLAFLPQFVRPGPAGPLPQLLVLSGVFMAMTFAVFVVYGLLANAFRTVVIESESV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QAWLRRSFAGAFAALGVHLAFSEK
HHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]