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The map label for this gene is tgt-1 [H]

Identifier: 39996277

GI number: 39996277

Start: 1280212

End: 1281318

Strand: Reverse

Name: tgt-1 [H]

Synonym: GSU1175

Alternate gene names: 39996277

Gene position: 1281318-1280212 (Counterclockwise)

Preceding gene: 39996283

Following gene: 39996276

Centisome position: 33.59

GC content: 59.62

Gene sequence:

>1107_bases
ATGAAATTCAAATTAATTAAAAAAGACACCAAGACATCTGCGCGGCGAGGATCACTCACGACCCCTCACGGCACCATCGA
AACCCCCATCTTCATGCCCGTCGGCACCCACGCGGCCATGAAAGCGATGACGCCCTCCCAGGTCAAAGAGACGGGAGCCC
AGATCATCCTTTCCAACACGTACCATCTCCACCTGCGCCCCGGCGAGGACCTTATTGCCAAGGCCGGGGGCCTGCACCGG
TTCATGGGATGGGACGGACCGATTCTGACCGATTCGGGCGGTTTTCAGGTCTTCTCGTTGCCCAACAAGAAGATTACCGA
CGAGGGGGTCTTCTTCCGCCACGAAGTGAGCGGCGAGGAAGTGTTCCTCGACCCGGCCCGGGCCACGGCGATCCAGGAAG
CGCTTGGCGCCGACATCATCATGGCTTTTGACGAGTGCATCCCCTACCCCTGCGACAAAAAGTACGCGGCGGCATCGACC
CGCAAGACCATCAGGTGGGCGGAGGCGTGCAAGAAAGCCCACACACGCAAGGACCAGGCACTGTTCGGCATCGTTCAGGG
GAGCGTATTCGAGGACCTGCGTGCCCAGTGCGCCAGGGAACTGGTCCAGCTCGACTTCCCCGGCTACGCAGTGGGAGGCG
TGTCGGTGGGCGAAGGTCTTGAACTGCTGAAAAAGGTAGTTGAGTACACGGCCCCGTTCCTCCCGGAGAACAAGCCCCGC
TACCTCATGGGGGTGGGACTGCCGGAGGATATCCTGGAGAGCGTGGAGCGGGGGATGGACATGTTCGACTGCGTGATACC
GACCCGCTATGCCCGGAGCGCTACCCTCTTCACCAACAGGGGGAAAATCCGCCTGACCCATCGTAACTACCGGCGCGACT
TCTACCCCGTCGACCCCAACTGCACTTGCTATACCTGCCGGAACTTCACCCGGGCGTATCTCCACCACCTCTTCAACGCC
AACGAGATACTCTCCGCAACCCTGGCGAGCATCCACAACGTGCACTTCTATCTGAACATGATGGCGGAGATACGCGCTGC
CATTGAAGAGGAGCGCTTTGCCGAGTATAAGCGGAACTTCCTGGAGAGCTACCTGAAGGGTAAATAG

Upstream 100 bases:

>100_bases
ATACAACCAAAGGCCGAACATCACGGAAAAGCAGCAACTTAGGCGTACAAAAAGCCCATATTCTCGCGCAATTGTAATAC
AACCTCCTCGAAAAGGCAAC

Downstream 100 bases:

>100_bases
GAAAGATTAAAGCGAACACATGCCAGGGCATCCGAACGGGTGCTCTTTTGCCTGGAGGAACGGACACCATGACGGACGAA
CAGGCCGGCTTCGGCAGAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKFKLIKKDTKTSARRGSLTTPHGTIETPIFMPVGTHAAMKAMTPSQVKETGAQIILSNTYHLHLRPGEDLIAKAGGLHR
FMGWDGPILTDSGGFQVFSLPNKKITDEGVFFRHEVSGEEVFLDPARATAIQEALGADIIMAFDECIPYPCDKKYAAAST
RKTIRWAEACKKAHTRKDQALFGIVQGSVFEDLRAQCARELVQLDFPGYAVGGVSVGEGLELLKKVVEYTAPFLPENKPR
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTHRNYRRDFYPVDPNCTCYTCRNFTRAYLHHLFNA
NEILSATLASIHNVHFYLNMMAEIRAAIEEERFAEYKRNFLESYLKGK

Sequences:

>Translated_368_residues
MKFKLIKKDTKTSARRGSLTTPHGTIETPIFMPVGTHAAMKAMTPSQVKETGAQIILSNTYHLHLRPGEDLIAKAGGLHR
FMGWDGPILTDSGGFQVFSLPNKKITDEGVFFRHEVSGEEVFLDPARATAIQEALGADIIMAFDECIPYPCDKKYAAAST
RKTIRWAEACKKAHTRKDQALFGIVQGSVFEDLRAQCARELVQLDFPGYAVGGVSVGEGLELLKKVVEYTAPFLPENKPR
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTHRNYRRDFYPVDPNCTCYTCRNFTRAYLHHLFNA
NEILSATLASIHNVHFYLNMMAEIRAAIEEERFAEYKRNFLESYLKGK
>Mature_368_residues
MKFKLIKKDTKTSARRGSLTTPHGTIETPIFMPVGTHAAMKAMTPSQVKETGAQIILSNTYHLHLRPGEDLIAKAGGLHR
FMGWDGPILTDSGGFQVFSLPNKKITDEGVFFRHEVSGEEVFLDPARATAIQEALGADIIMAFDECIPYPCDKKYAAAST
RKTIRWAEACKKAHTRKDQALFGIVQGSVFEDLRAQCARELVQLDFPGYAVGGVSVGEGLELLKKVVEYTAPFLPENKPR
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTHRNYRRDFYPVDPNCTCYTCRNFTRAYLHHLFNA
NEILSATLASIHNVHFYLNMMAEIRAAIEEERFAEYKRNFLESYLKGK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=43.4903047091413, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI13375872, Length=355, Percent_Identity=25.0704225352113, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=52.1739130434783, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17544680, Length=353, Percent_Identity=43.342776203966, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI25146502, Length=170, Percent_Identity=30, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920510, Length=370, Percent_Identity=41.6216216216216, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI21355311, Length=171, Percent_Identity=32.1637426900585, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41490; Mature: 41490

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKLIKKDTKTSARRGSLTTPHGTIETPIFMPVGTHAAMKAMTPSQVKETGAQIILSNT
CCEEEECCCCCCHHCCCCCCCCCCCCCCCEEEECCCHHHHHCCCHHHHHHCCCEEEEECE
YHLHLRPGEDLIAKAGGLHRFMGWDGPILTDSGGFQVFSLPNKKITDEGVFFRHEVSGEE
EEEEECCCHHHHHHCCCCHHHCCCCCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCCE
VFLDPARATAIQEALGADIIMAFDECIPYPCDKKYAAASTRKTIRWAEACKKAHTRKDQA
EEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
LFGIVQGSVFEDLRAQCARELVQLDFPGYAVGGVSVGEGLELLKKVVEYTAPFLPENKPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTHRNYRRDFYPVDPN
EEEECCCCHHHHHHHHHCCHHHHHHCCHHHCCCEEEEECCCEEEEEECCCCCCCCCCCCC
CTCYTCRNFTRAYLHHLFNANEILSATLASIHNVHFYLNMMAEIRAAIEEERFAEYKRNF
CEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESYLKGK
HHHHHCCC
>Mature Secondary Structure
MKFKLIKKDTKTSARRGSLTTPHGTIETPIFMPVGTHAAMKAMTPSQVKETGAQIILSNT
CCEEEECCCCCCHHCCCCCCCCCCCCCCCEEEECCCHHHHHCCCHHHHHHCCCEEEEECE
YHLHLRPGEDLIAKAGGLHRFMGWDGPILTDSGGFQVFSLPNKKITDEGVFFRHEVSGEE
EEEEECCCHHHHHHCCCCHHHCCCCCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCCE
VFLDPARATAIQEALGADIIMAFDECIPYPCDKKYAAASTRKTIRWAEACKKAHTRKDQA
EEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
LFGIVQGSVFEDLRAQCARELVQLDFPGYAVGGVSVGEGLELLKKVVEYTAPFLPENKPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCC
YLMGVGLPEDILESVERGMDMFDCVIPTRYARSATLFTNRGKIRLTHRNYRRDFYPVDPN
EEEECCCCHHHHHHHHHCCHHHHHHCCHHHCCCEEEEECCCEEEEEECCCCCCCCCCCCC
CTCYTCRNFTRAYLHHLFNANEILSATLASIHNVHFYLNMMAEIRAAIEEERFAEYKRNF
CEEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESYLKGK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA