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The map label for this gene is tyrS

Identifier: 39996241

GI number: 39996241

Start: 1222614

End: 1223825

Strand: Direct

Name: tyrS

Synonym: GSU1139

Alternate gene names: 39996241

Gene position: 1222614-1223825 (Clockwise)

Preceding gene: 39996240

Following gene: 39996242

Centisome position: 32.05

GC content: 55.69

Gene sequence:

>1212_bases
ATGTCCGTTGCCGATCAAATGGCGCTCATCAAGCGGGGCGCGGTAGAGATACTGGTAGAAAAGGAACTAGAGGAAAAACT
CGAAAAGTCGGCCAAGACCGGGGTTCCTCTCAAGATAAAGGCCGGCTTCGATCCCACTGCGCCCGATCTTCACTTAGGAC
ACACGGTCCTTTTGCACAAGATGCGTCAGTTCCAGCAGCTTGGGCACGAGGTTATTTTTCTGATCGGTGATTTTACCGGC
ATGATTGGTGATCCGACCGGCAAGTCCGAAACGCGCAAGGCACTCAGCCGAGAGGATGTTCTGCGCAACGCCGAAACTTA
CAAGGAGCAGGTGTTTAAAATCCTCGATCCGGAGAAAACACGGGTGGCCTTCAACTCGGAGTGGCTGGCCAAGCTCGACG
CCGGCGGGATGATTGGCCTTGCCGCCAAGTACACCGTGGCGCGGATGCTTGAGCGCGACGACTTCGGGAAAAGGTTTGCG
AACCAGCTTCCCATAAGCATTCACGAGTTTCTCTATCCCTTGATCCAGGGATATGATTCGGTGGCGCTTCAGGCAGATGT
GGAACTCGGAGGAACCGATCAGAAGTTCAACCTACTTGTGGGGCGCGAACTTCAGCGTGAGTGGGGGCAAACCCCTCAGA
CCGTAATCACGATGCCGCTGCTTGAAGGGCTCGACGGCGTCAACAAGATGAGCAAATCTCTCGGCAACTACATTGGAATC
AACGAACCGGCCGACGAAATATTCGGCAAGATCATGTCGATATCCGATGAGCTCATGCTTCGCTACTATGAATTGCTGAG
TGATCTCTCCATGGCCGAAATCGACGGCATGCGAACCGGTATCCGCGATGGTTCGGTTCATCCTATGGAAGCCAAGAAGC
AGCTTGGTCGGGAGGTCGTTGCCCGTTACCACGGTGCTGCAGCGGCCACTGATGCCGAGGAGCATTTCGTCAAGCGGTTC
AGGGATAACCAGACGCCCGACGAAATGCCCGAACTGACCCTTGCGGCGACTGATGAAAAGGTTGCGCTCTGCCGGCTCCT
GGCAGAAGCGGGGCTCGTGAAGTCCAACAGTGAAGGCCGGCGTGCCATCCAGCAGGGTGGTGTCAAAGTCAACGGCGAAA
AGGTGTCGGATGAGAGTCTGGAACTTGCCGCCACGGGGGTGTATGTCATTCAGTTCGGCAAGCGTCGTTTCGCCCGCATC
ACCTTTGCATGA

Upstream 100 bases:

>100_bases
GACTTAACGGAGTTGTCATTGGTGTCGATCCGGCCAGTGGCCGGGCACTCTCCATTGAGCGCATTTCACTAATCTGTTCG
TGAGGAGGATTGCCCAGGCC

Downstream 100 bases:

>100_bases
AGTAAAGTTACGACGAATCAAGTTGTTAACTCGCTGAAAACACACTAATAATTTTTTTGAAAAAAATCCTTGACCGGTGG
GGAATGATTTGGTAGTTTCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSVADQMALIKRGAVEILVEKELEEKLEKSAKTGVPLKIKAGFDPTAPDLHLGHTVLLHKMRQFQQLGHEVIFLIGDFTG
MIGDPTGKSETRKALSREDVLRNAETYKEQVFKILDPEKTRVAFNSEWLAKLDAGGMIGLAAKYTVARMLERDDFGKRFA
NQLPISIHEFLYPLIQGYDSVALQADVELGGTDQKFNLLVGRELQREWGQTPQTVITMPLLEGLDGVNKMSKSLGNYIGI
NEPADEIFGKIMSISDELMLRYYELLSDLSMAEIDGMRTGIRDGSVHPMEAKKQLGREVVARYHGAAAATDAEEHFVKRF
RDNQTPDEMPELTLAATDEKVALCRLLAEAGLVKSNSEGRRAIQQGGVKVNGEKVSDESLELAATGVYVIQFGKRRFARI
TFA

Sequences:

>Translated_403_residues
MSVADQMALIKRGAVEILVEKELEEKLEKSAKTGVPLKIKAGFDPTAPDLHLGHTVLLHKMRQFQQLGHEVIFLIGDFTG
MIGDPTGKSETRKALSREDVLRNAETYKEQVFKILDPEKTRVAFNSEWLAKLDAGGMIGLAAKYTVARMLERDDFGKRFA
NQLPISIHEFLYPLIQGYDSVALQADVELGGTDQKFNLLVGRELQREWGQTPQTVITMPLLEGLDGVNKMSKSLGNYIGI
NEPADEIFGKIMSISDELMLRYYELLSDLSMAEIDGMRTGIRDGSVHPMEAKKQLGREVVARYHGAAAATDAEEHFVKRF
RDNQTPDEMPELTLAATDEKVALCRLLAEAGLVKSNSEGRRAIQQGGVKVNGEKVSDESLELAATGVYVIQFGKRRFARI
TFA
>Mature_402_residues
SVADQMALIKRGAVEILVEKELEEKLEKSAKTGVPLKIKAGFDPTAPDLHLGHTVLLHKMRQFQQLGHEVIFLIGDFTGM
IGDPTGKSETRKALSREDVLRNAETYKEQVFKILDPEKTRVAFNSEWLAKLDAGGMIGLAAKYTVARMLERDDFGKRFAN
QLPISIHEFLYPLIQGYDSVALQADVELGGTDQKFNLLVGRELQREWGQTPQTVITMPLLEGLDGVNKMSKSLGNYIGIN
EPADEIFGKIMSISDELMLRYYELLSDLSMAEIDGMRTGIRDGSVHPMEAKKQLGREVVARYHGAAAATDAEEHFVKRFR
DNQTPDEMPELTLAATDEKVALCRLLAEAGLVKSNSEGRRAIQQGGVKVNGEKVSDESLELAATGVYVIQFGKRRFARIT
FA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=433, Percent_Identity=27.4826789838337, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1787925, Length=415, Percent_Identity=27.4698795180723, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=399, Percent_Identity=26.8170426065163, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=25.7403189066059, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI20130383, Length=406, Percent_Identity=24.8768472906404, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_GEOSL (Q74E25)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952192.1
- ProteinModelPortal:   Q74E25
- SMR:   Q74E25
- GeneID:   2685312
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1139
- NMPDR:   fig|243231.1.peg.1130
- TIGR:   GSU1139
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   GSUL243231:GSU_1139-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44772; Mature: 44641

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVADQMALIKRGAVEILVEKELEEKLEKSAKTGVPLKIKAGFDPTAPDLHLGHTVLLHK
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHH
MRQFQQLGHEVIFLIGDFTGMIGDPTGKSETRKALSREDVLRNAETYKEQVFKILDPEKT
HHHHHHCCHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RVAFNSEWLAKLDAGGMIGLAAKYTVARMLERDDFGKRFANQLPISIHEFLYPLIQGYDS
EEEECCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
VALQADVELGGTDQKFNLLVGRELQREWGQTPQTVITMPLLEGLDGVNKMSKSLGNYIGI
EEEEEECEECCCCCHHHHHHHHHHHHHHCCCCHHEEHHHHHHCCCHHHHHHHHHCCCCCC
NEPADEIFGKIMSISDELMLRYYELLSDLSMAEIDGMRTGIRDGSVHPMEAKKQLGREVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ARYHGAAAATDAEEHFVKRFRDNQTPDEMPELTLAATDEKVALCRLLAEAGLVKSNSEGR
HHHCCCHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHH
RAIQQGGVKVNGEKVSDESLELAATGVYVIQFGKRRFARITFA
HHHHHCCEEECCCCCCCCCHHHHHHHEEEEEECCHHHEEEECC
>Mature Secondary Structure 
SVADQMALIKRGAVEILVEKELEEKLEKSAKTGVPLKIKAGFDPTAPDLHLGHTVLLHK
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHH
MRQFQQLGHEVIFLIGDFTGMIGDPTGKSETRKALSREDVLRNAETYKEQVFKILDPEKT
HHHHHHCCHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RVAFNSEWLAKLDAGGMIGLAAKYTVARMLERDDFGKRFANQLPISIHEFLYPLIQGYDS
EEEECCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
VALQADVELGGTDQKFNLLVGRELQREWGQTPQTVITMPLLEGLDGVNKMSKSLGNYIGI
EEEEEECEECCCCCHHHHHHHHHHHHHHCCCCHHEEHHHHHHCCCHHHHHHHHHCCCCCC
NEPADEIFGKIMSISDELMLRYYELLSDLSMAEIDGMRTGIRDGSVHPMEAKKQLGREVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ARYHGAAAATDAEEHFVKRFRDNQTPDEMPELTLAATDEKVALCRLLAEAGLVKSNSEGR
HHHCCCHHCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHH
RAIQQGGVKVNGEKVSDESLELAATGVYVIQFGKRRFARITFA
HHHHHCCEEECCCCCCCCCHHHHHHHEEEEEECCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA