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The map label for this gene is pilJ [H]

Identifier: 39996132

GI number: 39996132

Start: 1107913

End: 1109562

Strand: Direct

Name: pilJ [H]

Synonym: GSU1029

Alternate gene names: 39996132

Gene position: 1107913-1109562 (Clockwise)

Preceding gene: 39996126

Following gene: 39996133

Centisome position: 29.05

GC content: 61.82

Gene sequence:

>1650_bases
ATGAGCGCATGGCGGGACTTGAAGGTGAGAACAAAAATTTTCGTCCTGGTGATTGCCGGATGTCTGGGGCTCGTGGTACT
GGGATCGGTGGCGCTTTACAATATGCGCAACCTGAGCGGCAGCGTGAAGGAAGCCAACATCGGCATGGAGCACGTGGCGG
GGCTTTCCGGTATGAAGAGCGACTTTCTCGAGATGAGGCTGGCGCTCGTCTACATGCTTGCCCTGAAAGATGCGGAAAAG
ATCGGCGGCAAGGAACAGGATTTCCTGAAGGCCGCTGACAGGATCAAAAAGACACTCGACGACCTGGGCAAGCAGGAACT
GACTGACACCGAGAAAAAGTCCCTCGTCGAGTTCAGGGGTGGCTTCGAGTCTTATGTCGAGAAGGGAACGAGACTCGCCG
AGCTAATCAAGGACGCTACCGCCAAGGGAGACGAGGTGGGCCGGGCCGATGCCATGACCTTCGCCACCCAGAGCGTGGCC
CCCCTCTACGATACCCCGGCCAAGATCATTGCCTCGATGGTGCAGGAAAATATCGGCGAAGCTCATAAGATGTATGAGCA
GGACATGGCCTCGTACCGAGCTTCCTTCATTATGATGGTGGTGATCATTCTGGGGGTGATCGGCGTCGCCGCTGCGGCGG
GCCTGGCCATCGCCGGTTCCATCAGCGGTCCTCTCAACAAGGTGCTCGATGTACTCACCCGCGTGGCCGCCGGTGACCTG
ACAGCCCGGGCCGACGTCGTCAGTGCCGACGAAATGGGGCTGCTGGCGCGTGAGGTGAACACTACCGCGGCCAAGATCAA
CGAGATCATCGGCCTTGTTGCCCACAATGCCTCCCAGGTGACTGCCGCGGCGACCCAGCTCCATGCCACCTCCACCCAGA
TGTCCACCGGCGCTGAGGAGGTGGCCCAACAGGCCGCCACCGTGGCCACGGCCAGTGAGGAGATGGCTGCCACCTCGGCC
GAGATCGCCCATAACTGCTCCCTGGCGGCTGAAAGCTCCCGACACGCCAACGATCGGGCCGAGAACGGTTCGGATGTGGT
GCAGGAAACCCTGACCGTCATGAACCGCATCGCCGAGCGGGTGAAGGATTCGGCACGCACCGTCGAATCTCTGGGCGAGC
GGAGCGACCAGATCGGCGAGATCATCGGCACTATCCAGGACATCGCCGACCAGACCAATCTCCTTGCTCTCAACGCGGCC
ATCGAAGCCGCCCGCGCTGGCGAACAGGGACGCGGCTTCGCCGTGGTCGCCGACGAGGTTCGGGCCCTGGCCGAGCGGAC
AACCAAGGCCACCAAGGAGATATCCCAGATGATCAAGGCGATCCAGGGGGAAACCAAGGGCGCCGTCACTTCCATGGAGG
AGGGGGTCAAAGAGGTGGAAAAGGGAACCTCGGACGCATCCAAATCGGGCGAGGCCCTGCAGGCGATCCTGGAGCAGATC
GGCGGCGTTACCATGCAGGTGAGCCAGATTGCCACTGCCGCCGAGGAACAGACCGCGACCACGGGTGAGATCAACAACAA
CATCCAGCAGATCACGGAGGTGGTCCAGCTCACCGCGCGGGGCGCCGAAGAGTCGGCCCAGGCCGCCGAGCAGCTGGCGA
AACTGGCCGAGGAACTGCAGGACCTGGTGTACAAGTTCAAACTCGCCTGA

Upstream 100 bases:

>100_bases
AGTTTAGTCGAACCGTGCCGATAGGGTTACGTAAACAGTCATGGCATTAGTTGGTAATGCCGAATCGGATAATTACTTGT
CTGGAAGGAGATGGGATACG

Downstream 100 bases:

>100_bases
TCAGCCATCATATCCGTAAAAAAACCCGGCAACCGCCGGGTTTTTTTTGTGCTCCGCTCCCGGGTCCGCCATGGACGGCA
ACTGCCGGGATCATATCAGG

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSAWRDLKVRTKIFVLVIAGCLGLVVLGSVALYNMRNLSGSVKEANIGMEHVAGLSGMKSDFLEMRLALVYMLALKDAEK
IGGKEQDFLKAADRIKKTLDDLGKQELTDTEKKSLVEFRGGFESYVEKGTRLAELIKDATAKGDEVGRADAMTFATQSVA
PLYDTPAKIIASMVQENIGEAHKMYEQDMASYRASFIMMVVIILGVIGVAAAAGLAIAGSISGPLNKVLDVLTRVAAGDL
TARADVVSADEMGLLAREVNTTAAKINEIIGLVAHNASQVTAAATQLHATSTQMSTGAEEVAQQAATVATASEEMAATSA
EIAHNCSLAAESSRHANDRAENGSDVVQETLTVMNRIAERVKDSARTVESLGERSDQIGEIIGTIQDIADQTNLLALNAA
IEAARAGEQGRGFAVVADEVRALAERTTKATKEISQMIKAIQGETKGAVTSMEEGVKEVEKGTSDASKSGEALQAILEQI
GGVTMQVSQIATAAEEQTATTGEINNNIQQITEVVQLTARGAEESAQAAEQLAKLAEELQDLVYKFKLA

Sequences:

>Translated_549_residues
MSAWRDLKVRTKIFVLVIAGCLGLVVLGSVALYNMRNLSGSVKEANIGMEHVAGLSGMKSDFLEMRLALVYMLALKDAEK
IGGKEQDFLKAADRIKKTLDDLGKQELTDTEKKSLVEFRGGFESYVEKGTRLAELIKDATAKGDEVGRADAMTFATQSVA
PLYDTPAKIIASMVQENIGEAHKMYEQDMASYRASFIMMVVIILGVIGVAAAAGLAIAGSISGPLNKVLDVLTRVAAGDL
TARADVVSADEMGLLAREVNTTAAKINEIIGLVAHNASQVTAAATQLHATSTQMSTGAEEVAQQAATVATASEEMAATSA
EIAHNCSLAAESSRHANDRAENGSDVVQETLTVMNRIAERVKDSARTVESLGERSDQIGEIIGTIQDIADQTNLLALNAA
IEAARAGEQGRGFAVVADEVRALAERTTKATKEISQMIKAIQGETKGAVTSMEEGVKEVEKGTSDASKSGEALQAILEQI
GGVTMQVSQIATAAEEQTATTGEINNNIQQITEVVQLTARGAEESAQAAEQLAKLAEELQDLVYKFKLA
>Mature_548_residues
SAWRDLKVRTKIFVLVIAGCLGLVVLGSVALYNMRNLSGSVKEANIGMEHVAGLSGMKSDFLEMRLALVYMLALKDAEKI
GGKEQDFLKAADRIKKTLDDLGKQELTDTEKKSLVEFRGGFESYVEKGTRLAELIKDATAKGDEVGRADAMTFATQSVAP
LYDTPAKIIASMVQENIGEAHKMYEQDMASYRASFIMMVVIILGVIGVAAAAGLAIAGSISGPLNKVLDVLTRVAAGDLT
ARADVVSADEMGLLAREVNTTAAKINEIIGLVAHNASQVTAAATQLHATSTQMSTGAEEVAQQAATVATASEEMAATSAE
IAHNCSLAAESSRHANDRAENGSDVVQETLTVMNRIAERVKDSARTVESLGERSDQIGEIIGTIQDIADQTNLLALNAAI
EAARAGEQGRGFAVVADEVRALAERTTKATKEISQMIKAIQGETKGAVTSMEEGVKEVEKGTSDASKSGEALQAILEQIG
GVTMQVSQIATAAEEQTATTGEINNNIQQITEVVQLTARGAEESAQAAEQLAKLAEELQDLVYKFKLA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=309, Percent_Identity=32.6860841423948, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787690, Length=550, Percent_Identity=26.7272727272727, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1788195, Length=296, Percent_Identity=32.0945945945946, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788194, Length=363, Percent_Identity=30.5785123966942, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789453, Length=355, Percent_Identity=27.3239436619718, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 58110; Mature: 57978

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCC
>Mature Secondary Structure 
SAWRDLKVRTKIFVLVIAGCLGLVVLGSVALYNMRNLSGSVKEANIGMEHVAGLSGMKS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHCCHHH
DFLEMRLALVYMLALKDAEKIGGKEQDFLKAADRIKKTLDDLGKQELTDTEKKSLVEFRG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHC
GFESYVEKGTRLAELIKDATAKGDEVGRADAMTFATQSVAPLYDTPAKIIASMVQENIGE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AHKMYEQDMASYRASFIMMVVIILGVIGVAAAAGLAIAGSISGPLNKVLDVLTRVAAGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TARADVVSADEMGLLAREVNTTAAKINEIIGLVAHNASQVTAAATQLHATSTQMSTGAEE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHH
VAQQAATVATASEEMAATSAEIAHNCSLAAESSRHANDRAENGSDVVQETLTVMNRIAER
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VKDSARTVESLGERSDQIGEIIGTIQDIADQTNLLALNAAIEAARAGEQGRGFAVVADEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHH
RALAERTTKATKEISQMIKAIQGETKGAVTSMEEGVKEVEKGTSDASKSGEALQAILEQI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
GGVTMQVSQIATAAEEQTATTGEINNNIQQITEVVQLTARGAEESAQAAEQLAKLAEELQ
CCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DLVYKFKLA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]