Click here to switch to the map view.

The map label for this gene is macA [H]

Identifier: 39996263

GI number: 39996263

Start: 1265187

End: 1266401

Strand: Direct

Name: macA [H]

Synonym: GSU1161

Alternate gene names: 39996263

Gene position: 1265187-1266401 (Clockwise)

Preceding gene: 39996262

Following gene: 39996264

Centisome position: 33.17

GC content: 66.83

Gene sequence:

>1215_bases
ATGGGTCATGAGGATCTGCAGAAACTGAAAATCGACAAAGCGGCGGCACTCCGCCCCGCTGCCCGGCGCAGGCCCCTCTA
CTGGATCGGGGCGGCCGTTGCCCTTGCGCTGCTGCTGGTCCTGTTCGCAACCGGCGTTCTCACTCCGGCCGTGACCGTTG
AAACCGGCACGGTGACCCAGGTGTACCCGTCCCAGACCTTTACCCTGCTCAACGCCAGCGGATACGTGGTGGCCCAGCGT
AAGGCGGCCGTTGCTGCGAAGCTCACTGGCCGGCTGGTCTGGCTCGGCGTAGAGGAGGGGAGCAGGGTCCGCAAAGGAGA
TGTGCTGGCCCGGCTGGAGAGCGACGATGCCGCTGCCGGCCGGGATCAGGCGGCCGCCAACCTGGGCACGGCCCGGGCGA
GCCTGGACCAGGCCCGGGCCGAGCTGACCGATGCCAGCCAGGCCTATGAGCGGCAGAAGCAGCTCGTGCGCGAGGGGATC
GTGGCAAAGGCCGATTTTGACGCGGCAGAGGCCCGCTTCCTCCGGGCCCGGGCCGCAGTGACCGCCGCCGAGTCATCAAT
CCGCGCCGCCTCGGCCGCCCTGCGAGGGGCGGAGGTGAACCTGGAGTATTCCCTCATCCGCGCCCCCTTCGATGCCGTGG
TCCTGACCAAGAATGCGGACGTGGGCGACATCGTCACCCCCATCGGCGCCGCTGCCGACGCCAAGGCCGCCGTGGTCAGC
ATCGCAGACTTGGCATCGCTCCAGGTAGAAGCGGATGTTTCTGAATCCAACTTGGCCAAGGTCAAGGAGGGACAGCCGTG
CGAGATCCAGCTCGACTCTTTGCCGGATGAGCGCTTCCGGGGCTTCGTCCACATGATCGTCCCCACGGCTGATCGCTCCA
AGGCGACAGTTCTGGTGAAGGTCCGCTTTGAGAAACTTGACCCTCGTATCCTCCCCGAGATGAGCGCCAAGGTGGCATTT
TTGGAGCGGCCGGTGGCCGCGGGGGAAGAGAAGCCGAAGACCGCCGTGAATCCTGCCGCCGTGGCCGATCGGGACGGCCG
CAAGGTCCTTTTCGTTCTGAAGGGAGACCGGGTGGCCGCCGTGCCCGTGACCCTCGGCGCCCGCCAGGGAGACATGGTGG
AGGTTGTCTCCGGCGCCAAGGCCGGGGACAGGATCGTCGTCAAGCCCCTGGACAAGCTCAAGGACGGCACCCGCGTCAAA
ACGGCGGAGAAGTAG

Upstream 100 bases:

>100_bases
GGTCATCGAGGTGGTTGAGTAGATTGCCGACCGGTGGTATGGTGTTGCGATGCGCCGGCACCGCATCCGGCCGCCACTTT
CTCCGTTCGGAGTCCATGCC

Downstream 100 bases:

>100_bases
ATGAGCGAAACGCGGGAACCCATCGTCGCCATCCGCAATCTTTCCAAGTCCTACCGCCGGGGGGCGCAACTGATCCCGGT
CCTCCAGGACATTACCCTGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MGHEDLQKLKIDKAAALRPAARRRPLYWIGAAVALALLLVLFATGVLTPAVTVETGTVTQVYPSQTFTLLNASGYVVAQR
KAAVAAKLTGRLVWLGVEEGSRVRKGDVLARLESDDAAAGRDQAAANLGTARASLDQARAELTDASQAYERQKQLVREGI
VAKADFDAAEARFLRARAAVTAAESSIRAASAALRGAEVNLEYSLIRAPFDAVVLTKNADVGDIVTPIGAAADAKAAVVS
IADLASLQVEADVSESNLAKVKEGQPCEIQLDSLPDERFRGFVHMIVPTADRSKATVLVKVRFEKLDPRILPEMSAKVAF
LERPVAAGEEKPKTAVNPAAVADRDGRKVLFVLKGDRVAAVPVTLGARQGDMVEVVSGAKAGDRIVVKPLDKLKDGTRVK
TAEK

Sequences:

>Translated_404_residues
MGHEDLQKLKIDKAAALRPAARRRPLYWIGAAVALALLLVLFATGVLTPAVTVETGTVTQVYPSQTFTLLNASGYVVAQR
KAAVAAKLTGRLVWLGVEEGSRVRKGDVLARLESDDAAAGRDQAAANLGTARASLDQARAELTDASQAYERQKQLVREGI
VAKADFDAAEARFLRARAAVTAAESSIRAASAALRGAEVNLEYSLIRAPFDAVVLTKNADVGDIVTPIGAAADAKAAVVS
IADLASLQVEADVSESNLAKVKEGQPCEIQLDSLPDERFRGFVHMIVPTADRSKATVLVKVRFEKLDPRILPEMSAKVAF
LERPVAAGEEKPKTAVNPAAVADRDGRKVLFVLKGDRVAAVPVTLGARQGDMVEVVSGAKAGDRIVVKPLDKLKDGTRVK
TAEK
>Mature_403_residues
GHEDLQKLKIDKAAALRPAARRRPLYWIGAAVALALLLVLFATGVLTPAVTVETGTVTQVYPSQTFTLLNASGYVVAQRK
AAVAAKLTGRLVWLGVEEGSRVRKGDVLARLESDDAAAGRDQAAANLGTARASLDQARAELTDASQAYERQKQLVREGIV
AKADFDAAEARFLRARAAVTAAESSIRAASAALRGAEVNLEYSLIRAPFDAVVLTKNADVGDIVTPIGAAADAKAAVVSI
ADLASLQVEADVSESNLAKVKEGQPCEIQLDSLPDERFRGFVHMIVPTADRSKATVLVKVRFEKLDPRILPEMSAKVAFL
ERPVAAGEEKPKTAVNPAAVADRDGRKVLFVLKGDRVAAVPVTLGARQGDMVEVVSGAKAGDRIVVKPLDKLKDGTRVKT
AEK

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=373, Percent_Identity=28.1501340482574, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082036, Length=341, Percent_Identity=26.099706744868, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789929, Length=385, Percent_Identity=24.4155844155844, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790519, Length=305, Percent_Identity=25.5737704918033, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42841; Mature: 42709

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHEDLQKLKIDKAAALRPAARRRPLYWIGAAVALALLLVLFATGVLTPAVTVETGTVTQ
CCHHHHHHHHHHHHHHCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCEEE
VYPSQTFTLLNASGYVVAQRKAAVAAKLTGRLVWLGVEEGSRVRKGDVLARLESDDAAAG
ECCCCEEEEEECCCEEEEECHHHHHHEECCEEEEEEECCCCCCCCCCEEEEECCCCCCCC
RDQAAANLGTARASLDQARAELTDASQAYERQKQLVREGIVAKADFDAAEARFLRARAAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHH
TAAESSIRAASAALRGAEVNLEYSLIRAPFDAVVLTKNADVGDIVTPIGAAADAKAAVVS
HHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHH
IADLASLQVEADVSESNLAKVKEGQPCEIQLDSLPDERFRGFVHMIVPTADRSKATVLVK
HHHHHHEEEEECCCCCCHHHCCCCCCCEEEECCCCCHHHCCEEEEEEECCCCCCEEEEEE
VRFEKLDPRILPEMSAKVAFLERPVAAGEEKPKTAVNPAAVADRDGRKVLFVLKGDRVAA
EEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEE
VPVTLGARQGDMVEVVSGAKAGDRIVVKPLDKLKDGTRVKTAEK
EEEEECCCCCCEEEEECCCCCCCEEEECCHHHCCCCCCEECCCC
>Mature Secondary Structure 
GHEDLQKLKIDKAAALRPAARRRPLYWIGAAVALALLLVLFATGVLTPAVTVETGTVTQ
CHHHHHHHHHHHHHHCCHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCEEE
VYPSQTFTLLNASGYVVAQRKAAVAAKLTGRLVWLGVEEGSRVRKGDVLARLESDDAAAG
ECCCCEEEEEECCCEEEEECHHHHHHEECCEEEEEEECCCCCCCCCCEEEEECCCCCCCC
RDQAAANLGTARASLDQARAELTDASQAYERQKQLVREGIVAKADFDAAEARFLRARAAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHH
TAAESSIRAASAALRGAEVNLEYSLIRAPFDAVVLTKNADVGDIVTPIGAAADAKAAVVS
HHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHH
IADLASLQVEADVSESNLAKVKEGQPCEIQLDSLPDERFRGFVHMIVPTADRSKATVLVK
HHHHHHEEEEECCCCCCHHHCCCCCCCEEEECCCCCHHHCCEEEEEEECCCCCCEEEEEE
VRFEKLDPRILPEMSAKVAFLERPVAAGEEKPKTAVNPAAVADRDGRKVLFVLKGDRVAA
EEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEE
VPVTLGARQGDMVEVVSGAKAGDRIVVKPLDKLKDGTRVKTAEK
EEEEECCCCCCEEEEECCCCCCCEEEECCHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]