Click here to switch to the map view.

The map label for this gene is kinA [H]

Identifier: 39996221

GI number: 39996221

Start: 1203159

End: 1204421

Strand: Direct

Name: kinA [H]

Synonym: GSU1119

Alternate gene names: 39996221

Gene position: 1203159-1204421 (Clockwise)

Preceding gene: 39996220

Following gene: 39996222

Centisome position: 31.54

GC content: 57.64

Gene sequence:

>1263_bases
TTGCCCGCCGGTCCGTCAGCTGCTATGACTGCACGACCCGCAGTCCACTCACCCCTGAAAGTACGCCATACAGCCATGAT
GACGGAAAAGAATGAATCCAAGGGTACCATCCTTATTATCGATGACGAAAAAGTCATTCTCGACCTGACCTCCATCGTTC
TCAGAAATCGCGGCTACACGGTCCATACCGCGTCAGACGCCACATCCGGCATGGCTGTGCTGGAAGAGTGCCGGCCGCAG
ATGGTGCTCCTCGACTACATGATGCCGATGGTGGATGGTCTCACCGCGCTCAAGCAGATCCGGCAGCGTTACCCCGACAC
GTATGTGATCATCTTCACCGGCAAGGGAAACGAGGAACTGGCCGTAGAGCTGATGAAGGCAGGGGCCTCAGACTACATCG
TCAAACCGTTCAATAACCAGAATCTGGTCGAGCGGATCGAGAATGTGCTCCGGATCAGGGATGTCGAGCTCAGAAACCAG
GAGCTGCTCCAGGAGCGTGAGCTGCTTCTGGCGGAGATCGAGGCATGGAACCAGGAGCTCGAACGCCGCGTCCAGCAGAA
AAGCGAGGCCCTTCAGCAGGCCCAGGCCGAGATCGTGCAGTCCGAAAAACTCGCATCTCTCGGCTATCTCTCCGCGGGCA
TGGCCCATGAAATCCGCAACCCCCTCAACTCCATTTCACTGTTCGCCCAACTGCTCAAGAGCACGCTTGATGATCCGGAA
AAAGTGGGCTATGTCGACAAGATTCTCAAGGAAGCCGACCGGATAGACGACATCATGCGCAAGCTTCTGGATGCCTCGAA
GCGGCCCCGCTTCCAGTTGAGTGAGGTCCGGCTCGATCGGGTCATTGAGGCGACCCTCGAGGCGTTTCGTCCCCAGATAT
CGCTCCATGGCATCGCGGTGGTGAAAGAGTTCCGTCGGATCCCTCCTCCATTCCAGGCTGACCCGGCCGAGATCGAGCAG
ATTTTTACCAATCTCTTTCTCAACTCCATCCACGAGATGAACGACGGGGGATCCCTAACGGTGGTGCTCGACCAGGACGA
GCGAAACATGGTCATCAGAATTGCCGACACGGGCCCCGGTATCCCCCGGGAGCACGTGGCGAAGATATTCGACCCGTTCT
TCACCACCAAGACGAGCGGCAGCGGGCTCGGCCTTGCTGTGGTGCTCAGGATCGTCAAGACCTATGACGGGCGGATCGAT
GTTGAGAAGAGCGATGAGAGCGGCACTGTCTTCGCTATCCGGCTTCCTCTTTCCGGAGTGTGA

Upstream 100 bases:

>100_bases
CCAAGTGCCCGGTCATGACCGTGCGCCCGCCCGAAGTCTGAGACATATCCCTTACAGGGCAACAGACAGGGGCAGCCACC
GCGCTGCCCCTCGTTTTTTC

Downstream 100 bases:

>100_bases
ACCGGCCCTTTGCCCTTTTCTTTTCACGGGTGATTTTGTATGATTGCCCACACTTTCAACGCACGACGGAGGGCGTATGG
ACGCCGGCCGGAGAACGATC

Product: sensor histidine kinase/response regulator

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPAGPSAAMTARPAVHSPLKVRHTAMMTEKNESKGTILIIDDEKVILDLTSIVLRNRGYTVHTASDATSGMAVLEECRPQ
MVLLDYMMPMVDGLTALKQIRQRYPDTYVIIFTGKGNEELAVELMKAGASDYIVKPFNNQNLVERIENVLRIRDVELRNQ
ELLQERELLLAEIEAWNQELERRVQQKSEALQQAQAEIVQSEKLASLGYLSAGMAHEIRNPLNSISLFAQLLKSTLDDPE
KVGYVDKILKEADRIDDIMRKLLDASKRPRFQLSEVRLDRVIEATLEAFRPQISLHGIAVVKEFRRIPPPFQADPAEIEQ
IFTNLFLNSIHEMNDGGSLTVVLDQDERNMVIRIADTGPGIPREHVAKIFDPFFTTKTSGSGLGLAVVLRIVKTYDGRID
VEKSDESGTVFAIRLPLSGV

Sequences:

>Translated_420_residues
MPAGPSAAMTARPAVHSPLKVRHTAMMTEKNESKGTILIIDDEKVILDLTSIVLRNRGYTVHTASDATSGMAVLEECRPQ
MVLLDYMMPMVDGLTALKQIRQRYPDTYVIIFTGKGNEELAVELMKAGASDYIVKPFNNQNLVERIENVLRIRDVELRNQ
ELLQERELLLAEIEAWNQELERRVQQKSEALQQAQAEIVQSEKLASLGYLSAGMAHEIRNPLNSISLFAQLLKSTLDDPE
KVGYVDKILKEADRIDDIMRKLLDASKRPRFQLSEVRLDRVIEATLEAFRPQISLHGIAVVKEFRRIPPPFQADPAEIEQ
IFTNLFLNSIHEMNDGGSLTVVLDQDERNMVIRIADTGPGIPREHVAKIFDPFFTTKTSGSGLGLAVVLRIVKTYDGRID
VEKSDESGTVFAIRLPLSGV
>Mature_419_residues
PAGPSAAMTARPAVHSPLKVRHTAMMTEKNESKGTILIIDDEKVILDLTSIVLRNRGYTVHTASDATSGMAVLEECRPQM
VLLDYMMPMVDGLTALKQIRQRYPDTYVIIFTGKGNEELAVELMKAGASDYIVKPFNNQNLVERIENVLRIRDVELRNQE
LLQERELLLAEIEAWNQELERRVQQKSEALQQAQAEIVQSEKLASLGYLSAGMAHEIRNPLNSISLFAQLLKSTLDDPEK
VGYVDKILKEADRIDDIMRKLLDASKRPRFQLSEVRLDRVIEATLEAFRPQISLHGIAVVKEFRRIPPPFQADPAEIEQI
FTNLFLNSIHEMNDGGSLTVVLDQDERNMVIRIADTGPGIPREHVAKIFDPFFTTKTSGSGLGLAVVLRIVKTYDGRIDV
EKSDESGTVFAIRLPLSGV

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=230, Percent_Identity=34.3478260869565, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=34.2105263157895, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=27.8884462151394, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=29.4573643410853, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=298, Percent_Identity=28.5234899328859, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=36.2745098039216, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=215, Percent_Identity=27.4418604651163, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=35.8974358974359, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790346, Length=217, Percent_Identity=25.8064516129032, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=37.6068376068376, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994928, Length=216, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=264, Percent_Identity=25.3787878787879, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=26.6968325791855, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788394, Length=121, Percent_Identity=32.2314049586777, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=22.2727272727273, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=33.0578512396694, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=31.5789473684211, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=101, Percent_Identity=34.6534653465347, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47113; Mature: 46982

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGPSAAMTARPAVHSPLKVRHTAMMTEKNESKGTILIIDDEKVILDLTSIVLRNRGYT
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCE
VHTASDATSGMAVLEECRPQMVLLDYMMPMVDGLTALKQIRQRYPDTYVIIFTGKGNEEL
EEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHH
AVELMKAGASDYIVKPFNNQNLVERIENVLRIRDVELRNQELLQERELLLAEIEAWNQEL
HHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRVQQKSEALQQAQAEIVQSEKLASLGYLSAGMAHEIRNPLNSISLFAQLLKSTLDDPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KVGYVDKILKEADRIDDIMRKLLDASKRPRFQLSEVRLDRVIEATLEAFRPQISLHGIAV
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VKEFRRIPPPFQADPAEIEQIFTNLFLNSIHEMNDGGSLTVVLDQDERNMVIRIADTGPG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCC
IPREHVAKIFDPFFTTKTSGSGLGLAVVLRIVKTYDGRIDVEKSDESGTVFAIRLPLSGV
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCC
>Mature Secondary Structure 
PAGPSAAMTARPAVHSPLKVRHTAMMTEKNESKGTILIIDDEKVILDLTSIVLRNRGYT
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCE
VHTASDATSGMAVLEECRPQMVLLDYMMPMVDGLTALKQIRQRYPDTYVIIFTGKGNEEL
EEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHH
AVELMKAGASDYIVKPFNNQNLVERIENVLRIRDVELRNQELLQERELLLAEIEAWNQEL
HHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRVQQKSEALQQAQAEIVQSEKLASLGYLSAGMAHEIRNPLNSISLFAQLLKSTLDDPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KVGYVDKILKEADRIDDIMRKLLDASKRPRFQLSEVRLDRVIEATLEAFRPQISLHGIAV
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VKEFRRIPPPFQADPAEIEQIFTNLFLNSIHEMNDGGSLTVVLDQDERNMVIRIADTGPG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCC
IPREHVAKIFDPFFTTKTSGSGLGLAVVLRIVKTYDGRIDVEKSDESGTVFAIRLPLSGV
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]