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The map label for this gene is cheB-2

Identifier: 39996247

GI number: 39996247

Start: 1235626

End: 1236690

Strand: Direct

Name: cheB-2

Synonym: GSU1145

Alternate gene names: 39996247

Gene position: 1235626-1236690 (Clockwise)

Preceding gene: 308513295

Following gene: 39996248

Centisome position: 32.4

GC content: 59.34

Gene sequence:

>1065_bases
ATGAAGAAAATCAAAGTACTGATTGTTGATGATTCAGCAGTGGTGCGTCAGACCATGGCGGATATTCTCGCCTCGGATCC
TCAGATCGAGGTAATGGCCACGGCTGCGGATCCCTTTATCGCAGCGGAGCGGATGAAGAGCGACGTGCCGGACGTCATTA
CCCTGGATGTGGAGATGCCGCGCATGGACGGCATCACCTTCCTCCAGAAGATAATGAGCCAGCATCCGATTCCAGTGGTC
ATGTGCTCCACGCTGACCGAGAATGGCTCTGAAACGGCCATGAAAGCCCTTGAGTACGGGGCGGTGGAGATCATCCAGAA
ACCGAAACTCGGCACAAAGCAGTTTCTCGAGGAGTCCCGGGTCAGGATCTGCGATGCTATCAAGGCTGCCAGCCAGGCGA
GGCTGAGAAAGATACCAGCCCGTCCCCACGCCGTTGCCCCAAAGCTCAGTGCCGACGTTATTCTTGAGAAGCCGGCCTCC
CGCGCCATGATCCAGACTACGGAGAGAGTTGTGGTGGTGGGGGCCTCCACTGGCGGTACCGAGGCATTGCGCGTCTTCCT
GGAGTCTTTTCCCGCCGATTGTCCGCCCATCGTCATTGTTCAGCACATGCCCGAAGGGTTCACCCGGGCCTTTGCCCAGA
GGCTCGACGGTATCTGTCGCATCTCGGTCAAGGAGGCCGCCGATAATGATTCGGTGATCCGCGGCAGAGCCCTCATCGCC
CCGGGCAACCGGCACATGCTCCTCAAGCGGAGCGGCGCCCGGTACTATGTAGAGATAAAGGACGGACCACTCGTTTCCCG
CCATCGCCCCTCGGTGGATGTTCTGTTCCGTTCGGCGGCCCGGTATGCCGGCAAGAACGCTGTGGGAGTCATTATGACCG
GCATGGGAGACGACGGCGCCAGCGGCATGCGCGAGATGAAGGATGCCGGTGCCATGACCATTGCCCAGGACGAAGCGAGC
TGCGTGGTGTTCGGCATGCCCAACGAAGCCATCAAACGCGGTGGTACGGTCAAGGTGCTGCCTTTGGAATCCATTGCTGC
GGACGTTATCCGGCACTGTAGTTGA

Upstream 100 bases:

>100_bases
AAGCTCTTCTTCTATACCCATACGGGTGAGGTGCTGCTCAAACGGTTGAACCGCACCGAGGCGGACAGTTGAGCCGAAAA
AGGCCGGGGTGGGCGGATTC

Downstream 100 bases:

>100_bases
ATTGGGAGTAACGAGCAGATAGTGTGAGCGCGTTTCCTGTCGGAGAGCTACGCATTTCTGGACAAAGCCGGTCGGTAGAT
TATTATGTGATAATGATTCC

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKKIKVLIVDDSAVVRQTMADILASDPQIEVMATAADPFIAAERMKSDVPDVITLDVEMPRMDGITFLQKIMSQHPIPVV
MCSTLTENGSETAMKALEYGAVEIIQKPKLGTKQFLEESRVRICDAIKAASQARLRKIPARPHAVAPKLSADVILEKPAS
RAMIQTTERVVVVGASTGGTEALRVFLESFPADCPPIVIVQHMPEGFTRAFAQRLDGICRISVKEAADNDSVIRGRALIA
PGNRHMLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGASGMREMKDAGAMTIAQDEAS
CVVFGMPNEAIKRGGTVKVLPLESIAADVIRHCS

Sequences:

>Translated_354_residues
MKKIKVLIVDDSAVVRQTMADILASDPQIEVMATAADPFIAAERMKSDVPDVITLDVEMPRMDGITFLQKIMSQHPIPVV
MCSTLTENGSETAMKALEYGAVEIIQKPKLGTKQFLEESRVRICDAIKAASQARLRKIPARPHAVAPKLSADVILEKPAS
RAMIQTTERVVVVGASTGGTEALRVFLESFPADCPPIVIVQHMPEGFTRAFAQRLDGICRISVKEAADNDSVIRGRALIA
PGNRHMLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGASGMREMKDAGAMTIAQDEAS
CVVFGMPNEAIKRGGTVKVLPLESIAADVIRHCS
>Mature_354_residues
MKKIKVLIVDDSAVVRQTMADILASDPQIEVMATAADPFIAAERMKSDVPDVITLDVEMPRMDGITFLQKIMSQHPIPVV
MCSTLTENGSETAMKALEYGAVEIIQKPKLGTKQFLEESRVRICDAIKAASQARLRKIPARPHAVAPKLSADVILEKPAS
RAMIQTTERVVVVGASTGGTEALRVFLESFPADCPPIVIVQHMPEGFTRAFAQRLDGICRISVKEAADNDSVIRGRALIA
PGNRHMLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGASGMREMKDAGAMTIAQDEAS
CVVFGMPNEAIKRGGTVKVLPLESIAADVIRHCS

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=354, Percent_Identity=50.2824858757062, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_GEOSL (P62639)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952198.1
- ProteinModelPortal:   P62639
- SMR:   P62639
- GeneID:   2685549
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1145
- NMPDR:   fig|243231.1.peg.1136
- TIGR:   GSU1145
- HOGENOM:   HBG705324
- OMA:   PGGKHML
- ProtClustDB:   CLSK828211
- BioCyc:   GSUL243231:GSU_1145-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38277; Mature: 38277

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 176-176 ACT_SITE 202-202 ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKVLIVDDSAVVRQTMADILASDPQIEVMATAADPFIAAERMKSDVPDVITLDVEMP
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECC
RMDGITFLQKIMSQHPIPVVMCSTLTENGSETAMKALEYGAVEIIQKPKLGTKQFLEESR
CCCCHHHHHHHHHCCCCCEEEEEHHCCCCHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
VRICDAIKAASQARLRKIPARPHAVAPKLSADVILEKPASRAMIQTTERVVVVGASTGGT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHEECCEEEEEECCCCCH
EALRVFLESFPADCPPIVIVQHMPEGFTRAFAQRLDGICRISVKEAADNDSVIRGRALIA
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEECEEEEC
PGNRHMLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGA
CCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCH
SGMREMKDAGAMTIAQDEASCVVFGMPNEAIKRGGTVKVLPLESIAADVIRHCS
HHHHHHHHCCCEEEEECCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIKVLIVDDSAVVRQTMADILASDPQIEVMATAADPFIAAERMKSDVPDVITLDVEMP
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECC
RMDGITFLQKIMSQHPIPVVMCSTLTENGSETAMKALEYGAVEIIQKPKLGTKQFLEESR
CCCCHHHHHHHHHCCCCCEEEEEHHCCCCHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHH
VRICDAIKAASQARLRKIPARPHAVAPKLSADVILEKPASRAMIQTTERVVVVGASTGGT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHEECCEEEEEECCCCCH
EALRVFLESFPADCPPIVIVQHMPEGFTRAFAQRLDGICRISVKEAADNDSVIRGRALIA
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEECEEEEC
PGNRHMLLKRSGARYYVEIKDGPLVSRHRPSVDVLFRSAARYAGKNAVGVIMTGMGDDGA
CCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCH
SGMREMKDAGAMTIAQDEASCVVFGMPNEAIKRGGTVKVLPLESIAADVIRHCS
HHHHHHHHCCCEEEEECCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA