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The map label for this gene is nasB [H]

Identifier: 39996012

GI number: 39996012

Start: 973639

End: 974862

Strand: Reverse

Name: nasB [H]

Synonym: GSU0909

Alternate gene names: 39996012

Gene position: 974862-973639 (Counterclockwise)

Preceding gene: 39996013

Following gene: 39996011

Centisome position: 25.56

GC content: 67.24

Gene sequence:

>1224_bases
GTGGAACGGCACTTCGACATCGTCATCATCGGCAACAGCGCGGCGGGAATGCAGGCCCTGCGCACCATCCGCAGGCATTC
CCGCAGCGTATCCGTAGCCATGGTCGACCGGGAGAACTGTCCGGCCTACTCAAGGGTCCTGACCCCCTACTATGTTGGCG
GCAAGACCCTTCGGGGAAACCTTTTCATCGTGGGAAGCGATTTCTACGACCACTACGGTATCGCCACCCTCCTTGGCCAT
GCTGCAGTGGAACTGGATGCGGAACGGCACCGTCTCCTCCTGGCCGACGGCACGGCGGTGGGGTTCGGGCAACTCCTCAT
TGCCGCCGGGGCCGAAGCCCGGGGACTGGAGGTGACCGCCGAGGGGGTCTGCACCCTGCGGCACCTGGCCGACGCCGACC
GGTTGGAGCGGCTCCTGAAGGGGGCCCGAAGCGTCACCGCCGTGGGCGCGGGACTTGTGAGCATCCCGCTGCTCTCCCAT
GCGGGACCGGAGGTGGAGCGGCACCTGGTGATCGGCTCGGATCGGGTCTTCAGCCGGGTGGTGGACCCTGAGGCATCCGC
CATCCTGGAGGAGCGCTTCCTGGCAGACGGCCTTGTCCTTCACAAACGGGACGACATCGTGAACCTGTCCGGCACTGACC
GGCTGGAGCTCTCCCTGGCCACGGGCAAACGGATCGTCACCGACATGCTGCTGGTAGGCAAGGGGGTGGTTCCCAACACT
GACCTGGCCCTCCGGGCGGGGTTGGAAGTGCGGGAGGGCATCGTGACGGACGAGCGCTGCCGCACCAGCCACCCCCAGAT
TTTCGCTGCCGGTGACGTGGCCGAGGGAAAGGACTTCGTCACCGGAGAGCCGACCATCCAGGGCAACTGGCTGACCGCGG
TGGAGCAGGGGGAGATCGCGGCCCTGAATATGCTCGGCTTCGACTGTGCCTACGAGGGGAGCCTCAAGAACAACACCACC
GAGGTCTTCGGCGTGGATGTGGCCGTGGTGGGCTACTGCCGCGACGACGCCCCCCGAACCGCCACGGCTCGCGACCCGTT
CACCGGCCGGTTCCGCAAGGTCTTTCTGGACGGGCGCGACCGCGTCATCGGCGCCACCATGATCGGCGAGACCAACGACG
CCGGCCTCTACTATCAACTGGTCAGAACCCGCGCCCTCTTCCCCGGCCCCACCTTCCTGCGGGGAACCGGCACCCAGGCC
GCGGCCCTGGCCCGGCTTGCCTGA

Upstream 100 bases:

>100_bases
CGGCTCGCCGAGATGCTCCCCGACTACTACCGGGCCCGGGGCTGGGACGAACAGGGCATTCCCACGGATGAGAAGTTGAA
GTCACTGGGACTCGCCTGAC

Downstream 100 bases:

>100_bases
TGCCCATGGTCAGCGTCCGGTTCTACAGTCTCCTGCGCCTGATGCTCGGGCGCGAAGTCGTGGAACTCCCCTGGGAGGCG
GGGGAGACGGTCCGAAGCCT

Product: pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MERHFDIVIIGNSAAGMQALRTIRRHSRSVSVAMVDRENCPAYSRVLTPYYVGGKTLRGNLFIVGSDFYDHYGIATLLGH
AAVELDAERHRLLLADGTAVGFGQLLIAAGAEARGLEVTAEGVCTLRHLADADRLERLLKGARSVTAVGAGLVSIPLLSH
AGPEVERHLVIGSDRVFSRVVDPEASAILEERFLADGLVLHKRDDIVNLSGTDRLELSLATGKRIVTDMLLVGKGVVPNT
DLALRAGLEVREGIVTDERCRTSHPQIFAAGDVAEGKDFVTGEPTIQGNWLTAVEQGEIAALNMLGFDCAYEGSLKNNTT
EVFGVDVAVVGYCRDDAPRTATARDPFTGRFRKVFLDGRDRVIGATMIGETNDAGLYYQLVRTRALFPGPTFLRGTGTQA
AALARLA

Sequences:

>Translated_407_residues
MERHFDIVIIGNSAAGMQALRTIRRHSRSVSVAMVDRENCPAYSRVLTPYYVGGKTLRGNLFIVGSDFYDHYGIATLLGH
AAVELDAERHRLLLADGTAVGFGQLLIAAGAEARGLEVTAEGVCTLRHLADADRLERLLKGARSVTAVGAGLVSIPLLSH
AGPEVERHLVIGSDRVFSRVVDPEASAILEERFLADGLVLHKRDDIVNLSGTDRLELSLATGKRIVTDMLLVGKGVVPNT
DLALRAGLEVREGIVTDERCRTSHPQIFAAGDVAEGKDFVTGEPTIQGNWLTAVEQGEIAALNMLGFDCAYEGSLKNNTT
EVFGVDVAVVGYCRDDAPRTATARDPFTGRFRKVFLDGRDRVIGATMIGETNDAGLYYQLVRTRALFPGPTFLRGTGTQA
AALARLA
>Mature_407_residues
MERHFDIVIIGNSAAGMQALRTIRRHSRSVSVAMVDRENCPAYSRVLTPYYVGGKTLRGNLFIVGSDFYDHYGIATLLGH
AAVELDAERHRLLLADGTAVGFGQLLIAAGAEARGLEVTAEGVCTLRHLADADRLERLLKGARSVTAVGAGLVSIPLLSH
AGPEVERHLVIGSDRVFSRVVDPEASAILEERFLADGLVLHKRDDIVNLSGTDRLELSLATGKRIVTDMLLVGKGVVPNT
DLALRAGLEVREGIVTDERCRTSHPQIFAAGDVAEGKDFVTGEPTIQGNWLTAVEQGEIAALNMLGFDCAYEGSLKNNTT
EVFGVDVAVVGYCRDDAPRTATARDPFTGRFRKVFLDGRDRVIGATMIGETNDAGLYYQLVRTRALFPGPTFLRGTGTQA
AALARLA

Specific function: Required for nitrate assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=341, Percent_Identity=26.3929618768328, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI226437568, Length=341, Percent_Identity=26.3929618768328, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21389617, Length=341, Percent_Identity=26.3929618768328, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788892, Length=216, Percent_Identity=31.9444444444444, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789765, Length=398, Percent_Identity=22.8643216080402, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789065, Length=312, Percent_Identity=26.9230769230769, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=389, Percent_Identity=24.4215938303342, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564386, Length=352, Percent_Identity=27.5568181818182, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28573993, Length=355, Percent_Identity=25.0704225352113, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581020, Length=355, Percent_Identity=25.0704225352113, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585130, Length=397, Percent_Identity=22.4181360201511, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24639257, Length=310, Percent_Identity=23.5483870967742, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281359715, Length=310, Percent_Identity=23.5483870967742, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281359713, Length=310, Percent_Identity=23.5483870967742, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639250, Length=310, Percent_Identity=23.5483870967742, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18543267, Length=310, Percent_Identity=23.5483870967742, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24639252, Length=310, Percent_Identity=23.5483870967742, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43797; Mature: 43797

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERHFDIVIIGNSAAGMQALRTIRRHSRSVSVAMVDRENCPAYSRVLTPYYVGGKTLRGN
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCEEEECCEEEECC
LFIVGSDFYDHYGIATLLGHAAVELDAERHRLLLADGTAVGFGQLLIAAGAEARGLEVTA
EEEEECCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCHHHEEEECCCCCCCEEEEH
EGVCTLRHLADADRLERLLKGARSVTAVGAGLVSIPLLSHAGPEVERHLVIGSDRVFSRV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCHHHHHHH
VDPEASAILEERFLADGLVLHKRDDIVNLSGTDRLELSLATGKRIVTDMLLVGKGVVPNT
CCCCHHHHHHHHHHHCCEEEEECCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
DLALRAGLEVREGIVTDERCRTSHPQIFAAGDVAEGKDFVTGEPTIQGNWLTAVEQGEIA
CCEEECCCHHHHCCCCCHHHCCCCCCEEEECCCCCCCCEECCCCEECCCEEEEEECCCEE
ALNMLGFDCAYEGSLKNNTTEVFGVDVAVVGYCRDDAPRTATARDPFTGRFRKVFLDGRD
EEEEECCCEEECCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCC
RVIGATMIGETNDAGLYYQLVRTRALFPGPTFLRGTGTQAAALARLA
EEEEEEEECCCCCCCHHHEEHHHHHCCCCCCEEECCCCHHHHHHHCC
>Mature Secondary Structure
MERHFDIVIIGNSAAGMQALRTIRRHSRSVSVAMVDRENCPAYSRVLTPYYVGGKTLRGN
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCEEEECCEEEECC
LFIVGSDFYDHYGIATLLGHAAVELDAERHRLLLADGTAVGFGQLLIAAGAEARGLEVTA
EEEEECCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCHHHEEEECCCCCCCEEEEH
EGVCTLRHLADADRLERLLKGARSVTAVGAGLVSIPLLSHAGPEVERHLVIGSDRVFSRV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCHHHHHHH
VDPEASAILEERFLADGLVLHKRDDIVNLSGTDRLELSLATGKRIVTDMLLVGKGVVPNT
CCCCHHHHHHHHHHHCCEEEEECCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
DLALRAGLEVREGIVTDERCRTSHPQIFAAGDVAEGKDFVTGEPTIQGNWLTAVEQGEIA
CCEEECCCHHHHCCCCCHHHCCCCCCEEEECCCCCCCCEECCCCEECCCEEEEEECCCEE
ALNMLGFDCAYEGSLKNNTTEVFGVDVAVVGYCRDDAPRTATARDPFTGRFRKVFLDGRD
EEEEECCCEEECCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCC
RVIGATMIGETNDAGLYYQLVRTRALFPGPTFLRGTGTQAAALARLA
EEEEEEEECCCCCCCHHHEEHHHHHCCCCCCEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]