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The map label for this gene is nifV [H]

Identifier: 39996040

GI number: 39996040

Start: 1007092

End: 1008243

Strand: Direct

Name: nifV [H]

Synonym: GSU0937

Alternate gene names: 39996040

Gene position: 1007092-1008243 (Clockwise)

Preceding gene: 39996039

Following gene: 39996041

Centisome position: 26.4

GC content: 64.32

Gene sequence:

>1152_bases
ATGGAACATACCCTTAAGCACGAAGTCATCATCGACGACACCACTCTGCGTGACGGCGAACAGACCGCCGGGGTCGTCTT
CAGCAAGAAAGAGAAGATAGCCATTGCCCGCATGCTTGACTCCATCGGGGTCCGGGAGTTGGAGTGCGGCATTCCGGCCA
TGGGAGAGGACGAACGCGCCGCGGTCCGGGCGCTGGTGGACCTGGGGCTCTCTGCCCGGCTCATTACCTGGAACCGGGCG
CTGGTGGCCGACATCCAGGCGTCCATCGACTGCGGCATTACCGCGGTGGACATCTCCCTGTCGGTTTCCGACCAGATGAT
CGCCCACAAGTTGCGCAAGGACCGCCGTTGGGTAAAGGAGCAGCTCAGGCACTCCCTCGGCTTTGCGAAGGAGAAGGGGC
TTTACGTTTCAGTGGGAGGGGAGGATTCGAGCCGGGCCGACCTCCTCTTCCTGGTGGAGCTCATGGAGGTCGCCCGCGAT
ATGGGAGCCGACCGGTTCCGCTTTTGCGACACCCTCGGCATTCTCGACCCCTTCACCATGTACGAAAAGGTGCGCACGCT
ACGAACGGCCGTGCCGGAACTCGATATCGAAGTGCACACCCACAACGACCTGGGGATGGCCACCGCCAATGCAATCGCCG
GAATCAAGGCCGGTGCCCGTTTTGTGAACACCACCGTAAACGGCCTTGGCGAACGGGCCGGCAACGCGGCTCTGGAAGAG
GTGGTCATGGCGCTCAAGCTCGCCTGCGGGTGTGATCCGGGCATCGACACCCAACGCTTCCATGAGATCTCGCGCTTCGT
GGGGCGTGCCTCCCAGCGGCCGGTCCCTCCCTGGAAAGCGGTGGTGGGCGAGCGGGTCTTTTCTCACGAATCGGGGCTCC
ACGCGGACGGCGTCATCAAGAACCCCCTCAATTACGAGGGATTCGATCCGGCGGAGGTGGGGCTTCGGCGCCACATCGTG
GTGGGCAAGCATTCGGGGACGAGCGGTCTCGTGGAGCGGTTCCGGAGCATGGGGGTGGAACTGGCGCGCGAAGAGGCCGC
GGAGCTGATGGATGAGGTGCGCGCCGTGGCCCAGCGGCTCAAGCGCCCCCTCTCCGACGGCGATCTCCGCCGGCTCTACG
CGGCGGGAAAGCATGACCGCCTGGCAGCATGA

Upstream 100 bases:

>100_bases
TAATCTAAACGTACATAACCGACAGATTCAGTCTTGCAGGGCATCGGGGCCCCGGAACAGCATTCCGGGGCCTTTTCGTT
TGCCGGAAAGGAGCCGGGCC

Downstream 100 bases:

>100_bases
TGCGCTGATGTTCATGGTTGCATAAATAATGGGCAAACGAGGGGGCGGGCGATCGGCGTGCCCCTTCTTTTCTGGTCGGC
AAAATTATCGAAGTAACAAT

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MEHTLKHEVIIDDTTLRDGEQTAGVVFSKKEKIAIARMLDSIGVRELECGIPAMGEDERAAVRALVDLGLSARLITWNRA
LVADIQASIDCGITAVDISLSVSDQMIAHKLRKDRRWVKEQLRHSLGFAKEKGLYVSVGGEDSSRADLLFLVELMEVARD
MGADRFRFCDTLGILDPFTMYEKVRTLRTAVPELDIEVHTHNDLGMATANAIAGIKAGARFVNTTVNGLGERAGNAALEE
VVMALKLACGCDPGIDTQRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIKNPLNYEGFDPAEVGLRRHIV
VGKHSGTSGLVERFRSMGVELAREEAAELMDEVRAVAQRLKRPLSDGDLRRLYAAGKHDRLAA

Sequences:

>Translated_383_residues
MEHTLKHEVIIDDTTLRDGEQTAGVVFSKKEKIAIARMLDSIGVRELECGIPAMGEDERAAVRALVDLGLSARLITWNRA
LVADIQASIDCGITAVDISLSVSDQMIAHKLRKDRRWVKEQLRHSLGFAKEKGLYVSVGGEDSSRADLLFLVELMEVARD
MGADRFRFCDTLGILDPFTMYEKVRTLRTAVPELDIEVHTHNDLGMATANAIAGIKAGARFVNTTVNGLGERAGNAALEE
VVMALKLACGCDPGIDTQRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIKNPLNYEGFDPAEVGLRRHIV
VGKHSGTSGLVERFRSMGVELAREEAAELMDEVRAVAQRLKRPLSDGDLRRLYAAGKHDRLAA
>Mature_383_residues
MEHTLKHEVIIDDTTLRDGEQTAGVVFSKKEKIAIARMLDSIGVRELECGIPAMGEDERAAVRALVDLGLSARLITWNRA
LVADIQASIDCGITAVDISLSVSDQMIAHKLRKDRRWVKEQLRHSLGFAKEKGLYVSVGGEDSSRADLLFLVELMEVARD
MGADRFRFCDTLGILDPFTMYEKVRTLRTAVPELDIEVHTHNDLGMATANAIAGIKAGARFVNTTVNGLGERAGNAALEE
VVMALKLACGCDPGIDTQRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIKNPLNYEGFDPAEVGLRRHIV
VGKHSGTSGLVERFRSMGVELAREEAAELMDEVRAVAQRLKRPLSDGDLRRLYAAGKHDRLAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=385, Percent_Identity=33.5064935064935, Blast_Score=187, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6320071, Length=361, Percent_Identity=31.0249307479224, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6320019, Length=364, Percent_Identity=31.3186813186813, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324682, Length=413, Percent_Identity=25.6658595641646, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324225, Length=414, Percent_Identity=25.3623188405797, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582381, Length=299, Percent_Identity=25.752508361204, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42082; Mature: 42082

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHTLKHEVIIDDTTLRDGEQTAGVVFSKKEKIAIARMLDSIGVRELECGIPAMGEDERA
CCCCCCCEEEEECCCCCCCCHHCCEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHH
AVRALVDLGLSARLITWNRALVADIQASIDCGITAVDISLSVSDQMIAHKLRKDRRWVKE
HHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
QLRHSLGFAKEKGLYVSVGGEDSSRADLLFLVELMEVARDMGADRFRFCDTLGILDPFTM
HHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
YEKVRTLRTAVPELDIEVHTHNDLGMATANAIAGIKAGARFVNTTVNGLGERAGNAALEE
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VVMALKLACGCDPGIDTQRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
NPLNYEGFDPAEVGLRRHIVVGKHSGTSGLVERFRSMGVELAREEAAELMDEVRAVAQRL
CCCCCCCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KRPLSDGDLRRLYAAGKHDRLAA
HCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEHTLKHEVIIDDTTLRDGEQTAGVVFSKKEKIAIARMLDSIGVRELECGIPAMGEDERA
CCCCCCCEEEEECCCCCCCCHHCCEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHH
AVRALVDLGLSARLITWNRALVADIQASIDCGITAVDISLSVSDQMIAHKLRKDRRWVKE
HHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
QLRHSLGFAKEKGLYVSVGGEDSSRADLLFLVELMEVARDMGADRFRFCDTLGILDPFTM
HHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHH
YEKVRTLRTAVPELDIEVHTHNDLGMATANAIAGIKAGARFVNTTVNGLGERAGNAALEE
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VVMALKLACGCDPGIDTQRFHEISRFVGRASQRPVPPWKAVVGERVFSHESGLHADGVIK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
NPLNYEGFDPAEVGLRRHIVVGKHSGTSGLVERFRSMGVELAREEAAELMDEVRAVAQRL
CCCCCCCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KRPLSDGDLRRLYAAGKHDRLAA
HCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]