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The map label for this gene is rluB [C]

Identifier: 39996504

GI number: 39996504

Start: 1544951

End: 1545694

Strand: Direct

Name: rluB [C]

Synonym: GSU1403

Alternate gene names: 39996504

Gene position: 1544951-1545694 (Clockwise)

Preceding gene: 308513299

Following gene: 39996506

Centisome position: 40.51

GC content: 66.67

Gene sequence:

>744_bases
ATGGAAGAACGATTGCAGAAGCTGCTGTCCCAGGCGGGGGTCGCGTCCCGGCGCGAGGCGGAACGGTTCATCACCGAGGG
ACGGGTGGCGGTGAACGGCACGGTCGTTACCGAGCTGGGGAGCAAGGCCGACCCGGAGCGGGACCGGATAACCGTGGACG
GGCAGCCCGTCCGTCCGGCGGAGAAGAAGGTGTATCTCATCCTCAACAAGCCCGTGGGCTATATGACCACCCTCAGGGAT
CCGGAGGGGAGACCCATTGTCACCGATCTCCTCAAGGGGCTCGATGTCCGCGTCTTTCCCGTGGGGCGGCTCGACTACAA
CACCGAGGGGCTTCTGCTGCTCACCAATGACGGTGCCTGGGCCAACCGGCTCGCCCATCCCCGGCATGAGGTGGACAAGG
AATACCTGGTCCGGGTGCGGGGGGCTGTGGCCAAGGAGCAGATCCGGCGGCTGGCTACCGGTGTGGAACTGGACGACGGG
CCGACGGCGCCGGCAAAGGTGGAGGTGTCCAGCCAGAGCGATAACAACACCTGGCTTTCCATCGTGATTCACGAGGGGCG
CTACCGCCAGGTGCGCCGCATGTGCGAAGCCGTGAGCCTGTCGGTGGTCCGCCTGCGCCGGGTGAGATACGGCGCGGTCT
CGCTCGGCGACCTGAAACCGGGGGAGTACCGGCCCCTCAGCCCGACGGAGGTTGCAGCCCTGGCCGGCCGCGAAAAACGA
GAACAGCGCCGTCGGAAAGCCTGA

Upstream 100 bases:

>100_bases
GGGGGCGGCAACCGCCTTCGGGCAACCTGACAGTCTGAAGGCTGGAGACGTTCGCGGGGAGTTCCCCGGTCTTCGGCCTT
TAGTCTTTTTGGAGGGGCAT

Downstream 100 bases:

>100_bases
TCGCTCAAGGCTGCTTTTGTTGGATCATTTTGTGTCCGGATTTAGTGCATCGTTTTGAGGGGAAATGCGCATGGCCTCAT
GCGCAGCAGAAATTTTCGGC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MEERLQKLLSQAGVASRREAERFITEGRVAVNGTVVTELGSKADPERDRITVDGQPVRPAEKKVYLILNKPVGYMTTLRD
PEGRPIVTDLLKGLDVRVFPVGRLDYNTEGLLLLTNDGAWANRLAHPRHEVDKEYLVRVRGAVAKEQIRRLATGVELDDG
PTAPAKVEVSSQSDNNTWLSIVIHEGRYRQVRRMCEAVSLSVVRLRRVRYGAVSLGDLKPGEYRPLSPTEVAALAGREKR
EQRRRKA

Sequences:

>Translated_247_residues
MEERLQKLLSQAGVASRREAERFITEGRVAVNGTVVTELGSKADPERDRITVDGQPVRPAEKKVYLILNKPVGYMTTLRD
PEGRPIVTDLLKGLDVRVFPVGRLDYNTEGLLLLTNDGAWANRLAHPRHEVDKEYLVRVRGAVAKEQIRRLATGVELDDG
PTAPAKVEVSSQSDNNTWLSIVIHEGRYRQVRRMCEAVSLSVVRLRRVRYGAVSLGDLKPGEYRPLSPTEVAALAGREKR
EQRRRKA
>Mature_247_residues
MEERLQKLLSQAGVASRREAERFITEGRVAVNGTVVTELGSKADPERDRITVDGQPVRPAEKKVYLILNKPVGYMTTLRD
PEGRPIVTDLLKGLDVRVFPVGRLDYNTEGLLLLTNDGAWANRLAHPRHEVDKEYLVRVRGAVAKEQIRRLATGVELDDG
PTAPAKVEVSSQSDNNTWLSIVIHEGRYRQVRRMCEAVSLSVVRLRRVRYGAVSLGDLKPGEYRPLSPTEVAALAGREKR
EQRRRKA

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=221, Percent_Identity=46.1538461538462, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=39.3162393162393, Blast_Score=153, Evalue=9e-39,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=36.9098712446352, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27725; Mature: 27725

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERLQKLLSQAGVASRREAERFITEGRVAVNGTVVTELGSKADPERDRITVDGQPVRPA
CHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCEEEECCCCCCCC
EKKVYLILNKPVGYMTTLRDPEGRPIVTDLLKGLDVRVFPVGRLDYNTEGLLLLTNDGAW
CCEEEEEEECCCCCEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCH
ANRLAHPRHEVDKEYLVRVRGAVAKEQIRRLATGVELDDGPTAPAKVEVSSQSDNNTWLS
HHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEE
IVIHEGRYRQVRRMCEAVSLSVVRLRRVRYGAVSLGDLKPGEYRPLSPTEVAALAGREKR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHCHHHH
EQRRRKA
HHHHCCC
>Mature Secondary Structure
MEERLQKLLSQAGVASRREAERFITEGRVAVNGTVVTELGSKADPERDRITVDGQPVRPA
CHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCEEEECCCCCCCC
EKKVYLILNKPVGYMTTLRDPEGRPIVTDLLKGLDVRVFPVGRLDYNTEGLLLLTNDGAW
CCEEEEEEECCCCCEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCH
ANRLAHPRHEVDKEYLVRVRGAVAKEQIRRLATGVELDDGPTAPAKVEVSSQSDNNTWLS
HHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEE
IVIHEGRYRQVRRMCEAVSLSVVRLRRVRYGAVSLGDLKPGEYRPLSPTEVAALAGREKR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHCHHHH
EQRRRKA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]