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The map label for this gene is pstA [H]

Identifier: 39996199

GI number: 39996199

Start: 1182031

End: 1182951

Strand: Reverse

Name: pstA [H]

Synonym: GSU1097

Alternate gene names: 39996199

Gene position: 1182951-1182031 (Counterclockwise)

Preceding gene: 39996200

Following gene: 39996198

Centisome position: 31.01

GC content: 63.08

Gene sequence:

>921_bases
ATGCAACAGTCCGTTACGGAAATCAGGCGGCTCATCGCCCGCCACAAGTTCTGGGATTACGTGTTCGCCATCGTGGGTCT
CATCTGCCTGCTGGTGGGGATTCTCACCCTCATGGGGCTGTTCGCCCAACTGGTGGCGGACGGCGCGCCGCGGCTGAAGC
CCCACTTCTTCACCTCCTTCCCCTCGCGCTTCCCTGAGGAGGCGGGGATTCTCTCGGCCTGGGTCGGCACCATCCTGGTC
ATGCTGGTGACCGCCTTCGTGGCGGTGCCGCTGGGAGTCGCCGCCGGCGTCTACCTGGAAGAGTACGCTCCCAAGAACTG
GCTCACGGCCATTATCGAGATCAACGTGACGAACCTGGCCGGGGTCCCCTCCATCGTCTTCGGCCTGCTGGCTCTCGGTC
TCTTCGTCTACCAGTTGAACTTCGGCCAGAGCATCCTGTCCGCCGGCCTCACCCTGGCGCTGCTGATCCTGCCGATCGTG
ATCGTGGCCACCCGGGAATCGCTACGCGCCGTGCCCGGCAGCATCCGCGAGGCGGCCTATGCCCTGGGCGCCACCCGCTG
GCAGATGGTTTCCAATCACGTGCTCCCCTACTCTTCATCCGGCATTCTCACGGGGGTAATTATCGGCCTGTCGCGGGCCA
TCGGCGAGACGGCGCCGGTCATCACCATCGGGGCACTAACCTTCATCGCGTTTCTGCCCCATTCGCCGATTTCCGGCCAG
TTTCCCTTCATCAGCTTCGAATGGCTCAAGGATCCGTTCACGGTCATGCCGATCCAGATGTTCAACTGGACCTCCCGGCC
GGAGGAGGAGTTCCAGCTCAACGCGGCGGCAGCCGGCGTGGTGCTCCTGGCCATGACCCTGGCCATGAACGCGGTGGCGA
TCTTTCTCCGCTACCATATCCGTAAAAAGATCAAGTGGTGA

Upstream 100 bases:

>100_bases
ACCAGTCGATCTTCGCCGCCGGACTCACCCTGATGGTGATGACCCTGGTGTTCAACATCATTGGCTACTGGCTCAAAAAG
CGGTTCCGGGAGGTCTACTG

Downstream 100 bases:

>100_bases
ACGGCCGGGACCGTTCAGAGCGCCTTTACCGTACTATAGATACCCAAGGAGCAACGATGTCAGCATCTGTCCAGCCTGTA
CTCAAGGCCGAATCGAAAAA

Product: phosphate ABC transporter periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQQSVTEIRRLIARHKFWDYVFAIVGLICLLVGILTLMGLFAQLVADGAPRLKPHFFTSFPSRFPEEAGILSAWVGTILV
MLVTAFVAVPLGVAAGVYLEEYAPKNWLTAIIEINVTNLAGVPSIVFGLLALGLFVYQLNFGQSILSAGLTLALLILPIV
IVATRESLRAVPGSIREAAYALGATRWQMVSNHVLPYSSSGILTGVIIGLSRAIGETAPVITIGALTFIAFLPHSPISGQ
FPFISFEWLKDPFTVMPIQMFNWTSRPEEEFQLNAAAAGVVLLAMTLAMNAVAIFLRYHIRKKIKW

Sequences:

>Translated_306_residues
MQQSVTEIRRLIARHKFWDYVFAIVGLICLLVGILTLMGLFAQLVADGAPRLKPHFFTSFPSRFPEEAGILSAWVGTILV
MLVTAFVAVPLGVAAGVYLEEYAPKNWLTAIIEINVTNLAGVPSIVFGLLALGLFVYQLNFGQSILSAGLTLALLILPIV
IVATRESLRAVPGSIREAAYALGATRWQMVSNHVLPYSSSGILTGVIIGLSRAIGETAPVITIGALTFIAFLPHSPISGQ
FPFISFEWLKDPFTVMPIQMFNWTSRPEEEFQLNAAAAGVVLLAMTLAMNAVAIFLRYHIRKKIKW
>Mature_306_residues
MQQSVTEIRRLIARHKFWDYVFAIVGLICLLVGILTLMGLFAQLVADGAPRLKPHFFTSFPSRFPEEAGILSAWVGTILV
MLVTAFVAVPLGVAAGVYLEEYAPKNWLTAIIEINVTNLAGVPSIVFGLLALGLFVYQLNFGQSILSAGLTLALLILPIV
IVATRESLRAVPGSIREAAYALGATRWQMVSNHVLPYSSSGILTGVIIGLSRAIGETAPVITIGALTFIAFLPHSPISGQ
FPFISFEWLKDPFTVMPIQMFNWTSRPEEEFQLNAAAAGVVLLAMTLAMNAVAIFLRYHIRKKIKW

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=293, Percent_Identity=31.740614334471, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790164, Length=231, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33428; Mature: 33428

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQSVTEIRRLIARHKFWDYVFAIVGLICLLVGILTLMGLFAQLVADGAPRLKPHFFTSF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
PSRFPEEAGILSAWVGTILVMLVTAFVAVPLGVAAGVYLEEYAPKNWLTAIIEINVTNLA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHC
GVPSIVFGLLALGLFVYQLNFGQSILSAGLTLALLILPIVIVATRESLRAVPGSIREAAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ALGATRWQMVSNHVLPYSSSGILTGVIIGLSRAIGETAPVITIGALTFIAFLPHSPISGQ
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
FPFISFEWLKDPFTVMPIQMFNWTSRPEEEFQLNAAAAGVVLLAMTLAMNAVAIFLRYHI
CCCEEHHHHCCCCCEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKIKW
HHHCCC
>Mature Secondary Structure
MQQSVTEIRRLIARHKFWDYVFAIVGLICLLVGILTLMGLFAQLVADGAPRLKPHFFTSF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
PSRFPEEAGILSAWVGTILVMLVTAFVAVPLGVAAGVYLEEYAPKNWLTAIIEINVTNLA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECHHHC
GVPSIVFGLLALGLFVYQLNFGQSILSAGLTLALLILPIVIVATRESLRAVPGSIREAAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ALGATRWQMVSNHVLPYSSSGILTGVIIGLSRAIGETAPVITIGALTFIAFLPHSPISGQ
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
FPFISFEWLKDPFTVMPIQMFNWTSRPEEEFQLNAAAAGVVLLAMTLAMNAVAIFLRYHI
CCCEEHHHHCCCCCEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKKIKW
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]